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Hanna Instruments HI5221 Instruction Manual
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Hanna Instruments HI5221 Instruction Manual

Ph/mv/ise/temperature bench meters
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pH/mV/ISE/Temperature
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Summary of Contents for Hanna Instruments HI5221

  • Page 1 HI5221 & HI5222 pH/mV/ISE/Temperature Bench Meters...
  • Page 2 Hanna Instruments representative near you at www.hannainst.com. All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

  • Page 3: Table Of Contents

    PRELIMINARY EXAMINATION ....................GENERAL DESCRIPTION ....................... FUNCTIONAL DESCRIPTION ....................SPECIFICATIONS ......................... OPERATIONAL GUIDE ......................DISPLAYING MODES......................SYSTEM SETUP ........................pH SETUP .......................... mV SETUP ......................... ISE SETUP (HI5222 only) ....................pH CALIBRATION ........................ pH MEASUREMENT ......................mV & Relative mV MEASUREMENTS ..................ISE CALIBRATION (HI5222 only) ..................
  • Page 4 • pH Calibration Solutions Kit • Capillary dropper pipette • 12 Vdc Power Adapter • Instruction Manual and Quick Reference Guide • Certificate HI5221-01 HI5222-01 are supplied with 12 Vdc/120 Vac adapter. HI5221-02 HI5222-02 are supplied with 12 Vdc/230 Vac adapter.
  • Page 5 • Single (HI5221) or two (HI5222) input channels; • Capacitive touch keypad; • pH calibration using up to five pH buffers: choose from the list of Hanna Instruments buffers (pH 1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01 and 12.45) or custom buffers (up to 5 custom buffers);...
  • Page 6 HI5221 DESCRIPTION FRONT PANEL REAR PANEL 1) Liquid Crystal Display (LCD) 2) Capacitive touch keypad 3) ON/OFF switch 4) Power adapter socket 5) USB connector 6) BNC electrode connector for pH/ORP measurements 7) Temperature probe socket 8) Reference input socket...
  • Page 7 HI5222 DESCRIPTION FRONT PANEL REAR PANEL 1) Liquid Crystal Display (LCD) 2) Capacitive touch keypad 3) ON/OFF switch 4) Power adapter socket 5) USB connector 6) BNC electrode connector for pH/ORP/ISE measurements (Channel 1) 7) Temperature probe socket (Channel 1) 8) Reference input socket (Channel 1) 9) Reference input socket (Channel 2) 10) Temperature probe socket (Channel 2)

  • Page 8: General Description

    KEYBOARD DESCRIPTION FUNCTION KEYS To enter/exit calibration mode. To select the desired measurement mode, pH, mV, Rel mV (or ISE – HI5222 only). To enter Setup (System, pH, mV or ISE) and to access Log Recall function. To obtain general information about the selected option/operation. VIRTUAL KEYS The upper row keys are assigned to the virtual keys placed on the bottom of the LCD, which allow you to perform the displayed function, depending on the current menu (e.g.
  • Page 9 HI5221 HI5222 -2.0 to 20.0 pH / -2.00 to 20.00 pH / -2.000 to 20.000 pH ±2000.0 mV Range e.g. 10 to 10 M, 0.005 to 10 ppm, 5·10 to 5·10 conc. –7 -20.0 to 120.0 °C / -4.0 to 248.0 °F / 253.2 to 393.2 K 0.1 pH / 0.01 pH / 0.001 pH...
  • Page 10 • Please wait until the instrument finishes the initialization process. Note: It is normal for the loading process to take a few seconds. If the instrument doesn’t display the next screen, restart the meter using the power button. If the problem persists, contact your local Hanna Instruments Office.
  • Page 11 CHANNEL SELECTION (HI5222 only) • Press while in Measure mode to access channel selection menu. Four available options will be displayed: Channel 1, Channel 2, or multi-channel with the first or the second channel focused. The “Choose Channel Configuration” message is displayed in the Reminder messages area.
  • Page 12 Detailed GLP data will be displayed on the LCD for pH Measure and ISE Measure modes only when this option is selected: Last Calibration date and time, Offset and Slope values, Calibration Buffers/Standards and general information regarding the buffers/standards: the calibration temperature, temperature compensation mode, date and time.
  • Page 13 Graph The on-line graph with currently logged values (pH, mV, Rel mV, or ISE vs. Seconds) will be displayed when this option is selected. If there is no active log, the previously logged data for the selected parameter will be shown. To access the off-line / on-line graph: •...
  • Page 14 Log History The measurement, along with Log History, will be visible when this option is selected: 1) The last stored logged data (Not actively logging) or 2) The last data logged from an active logging lot or 3) An empty display - NO LOTS saved, NOT currently logging The log history list also contains the appropriate mV values, the logged temperature, the temperature probe status source, as well as the records time stamp.

  • Page 15: System Setup

    The System Setup menu allows the user to customize the user interface, view meter information, set the external serial communication interface and to restore the manufacturer settings. Accessing System Setup • Press while in Measure mode. • Press . The system setup options will be displayed on the LCD.
  • Page 16 Saving Confirmation Enable this option to force confirmation of a change made to a setting in GLP data option field or a Sample ID name. If Saving Confirmation is enabled, the user will have to accept the change with a key stroke. If Saving Confirmation is disabled, the changes made to these fields change automatically without asking for confirmation GLP Data...
  • Page 17 • Press to return to the GLP Data options. If the Saving Confirmation is enabled, press to accept the modified option, to escape without saving or to return to the editing mode. Otherwise, the modified options are saved automatically. Date & Time Set the current date &...
  • Page 18 Note: If the time is changed with more than one hour before last pH/ISE calibration, a pop-up warning will appear on the LCD, notifying the user that a date/time conflict has occurred and some time-dependent modes could work improperly (e.g. Measure, GLP, Log). LCD Setup This option allows the user to set the Contrast, the Backlight of the LCD and the Backlight Saver.
  • Page 19 • Press and use to highlight the desired color. • Press to confirm your selection and return to the System Setup menu or press to return to the System Setup menu without changing. Language This option allows the user to choose the desired language in which all information will be displayed. To select the Language: •...
  • Page 20 “Factory Calibration Expired” will be displayed when powering up the instrument. The instrument will still function, however, it should be taken to the nearest Hanna Instruments Customer Service for factory calibration. To view the Meter Information: •...
  • Page 21 Software update This function allows the user to update instrument soft- ware. In order to start the PC upgrade application, you need to select the proper baud rate, the software update package and start the update. The pH Setup menu allows the user to set the parameters associated with pH measurement and calibration.
  • Page 22 Profile This option opens the Profile manager. Enabling Profile allows the user to Save, Load or Delete an application Profile. The Profile option allows the user to store up to ten separate profile applications (HI5222 - five profiles for each channel). Each Profile can be named and recalled at a moment’s notice.

  • Page 23: Ph Measurement

    • Select Load Profile to select a profile to use from the list of saved profiles. Highlight the desired profile and press • Select Delete Profile to remove a selected profile from the saved list. Highlight the profile and press Temperature The temperature has a direct influence on pH.
  • Page 24 • Press to confirm your selection (for Temperature Source & Unit options) or press to save the current value (for Manual Temperature option). Otherwise, press to cancel operation. Calibration This option allows the user to setup desired buffers and calibration reminder. Buffer Entry Type Three settings are available for the pH buffers used for electrode calibration: Automatic - the instrument automatically selects the closest buffer to the measured pH value from...
  • Page 25 To set the 1st Cal. Point: • Press while in pH Measure mode. • Press • Use to select the Calibration option. • Press and use highlight the 1st Cal. Point option. • Press as desired. • Press to return to previous menu. Edit Custom Buffers If special custom pH buffers are required during calibra- tion, the Edit Custom Buffers option is available.
  • Page 26 • Press to exit custom buffer edit menu. If the Saving Confirmation is enabled, press to accept the modified option, to escape without saving or to return to the editing mode. Otherwise, the modified option is saved automatically. • Use key to select the next custom buffer to be set or press to return to Calibration options.
  • Page 27 • Press and use to highlight the Calibration Reminder option. • Press and use to highlight the desired option. • Press to confirm your selection or press to cancel operation. Set Reminder Period Schedule the calibration reminder timing with this option (verify Daily or Periodic is set for Calibration Reminder).
  • Page 28 To clear Calibration: • Press while in pH Measure mode. • Press • Use to select the Calibration option. • Press and use highlight the Clear Calibration option. • Press to clear calibration. A pop-up menu will be displayed asking for confirmation (when a calibration is available).
  • Page 29 Edit Sample ID This option allows the user to edit the sample ID. Note: The ID Increment mode must be set to None, to use this feature. To edit the Sample ID: • Press while in pH Measure mode. • Press •...
  • Page 30 Stability Criteria This option allows the user to select the signal stability criteria for the measured parameter (pH, mV, ISE): Fast - this setting will give faster results with less accuracy. Medium - this setting will give medium speed results with medium accuracy. Accurate - this setting will give slower results with high accuracy.
  • Page 31 Note: See section Logging for available types of logging. This option allows the user to edit the logging settings: Logging Type, Logging Data Configuration, Sampling Period and New Lot. Logging Type Three logging types are available: Automatic, Manual and Auto Hold. Automatic - the measurement data is logged automatically at predefined time intervals;...
  • Page 32 To set the Logging Data Configuration: • Press while in pH Measure mode. • Press • Use to select the Log option. • Press and use to highlight the Logging Data Configuration option. • Press and use to highlight the desired parameter to be logged in file. •...
  • Page 33 To generate a New Lot: • Press while in pH Measure mode. • Press • Use to select the Log option. • Press and use to highlight the New Lot option. • Press to generate a new manual lot. A pop-up menu will be displayed asking for confirmation.
  • Page 34 To set the Alarm Limits: • Press while in pH Measure mode. • Press • Use to select the Alarm option. • Press and use highlight the Alarm Limits option. • Press and use to select next/previous entry to be edited. •...
  • Page 35 pH Resolution Select the desired pH resolution with this option. Choose from one (X.X), two (X.XX) or three (X.XXX) digits displayed past the decimals. To set the pH Resolution: • Press while in pH Measure mode. • Press • Use to select the pH Resolution option.
  • Page 36 Profile- See pH Setup section. Temperature ORP measurements are not temperature compensated, although ORP values can change with temperature (e.g. reference electrode potential changes, sample equilibrium changes). It is important to report ORP values together with the reference electrode used and the temperature of measurement.
  • Page 37 Clear Calibration This feature deletes the Relative mV calibration for the selected channel. • Press while in Rel mV mode. • Press then use to access Calibration option. • Press and use to highlight Clear Calibration option. • Press to clear calibration. A pop-up menu will be displayed asking for confirmation (when a calibration is available).
  • Page 38 Profile - See pH Setup section. Reading Mode This option allows the user to select the desired reading mode: Direct, Direct/AutoHold, Known Addi- tion, Known Subtraction, Analyte Addition and Analyte Subtraction. Four of these Reading Modes are collectively known as Incremental Methods (see ISE Theory section for details). Direct measurements and Direct/AutoHold measurements are also available.
  • Page 39 Analyte Subtraction In the Analyte Subtraction method, an aliquot of sample is added to a reactant standard of known concentration and volume. The sample partially reacts with the measured ion. The stoichiometric ratio between standard and sample must be known. The ion concentration is then calculated using the difference in mV potential.

  • Page 40: Ise Calibration (Hi5222 Only)

    Temperature Compensation ISE measurements benefit from temperature compensated corrections if: • standards and sample temperatures differ from each other • the Isopotential Point of the ISE is known. If sample and standards are made at the same temperature, leave this option disabled. Isopotential Point If the Temperature Compensation is enabled, the isopoten- tial point of the ISE must be added in this parameter.
  • Page 41 To set the Manual Entry: • Press while in ISE Measure mode. • Press • Use to select the Calibration option. • Press and use to highlight the Manual Entry option. • Press to select the desired option. Edit Custom Standards Use Edit Custom Standards function to add additional ISE standard values.
  • Page 42 • Use to select the Calibration option. • Press and use to highlight the Edit Standard Group option. • Press and use to choose the ISE standard to be included in the standard group. • Press to add/remove the selected ISE standard to/from the standard group.
  • Page 43 • Press to confirm your selection and return to ISE Setup options. For custom ISE: • Press to edit the Ion Constants for the selected custom ISE. Use to select the desired constant and press to enter edit mode to cancel operation. •...
  • Page 44 Note: If an ISE calibration was performed and a different Ion Selective Electrode is selected (standard or custom), a warning message appears on the LCD informing the user to perform a new calibration or to select the previous ISE in order to perform accurate measurements.
  • Page 45 ISE Significant Digits Accessing this option, the number of ISE significant digits can be set, with one (X), two (XX) or three (XXX) significant digits. To set the ISE Significant Digits: • Press while in ISE Measure mode. • Press •...
  • Page 46 If you are measuring in the acidic range, use pH 7.01 or 6.86 as first buffer and pH 4.01, 3.00 or 1.68 as second buffer. If you are measuring in the alkaline range, use pH 7.01 or 6.86 as first buffer and pH 10.01/9.18 or 12.45 as second buffer.
  • Page 47 • Immerse the pH electrode and the temperature probe approximately 4 cm (1.5”) into a buffer solution of your choice (pH 1.68, 3.00, 4.01, 6.86, 7.01, 9.18, 10.01, 12.45 or a custom buffer) and stir gently. The temperature probe should be close to the pH electrode. •...
  • Page 48 • If the Automatic buffer entry type has been selected for the calibration procedure, the instrument will automatically select the closest buffer to the measured pH value from the edit buffer group (see pH Setup for details). • If the Semiautomatic buffer entry type has been selected for the calibration procedure, the instrument will display only the closest buffers to the measured pH value from all the available buffers and the user must select with the buffer being used.
  • Page 49 Verify the pH electrode and instrument has been calibrated before making pH measurements. DIRECT MEASUREMENT To measure the pH of a sample using the Direct reading mode: • Press and then to select pH Mea- sure mode (select channel first for HI5222). •...
  • Page 50 If the temperature of the sample is known, Manual Temperature Compensation (MTC) can be used by disconnecting the temperature probe. Notes: For single channel pH measurements (HI5221), “MTC” or “ATC” indicators will be displayed on the LCD, while for dual channel pH measurements (HI5222) “MTC1”/”MTC2”...
  • Page 51 mV/ORP MEASUREMENTS Oxidation-reduction potential (ORP) measurements provide the quantification of the oxidizing or reducing power of the tested sample. To correctly perform a redox measurement, the surface of the ORP electrode must be clean and smooth. DIRECT MEASUREMENT To measure the mV of a sample using the Direct reading mode: •...
  • Page 52 Relative mV MEASUREMENTS To measure the Relative mV of a sample: • Press then (select channel first for HI5222). • Verify if a current calibration has been made. • If required, conduct the single point Rel mV calibra- tion. Verify the tip of the electrode is immersed into the known solution or ORP standard.
  • Page 53 For greater accuracy, it is recommended to calibrate the ISE sensors frequently. The instrument should be recalibrated when “ISE x Calibration Expired” (the “x” represents channel “1” or channel ”2”) message appears on the LCD, in the Reminder messages area. Due to electrode conditioning time, the electrode must be kept immersed a few seconds to stabilize.
  • Page 54 The group of calibration standards is set under ISE Setup Calibration. Select standards that are → in the measurement range of the samples. To calibrate the instrument: • Press . If the instrument was calibrated before and calibration was not cleared, the old calibration can be cleared by pressing .
  • Page 55 Press to delete the selected calibrated point and then press to update calibration with the new standard solution. If the isopotential point of the electrode is unknown, the ISE calibration and measurements can be performed without temperature compensation (see ISE Setup, Temperature option for details). When in MTC mode, after selecting a standard press , a pop-up menu will be displayed on the LCD in which the concentration and the temperature...
  • Page 56 Make sure the instrument and ISE sensor have been calibrated before making ISE measurements. When using one of the incremental methods for measurement, at least a two point ISE calibration must be performed to establish the electrode slope. For accurate measurements, add the appropriate ISA (Ionic Strength Adjuster) to both samples and standards.
  • Page 57 • To return to normal Measure mode press Note: If the reading is out of range, “----” will be displayed on the LCD. KNOWN ADDITION To measure the concentration of a sample using the Known Addition incremental method: • Press and then to select ISE Measure mode for the selected channel.
  • Page 58 Start by adding 500 µL at a time (for example). Watch the change in mV. When you have observed approximately a 15 mV change from the original sample, calculate the total volume added. Adjust sample and standard volumes proportionally to standard volumes that can be measured with accuracy.
  • Page 59 • If following an established procedure: Press then edit the method variables and follow the procedure below. • Press to set the method parameters. Press to select next/previ- ous parameter to edit, then press and use to set the desired parameter value.
  • Page 60 • When the reading is stable, press to store the second mV reading. The ISE measurement results will be displayed on the LCD. • Press to log the current results into an ISE Method Report. Press to return to ISE Measure mode. Press to start another measurement.
  • Page 61 • Press then edit the procedure variables to the volumes determined in the prior step. Procedure: • Press to enter Analyte Addition mode. • Volumetrically add standard to a clean beaker. Add a magnetic stir bar and place on a stir plate. Stir standard.
  • Page 62 • If developing a procedure: Before attempting Analyte Subtraction analysis, it is important to determine which sample volume, reactant volume and concentration, will produce the best results and the way the reagent will react with the measured ion on a molar basis (stoichiometric fac- tor).

  • Page 63: Temperature Calibration

    The user temperature calibration menu can be accessed during meter startup by simultaneously pressing three keys as shown in the drawing below. Press the keys after the short beep is heard at the meter power on. Keep all three keys pressed until Temp. Calibration menu appears. Note: The user temperature calibration is performed at three points: around 0 °C, 50 °C and 100 °C.
  • Page 64 There are 5 ways the Reading Mode and Log may be configured together. The table below shows the combinations and indicates where the completed log will be stored. Reading Mode log Recall Automatic (1) Automatic Log Direct Manual (2) Manual Log Auto Hold (NA) Not Applicable Automatic (3)
  • Page 65 2) Direct Reading Mode and Manual Log: Real time continuous measurements are on display and snapshots of measurement data are stored in the Manual log when the user presses . Subsequent snapshots will be added to the same Manual Lot every time the is depressed unless New Lot is selected under Log options.
  • Page 66 5) Direct/AutoHold Reading Mode and Auto Hold Log Press and then keys initiate and automate the capture of stable data which is stored in the Recall Manual Log file. During the process, “AutoHold” will flash until the stability criteria is reached and then the screen freezes in AutoHold mode, the data is logged and marked with an “H”.

  • Page 67: Logging

    • Select the desired lot with press to display the logged data from the highlighted lot. The “Please wait...” message will be displayed on the LCD for one second. The selected Logging Data Configuration options will be displayed on the LCD, together with GLP information (last calibration date and calibrated buffers/standards) if a calibration has been performed on the selected mode and the logged values (measured value, mV...
  • Page 68 The PC software may also be used for real time logging. To allow our users access to the latest version of Hanna Instruments PC compatible software, we made the products available for download at http://software.hannainst.com. Select the product code and...
  • Page 69 ISE THEORY An Ion Selective Electrode (ISE) is an electrochemical sensor that changes voltage with the activity or concentration of ions in solutions. The change in voltage is a logarithmic relationship with concentration, and is expressed by the Nernst equation: E = E + S log(a) where: E - the measured voltage;...
  • Page 70 Some of Hanna Instruments’s Ionic Strength Adjuster formulations also may optimize pH and complex interferences, in addition to standardizing the ionic strength.
  • Page 71 With Known subtraction, a known standard is added to an ionic sample being measured. The standard reacts with the measured ion in the sample in a known manner, thus removing measured ions from the solution. From the change in mV, the concentration of the sample is determined. ·...
  • Page 72 where: C - the sample concentration; ∆E - the difference of potential from the electrode; SAMP S - the electrode slope, determined in a previous - the standard concentration; calibration; - the sample volume; SAMP f - the stoichiometric ratio between sample and - the standard volume;...
  • Page 73 PREPARATION PROCEDURE Remove the protective cap off the pH electrode. SALT DEPOSITS MAY BE PRESENT. This is normal with electrodes. They will disappear when rinsed with water. During transport, tiny bubbles of air may form inside the glass bulb affecting proper functioning of the electrode.
  • Page 74 HI7082 HI8082 for double junction electrodes). Allow the electrode to stand upright for 1 hour. Follow the Storage Procedure above. pH CLEANING PROCEDURE • General Soak in Hanna Instruments HI7061 HI8061 General Cleaning Solution for approximately 30 minutes. • Protein...
  • Page 75 IMPORTANT: After performing any of the cleaning procedures, rinse the electrode thoroughly with distilled water, refill the reference chamber with fresh electrolyte (not necessary for gel-filled electrodes) and soak the electrode in HI70300 HI80300 Storage Solution for at least 1 hour before taking measurements.
  • Page 76 The resistance of glass electrodes partially depends on the temperature. The lower the temperature, the higher the resistance. It takes more time for the reading to stabilize if the resistance is higher. Since the resistance of the pH electrode is in the range of 50 – 200 MΩ, the current across the membrane is in the pico Ampere range.
  • Page 77 pH BUFFER SOLUTIONS HI6016 pH 1.679 Buffer Solution, 500 mL bottle HI6003 pH 3.000 Buffer Solution, 500 mL bottle HI8004L pH 4.01 Buffer Solution in FDA approved bottle, 500 mL HI6004 pH 4.010 Buffer Solution, 500 mL bottle HI8006L pH 6.86 Buffer Solution in FDA approved bottle, 500 mL HI6068 pH 6.862 Buffer Solution, 500 mL bottle HI8007L...
  • Page 78 3.5M KCl + AgCl Electrolyte in FDA approved bottle, 4x30 mL, for single HI8071 junction electrodes HI8072 1M KNO Electrolyte in FDA approved bottle, 4x30 mL HI8082 3.5M KCl Electrolyte in FDA approved bottle, 4x30 mL, for double junction electrodes HI8093 1M KCl + AgCl Electrolyte in FDA approved bottle, 4x30 mL ORP SOLUTIONS...
  • Page 79 HI1083B Glass body, micro, Viscolene, non refillable, combination pH electrode. Use: biotechnology, micro titration. HI1131B Glass body, refillable, double junction, combination pH electrode. Use: general purpose. HI1330B Glass body, semimicro, single junction, refillable, combination pH electrode. Use: laboratory, vials. HI1331B Glass body, semimicro, single junction, refillable, combination pH electrode.
  • Page 80 HI2031B Glass body, semimicro, conical, single junction, refillable, combination pH electrode. Use: semisolid products. HI1332B Plastic body (PEI), double junction, refillable, combination pH electrode. Use: general purpose. HI1413B Glass body, single junction, flat tip, Viscolene, non refillable, combination pH electrode. Use: surface measurement.
  • Page 81 FC210B Glass body, double junction, conical, Viscolene, non refillable, combination pH electrode. Use: milk, yogurt. FC220B Glass body, triple ceramic, single junction, refillable, combination pH electrode. Use: food processing. FC911B Plastic body (PVDF), double junction, refillable with built-in amplifier, combination pH electrode. Use: very high humidity.

  • Page 82: Accessories

    HI4430B Plastic body (PEI), gel filled, combination gold ORP electrode. Use: general purpose. Consult the Hanna Instruments General Catalog for more electrodes with screw-type or BNC connectors. EXTENSION CABLE FOR SCREW-TYPE ELECTRODES (SCREW TO BNC ADAPTER) HI7855/1 Extension cable 1 m (3.3’) long HI7855/3 Extension cable 3 m (9.9’) long...
  • Page 83 If the repair is not covered by the warranty, you will be noticed of the charges incurred. If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization (RGA) number from the Technical Service department and then send it with shipping costs prepaid.
  • Page 84 World Headquarters Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA www.hannainst.com MAN5222 Printed in ROMANIA...

This manual is also suitable for:

Hi5222Hi5221-01Hi5221-02Hi5222-01Hi5222-02
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