Table of Contents
Advertisement
Quick Links
Advertisement
Table of Contents
Subscribe to Our Youtube Channel
Related Manuals for Knauer Azura UVD 2.1L
Summary of Contents for Knauer Azura UVD 2.1L
- Page 1 Detector UVD 2.1L Instructions HPLC Document No. V6831...
- Page 2 For the printed versions of our instructions, we use environmentally friendly paper from sustainable forests. Copyright: This document contains confidential information and may not be repro- duced without written consent of KNAUER Wissenschaftliche Geräte GmbH. © KNAUER Wissenschaftliche Geräte GmbH 2019 All rights reserved.
-
Page 3: Table Of Contents
Table of contents Table of contents 1. General ................1 About these instructions . - Page 4 Table of contents Power supply............12 Connecting the leak management .
- Page 5 Table of contents Cleaning the flow cell........... . 38 8.4.1 Rinsing the flow cell .
-
Page 6: General
These operating instructions are an integral part of the device. It must be kept in the immediate vicinity of the device and accessible to the user at all times. You can download these and other instructions from the KNAUER web- site: www.knauer.net/library... -
Page 7: Legal Information
1.4.4 Warranty seal A blue or orange warranty seal is affixed to some devices. A blue seal is used by KNAUER’s Manufacturing or Customer Support ƒ for devices to be sold. After repair, service technicians attach an orange seal onto the identi- ƒ... -
Page 8: Basic Safety Instructions
Operation in potentially explosive areas without special and additio- ƒ nal explosion protection. Contact the KNAUER Customer Support for more information. User qualification The user is qualified to handle the device if all of the following points apply: He has at least a basic knowledge of liquid chromatography. -
Page 9: Operator Responsibility
Basic safety instructions Operator responsibility The operator is any person who operates the device himself or leaves it to a third party for use and who bears the legal product responsibility for the protection of the user or third parties during operation. The obligations of the operator are listed below: Know and follow the applicable work safety regulations ƒ... -
Page 10: Working With Solvents
Basic safety instructions Working with solvents 2.6.1 General requirements Qualification: The user is trained for handling different solvents. ƒ Chemical compatibility of wetted materials: Note recommended sol- ƒ vents and concentrations in these instructions in order to avoid perso- nal injury or damage to the device (see „13 Chemical compatibility of wetted materials“, p. -
Page 11: Specific Environments
Devices which are shipped without the completed document “Service request form and decontamination report” will not be repaired. If you would like to return a device to KNAUER, make sure to enclose the com- pleted document: www.knauer.net/servicerequest AZURA® Detector UVD 2.1L Instructions, V6831... -
Page 12: Product Information
3. Product information Features 3.1.1 Detector The UV/VIS detector AZURA® UVD 2.1L is a variable single wavelength detector, designed for measuring quickly and precisely in the ultraviolet and visual spectral range. The detector can be used in analytical and preparative HPLC systems. It is used in laboratories to analyze substance mixtures. -
Page 13: Views
Product information Views Legend Leak tray Capillary guides Standby switch Status LEDs Flow cell Fig. 1: Front view Legend Serial number label Integrator output LAN port RS-232 port (service only) Pin header Power connection Power switch Fig. 2: Rear view AZURA®... -
Page 14: Meaning Of The Leds
Product information Meaning of the LEDs There are three LEDs and a standby switch on the front of the device. The figure shows the LED panel when the device is switched off. The LEDs show different colors depending on the operating status. Color Operating status Measures... -
Page 15: Symbols And Signs
Product information Symbols and signs The following symbols and signs can be found on the device: Symbol Meaning High-voltage hazard Electrostatic discharge hazard, damages to sys- tem, device, or components can occur. Obey maximum load for leak tray during transpor- tation, installation and operation. -
Page 16: Installation And Initial Startup
4. Installation and initial startup Before you determine the operation site, read chapter 11 on page 43. There you will find all device-specific information on power supply, am- bient conditions and humidity. Note: The intended use be ensured only if the requirements for ambient conditions of the operating environment are met. -
Page 17: Space Requirements
Country-specific plugs: Before switching on the device, check whether ƒ the supplied plug is approved for your country. Overview of the de- vice- and country-specific plug types from KNAUER: www.knauer.net/plugs Power strips: If several devices are connected to one power strip, al- ƒ... -
Page 18: Connecting The Leak Management
Installation and initial startup Connecting the leak management The leak management consists of the leak sensor and the drainage sys- tem. The drainage system ensures that escaping liquids flow into a waste bottle. If there is too much liquid, the red LED starts flashing. Both the de- vice and the data acquisition via chromatography software are stopped. -
Page 19: Inserting The Flow Cell
Installation and initial startup Inserting the flow cell Note: The test cell is used during operation qualifications and must be stored. Prerequisite Detector is switched off. ƒ Power plug is disconnected from the device. ƒ Steps Figure 1. Unscrew the 2 knur- Procedure led-head screws 2. -
Page 20: Setting The Optical Path Length Of A Preparative Flow Cell
Installation and initial startup Setting the optical path length of a prepa- rative flow cell Depending on type, the path length is factory set to 2 mm, 3 mm, or 10 mm. For the 2 mm flow cells you can remove the spacers on one or both sides and hence change the path length to 1.25 mm or 0.5 mm. -
Page 21: Connecting Capillaries
Installation and initial startup Connecting capillaries Component defect Damage to components due to excessive tightening possible. Observe the torque of the screw connection Use 5 Nm torque for stainless steel fi ttings. Use 1 Nm torque for PEEK fi ttings. Note: PEEK fittings withstand a maximum pressure of 400 bar. Prerequisite Flow cell is removed from the detector. -
Page 22: Computer Control
Installation and initial startup Computer control Note: HPLC devices from KNAUER only work with IP addresses, which have been assigned by IPv4. IPv6 is not supported. This chapter describes how to set up a chromatography system in a local area network (LAN) and how a network administrator can integrate this LAN into your company network. -
Page 23: Connecting Devices With Lan
Set the router properties (see section 4.8.4). 4.8.4 Configuring the router The router is preset at the factory. Information about address, user name and password is noted in the router manual: www.knauer.net/router Process Procedure 1. To open the router configuration, start your Internet browser and enter the IP address (not for all routers). -
Page 24: Integrating Lan Into Company Network
Installation and initial startup Result Once the router has assigned IP addresses to all devices, the chromato- graphy software can be used to remotely control the system. 4.8.5 Integrating LAN into company network A network administrator can integrate the LAN into your company net- work. -
Page 25: Setting Ip Addresses
Installation and initial startup Setting IP addresses Note: Set a static IP address. Note: Check the IT security standards for your lab before intervening in the LAN settings. There are two ways for the device to set a fixed (static) or dynamic (DHCP) IP address via software: Mobile Control or Firmware Wizard. -
Page 26: Mobile Control: Setting A Dynamic Ip Address Via Device Name
Installation and initial startup 4.9.2 Mobile Control: Setting a dynamic IP address via device name Prerequisites The device is switched on. ƒ Mobile Control is installed and running. ƒ The connection between Mobile Control and the device has been ƒ established. -
Page 27: Firmware Wizard: Setting A Static Ip Address
Installation and initial startup Abb. 15: Reset device to default settings 4.9.4 Firmware Wizard: Setting a static IP address Note: More information about LAN settings can be found in the Mobile Control Software Instructions in the chapter "Firmware Wizard” (docu- ment no. -
Page 28: Firmware Wizard: Setting A Dynamic Ip Address
Installation and initial startup Process 4. Enter the IP address into the text field <IP address> 5. Optionally, adjust subnet mask and gateway 6. Click <Reset Conn. Settings> to accept changes. 7. Restart the device (recommended). Result The device is now accessible via the static IP address. 4.9.5 Firmware Wizard: Setting a dynamic IP address Prerequisites The device is switched on. -
Page 29: Remote Control
Installation and initial startup 4.10 Remote control For receiving start, control, and error signals from external devices ƒ For sending start, control and error signals to external devices ƒ Fig. 17: Relation between display and Events terminal strip Connector assignment 4.10.1 Plug Function... - Page 30 Installation and initial startup Plug Function Error IN TTL input Low active ƒ Secure switching threshold at least 10 mA ƒ After receiving a signal (short circuit to ground) ƒ from an external device, an error message appears and the device stops. TTL Input Start IN Low active...
-
Page 31: Analog Control
Installation and initial startup 4.10.2 Analog control Using the analog port, you can control the wavelength by changing the applied voltage. A Mobile Control is required in order to select the option ANALOG in the SETUP menu. To use the analog port for controlling the detector, you have to set a zero Example point and enter a scaling value. -
Page 32: Switching On
Installation and initial startup Fig. 18: Operating tool Process 1. Insert the operating tool in an upper small opening at the front of the pin header 2. Insert the cable into the opening underneath the inserted opera- ting tool. 3. Pull out the operating tool. Check whether the cables are tightly attached. -
Page 33: Operation
The network connection to the router is established ƒ (see „4.8.4 Configuring the router“, p. 18). A chromatography software has been installed by KNAUER or a com- ƒ pany authorized by KNAUER. Capillaries from the column to the UV detector and capillaries from the ƒ... -
Page 34: Setting The Wavelength
Operation Setting the wavelength You can set a wavelength between 190–750 nm. Resetting the device Using the Mobile Control, you can re-set the detector to its default settings. Parameter Settings Network LAN DHCP, port 10001 Lamps D2 ON Time constant Channels Channel 1: WL = 254 nm Analog out... -
Page 35: Functionality Tests
Installation Qualification (IQ) The customer may request the Installation Qualification, which is free of charge. In case of a request, the Technical Support of KNAUER or from a provider authorized by KNAUER performs this functionality test during the installation. -
Page 36: Troubleshooting
7. Troubleshooting First measures: Check all cables and fittings. ƒ Check if air has gotten into the supply lines. ƒ Check the device for leakages. ƒ Check system messages in the software (chapter (see chapter 7.3 on ƒ page 33)). Further measures: Compare occurring errors with the list of possible errors (see below). -
Page 37: Possible Problems And Solutions
Troubleshooting Possible problems and solutions Problem Solution Baseline drift Maintain constant temperature conditions during the measurement. Device will not turn on Inspect the power cable to ensure that it is plugged into the power supply. Device cannot be 1. Fasten the knurled-head screws on the calibrated slide to prevent incursion from interfe- ring light or an electronics error. -
Page 38: Error Messages
Troubleshooting Error messages If other error messages are displayed besides those listed below, please restart the device. Inform the Customer Support of the manufacturer in case the error mes- sage displays repeatedly. Message Measure Calibration failed 1. Restart the device. 2. - Page 39 Troubleshooting Message Measure Cannot proceed: lamps 1. Switch the lamp on. If the error occurs are off. again, restart the device. 2. If the lamp is off after restarting, repla- ce the lamp. Cannot read data from Restart the device. FRAM Cannot read RTC Restart the device.
- Page 40 Troubleshooting Message Measure Link is running 1. Wait until the link has been completed. 2. Change the link or delete it. Motor end position rea- The motor that controls the wavelength ched Instrument will be reached its end position and stopped. recalibrated Recalibrate the device.
-
Page 41: Maintenance And Care
Replace the flow cell. ƒ 8.1.1 Maintenance contract The device may only be opened by the Technical Service of KNAUER or any company authorized by KNAUER. These maintenance tasks are part of a separate maintenance contract. AZURA® Detector UVD 2.1L Instructions, V6831... -
Page 42: Cleaning And Caring For The Device
Maintenance and care Cleaning and caring for the device Device defect Intruding liquids can cause damage to the device. Place solvent bottles next to the device or in a solvent tray. Moisten the cleaning cloth only slightly. All smooth surfaces of the device can be cleaned with a mild, commerci- ally available cleaning solution, or with isopropanol. -
Page 43: Cleaning The Flow Cell
Maintenance and care Cleaning the flow cell Increased baseline noise and reduced sensitivity can be a result of a dirty flow cell. Often it is sufficient to rinse the flow cell to restore optimal sensitivity. Note: Dirty lenses or fiber optic connectors could falsify the measure- ment. -
Page 44: Cleaning The Lens Of An Analytical Flow Cell
Maintenance and care 8.4.2 Cleaning the lens of an analytical flow cell Prerequisite Flow cell is removed from the detector. Tools Tweezers ƒ Allen screwdriver, size 3 ƒ Threaded ring Compression part Lens Seal ring Fig. 22: Analytical flow cell Procedure Process 1. -
Page 45: Cleaning The Light Guide Of A Preparative Flow Cell
Maintenance and care 8.4.3 Cleaning the light guide of a preparative flow cell The preparative flow cells have a rod shaped light guide instead of the concave lens of the analytical cells. Prerequisite Flow cell is removed from the detector. Tools Tweezers ƒ... -
Page 46: Replacing The Fiber Optics
Maintenance and care Next steps Mount the flow cell onto the detector. Replacing the fiber optics UV light will cause the fiber optics to become blind with time (solariza- tion), making them no longer suitable for use. Note: Observe the following regarding the use of fiber optics: Do not touch the ends of the fiber optics with your fingers, as this ƒ... -
Page 47: Transport And Storage
Do not hold onto front cover or leak tray, as these parts are loosely attached to the device. Transporting the device Documents: If you want to return your device to KNAUER for repairs, ƒ enclose the Service Request Form and Decontamination Report which can be downloaded from our website:www.knauer.net/servicerequest... -
Page 48: Disposal
160214. 10.2 WEEE registration number KNAUER as a company is registered by the WEEE number DE 34642789 in the German "Elektroaltgeräteregister" (EAR). The number classifies to category 8 and 9, which, among others, comprises laboratory equipment. -
Page 49: Technical Data
11. Technical data 11.1 Detection Detector type Variable single wavelength UV/VIS detector ƒ Variable single wavelength UV/VIS detector ƒ with fiber optic connectors Detection channels Light source Deuterium (D ) lamp with integrated GLP chip Wavelength range 190–750 nm Spectral bandwidth 11 nm at H line (FWHM) α... -
Page 50: General
If you have any questions regarding spare parts or accessories, please contact our Customer Support. Current information on spare parts and accessories can be found on the Further information Internet at www.knauer.net. 12.1 Devices Name Order no. AZURA® UV/VIS Detector UVD 2.1L without flow cell ADA01XA AZURA®... -
Page 51: Software
Reorders 12.2 Software Name Order no. Mobile Control license A9610 Mobile Control Chrom license A9612 Mobile Control license with 10“ touchscreen A9607 Mobile Control Chrom license with 10“ touchscreen A9608 12.3 Accessories and spare parts Name Order no. Tool kit AZURA® A1033 Repair kit for analytical flow cells A1131... -
Page 52: Preparative Flow Cells
Reorders Technical data Order No. Path length 3 mm A4042 Connection 1/16“ Fiber optics version: Inner diameter 1.0 mm A4044 Volume 2 μl High temperature Material Stainless steel fiber optics version: Max. flow rate 50 ml/min A4044HT Max. pressure 300 bar Max. temperature 85°C (A4044HT only) Path length 3 mm... -
Page 53: Preparative Flow Cells - Fiber Optics
Reorders Technical data Order No. Path length 0.5 mm A4095 Connection 1/16“ Fiber optics version: Inner diameter 0.8 mm A4096 Volume 3 μl Material PEEK Max. flow rate 250 ml/min Max. pressure 100 bar 12.5.3 Preparative flow cells - fiber optics Technical data Order No. -
Page 54: Chemical Compatibility Of Wetted Materials
The following list contains information about the chemical compatibility of all wetted materials which are used in devices made by KNAUER. The data bases on a literature research on the manufacturer specifications of the materials. The wetted materials of this device are listed in the chapter “Technical data”. - Page 55 Chemical compatibility of wetted materials Polyimide (Vespel®) This material is wear-resistant and permanent resilient thermically (up to 200 °C) as well as mechanically. It is chemically broadly inert (pH range 1-10) and is especially resistant against acidic to neutral and organic solvents, but vulnerable to pH strong chemical or oxidizing environments: It is incompatible with concentrated mineral acids (such as sulfuric acid), glacial acetic acid, DMSO and THF.
-
Page 56: Non-Metals
Chemical compatibility of wetted materials is moderately resistent against ether, halogenated solvents and toluene. Halogenated solvents over +60 °C and chlorine gas should not be used. Fluorinated rubber (FKM) The elastomer consisting of fluorinated hydrocarbon stands out due to a high resistance against mineral oils, synthetic hydraulic fluids, fuels, aromatics, and many organic solvents and chemicals. -
Page 57: Metals
Chemical compatibility of wetted materials Glass, glass fibre, quartz, quartz glass These mineral materials are resistant against corrosion and wear and are mostly chemical inert. They are compatible with oils, fats and solvents and show a high resistance against acids anf lyes up to pH values of 3-9. Con- centrated acids (especially hydrofluoric acid) may embrittle and corrode the minerals. -
Page 58: Index
14. Index Ambient conditions 11, 42 Initial startup 11 Air humidity 45 Inputs 44 Analog control 26 Installation 11 Analog input 45 Installation Qualification (IQ) 30 Analog output 45 Integration time 44 Analytical flow cell 39 Integrator connector 26 AVV-marking 43 Intended use 3 Interfaces 44 IP address... - Page 59 Index Product information 7 Remote control 24 Reorders 45 Resetting 29 Router 18 Safety features 4 instructions 3 Personal safety equipment 4 Scope of delivery 7 Service request form 6, 42 Signal words 1 Software control 28 solvents 5 Solvents chemical compatibility 49 disposal 43 health-threatening 5...
- Page 60 Latest KNAUER instructions online: www.knauer.net/library KNAUER Phone: +49 30 809727-0 Wissenschaftliche Geräte GmbH Fax: +49 30 8015010 Hegauer Weg 38 E-Mail: info@knauer.net 14163 Berlin Internet: www.knauer.net © KNAUER 2019...
Need help?
Do you have a question about the Azura UVD 2.1L and is the answer not in the manual?
Questions and answers