horiba HEMAPREP RAB316AEN User Manual
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horiba HEMAPREP RAB316AEN User Manual

Blood spreading system
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Blood Spreading System
User Manual
Ref: RAB316AEN

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Summary of Contents for horiba HEMAPREP RAB316AEN

  • Page 1 Blood Spreading System User Manual Ref: RAB316AEN...
  • Page 3 User Manual User Manual Ref: RAB316AEN...

  • Page 5: Table Of Contents

    Contents Foreword ............................3 1. Revisions ............................ 4 2. Legal Information ........................5 Introduction ........................... 9 1. Warning and Precautions ....................10 2. Operational Conditions ...................... 12 3. Labels and Connections ....................14 Specifications ..........................15 1. Intended Use ...........................16 2. Principles of Operation .......................17 3.
  • Page 6 User Manual Ref: RAB316AEN...

  • Page 7: Foreword

    ............................ 4 2. Legal Information ........................5 2.1. Declaration of Conformity......................5 2.2. Notice of Liability..........................5 2.3. Trademarks............................ 5 2.4. Graphics............................5 2.5. Document Symbols........................6 2.6. Typographical Conventions......................6 2.7. Copyright © 2016 by HORIBA ABX SAS..................6 User Manual Ref: RAB316AEN...

  • Page 8: Revisions

    Foreword Revisions Revisions Reference Internal Reference Document Date Issued RAB316AEN November 2016 This document is only available online at www.horiba-abx.com/documentation. User Manual Ref: RAB316AEN...

  • Page 9: Legal Information

    The information in this manual is distributed on an "As Is" basis, without warranty. While every precaution has been taken in the preparation of this manual, HORIBA Medical will not assume any liability to any persons or entities with respect to loss or damage, caused or alleged to be caused directly or indirectly by not following the instructions contained in this manual, or by using the hardware products described herein in a manner inconsistent with our product labelling.

  • Page 10: Document Symbols

    2.7. Copyright © 2016 by HORIBA ABX SAS All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of HORIBA Medical.
  • Page 11 Foreword Legal Information HORIBA ABX SAS Parc Euromédecine - Rue du Caducée B.P. 7290 34184 MONTPELLIER Cedex 4 - FRANCE Phone: +33 (0)4 67 14 15 16 Fax: +33 (0)4 67 14 15 17 User Manual Ref: RAB316AEN...
  • Page 12 Foreword Legal Information User Manual Ref: RAB316AEN...

  • Page 13: Introduction

    Introduction 1. Warning and Precautions ....................10 1.1. Limited Warranty.......................... 10 1.2. Operational Precautions.......................11 1.3. Biological Hazard......................... 11 1.4. Graphics and Symbols.........................11 2. Operational Conditions ...................... 12 2.1. Environmental Protection......................12 2.2. Installation............................ 12 2.3. Package............................12 3. Labels and Connections ....................

  • Page 14: Warning And Precautions

    ■ The system is operated according to HORIBA Medical recommendations. ■ Proper tools are used when maintenance or troubleshooting operations are performed. If this system has been supplied to you by anyone other than HORIBA Medical or an authorized representative, HORIBA Medical cannot guarantee this product in terms of specification, latest revision and latest documentation.

  • Page 15: Operational Precautions

    Introduction Warning and Precautions 1.2. Operational Precautions ■ Gently manipulate the front lever. ■ When using frosted slides, make sure frosted portion is facing front. If not, spreader blades come into contact with frosting and become nicked. ■ Clean the spreader blades between each operation especially if an excess of blood was applied on the slides, to prevent carry-over.

  • Page 16: Operational Conditions

    Installation Package content: ■ HEMAPREP ■ Spreader blades Only HORIBA Medical approved accessories should be used with the HEMAPREP. 2.3. Package Factory package of the system HEMAPREP and its implements consists of firm corrugated cardboard and polyethylene foil. Package protects the system and its implements from adverse factors of outside environment.
  • Page 17 Introduction Operational Conditions The system must be transported in its original factory package. After unpacking, we recommend you to: ■ Visually check the system ■ Check the spread quality on first using User Manual Ref: RAB316AEN...

  • Page 18: Labels And Connections

    Introduction Labels and Connections Labels and Connections 3.1. Serial Number Label The serial label is located below the system. 3.2. Warnings and Biological Hazards Labels Warning! Biological hazard Front of the system Risk: the specimens containing human specimen extracts are potentially infectious; all accessible surfaces of the system can be potentially contaminated.

  • Page 19: Specifications

    Specifications 1. Intended Use ...........................16 2. Principles of Operation .......................17 3. Performance Characteristics ...................18 User Manual Ref: RAB316AEN...

  • Page 20: Intended Use

    Specifications Intended Use Intended Use The HEMAPREP is a mechanical system designed to emulate the manual method of preparing peripheral blood films by the wedge technique. The system was created for both stationary or portable use. ■ As stationary use, it is valuable for preparations in laboratories or at fixed locations. ■...

  • Page 21: Principles Of Operation

    Specifications Principles of Operation Principles of Operation The system is lever operated and does not require an external power source or battery. 1. Glass spreader blades are mechanically brought into contact with the drop of blood. 2. The system stops to allow the blood to moisten the spreader blades. 3.

  • Page 22: Performance Characteristics

    Specifications Performance Characteristics Performance Characteristics The HEMAPREP can achieve reproducible smears with ample working area, good distribution and minimization of trauma because the smearing action is automatically controlled. It produces borders along the edges of the slide which facilitates inspection of these areas when used in accordance with these instructions.

  • Page 23: Workflow

    Workflow 1. General Recommendations ....................20 1.1. Blood Sample Collection......................20 1.2. Special Smears..........................20 2. Adjusting the System before Operation ..............22 2.1. To Install the System........................22 2.2. To Calibrate the System.......................22 2.3. To Determine the Pause Control Time..................23 3.

  • Page 24: General Recommendations

    Workflow General Recommendations General Recommendations 1.1. Blood Sample Collection Accepted types of blood The system accepts both fresh blood and blood collected in anti-coagulant as EDTA. We recommend you to perform operation as soon as possible after collection. EDTA can cause morphological damages after four hours.
  • Page 25 Workflow General Recommendations Lupus Erythematosus Preparation We recommend you to deliver a small quantity of the prepared buffy-coat material on the target and to set the smear control button at its slowest settings. You must clean the spreader blades after each specimen. Related information: ■...

  • Page 26: Adjusting The System Before Operation

    Workflow Adjusting the System before Operation Adjusting the System before Operation 2.1. To Install the System The HEMAPREP system is shipped with glass blades assembled. 1. Place the system in horizontal position or on a level surface. The system must not be placed near a vibrating device. 2.

  • Page 27: To Determine The Pause Control Time

    Workflow Adjusting the System before Operation 2.3. To Determine the Pause Control Time The pause control determines the time that the spreader blades stay in contact with the blood drop. The pause control screw is located below the system and is factory adjusted. Never completely tighten the screw during the adjustment.

  • Page 28: Performing The Test

    Workflow Performing the Test Performing the Test 3.1. To Spread the Samples You must have cleaned and placed the slides on the system. 1. Locate the target locations indicated by marks on the trays. 2. Deliver a drop of blood on the indicated marks on the slides, with approximately the same diameter.

  • Page 29: To Clean The Spreader Blades

    Workflow Performing the Test 5. Remove the slides and check the spread quality. 6. Clean the spreader blades using a tissue dampened with a disinfectant product. 3.2. To Clean the Spreader Blades Never immerse the system. 1. Rotate the spreader arm backwards to reach its rest position. 2.
  • Page 30 Workflow Performing the Test User Manual Ref: RAB316AEN...

  • Page 31: Maintenance And Troubleshooting

    Maintenance and Troubleshooting 1. Cleaning Procedure ......................28 1.1. Cleaning Frequency........................28 1.2. To Clean the Spreader Blades..................... 28 2. Replacement Procedures ....................29 2.1. Consumables..........................29 2.2. To Replace the Spreader Blades....................29 2.3. To Replace the Spreader Holders....................30 3. Troubleshooting Procedures ...................

  • Page 32: Cleaning Procedure

    Maintenance and Troubleshooting Cleaning Procedure Cleaning Procedure 1.1. Cleaning Frequency The laboratory has to determine the spreader blades cleaning frequency. A small number of cells remain on the spreader blades. Experiments run by the original manufacturer show that approximately five to ten leukocytes have carried over from one smear to the next, where they typically are mixed with approximately 20000 leukocytes.

  • Page 33: Replacement Procedures

    SLIDE TRAYS INTERNATIONAL 1300029914 HEMAPREP is made of rugged material and does not require periodic adjustment or lubrication. For more detailed information, please contact your local HORIBA Medical representative. 2.2. To Replace the Spreader Blades You need the following elements to perform this procedure: ■...

  • Page 34: To Replace The Spreader Holders

    Maintenance and Troubleshooting Replacement Procedures 1. Hold the spreader assembly and remove the spreader blade using the thumb and forefinger. 2. Remove the protective paper of the new spreader blade provided with the system. 3. Insert the new spreader blade in the spreader assembly and firmly press. Check the spread quality after spreader blades replacement.

  • Page 35: Troubleshooting Procedures

    Maintenance and Troubleshooting Troubleshooting Procedures Troubleshooting Procedures 3.1. Non Homogeneous Smears The smears appear: ■ erratic with a rough edge ■ with streaks ■ with a dip in the center of the feathered edge Cause Solution Do not hold the lever down too long. Release it as soon as it reaches its bottom position.

  • Page 36: Excessively Short Smears

    Maintenance and Troubleshooting Troubleshooting Procedures 3.3. Excessively Short Smears Cause Solution The blood drop is too small. Apply a larger blood drop. The smear is too thick or the blood has high Rotate the smear control button in 1/8 of a turn clockwise until hematocrit.

  • Page 37: Incomplete Pickup Of Blood Drop

    Maintenance and Troubleshooting Troubleshooting Procedures 3.7. Incomplete Pickup of Blood Drop Cause Solution Deliver the drop of blood directly on the target location. Spreader blade is not in contact with blood drop. If insufficient blood is still picked up, deliver the drop slightly The drop was delivered behind the target area.
  • Page 38 Maintenance and Troubleshooting Troubleshooting Procedures User Manual Ref: RAB316AEN...
  • Page 39 Index Serial number label, 14 Adjustment, 22 Special smears Calibration, 22 Bad spread, 33 Installation, 22 Bullet-shaped feather edge, 32 Pause control, 23 Bullet-shaped smears, 32 Incomplete pickup, 33 Long smears, 31 Cleaning, 28 Lopsided smears, 32 Frequency, 28 Non homogeneous, 31 Spreader blades, 25, 28 Sample collection, 20 Consumables, 29...
  • Page 40 User Manual Ref: RAB316AEN...

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