Counting Colonies; Quick Capture - UVP ColonyDoc-It Instruction Manual

ColonyDoc-It Imaging Station

Counting Colonies

Quick Capture

Place the Petri dish to be counted on the transillumination surface.
Select the appropriate lighting option for the sample. If unsure, turn the selector switch (located on the front of the
colony counter) to Base Lighting > Trans and remove the black plate (if present). The Trans lighting option will
apply to most applications.
If the colony counter is not already open, double click onto the Doc-It Colony Counter icon on the desktop.
Ensure that the Camera Control module is active. See the image of the screen below noted by the green circle.
The text must be black as shown. If the Camera Control is inactive (with grey text) proceed to the troubleshooting
section: Inactive Camera Window.
Select the Petri dish plate diameter from the Camera Control module. The choices are 6cm, 10cm, and 15cm.
Select the smallest colony size for this plate. The choices are <1mm and 1mm+. If <1mm is selected the
smallest colony size is below 1mm. The count will be faster if 1mm+ is chosen.
Choose the White Light option from Colony Lighting. The Dim to Bright slider will be disabled unless using the
Fluorescent Colony Lighting option. If counting fluorescent colonies go to the Fluorescent Colony Counting
section of the manual.
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