Fluorescent Colony Counting - UVP ColonyDoc-It Instruction Manual

ColonyDoc-It Imaging Station
The new window will display the Total colonies
counted.

Fluorescent Colony Counting

Ensure that the doors are attached to the
ColonyDoc-It or turn off the surrounding lights.
Turn the Epi Lighting switch on the ColonyDoc-It
to Blue.
Insert the Green Fluorescent Protein (GFP)
emission filter into either tray (refer to the
Installing the Filter section of this manual for
instructions) and ensure that the filter is positioned
below the camera. (If the GFP filter is in filter
position one, the filter slider should be moved all
the way to the right position.)
Insert the fluorescently stained sample into the
ColonyDoc-It.
Select the appropriate Plate Diameter, the
Smallest Colony information and choose the
Fluorescent lighting function of Colony Lighting
in the Doc-It Colony Counter software.
The slider bar may be adjusted from Dim to
Bright to provide optimum results.
Select Preview to position the plate inside the red
circle guides and then select Capture when the
image is positioned properly.
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