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Related Manuals for Knauer Azura RID 2.1L HighFlow
Summary of Contents for Knauer Azura RID 2.1L HighFlow
- Page 1 Detector RID 2.1L / RID 2.1L HighFlow Instructions HPLC HPLC Document no. V6750...
- Page 2 Keep the instructions for future reference. Note: In case you require a version of this instruction in another lan- guage, please submit your request including the corresponding article number via e-mail or fax to KNAUER. Technical Support: Phone: +49 30 809727-111 (9-17h, Central European Time)
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Page 3: Table Of Contents
Table of contents Table of contents Product information............1 Intended use. - Page 4 Table of contents 7.2.5 LED power factor ..........29 7.2.6 Extended Dynamic Range .
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Page 5: Product Information
Product information 1. Product information The AZURA® Detector RID 2.1L / RID 2.1L HighFlow is a sensitive and competitively priced differential refractometer which is suitable for detecting compounds with little or no UV activity such as alcohols, sugars, lipids or polymers in high concentrations. The device is a member of the AZURA L product line and shares a num- AZURA®... -
Page 6: Operating Ranges
Product information Operating ranges The AZURA® Detektor RID 2.1L can be used in the following areas for analytical applications (with flow rates up to 10 ml/min): ƒ Food analysis ƒ Chemical analysis ƒ Pharmaceutical analysis ƒ Environmental analyses ƒ Biochemical analysis The AZURA®... -
Page 7: Performance Features
GLP data, such as the operating hours of the device or light sources. For a detailed description of viewing or reading the GLP data, see the appro- priate instructions for the software products: (https://www.knauer.net/en/Support/User-manuals/Software). Light intensity and balance, zero glass position and LED current can be Troubleshooting easily monitored via the Mobile Control and supported software products to ensure optimal working conditions for your analysis. -
Page 8: Functional Principle
Product information AZURA® Neo The AZURA® Neo electronic platform features: ƒ New microprocessor for faster device performance ƒ Communication interfaces: IP dual stack with switch (for connecting AZURA® devices among each other) and LAN stack function plus USB service interface (internal USB to RS-232). Both LAN ports (1 and 2) can be used as interface or as switch. -
Page 9: Calculating The Signal Value
Product information different refractive index, the light beam is geometrically proportionally deflected depending on the relative change of the refractive index (according to Snell´s law). This results in a change of the light intensities I and I (one increases and the other decreases), proportional to concentration and refractive index of the sample solution. - Page 10 Product information Legend Solvent Heat exchanger Sample cell Reference cell Flush valve Temperature con- trolled compart- ment Waste Flushing process When the flush valve is activated the solvent takes the following flow path (see figure below): 1. Enters through IN port. 2.
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Page 11: Scope Of Delivery
Product information 2. Scope of delivery Note: Only use spare parts and accessories made by KNAUER or a com- pany authorized by KNAUER. ƒ Power supply cable ƒ AZURA® Detector RID 2.1L / RID 2.1L HighFlow device ƒ AZURA® Detector RID 2.1L / RID 2.1L HighFlow accessory kit ƒ... -
Page 12: What Must The User Take Into Account
(wall socket or power strip). Opening the device The device may be opened by the KNAUER Technical Support or any company authorized by KNAUER only. AZURA® Detector RID 2.1L / RID 2.1L HighFlow Instructions V6750... -
Page 13: Warning Notifications
Decontamination Report Devices without a completed Decontamination Report will not be repai- red. If you would like to return a device to KNAUER, make sure to enclose a completed Decontamination Report with the device: https://www.knauer.net/decontamination-report. -
Page 14: Symbols And Signs
Product information 4. Symbols and signs The following symbols and signs can be found on the device: Symbols Meaning High-voltage hazard Electrostatic discharge hazard, damages to sys- tem, device, or components can occur. Obey maximum load for leak tray during transpor- tation, installation and operation. -
Page 15: Unpacking The Detector
Product information Device defect The device overheats at exposure to sunlight and insufficient air circula- tion. Device failures are very likely. Set up the device in such a way that it is protected against exposure to direct sunlight. Leave room for air circulation: See paragraph „space requirements“. General requirements ƒ... -
Page 16: Initial Startup
Electronic defect Electronic hazard when using an identically constructed power adapter from another manufacturer. Only use spare parts and accessories from KNAUER or a company authorized by KNAUER. Note: Make sure that the power adapter and power cables meet the requirements (see 11.3 on page 41). - Page 17 Product information Procedure Process Figure 1. Carefully push the funnel into the center opening of the capillary guide Fig. 4: Funnel and capillary guide 2. Push the long ending of the first nozzle into the hose Fig. 5: Hose and nozzle 3.
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Page 18: Connecting The Capillaries
Product information Connecting the capillaries Component defect Damage to the flow cell due to excessive tightening. Observe the torque of the screw connection. Use 5 Nm for stainless steel fi ttings Use 0.5 Nm for PEEK fi ttings. Before connecting capillaries, note the following issues: Important information for ƒ... -
Page 19: Azura® Detector Rid 2.1L Highflow
Product information Procedure Figure 3. Insert the capillary as far as possible into the IN port OUT port at the front of the device. 4. Tighten the fitting finger tight. Check if all fittings are tight. Next steps 6.2.2 AZURA® Detector RID 2.1L HighFlow At the front side, both connections are designed for 1/4'' UNF flat bottom fittings. -
Page 20: Integrating The Detector Into The System
Product information OUT port Procedure Figure 1. Push the capillary through the fitting 2. Slide the lock ring over the tubing. Note that the tapered end of the lock ring (with the thin edge) has to point to the seal ring 3. -
Page 21: Connecting The The Detector To The Computer Via Lan
You find a detailed description on chromatography software in a corre- sponding instruction. Note: HPLC devices made by KNAUER work only with IP addresses which are assigned via IPv4. IPv6 is not supported. IPv6 is not supported. This chapter describes how to set up a chromatography system in a local area network (LAN) and how a network administrator can integrate this LAN into your company network. -
Page 22: Configuring The Lan Settings
Product information Process 1. On the computer, go to the control panel and check the LAN properties. 2. Hook up the router to the devices and the computer. 3. On the computer, configure the router to set up the network. 4. -
Page 23: Configuring The Router
Product information Prerequisites ƒ The computer has been switched off. ƒ There is a patch cable for each device and the computer. Procedure 1. Use the patch cable to connect the router and the computer. Repeat this step to connect all devices. 2. - Page 24 Product information Note: Set a fixed IP address. Note: Check the IT safety standards for your lab before changing the LAN settings. Two options are given to set the device IP address to fixed (static) or dynamic (DHCP) via software: Mobile Control or Firmware Wizard. Mobile Control oder Firmware Wizard.
- Page 25 Product information Mobile Control: Setting IP address to DHCP via device serial number Prerequisites ƒ The device has been switched on. ƒ Mobile Control has been installed and started. ƒ The connection between the Mobile Control and the device has been established.
- Page 26 Product information Firmware Wizard: Setting dynamic IP address Prerequisites ƒ The device has been switched on. ƒ Firmware Wizard has been installed and started. ƒ The connection between Firmware Wizard and the device has been established. 1. In Firmware Wizard, click <Reset LAN Settings...>. Procedure 2.
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Page 27: Remote Control
Product information Remote control On the rear of the valve drive are sockets on a terminal strip. Signals can be send and received by other devices via those sockets. The signals are for example start signals of a injection valve or autosampler which is connected to the START connector. -
Page 28: Connecting Cables To The Pin Header
Product information Plug Function START OUT TTL output (default setting)/ OC with external pull-up to 24 V (25 mA) Levels: ƒ passive 5 V ƒ active 0 V START IN TTL-compatible input ƒ Low-active Secure switching threshold at least 10 mA After receiving a signal (short-circuit to ground) from an external device, the detector starts. -
Page 29: Integrator Port
Product information Electronic defect Connecting cables to the multi-pin connector of a switched on device cau ses a short circuit. Turn off the device before connecting cables. Pull the power plug. Electronic defect Electrostatic discharge can destroy the electronics. Wear a protective bracelet against electrostatic discharge and ground. Process 1. -
Page 30: Operation
Product information 7. Operation The detector can be operated in two ways: ƒ Control with chromatography software ƒ Operating with Mobile Control Note: It is not possible to use two control methods simultaneously. If the detector is connected to the software, it cannot be controlled via Mobile Control. -
Page 31: Default Settings
Product information Color Operating condition Operation flashes green equilibrated Wait until the device is ready. green Ready for operation Right LED green Switched on (Operational status) blue standby Exit with the standby switch. Standby To start the standby, keep the switch pressed for 5 seconds. Note: Malfunctioning system after repeated standby possible. -
Page 32: Autozero
Product information 7.2.1 Autozero The autozero command leads to the zeroing of the detector signal. This command can be carried out via software or via analog command. This is a two leveled function: ƒ Level one is an electronic or digital zeroing of the signal. ƒ... -
Page 33: Led Power Factor
Product information It is recommended to set the time constant and data rate in relation to peak width. Peak width [min] Time constant [s] Data rate [Hz] < 0.003 0.01 > 0.007 0.02 > 0.017 0.05 > 0.033 >0.067 >0.167 >... -
Page 34: Extended Dynamic Range
Product information LED durability By decreasing the LED power factor, the lifetime of the LED light source can be increased. 7.2.6 Extended Dynamic Range The option Extended Dynamic Range enables the extension of the dynamic measuring range to +100 % (–1000 μRIU offset for AZURA® Detector RID 2.1L and –2500 μRIU offset for AZURA®... -
Page 35: Glp
Product information The following GLP data of the detector can be found in the software. GLP data Unit Explanation Device information Serial number FRAYYWWXXXXX Firmware version Current firmware version of device Operating time Operating hours after production Date of installation Date of manufacture Last maintenance Light source... -
Page 36: Flushing The Flow Cell
Product information Device defect Intruding liquids can cause damage to the device. Place solvent bottles next to the device or in a solvent tray. Moisten the cleaning cloth only slightly. Note: State of safe operation is achieved even after the power connec- tion has been interrupted because of power cut or emergency shutdown. -
Page 37: Activate Standby
Product information Procedure 1. Start the flush mode. 2. Check if the LED blinks green. 3. After a period of about 30 seconds, stop the flush mode. 4. Wait for the end of program. Result The detector is now ready to operate. Next Steps Start the measuring. -
Page 38: Functionality Tests
The customer may request the Installation Qualification, which is free of Installation Qualification charge. In case of a request, the Technical Support of KNAUER or from a (IQ) provider authorized by KNAUER performs this functionality test during the installation. -
Page 39: Troubleshooting
Product information 9. Troubleshooting First measures: ƒ Check all cables. ƒ Check all fittings. ƒ Check if air has gotten into the supply lines. ƒ Check the detector for leaks. Error analysis In your software, a number of diagnostic parameters are displayed for monitoring the state of your device. -
Page 40: Device Problems
Further Measures ƒ Install the maintenance software (Service Tool). ƒ Save device information and send it to KNAUER. ƒ Inform technical support. System messages If other system messages are displayed besides those listed below, please turn the detector off and then on. - Page 41 Product information Message Cause Solution “Instrument in standby Command not allowed during Quit standby. mode” standby mode. “Leak sensor failed” Leak sensor sensor was not found Restart detector. or does not react. If the leak sensor cannot be found again, contact the Technical Support.
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Page 42: Proceeding With Tasks After A Leak
Maintenance contract The detector may only be opened by the Technical Support of KNAUER or any company authorized by KNAUER. This maintenance work is cov- ered by a separate maintenance contract. -
Page 43: Check Calibration/Validation
Product information ƒ Regularly check if all fittings are tight (5 Nm for stainless steel fittings, 0.5 Nm for PEEK fittings). ƒ All smooth surfaces of the device can be cleaned with a mild, commer- cially available cleaning solution, or with isopropanol. Device defect Intruding liquids can cause damage to the device. -
Page 44: Technical Data
Product information Life-threatening injuries Health danger if getting in contact with toxic, infectious or radio-active substances. Before disposing of the device or sending it away for repair, you are required to decontaminate the device in a technically correct manner. Procedure Figure 1. -
Page 45: Communication
THF, but also when operating a HPLC system in recycling mode. Therefore, when working with THF and a detector, KNAUER recommends the use of stabilized THF and a THF resistant degasser. In addition, we also recommend to avoid the delivery of THF in a cycle (e. -
Page 46: Plastics
The following list contains information about the chemical compatibility of all wetted materials which are used in devices made by KNAUER. The data bases on a literature research on the manufacturer specifications of the materials. The wetted materials of this device are listed in the chapter "Technical data". - Page 47 Product information Perfluorethylenpropylen-Copolymer (FEP), Perfluoralkoxy-Polymer (PFA) These fluorinated polymers hold similar features as PTFE, but with a lower operation temperaturte (up to 205 °C). PTA is suitable for ultrapure appil- cations, FEP can be used universally. They are resistant against almost all organic and inorganic chemicals, except elemental fluorine under pres- sure or at high temperatures and fluorine-halogen compounds.
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Page 48: Non-Metals
Product information 12.3 Non-metals Diamond-like carbon (DLC) This material is characterized by a high hardness, a low coefficient of fric- tion and thus low wear. In addition, it is highly biocompatible. DLC is inert against all acids, alkalis and solvents commonly used in HPLC. Ceramic Ceramic is resistant against corrosion and wear and is fully biocompati- ble. -
Page 49: Repeat Orders
Product information They are inert against almost all solvents. Exceptions are biological appli- cations which are metal ion sensible, and applications with extreme cor- rosive conditions. These steels, in comparison to commonly used steels, are increasingly resistant against hydrochloric acid, cyanides and other halogen acids, chlorides and chlorinated solvents. -
Page 50: Legal Information
A warranty seal is attached on some devices. The warranty seal is col- orcoded. A blue seal is used by the assembly or technical support of KNAUER for devices to be sold. After repair, service technicians stick an orange seal in identical position. If unauthorized persons interfere with device or the seal is damaged, the warranty claim becomes void. -
Page 51: Declaration Of Conformity
160214. 160214. 14.5.2 WEEE registration KNAUER as a company is registered by the WEEE number DE 34642789 in the German "Elektroaltgeräteregister" (EAR). The number belongs to category 8 and 9, which, among others, comprises laboratory equipment. - Page 52 HPLC glossary Term Definition Degasser Degasser module for fluids Detector device measuring the composition or the quantity of a substance. Good laboratory practice Good Laboratory Practice) Gradient Time-dependent composition of solvent (mobile phase) on low-pres- sure or high-pressure side of system Isocratic Mode of sample separation where the composition of a solvent remains constant...
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Page 53: Index
Index 16. Index absorption 47 Firmware Wizard 21 accessories 45 flow cell adsorption 47 flushing 32 ambient conditions 41 type 40 Analog outputs 41 flow path 5 analog output scaling 30 flow rate 40 analytical 47 functionality tests 34 APIPA 22 functional principle 4 Autozero 28, 40 AVV-marking 47... - Page 54 Index temperature control 3, 28, 40 temperature sensitivity 10 network settings 27 time constant 28, 40 noise 40 transport 39 damage 46 troubleshooting 35 offset 30 operating environment 10 operating ranges 2 unpacking 11 operation 26 Operation Qualification (OQ) 34 optical bench 3 validation 39 optical path 4...
- Page 55 Latest KNAUER instructions online: www.knauer.net/library KNAUER Phone: +49 30 809727-0 Wissenschaftliche Geräte GmbH Fax: +49 30 8015010 Hegauer Weg 38 E-Mail: info@knauer.net 14163 Berlin Internet: www.knauer.net © KNAUER 2019...
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