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Related Manuals for Knauer Axura DAD 6.1L
Summary of Contents for Knauer Axura DAD 6.1L
- Page 1 Detector DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700...
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Page 2: Table Of Contents
Table of Contents Table of Contents Note For your own safety, read the manual and always observe the warnings and safety information on the device and in the manual! Intended Use ............Device Overview . - Page 3 Table of Contents Operation ............Initial Start Up .
- Page 4 +49 30 8015010 Thank you. A qui que ce soit Si jamais vous préfériez un manuel en francais pour ce poduit contacter KNAUER par email ou par fax avec le no. de série: support@knauer.net +49 30 8015010 Merci beaucoup.
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Page 5: Intended Use
Intended Use Intended Use Note: Only use the device for applications that fall within the range of the intended use. Otherwise, the protective and safety equipment of the device could fail. Description The detector can be used for analytical, semi-preparative and preparative applications. -
Page 6: Side View
Intended Use Side View On the side of the detector, you find the connector for the operating panel Control Unit in chapter ’Operating with Control Unit’ on page 26. Legend USB port for the Control Unit Fig. 3 USB port for the Control Unit Features In an HPLC system, the detector serves to detect substances in liquids and show their concentration. -
Page 7: Eluents
Note: The list of selected solvents was compiled based on research in the pertinent literature and is only a recommendation by KNAUER. If there is any doubt, contact the Technical Support of the manufacturer. -
Page 8: Less Suitable Eluents
Scope of Delivery sodium hydroxide (1 M) water Less Suitable Eluents diethylamine (0.1 %) (DEA) dimethyl sulfoxide (DMSO) slightly volatile eluents methylene chloride tetrahydrofuran (THF) phosphoric dilute acid triethylamine (0.1 %) (TEA) ... -
Page 9: Safety For Users
Only use a given PEEK fitting for one specific port and never re-use it for other ports. Always install new PEEK fittings on each separate port. Follow KNAUER or manufacturer's instructions on caring for the columns More safety-relevant information is listed below: ... -
Page 10: Signal Words
Decontamination Report Devices without a completed Decontamination Report will not be repaired. If you would like to return a device to KNAUER, make sure to enclose a com- pleted Decontamination Report with the device: http://www.knauer.net/ en/downloads/service.html... -
Page 11: Symbols And Signs
Transport Carefully prepare the device for transport or storage. If you want to return your device to KNAUER for repairs, enclose the Service Request Form which can be downloaded from our website. Device Data For a secure transport, note the weight and dimensions of the device (see Technical Data). -
Page 12: Operating Environment
Installation Operating Environment Only if the requirements for ambient conditions of the operating environ- ment are met, can the intended use be ensured. Details on the operating conditions can be found in the Technical Data section. Device defect The device overheats at exposure to sunlight and insufficient air circulation. Device failures are very likely. -
Page 13: Inserting The Flow Cell
Next Steps Connect the capillaries. Inserting the Flow Cell with Fiber Optics KNAUER fiber optic cables are extremely robust. Nevertheless fiber optic cables are sensitive to bending. The minimum bending radius is 100 mm (short term: 70 mm). Observe the following regarding the use of fiber optics: ... -
Page 14: Connecting The Capillaries
Prerequisites The flow cell was inserted. Screw-type cap fittings and cap fittings were removed. Note: The KNAUER LightGuide flow cell cartridges have unisex input and output connections. Component defect Damage to the ports caused by strongly tightened fittings. ... -
Page 15: Using The Peek Fittings
Installation Process Process Figure 1. Push the capillary through the fitting 2. Slide the lock ring over the tubing. Note that the tapered end of the lock ring has to point to the seal ring 3. Attach the seal ring Fig. -
Page 16: Control
Installation Process Process Figure 3. Connect the nozzle and the fun- nel. 4. Push the other end of the hose onto the nozzle of the leak tray. Fig. 14 Hose connected to device 5. For the bottom device, push the short end of the nozzle into the opening in the collection... -
Page 17: Connecting The Device To The Computer
Installation USB port for the Control Unit Fig. 17 USB port for the Control Unit Connecting the Device to the Computer This section describes how to set up an HPLC system in a local area network (LAN) and how a network administrator can integrate this LAN into your company network. -
Page 18: Configuring The Router
Installation You will find patch cables for each device and the router in the accessories kit. To connect the router to a WAN, an additional patch cable is required, which is not supplied within the scope of delivery. Prerequisite The computer has been switched off. -
Page 19: Setting A Static Ip Address
Installation 2. Enter the port number in the chromatography software. Result The connection is established. Setting a Static IP Address A static IP address can be set via the Mobile Control. Note: Please inform yourself about the IT safety standards valid for your lab- oratory, before changing the LAN settings. -
Page 20: Connections To The Terminal Strip
Installation The following remote signals can be received and sent: for receiving start, control, and error signals from external devices for sending start, control and error signals to external devices Fig. 18 relation between display and terminal strip Connections to the Terminal Strip Connection Function... -
Page 21: Connecting Cables To The Terminal Strip
Installation Connection Function Start IN TTL Input Low active Secure switching threshold at least 10 mA After receiving a signal (short-circuit to ground) from an external device, the device starts. If con- trolled with software, an electronic trigger is send through the LAN. -
Page 22: Integrator Connector
Only the supplied power cable is to be used to connect the device to the mains supply. Replace defective power cables only with accessories from KNAUER. Only use power cables with a permission for use in your country. In case of queries contact the Technical Support. -
Page 23: Integrating The Detector Into The System
Operation Integrating the Detector into the System To integrate the detector into a system, note the ambient conditions found in the section Technical Data as well as the ambient conditions of other devices to be integrated into that system. The detector is integrated into the HPLC flow system by connecting the capillary to the flow cell and the HPLC system. -
Page 24: Switch On
If the device is part of an HPLC system, you should also note the following: The network connection to the router is established The chromatography software has been installed by KNAUER or a com- pany authorized by KNAUER. A flow cell was inserted. -
Page 25: Operating With Control Unit
Operation You find a detailed description on chromatography software in the corre- sponding user manual. Operating with Control Unit The Control Unit is an optional accessory. To control the device using the Control Unit, connect the cable of the Control Unit to the device. You find a detailed description on the Control Unit in its accompanying user manual. -
Page 26: Default Settings
Optimizing the Detector Color Status Operation green The deuterium lamp is active. Right LED green The device has been switched on. flashes green The device not ready Wait until the for operation. device is ready for operation. blue The device is in Press the standby standby... -
Page 27: Warm Up Time
Optimizing the Detector Warm Up Time The recommended warm-up time for the detectors is 30 minutes. This rec- ommendation is applicable after turning the detector on as well as and after turning the lamp/s on. Fig. 22 cold start temperature profile Selecting the Flow Cell Several different flow cells are available for the detector (see “Flow Cells”... -
Page 28: Path Length
Optimizing the Detector Cartridge flow cells with volumes of 2 μl, 6 μl and 10 μl are available for the detectors. Narrow-bore columns (~ 2,1 mm ID) are suitable for flow cells with smaller volumes. Columns with with a larger inner diameter (≥ 3,0 mm ID) are less affected by the volume of the flow cell. -
Page 29: Connection
Optimizing the Detector Connection Undesired effects, like the loss of resolution in the chromatogram, may be prevented by ensuring a correct connection to the flow cell and the removal of any dead volume. Remote Operation (Fiber Optics) If the flow cell must be positioned outside of the detector (e.g. in an explo- sion-proof room, at higher temperatures such as in an oven, or in an envi- ronment with radioactive substances), the devices can be optionally equipped with fiber optic connectors. -
Page 30: Bandwidth
Optimizing the Detector Bandwidth The bandwidth defines the total number of wavelengths actually registered by the photodiode when a specific wavelength is set. For example, a wave- length set at 254 nm width a bandwidth of 4 nm results in average absorp- tion of 252–256 nm. -
Page 31: Integration Time
Optimizing the Detector Peak Width Time Constant Data Rate [min] [Hz] <0.003 0.01 >0.007 0.02 >0.017 0.05 >0.033 >0.067 >0,167 >0,333 Data Rate The data rate (or sampling rate) is the number of data points per second (Hz) at which the detector transmits data to the computer. Default Data Rate The default data rate setting for the detectors is 1 Hz (see “Default Settings”... -
Page 32: Subtraction Of The Baseline Chromatogram
Optimizing the Detector The integration time is automatically calculated by the software before the start of a measurement. The calculations are in relation to the special range (see “Spectral Range” on page 31). When a narrower spectral range is selected, the signal intensity will be increased. -
Page 33: Functionality Tests
Functionality Tests Installation Qualification The customer may request the Installation Qualification, which is free of charge. In case of a request, the Technical Support of KNAUER or from a (IQ) provider authorized by KNAUER performs this functionality test during the installation. -
Page 34: Troubleshooting
Troubleshooting Troubleshooting First Measures 1. Check all cabling. 2. Check all screw fittings. 3. Check whether air has gotten into the supply lines. 4. Check device for leaks. 5. Pay attention to system messages. Go through the following steps, in case no connection between the com- puter and the devices can be established. -
Page 35: System Messages
Switch off lamp and turn on again. In case the sys- start” tem message repeats itself, inform the Technical Support of KNAUER . The lamp unit has to be replaced. No lamp ignition when starting the device or the manual validation. - Page 36 Mount the lamp cover. In case the system mes- sage repeats itself, inform the Technical Support of KNAUER . “Lamp not installed” GLP data can not be read and lamp operation can not be executed, as the lamp (D2 or halogen) has not been installed.
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Page 37: Maintenance And Care
Opening the Module The device may only be opened by the Technical Support of KNAUER or any company authorized by KNAUER. Electric shock Danger of electric shock from voltage-carrying parts inside the device. The housing serves as a protective cover against voltages inside the device. -
Page 38: Contacting The Technical Support
Maintenance Contract The following maintenance work on the device may only be performed by KNAUER or a company authorized by KNAUER and is covered by a separate maintenance contract: • Opening the device or removing housing parts. Maintenance Intervals... -
Page 39: Cleaning And Caring For The Device
Maintenance and Care Cleaning and Caring for the Device Flow Cell Cartridge An important aspect in handling your flow cell cartridge, is that you should not touch the fiber optic ends with your fingers. Your finger may leave a thin fat layer on the fiber optic ends, which drastically impairs the flow cell’s and detector’s performance (see “Possible Problems and Rectifications”... -
Page 40: Storage
Maintenance and Care Process Process 1. Unscrew the eluent supply lines and close the open connectors with hole plugs. 2. Disconnect the detector from the system and close the open connec- tor of the flow cell with a hole plug. Next Steps Select a storage location according to the requirements, which are listed in the according chapter of this manual. -
Page 41: Advanced Cleaning
Maintenance and Care Advanced Cleaning This section describes a cleaning procedure for aggressive cleaning of Light- Guide flow cells. Preparation of Chemicals All chemical reagents should be of at least ACS-Grade, preferably HPLC- Grade. This procedure involves the use of caustic and flammable reagents (see “Safety for Users”... -
Page 42: Replacing The Flow Cell
Maintenance and Care Final Rinsing Process 1. Identify the point where subsequent cleaning cycles no longer improve the performance of the flow cell. 2. Flush the unit with ultrapure water for a period of at least 15 minutes to ensure all cleaning solutions have been completely removed and there are no persistent residues that might affect flow cell performance or stability. -
Page 43: Removing The Deuterium Or Halogen Lamp
Maintenance and Care Legend deuterium lamp con- nector halogen lamp connec- tor (DAD 6.1L) halogen lamp (DAD 6.1L). deuterium lamp Fig. 31 overview deuterium lamp and halogen lamp Removing the Deuterium or Halogen Lamp Prerequisites The device is switched off. ... -
Page 44: Installing The Deuterium Or Halogen Lamp
Maintenance and Care Process Process Figure 3. Loosen the lock ring the lamp plug and pull out the plug. Fig. 33 lock ring of lamp plug 4. Using the screwdriver, loosen the two screws 5. Remove the lamp. Fig. 34 screws on the lamp socket Next Steps Insert a new lamp. -
Page 45: Removing The Leak
Technical Data Process Process Figure 4. Using the screwdriver, tighte the two screws on the lamp socket. 5. Connect the plug tighten the lock ring. Fig. 37 installing the deuterium lamp 6. Attach the lamp cover and fix it with the screws Fig. -
Page 46: Dad 2.1L
Technical Data wavelength verifica- internal holmium filter and deuterium lines tion wavelength preci- ≤ 0.5 nm sion noise ± 3.5 μAU at 254 nm drift 300 μAU/h at 254 nm linearity > 2.0 AU at 274 nm, typically 2.5 AU time constants 0.0 / 0.1 / 0.2 / 0.5 / 1.0 / 2.0 / 5.0 / 10.0 s integration time... - Page 47 Technical Data wavelength range 190–700 nm spectral bandwidth < 8 nm at Hα line (FWHM) Note: digital bandwidth 1–32 nm wavelength accu- ± 1 nm racy wavelength preci- ± 0.1 nm sion wavelength verifica- internal holmium filter and deuterium lines tion noise ±...
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Page 48: Mwd 2.1L
Technical Data MWD 2.1L Detection detector type multiwavelength detector detection channels 8 (digital), 4 (analog) light source deuterium (D ) lamp with integrated GLP chip wavelength range 190–700 nm spectral bandwidth < 8 nm at Hα line (FWHM) Note: digital bandwidth 1–32 nm wavelength accu- ±... -
Page 49: Conditions For Specification
packaging and transport damage In the event of device malfunctions, directly contact the manufacturer. KNAUER Wissenschaftliche Geräte GmbH Hegauer Weg 38 14163 Berlin, Germany Phone: +49 30 809727-111... -
Page 50: Warranty Seal
2001), old devices manufactured by KNAUER are marked as waste electrical and electronic equipment: 160214. WEEE Registration KNAUER as a company is registered by the WEEE number DE 34642789 in the German "Elektroaltgeräteregister" (EAR). The number belongs to cate- gory 8 and 9, which, among others, comprise laboratory equipment. - Page 51 Luer-lock glass syringe, 10 ml A0574 Flow Cells Technical Data Order Number Standard path length 10 mm AMC19 KNAUER LightGuide connection 1/16“ Flow Cell Cartridge volume 2 μl (0.8 μl dispersion vol.) wetted parts PEEK, quartz, Teflon max. flow rate 5 ml/min max.
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Page 52: Flow Cells
Repeat Orders Technical Data Order Number High Sensitivity path length 50 mm AMD59 KNAUER LightGuide connection 1/16“ Flow Cell Cartridge volume 6 μl (2 μl dispersion vol.) wetted parts PEEK, quartz, Teflon max. flow rate 5 ml/min max. pressure 50 bar... -
Page 53: Fiber Optic Cables
Repeat Orders Technical Data Order Number path length 0.5 mm A4096 connection 1/16“ volume 3 μl wetted parts PEEK, quartz, PTFE max. flow rate 250 ml/min max. pressure 100 bar path length 0.5 /1.25 /2 mm A4078 connection 1/8“ volume 1.7/4.3/6.8 μl wetted parts SSt, quartz, PTFE... -
Page 54: Hplc Glossary
HPLC Glossary HPLC Glossary In the following chapter you find abbreviations and terminology that is used in HPLC. Term Definition absorption The process of retention in which the solute partitions into a liquid-like coating. adsorption A process of retention in which the interactions between the solute an dthe surface of an adsorbent dominate. - Page 55 HPLC Glossary Term Definition retention time The time required from an injection of a substance until the maximum concentration of a substance becomes visible. sample A mixture of different components which are to be separated via chroma- tography. The components are moved by the mobile phase and dissolved from the column.
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Page 56: Index
Index Index disposal drift absorption accessories adapter eluents additives ambient conditions features analog port fiber optics analytical adapter AVV marking flow cell fittings backflushing flow cell bandwidth clean default connection Beer-Lambert law fiber optics insert material capillary path length fitting pressure stability lock ring rinse... - Page 57 Index port power supply problems pressure maximum router problems settings further measures setup professional group leak management rear view remove reference wavelength sensor default leak sensor refractive index remote connector Legal Information pin header location remote operation Luer-lock cannula repair repeat orders maintenance device...
- Page 58 Index Technical Support test Installation Qualification Operation Qualification warm up time time constant warranty transport damage wavelength troubleshooting cutoff select DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2...
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Page 59: Knauer
© KNAUER Wissenschaftliche Geräte GmbH All rights reserved. The information in this document is subject to change without prior notice. Original edition of this manual, version 3.2 Last update: 2016/10/13 Printed in Germany on environmentally friendly paper from sustainable forests.
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