Trace2O Metalyser HM5000 Instruction Manual
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Trace2O Metalyser HM5000 Instruction Manual

Benchtop laboratory heavy metals analyser
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METALYSER HM5000 BENCHTOP
Laboratory Heavy Metals Analyser

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Summary of Contents for Trace2O Metalyser HM5000

  • Page 1 METALYSER HM5000 BENCHTOP Laboratory Heavy Metals Analyser...

  • Page 2: Table Of Contents

    Metalyser HM5000 Benchtop Instruction Manual v1.0 CONTENTS 1. INTRODUCTION 2. BOX CONTENTS 3. GETTING TO KNOW YOUR METALYSER 4. INSTRUMENT INSTALLATION 5. SENSOR MODULE 6. CONNECTION SETUP 7. INTRODUCING METAWARE 8. USING THE METAWARE 9. GRAPH TAB 10. SPREADSHEET TAB 11.
  • Page 3 www.trace2o.com...

  • Page 4: Introduction

    HM5000 on the handheld instrument. The measurement parameter file must be submitted to the Trace2o technical department.The file will then be converted for use on the handheld instruments by one of our team, who will liaise with you to ensure complete compatibility with your instrument.

  • Page 5: Box Contents

    • Adapters and hose barb set • Mains power adapter • USB to 12V car charger adapter In the unlikely event that any of these items are missing, contact Trace2o or your local distributor at your first opportunity. www.trace2o.com...

  • Page 6: Getting To Know Your Metalyser

    Metalyser HM5000 Benchtop Instruction Manual v1.0 3. GETTING TO KNOW YOUR METALYSER On/ Off switch Sensor Module Sample Beaker Platform www.trace2o.com...
  • Page 7 Metalyser HM5000 Benchtop Instruction Manual v1.0 USB Connector Inlet port 12V DC Regulator www.trace2o.com...

  • Page 8: Instrument Installation

    Remove all of the packaging from the HM5000 instrument and ensure that all of the contents are present. If any items appear to be missing, please contact your local distributor or Trace2o. Set the instrument on a firm level surface.
  • Page 9 Metalyser HM5000 Benchtop Instruction Manual v1.0 Option 3: 10mm I/D Flexible tubing The supplied 20cm length of 4mm O/D rigid tubing should be connected to the N inlet port on the back of the HM5000. The supplied adapter and hose barb can then be used to connect this to 10mm I/D flexible tubing.

  • Page 10: Sensor Module

    These are intended to protect the electrode surfaces. They should be removed during analysis, and replaced when the instrument is not in use. Trace2o recommends removing the reference electrode from the sensor module for storage. When replacing the protective cap for the reference electrode, fill with deionised water, as this will prevent the reference electrode from drying out.
  • Page 11 Metalyser HM5000 Benchtop Instruction Manual v1.0 Connecting/disconnecting the sensor module The sensor module can be disconnected and removed if necessary to facilitate cleaning of the HM5000 instrument. To disconnect the sensor module from the body of the instrument, turn the sensor module cogwheel counter-clockwise to unlock, and carefully pull gently downwards.
  • Page 12 Metalyser HM5000 Benchtop Instruction Manual v1.0 To reconnect the sensor module, plug the 4mm nitrogen tubing into the in-line connector. Connect plug A to socket B of the blue cable connector and tighten the locking ring. Ensure that the working electrode socket is located towards the front of the sensor module, then align the twist-lock pins with the slots on the instrument, slide the sensor module into place, and twist clockwise to lock.
  • Page 13 Metalyser HM5000 Benchtop Instruction Manual v1.0 Connecting/disconnecting the electrodes The electrodes are all pre-fitted, but may need to be removed during the course of analysis, for example when switching between the two working electrodes. To fit the electrodes, simply align the white arrow on the black connector shroud with the arrow on the socket and gently push the electrode.
  • Page 14 Metalyser HM5000 Benchtop Instruction Manual v1.0 The working electrode has a 6-pin connector and will only fit into the W position. The counter and reference electrodes both have 3-pin connectors. The instrument requires all three electrodes in the correct positions to function correctly.
  • Page 15 Metalyser HM5000 Benchtop Instruction Manual v1.0 Only use the supplied reference electrode fill solution. Fill solution Fill solution level at max level too low Remember to remove the protective rubber cap before use, and to replace the cap after use to prevent the reference electrode from drying out. Add a few drops of deionised water to the cap before placing on the reference electrode.

  • Page 16: Connection Setup

    6. CONNECTION SETUP Connecting a PC to the Metalyser HM5000 The Metalyser HM5000 can connect to a PC via either USB or Bluetooth. If, after initial setup the user later decides to connect via another method, initial setup will need to be repeated to re-establish the connection.
  • Page 17 Metalyser HM5000 Benchtop Instruction Manual v1.0 Bluetooth Installation Your HM5000 instrument is able to communicate with a PC through Bluetooth. Most newer laptops and some desktops will have built-in Bluetooth connectivity; however, a Bluetooth adapter dongle is supplied with the instrument. Follow the procedure to correctly establish a connection to the instrument via Bluetooth.

  • Page 18: Introducing Metaware

    Metalyser HM5000 Benchtop Instruction Manual v1.0 7. INTRODUCING METAWARE Loading Metaware • Go to Start Menu • Select Programs • Select Trace2o • Click Trace2o Metaware Benchtop. Control Panel Tools Menu File Menu SpreadsheetTab Graph Tab Method Parameter Setup Tab...
  • Page 19 Options, and check the Serial Ports checkbox on the dialog box. Click OK, then look for Metalyser HM5000 (COMX) (where X is the numerical value of the COM port to which your Metalyser is connected). in the Communications sub-menu.
  • Page 20 Metalyser HM5000 Benchtop Instruction Manual v1.0 If connecting via Bluetooth Select Tools, then Communications, then Metalyser 5XX-XX-XXX (where the X values correspond to the serial number of your instrument). The connection status panel on the info pane will change from ‘Disconnected’ to ‘Connecting..’...

  • Page 21: Using The Metaware

    Metalyser HM5000 Benchtop Instruction Manual v1.0 8. USING THE METAWARE Loading a method parameter file The HM5000 Installation CD includes a selection of method parameters and template files to get you started. Refer to the application notes provided with the instrument for information on how to carry out an analysis using these files.
  • Page 22 Metalyser HM5000 Benchtop Instruction Manual v1.0 The control panel The control panel is used for controlling the stirrer and the height of the adjustable platform, as well as setting the number of repeats, and starting the analysis. Stirrer The stirrer will run automatically throughout the conditioning, deposition and equilibrium steps of an analysis.
  • Page 23 Metalyser HM5000 Benchtop Instruction Manual v1.0 Platform The sample elevation platform must be lowered to facilitate insertion or removal of a sample, or addition of a standard. The platform must be raised during analysis so that the electrodes are submerged in the sample.
  • Page 24 Method Parameter Setup tab. For more information on appropriate usage of the deposition time box, consult the relevant Trace2o application note for the analysis. The Plot Results checkbox is intended for use when using the potentiostat for purposes other than analysis - for example, thin film plating of the working electrode.

  • Page 25: Graph Tab

    Metalyser HM5000 Benchtop Instruction Manual v1.0 9. GRAPH TAB Once an analysis has run the voltammogram will be displayed and several functions can then be performed. To manually find the cursor position for peak position and height, choose view from the top menu and then select cross hair value.
  • Page 26 Metalyser HM5000 Benchtop Instruction Manual v1.0 Advanced It is possible to measure the peak heights manually to provide raw current data which can assist with diagnostics and facilitate more accurate data analysis. • Calculate Peak: To calculate a peak, first position the cursor over the data-point you wish to use as the right hand side of the peak then right click the mouse and click ‘calculate peak’.
  • Page 27 To subtract the baseline from subsequent analyses, right click on the file you wish to perform a background subtraction on, and click ‘Subtract baseline’. NB: The contents of the “File” menu will change according to which tab is currently in use. www.trace2o.com...

  • Page 28: Spreadsheet Tab

    Metalyser HM5000 Benchtop Instruction Manual v1.0 10. SPREADSHEET TAB The HM5000 has a built-in spreadsheet function enabling the user to perform linear regression on the analysis data as well as allowing the creation of a calibration curve for use on further samples.
  • Page 29 Metalyser HM5000 Benchtop Instruction Manual v1.0 The table has four possible entries for the sample concentration. The first entry would normally be 0 as this is the unknown concentration of the sample that is trying to be establshed. The second, third and fourth entries would be the sample concentration after each addition of a standard.
  • Page 30 Metalyser HM5000 Benchtop Instruction Manual v1.0 Calibration The data calculated above is a calibration curve for the sample being analysed. If desired, this calibration can now be applied to new samples. To do this, some extra data is first required.

  • Page 31: Measurement Techniques

    Metalyser HM5000 Benchtop Instruction Manual v1.0 11. MEASUREMENT TECHNIQUES The HM5000 is capable of running 4 different electrochemical voltammetric analysis techniques; Linear Sweep, Differential Pulse, Square Wave and Cyclic Voltammetry. The following gives a brief explanation of these techniques. Linear Sweep...

  • Page 32: Differential Pulse

    Metalyser HM5000 Benchtop Instruction Manual v1.0 Differential Pulse Potential Time Differential Pulse is essentially a linear sweep scan with a voltage pulse at each of the voltage steps. The values of Epulse and Tpulse set the amplitude and time of the pulses. The increased time at which the redox potential is held increases the current and provides a more sensitive technique.

  • Page 33: Square Wave

    Metalyser HM5000 Benchtop Instruction Manual v1.0 Square Wave Potential Time Square Wave voltammetry is also a derivative of linear sweep with a square wave super-imposed on the ramp but the centre of each square wave pulse is centred on the step potential. This method is the most effective against the effect of capacitive current and increases the signal to noise ratio giving lower detection limits.

  • Page 34: Cyclic Voltammetry

    Metalyser HM5000 Benchtop Instruction Manual v1.0 Cyclic Voltammetry Potential Time Cyclic Voltammetry is a reversible linear sweep. The scan starts at the potential Ebegin and scans to Elow at which point it reverses and scans positive to Eend before reversing again and scanning back to the start potential.

  • Page 35: Table Of Electrochemical Parameters

    Metalyser HM5000 Benchtop Instruction Manual v1.0 Electrochemical Parameters The following table shows which method parameters are available with each of the four techniques outlined earlier. The drop down menu on the method parameter tab selects the technique and only those parameters available for that technique will be shown.

  • Page 36: Method Sequence

    Metalyser HM5000 Benchtop Instruction Manual v1.0 Method sequence Once the method parameters have been set up the analytical scan can be run. The scans run in the following sequence: 1. Conditioning Step 2. Deposition Step 3. Equilibration Step at Ebegin 4.

  • Page 37: User Configurable Parameters & Ranges

    Metalyser HM5000 Benchtop Instruction Manual v1.0 User configurable parameters and ranges Min current/Max current - The current range should be optimised for the height range of peaks that are obtained. Run a preliminary scan with Min current set to 1nA and Max current set to 1mA to judge the expected current range of peak heights, then set both to create a window.

  • Page 38: Care And Maintenance

    Instrument cleaning Trace2o strongly recommends the use of IPA wipes for routine cleaning of the Metalyser HM5000 Benchtop instrument, sensor module, and electrodes. Other polar protic solvents (i.e. methanol, water) are also suitable for cleaning the instrument.
  • Page 39 Metalyser HM5000 Benchtop Instruction Manual v1.0 Care of your electrodes Counter electrode – an occasional quick visual inspection is required to ensure that the electrode has no physical damage. Reference electrode –regularly perform a visual inspection to ensure that the electrode has no physical damage, and that there is sufficient liquid (at least 1/3rd full) in the reference chamber, and no bubbles in the inner tube.
  • Page 40 Trace2o Technical Support or your distributor in the first instance. Trace2o products are provided with a full one year warranty. It is recommended that your HM5000 is returned to your local dealer for an annual service to keep the instrument in good condition.

  • Page 41: Specifications

    Metalyser HM5000 Benchtop Instruction Manual v1.0 13. SPECIFICATIONS Metalyser HM5000 Benchtop Unit Power supply: 12V DC, 1.5A, 2.1mm DC Jack Nitrogen inlet: 4mm push fit Nitrogen outlet: 4mm PTFE rigid tube Nitrogen regulator: Max pressure 10Bar Materials: ABS-ESD, PC, PEEK, PTFE...

  • Page 42: Appendix 1: Power Supply Frequency

    Metalyser HM5000 Benchtop Instruction Manual v1.0 APPENDIX 1: POWER SUPPLY FREQUENCY Regardless of the measurement technique used, the power supply frequency appropriate to the country of use must be set, to either 50 Hz or 60 Hz This eliminates interference from fluctuations in mains AC power supply. Set the power supply frequency to that of your country.
  • Page 43 Metalyser HM5000 Benchtop Instruction Manual v1.0 50 Hz Norway Rwanda Slovakia Tajikistan Ukraine Oman St. Lucia Slovenia Tanzania Pakistan Saint Pierre and Miquelon Somalia Thailand United Kingdom Papua New Guinea St. Vincent & Grenadines South Africa Timor-Leste Uruguay Paraguay Samoa...

  • Page 44: Appendix 2: Accessories & Consumables

    Metalyser HM5000 Benchtop Instruction Manual v1.0 APPENDIX 2: ACCESSORIES & CONSUMABLES Replacement parts Replaceable sensor module (electrodes to be ordered separately) HMA110051 Working electrode* HMA110002 Reference electrode* HMA110004 Counter electrode* HMA110005 Rechargeable battery kit (battery, leads and charger) HMA120011 Mains adapter*...
  • Page 45 HMC401000 M3 Buffer sachets Solution 100mL HMC103200 for 200 tests (As) M2 & M3 Conditioning HMC402000 M6 Buffer pack for Solution 100mL HMC106050 50 tests (Mn) M6 Conditioning HMC406000 M6 Buffer pack for Solution 100mL HMC106200 200 tests (Mn www.trace2o.com...
  • Page 46 Metalyser HM5000 Benchtop Instruction Manual v1.0 NOTES 46 www.trace2o.com...
  • Page 47 Metalyser HM5000 Benchtop Instruction Manual v1.0 NOTES www.trace2o.com...
  • Page 48 Technology Centre Unit 4, Transigo Gables Way Thatcham Berkshire RG19 4ZA United Kingdom Tel. +44 (0) 1635 866772 Fax +44 (0) 1635 873509 info@trace2o.com www.trace2o.com...

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