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Knauer Azura RID 2.1L User Manual

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Detector RID 2.1L
User Manual
V6750

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  • Page 1 Detector RID 2.1L User Manual V6750...

  • Page 2: Table Of Contents

    Table of Contents Table of Contents Note: For your own safety, read the manual and always observe the warnings and safety information on the device and in the manual. Product Information............Device Overview .
  • Page 3 Table of Contents GLP ............... . Starting the Detector .

  • Page 4: Product Information

    Product Information Product Information The AZURA RID 2.1L is a sensitive and competitively priced differential refractometer suitable for detecting compounds with litte or no UV activity such as alcohols, sugars, lipids or polymers. AZURA L features The RID 2.1L is a member in the AZURA L product line and shares a number of common features: ...

  • Page 5: Features

    Product Information Legend Integrator output Service interface (USB) LAN and switch con- nector 1 LAN and switch con- nector 2 Multi-pin connector Connection and main switch Fig. 2 rear view Rear label Features Optical bench The newly designed optical unit with advanced temperature control ensures high sensitivity, fast baseline stabilization, and excellent reproducibility.

  • Page 6: Functional Principle

    Detector Design and Optical Path The AZURA RID 2.1L is a differencial refractive index detector of the deflec- tion type. The detector measures the deflection of a light beam causes by the difference in refractive index between the liquids in sample and refer- ence cell compartments of the flow cell.

  • Page 7: Calculating The Signal Value

    Product Information When the sample cell contains a solution with a different refractive index, the light beam is geometrically proportionally deflected depending on the relative change of the refractive index (according to Snell´s law). This results in a change of the light intensities I and I (one increases and the other decreases), proportional to concentration and refractive index of...

  • Page 8: Intended Use

    Eluents Even though the AZURA RID 2.1L is very resistant to many kinds of com- monly used eluents, you should take care that no eluent or water can get on the device’s surface or even inside the device. Chlorinated hydrocarbons could destroy the varnish and some others (e.

  • Page 9: Safety For Users

    Knowledge regarding the health risks of chemicals  Participation during an installation of a device or a training by the com- pany KNAUER or an authorized company. valid for the specified temperature range not recommended in combination with PEEK small parts and PEEK capillaries not recommended in combination with PEEK small parts and PEEK capillaries RID 2.1L user manual V6750, version 1.0...
  • Page 10 At any time, take the device completely out of operation by either switching off the power switch or by pulling the power plug. Opening the Device The device may be opened by the KNAUER Technical Support or any com- pany authorized by KNAUER only. RID 2.1L user manual V6750, version 1.0...

  • Page 11: Signal Words

    Decontaminatin Report Devices without a completed Decontamination Report will not be repaired. If you would like to return a device to KNAUER, make sure to enclose a com- pleted Decontamination Report with the device: http://www.knauer.net/ en/downloads/service.html...

  • Page 12: Power Connection

    Electronic hazard when using an identically constructed power adapter from another manufacturer.  Contact the Technical Support of KNAUER.  Never connect the device to the power connection. Note: Make sure that power supply and power cables fulfil the criteria (see “Power supply”...

  • Page 13: Unpacking The Device

    Installation  RID 2.1L  user manual DE/EN  power cable  accessory kit RID 2.1L  accessory kit AZURA Unpacking the Device The device is delivered in one closed box. The packaging is selected to pro- tect the device against any possible damage. Prerequisite Check packaging for damage caused during transportation.

  • Page 14: Connecting The Capillaries

    Installation Process Process Figure 3. Connect the nozzle and the fun- nel. 4. Push the other end of the hose onto the nozzle of the leak tray. Fig. 5 Hose connected to device 5. For the bottom device, push the short end of the nozzle into the opening in the collection...

  • Page 15: Integrating The Detector Into A System

    Installation Process Figure 1. Push the capillary through the fitting 2. Slide the lock ring over the tubing. Note that the tapered end of the lock ring has to point to the seal ring 3. Attach the seal ring 4. Push the capillary as far as possi- ble into the detector’s inlet IN 5.

  • Page 16: Computer Control

    Start the system devices according to the instructions given in the respective user manuals. Computer Control The AZURA RID 2.1L can be controlled with different software packages. For reference, please check out the List of Supported Devices on the KNAUER website: http://www.knauer.net/fileadmin/user_upload/produkte/files/...

  • Page 17: Connecting The Cables

    Installation Connecting the Cables A router has several LAN ports and one WAN port that can be used to integrate the LAN into a wide area network (WAN), e.g. a company net- work or the Internet. In contrast, the LAN ports serve to set up a network from devices and a computer .

  • Page 18: Setting A Static Ip Address

    Installation changed and this same number must be entered into the device configura- tion of the chromatography software. We recommend to use the same port number for all devices in the same system. Note: The port is set to 10001 at the factory. You must use the same num- bers in the device configuration of the chromatography software as in the device, otherwise the connection fails.

  • Page 19: Connector Assignments

    Installation Connector Assignments Connector Function Ground for all inputs and outputs. Reference point of the voltage at the signal inputs. 5V OUT Provides a voltage of 5V with respect to GND. This makes it possible to supply a load that is switched by an EVENT. Protection: 5V - 50 mA VALVE 24V Event controlled switching of 24V against...

  • Page 20: Connecting The Cables To The Terminal Strip

    Installation Connector Function FLUSH IN TTL Input - Low active Secure switching threshold at least 10 mA. Activates and deactivates flush valve. EVENT 1 TTL Output (default)/OC with external pull- up up to 24 V (25 mA) Levels - Passive 5V - Active 0V Ground for all inputs and outputs.

  • Page 21: Integrator Connector

    Operation Integrator Connector The integrator output supplies the current signal value as an analog voltage (0–2.5 V). Via software or Mobile Control the signal can be scaled to 0.1 μRIU/mV or 0.5 μRIU/mV (see “Analog Output Scaling and Offset” on page 26).

  • Page 22: Default Settings

    Operation Color Operating condition Operation Fatal error Restart the device. If the fatal error status remains, contact Ser- vice. green Program or sequence is running/was loaded. Center LED does not light Not ready green flashing Equilibration Wait until the device is ready.

  • Page 23: Autozero

    Operation Autozero The autozero command leads to the zeroing of the detector signal. This command can be carried out via software or via analog command. This is a two leveled function. Level one is an electronic or digital zeroing of the sig- nal.

  • Page 24: Led Power Factor

    Operation Peak Width Time Constant Data Rate >0.017 0.05 >0.033 >0.067 >0,167 >0,333 Data Rate The data rate (or sampling rate) is the number of data points per second (Hz) at which the detector transmits data to the computer. The maximum data rate (digital signal) is 100 Hz.

  • Page 25: Extended Dynamic Range

    Extended Dynamic Range The extended dynamic range option enables the linear dynamic range of the AZURA RID 2.1L to be broadened in +100 % (-1000 μRIU offset) or - 100 % (+1000 μRIU offset). When the extended dynamic range function is not activated positive and negative signals up to 1000 μRIU can be detected (A).

  • Page 26: Starting The Detector

    Operation GLP Data Units Explanation Light source Operating time Running hours from light source installation Installation date Light source number Indicates how often the light source has been changed since manufacture. Validation data Last measured span μRIU Last measured span date Leak sensor Serial number Leak sensor serial number...

  • Page 27: Flushing The Flow Cell

    Operation Flushing the Flow Cell The flush function activates the reference cell valve enabling this cell to be purged with eluent. A description of the activated flow path during flush mode is given in a separate section (see “Flow Path” on page 8). The flush valve can be switched on and off immediately either via software or via analog command;...

  • Page 28: Stopping The Detector

    The customer may request the Installation Qualification, which is free of (IQ) charge. In case of a request, the Technical Support of KNAUER or from a provider authorized by KNAUER performs this functionality test during the installation. The Installation Qualification is a standardized document that comes as part of the delivery and includes the following: ...

  • Page 29: Lan

    Troubleshooting Light intensity and balance The detector signal is determined through the calculation formula (see “Sig- nal Value” on page 8) using:  Channel 1 (I ) counts measured on diode 1  Channel 2 (I ) counts measured on diode 2 Before carrying out a measurement, both diodes must be balanced: this means roughly the same amount of light should fall on both diodes, i.e.

  • Page 30: System Messages

     Further measures Install the maintenance software (service tool).  Save device information and send to KNAUER.  Inform the Technical Support. System Messages If other system messages are displayed besides those listed below, please turn the device off and then on. Inform the Technical Support of the manu- facturer in case the system message repeats itself.

  • Page 31: Proceeding With Tasks After A Leak

    Maintenance contract The device may only be opened by the Technical Support of KNAUER or any company authorized by KNAUER. This maintenance work is covered by a separate maintenance contract.

  • Page 32: User Tasks And Intervals

    Maintenance Organic eluents Organic eluents are toxic above a certain concentration. Ensure that work areas are always well-ventilated. When performing maintenance tasks on the device, always wear safety glasses with side protection, protective gloves, and an overall. User Tasks and Intervals GLP data Using the Mobile Control or software you can read out the operating hours of the detector.

  • Page 33: Preparing For Storage Or Transport

    Legal Information Process Process 7. Fill the syringe with the prepared calibration solution. 8. Install the syringe on the IN port. 9. Slowly and carefully inject the sucrose solution into the detector IN port. 10.Wait until the baseline is stable. Result When the baseline is stable, the measured value is read out.

  • Page 34: Warranty Conditions

    2001), old devices manufactured by KNAUER are marked as waste electrical and electronic equipment: 160214. WEEE Registration KNAUER as a company is registered by the WEEE number DE 34642789 in the German "Elektroaltgeräteregister" (EAR). The number belongs to cate- gory 8 and 9, which, among others, comprise laboratory equipment.

  • Page 35: Technical Data

    Technical Data Solvents and Other Operat- All solvents and other operating materials must be collected separately and ing Materials disposed of properly. All wetted components of a device, e. g. flow cells of detectors or pump heads and pressure sensors for pumps, have to be flushed first with isopro- panol and then with water before being maintained, disassembled or dis- posed.

  • Page 36: Repeat Orders

    Repeat Orders Up-to-date information on spare parts and accessories can be found online: www.knauer.net. This list for repeat orders is valid for the time the docu- ment has been published. Deviations afterwards are possible. Note: For repeat orders of spare parts use the enclosed packing list. Contact the Technical Support in case there are any questions on spare parts or accessories.

  • Page 37: Hplc Glossary

    LightGuide KNAUER LightGuide flow cell cartridges combine a maximum light trans- mission (using total reflection) with a minimum cell volume to offer an ideal signal-to-noise ratio. Luer-Lock...
  • Page 38 HPLC Glossary Term Definition retention time The time required from an injection of a substance until the maximum concentration of a substance becomes visible. sample A mixture of different components which are to be separated via chroma- tography. The components are moved by the mobile phase and dissolved from the column.

  • Page 39: Index

    Index Index port problems absorption router additives settings air drafts setup ambient conditions leak analytical leak sensor autozero malfunction AVV marking leak tray backflushing Luer-lock cannula baseline maintenance capillary user fitting Mobile Control lock ring mobile phase chromatogram modifiers contamination noise data rate non-suitability...
  • Page 40 Index software t. control solvent test flammability Installation Qualification line Operation Qualification self-ignition point transport damage tray space requirements ventilation standby vibrations malfunction view storage front suitability rear eluents sunlight warm-up time warranty temperature stability storage zero position RID 2.1L user manual V6750, version 1.0...
  • Page 41 © KNAUER Wissenschaftliche Geräte GmbH All rights reserved. The information in this document is subject to change without prior notice. Original edition, version 1.0 Last update: 11.08.2016 Printed in Germany on environmentally friendly paper from sustainable forests. ® AZURA is a registered trademark of KNAUER Wissenschaftliche Geräte GmbH...

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