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Summary of Contents for Knauer BlueShadow 50D
- Page 1 BlueShadow Detector 50D User Manual V7654 HPLC...
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Page 2: Table Of Contents
Table of Contents Table of Contents Note: For your own safety, read the manual and always observe the warn- ings and safety information on the device and in the manual! Intended Use ..........Device Overview . - Page 3 Table of Contents Program Menu ..........Creating a Program .
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Page 4: Table Of Contents
+49 30 8015010 Thank you. A qui que ce soit Si jamais vous préfériez un manuel en francais pour ce poduit contacter KNAUER par email ou par fax avec le no. de série: support@knauer.net +49 30 8015010 Merci beaucoup. -
Page 5: Intended Use
Intended Use Intended Use Device Overview The touchscreen, the LED light for standby mode, and the flow cell can be found on the detector's front side. Legend Touchscreen LED light for standby mode Flow cell with holder Fig. 1 Front view of the device The device can be operated from either the chromatography software at the workstation, or the touchscreen. -
Page 6: Features
Intended Use Laboratory Use Chemical analyses Biochemical analyses Chiral analyses Food analyses Pharmaceutical analyses Environmental analyses Features The detector is a measuring system for fast and precise measurement in the UV range and, with a halogen lamp (optional), also in the visible range. The measurement principle is based on the attenuation of a monochromatic light beam passing through liquid. -
Page 7: Eluents
(1M) Water valid for the specified temperature range Scope of Delivery Note: Only use original parts and accessories made by KNAUER or a com- pany authorized by KNAUER Delivery Detector with test cell User manual (EN/DE) Accessory Kit Transport protection 50D user manual V7654, version 2.0... -
Page 8: Safety
Only use a given PEEK fitting for one specific port and never re-use it for other ports. Always install new PEEK fittings on each separate port. Follow KNAUER or manufacturer's instructions on caring for the columns More safety-relevant information is listed below: ... -
Page 9: Signal Words
Device Securely off the power switch or by pulling the power plug. Opening the Device The device may be opened by the KNAUER Technical Support or any com- pany authorized by KNAUER only. Signal Words Possible dangers related to the device are divided into personal and material damage in this user manual. -
Page 10: Symbols And Signs
You have various options to contact the Technical Support: Phone +49 30 809727-111 +49 30 8015010 E-mail support@knauer.net You can make your requests in English and German. Location Requirements Requirements The location for the device must meet the following requirements: ... -
Page 11: Space Requirements
Only the supplied power cable is to be used to connect the device to the mains supply. Replace defective power cables only with original accessories from KNAUER. Only use power cables with a permission for use from your country. In case of queries contact the Technical Support. -
Page 12: Controlling With The Multi-Pin Connector
Unpacking and Setup Legend Serial number Integrator outlets LAN connection RS-232 port Fan opening Multi-pin connector Power switch Mains power connec- tion Fig. 3 Rear view of the device External devices can be connected to the detector in different ways: ... - Page 13 Unpacking and Setup Connection Function Start IN TTL input With a high level, a program is started in hold mode or a link with the corresponding wait parameter is started. Autozero Carry out zero balance. Reference point for the TTL level. +5 V Provides a voltage of 5 V with respect to GND.
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Page 14: Analog Control
The KNAUER chromatography software OpenLAB , ChromGate oder ® ClarityChrom has been installed by KNAUER or a company authorized by KNAUER. Capillaries from the column to the UV detector and capillaries from the detector to the waste bottle are securely attached. -
Page 15: Changing The Optical Path Length Of The Preparative Flow Cell
Initial Startup 8. Connect the incoming and outgoing capillaries at the inlet and outlet of the flow cell. Legend Inlet of the flow cell Outlet of the flow cell Knurled-head screws Cover plate Carriage Fig. 4 Flow cell Changing the optical path length of the preparative flow cell At delivery, the optical path length of a preparative flow cell is set to 2 mm at the factory. -
Page 16: Connecting The Capillaries
Initial Startup Extending the To extend the optical path length in steps of 0.75 mm, put the spacers back optical path length 1. Loosen the threaded ring. 2. Remove the stainless steel cover and fiber optics, together with the seal holder, with tweezers. -
Page 17: Configuring The Lan Settings
Initial Startup Note: To set up a LAN, we recommend to use a router. That means the fol- lowing steps are required: Process 1. On the computer, go to the control panel and check the LAN properties. 2. Hook up the router to the devices and the computer. 3. -
Page 18: Configuring The Router
Operating the Detector Process 1. Use the patch cable to connect the router and the computer. Repeat this step to connect all devices. 2. Use the power supply to connect the router to the mains power system. Configuring the Router The router is preset at the factory. -
Page 19: Switching The Detector On
Operating the Detector Switching the Detector on The device is switched by the power switch on the back of the device. It begins by initializing the lamps (initializing lamps), then proceeds through a self-test and calibration cycle (calibration is running), and finally registers itself as ready to operate with the status display. - Page 20 Operating the Detector Button Function Explanation To Main menu Tap to go to the Main menu Saves the entry Controlling the program Further buttons help control the program: Button Function Explanation Running a pro- Starts previously configured pro- gram gram in Program menu Pause program Stop program Other buttons have their functions displayed in plaintext:...
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Page 21: Graphical User Interface Structure
Operating the Detector Button Function Explanation Del/Delete Delete a program Table Program line display Autozero scan Note: The detector must perform the so-called autozero scan before record- ing UV spectra. Here the solvent's spectrum is measured, in order to correct the corresponding scan values. -
Page 22: Status Display Settings
Operating the Detector Detector 0.00127 λ: λ: Event Autozero Scan Start Menu Program Setup Link Scan Standby Setup Menu > Setup Menu > GLP Scan Network Event Instrument Validation Lamps Analog Date Lamps Analog T-const. Components Intensity Fraction Calibration Fig. 10 User interface structure (2/2) Status Display Settings The following settings can be made in the status display:... -
Page 23: Main Menu
Operating the Detector Main Menu Navigating the Tapping the button in the status display calls up the Main menu: Main menu Menu Program Setup Link Scan Standby Fig. 11 Main menu The individual menus are opened by tapping the correspondingly labeled buttons. -
Page 24: Creating Program Lines
Operating the Detector 2. Tap the desired program number and edit to edit the program. 3. Enter the desired signal options. 4. Enter the desired wavelengths and save them with 5. Enter the event settings. 6. Tap new to specify the time. 7. -
Page 25: Deleting Program Lines
Operating the Detector Deleting Program Lines Program lines can be deleted while creating a program. 1. Tap Tab in the program's editing window to display the program lines. 2. Tap to mark the desired program line. 3. Tap Del to delete the desired program line. 4. -
Page 26: Displaying The Program Lines
Operating the Detector 5. Tap to go to a higher level, or hold it two seconds to go to the Main menu. Edit: Program 2 Line: 1 Channel 2: Channel 1: λ: λ: Event 0.0 min Fig. 17 Program 2 > Edit Displaying the Program Lines 1. -
Page 27: Link Menu
Operating the Detector Link Menu Links contain connections between existing programs, which can be defined and edited, like the programs themselves. A maximum of 10 links between defined programs can be created and saved. Navigating through 1. Tap the Link menu to display the Link list. the Link menu 2. -
Page 28: Executing A Link
Operating the Detector Link List Edit Edit: Link Line: 1 Program: Repeat: for external signal Wait: Line: Select: Wait status no wait for external signal Fig. 20 Creating a link Executing a Link 1. Tap the Link menu to display the Link list. 2. -
Page 29: Scan Menu
Operating the Detector Scan Menu Navigating the 1. Tap on the Scan menu in the Main menu to display options. Scan menu 2. Tap the gray highlighted values in the range line to determine the wave- length range for autozero and scan. 3. -
Page 30: Setup Menu Parameters
Operating the Detector 6. Tap to go to a higher level, or hold it two seconds to go to the Main menu. Menu > Setup Scan Network Event Lamps Analog Date Analog T-const. Intensity Fraction Fig. 23 Setup menu Setup Menu Parameters Network The network configuration is displayed: Setup:... - Page 31 Operating the Detector Intensity The intensities in channel C1 are displayed. Monitor source can be selected (absorption, signal channel, reference channel). Setup: intensities - channel 1 I-sig: I-sig: 0.0562 511 nW I-ref: 0.0275 I-ref: 220 nW Integr.: 756 nm absorption Monitor source: Fig.
- Page 32 Operating the Detector By selecting Set to zero an applied voltage can be defined as the spectral zero point for the wavelength 000 nm. For this purpose a voltage of 0 V will be normally used. Setup: Analog in Actual interpreted wavelength: 000 nm Procedure: Set to Zero Fig.
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Page 33: Glp Menu
Operating the Detector Event By tapping the gray field in the active only line, the condition of the event connections can be checked. Setup: Event check Start-IN: Error-IN: AutoZero-IN: Active only: Event 3 Fig. 32 Setup > Event Date Here the date and time for the detector can be entered, after tapping the gray buttons next to Date and Time. -
Page 34: Functionality Tests
Installation Qualification (IQ) The customer may request the Installation Qualification, which is free of charge. In case of a request, the Technical Support of KNAUER or from a provider authorized by KNAUER performs this functionality test during the installation. The Installation Qualification is a standardized document that comes as part of the delivery and includes the following: ... -
Page 35: Possible Problems And Rectifications
Troubleshooting Possible Problems and Rectifications Problem Solution Baseline drift Maintain constant temperature condi- tions during the measurement. Device will not turn on Inspect the power cable to be sure it is plugged into the power supply. Device cannot be cali- 1. -
Page 36: System Messages
System Messages Can the devices communicate with the computer, even though the router is disconnected from the company network? 5. In case you own a Control Unit, check the settings in the menu Setup > Network. Is LAN-DHCP set for controlling? ... - Page 37 System Messages System message Solution Filter move error Switch the device off and on. If the system message appears again, notify Technical Support. This entry is not executable. Instrument remote con- trolled Quit software. Check the cable connections. Invalid command Change the entry.
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Page 38: Maintenance And Care
If you have any technical questions regarding the hardware or software of Technical Support the manufacturer, please use one of the contact options below: European hotline Languages: German and English Available by telephone: 8 am to 5 pm (CET) Phone:+49–(0)30–809727–111 Fax:+49–(0)30–8015010 E-mail: support@knauer.net 50D user manual V7654, version 2.0... -
Page 39: Maintenance Contract
Maintenance and Care Maintenance Contract The following maintenance work on the device may only be performed by the manufacturer or a company authorized by the manufacturer and is cov- ered by a separate maintenance contract: Opening the device Removing the hood or the side panels. -
Page 40: Cleaning The Lenses Of An Analytical Flow Cell
Maintenance and Care Cleaning the Lenses of an Analytical Flow Cell Legend Seal ring Lens Compression part Threaded ring Fig. 35 Analytical flow cell Prerequisite Device has been switched off. Power plug has been pulled. Flow cell has been removed. ... -
Page 41: Cleaning The Fiber Optics Of A Preparative Flow Cell
Maintenance and Care Cleaning the Fiber Optics of a Preparative Flow Cell The preparative flow cells have a rod shaped light guide instead of the con- cave lens of the analytical cells. Legend Threaded ring Cover Spacer Compression bushing Light guide with seal ring Seal Fig. -
Page 42: Storage
Storage Legend Inlet of the flow cell Outlet of the flow cell Knurled-head screws Cover plate Carriage Fig. 37 Replacing the flow cell Procedure Proceed as follows: 1. Switch off the device and pull the power plug. 2. Remove capillaries at the inlet and outlet of the flow cell. -
Page 43: Legal Information
Legal Information Noise ± 0.75 x 10 AU at 254 nm (ASTM E1657- Drift 1.5 x 10 AU/h at 254 nm (ASTM E1657-98) Linearity > 2.2 AU at 270 nm (ASTM E1657-98) Time constants 0.1/ 0.2/ 0.5/ 1.0/ 2.0/ 5.0/ 10.0 s Integration time Automatic Communication... -
Page 44: Warranty Conditions
2001), old devices manufactured by KNAUER are marked as waste electrical and electronic equipment: 160214. WEEE Registration KNAUER as a company is registered by the WEEE number DE 34642789 in the German "Elektroaltgeräteregister" (EAR). The number belongs to cate- gory 8 and 9, which, among others, comprise laboratory equipment. -
Page 45: Abbreviations And Terminology
Abbreviations and Terminology Abbreviations and Terminology Here you can find information on the abbreviations and terminology used in this device manual for the detector. Terminology Meaning Degasser Degasser module for fluids, e. g., in a high- pressure pump. Good Laboratory Practice – quality assur- ance for laboratories. -
Page 46: Index
Index Index Abbreviations GLP menu Additives Graphical user interface structure Ambient conditions Analog in HPLC Analog out Analog port Example Installation location, see location Autozero Scan Integrator Connector AVV marking Integrator outlets Intensity Buttons Touchscreen Laboratory use Lamps Capillaries Capillary port Clamping ring problems... - Page 47 Index Storage Switching the detector on Rear view of the device Symbols Replacing the flow cell System Messages router (LAN) T-const. Safety Technical Data safety equipment Technical data Salts Technical Support Scan Terminology Scan menu test Scope of delivery Installation Qualification Setup menu Operation Qualification Signal options...
- Page 48 KNAUER Wissenschaftliche Geräte GmbH © All rights reserved. Technical data are subject to change without notice. Please check our website for latest updates and changes. Translation of the original German edition of this manual, version 2.0 Last manual update: 2016-04-20 Printed in Germany on environmentally friendly paper from sustainable forests.
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