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Summary of Contents for Thermo Electron SORVALL RC3BP
- Page 1 ® SORVALL ™ RC 3 BP OPERATING INSTRUCTIONS PN 56311-6...
- Page 2 RC3 BP™ Table of Contents OPERATING INSTRUCTIONS ™ ® SORVALL RC3 BP Lowspeed Refrigerated Centrifuge Thermo Electron Corporation Asheville, North Carolina U.S.A. PN 56311-6 Issued February 2007...
- Page 3 IBM is a registered trademark of International Business Machines Corporation; Pentium is a registered trademark of Intel Corporation; Microsoft, MS, DOS, Windows, Windows 95, and Windows NT are registered trademarks of Microsoft Corporation. © 1996, 1997, 1998, 2003, by Thermo Electron Corporation...
- Page 4 RC3 BP™ Table of Contents Important Safety Information Certain potentially dangerous conditions are inherent to the use of all centrifuges. To ensure safe operation of this centrifuge, anyone using it should be aware of all safe practices and take all precautions described below and throughout this manual.
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Page 5: Table Of Contents
® Table of Contents SORVALL Centrifuges Table of Contents Page Safety Information Page ....... . iii Chapter 1. - Page 6 RC3 BP™ Table of Contents Page Rotor Temperature Equilibration ......4-11 Running Hazardous Material ......4-12 Reducing Speed for Rotor Compartment Loads in Excess of Design Mass .
- Page 7 ® Table of Contents SORVALL Centrifuges...
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Page 8: Chapter 1. Introduction & Description
RC3 BP™ Introduction & Description Chapter 1: INTRODUCTION & DESCRIPTION This manual contains information required to operate and maintain the SORVALL ® RC3BP™ Lowspeed Refrigerated Centrifuge. If you require additional information regarding operation or maintenance, please contact Thermo for assistance. In the United States, call Thermo toll-free 1-800-522-7746; outside the United States, contact the nearest Thermo office (see back cover) or your local representative for SORVALL ®... - Page 9 ® Introduction & Description SORVALL Centrifuges Specific RC3BP™ features include: • An optional Network Computer Interface Package, which includes WatchLog Network™ software, allows automatic computerized logging of centrifuge QC RUN and production run data via RS485/RS232 communication hardware. • QC RUN feature allows simple, automatic speed/temperature verification (optional Computer Interface Package required).
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Page 10: Parts Location And Identification
RC3 BP™ Introduction & Description • SLOW STOP chooses gentle deceleration from 500 to 0 rpm (normal braking from set speed transitions to a more gradual rate at 500 rpm), with the rate defined by selection of one of ten different braking profiles. -
Page 11: Centrifuge Specifications
® Introduction & Description SORVALL Centrifuges Centrifuge Specifications Speed Maximum Selectable Speed 6000 rpm Maximum Relative Centrifugal Force 7277 g ±1% or 20 rpm, Speed Control Accuracy whichever is greater Time Maximum Selectable Time 99 minutes, 59 seconds (or HOLD for indefinite length of time) Maximum Selectable ACE (Accumulated Centrifugal Effect™) -
Page 12: Optional Network Computer Interface Package
RC3 BP™ Introduction & Description Supply Power Requirements Supply Configurations (AC) 200 V, 50 Hz, 1Ø 200 V, 60 Hz, 1Ø 208 V, 60 Hz, 1Ø 220 V, 50 Hz, 1Ø 220 V, 60 Hz, 1Ø 230 V, 60 Hz, 1Ø 230 V, 50 Hz, 1Ø... -
Page 13: Parts Supplied
® Introduction & Description SORVALL Centrifuges NOTE The optional Network Computer Package is to be ini- tially installed by Thermo or your local representative for ® SORVALL products. If any of the network centrifuges or the computer will not all be in the same room, the customer is required to preinstall an appropriate length of 4-wire RJ11 telephone line between rooms before scheduling installation. -
Page 14: Chapter 2. Installation
RC3 BP™ Installation Chapter 2: INSTALLATION This chapter contains instructions to prepare the SORVALL ® RC3BP™ centrifuge for operation. Inspection 1. When you receive your centrifuge, carefully inspect it for any signs of shipping damage. If you find damage, report it imme- diately to the transportation company and file a damage claim, then notify Thermo. -
Page 15: Location
® Installation SORVALL Centrifuges 30 AMP CIRCUIT PROTECTOR WALL OUTLET MEASURED LINE VOLTAGE 30 AMP CIRCUIT PROTECTOR Figure 2-1. RC3BP™ Electrical Requirements Single phase RC3BP™ Centrifuges are equipped with a three-wire cord with three-prong connector to fit a NEMA 6-30P receptacle, or, on 230V 50Hz centrifuges, an IEC 60309-type 3-pin receptacle (32A, 2-pole and earth). -
Page 16: Environment
RC3 BP™ Installation 102 cm Figure 2-2. Centrifuge Dimensions Environment Ambient air temperature at the centrifuge air inlets must be be- tween 15°C to 38°C (59°F to 100°F), with relative humidity ≤90%, for the centrifuge to operate. Ideal ambient temperature is between 20°C to 30°C (68°F to 86°F), therefore, avoid areas near heat sources (for example, heating pipes and radiators). -
Page 17: Installation
® Installation SORVALL Centrifuges Installation A Preinstallation Kit (Catalog No. 22001) is supplied for UL/CSA/ CE compliance. Install the Preinstallation Kit according to instruc- tions supplied with the kit. To install the RC3BP™: 1. Position the RC3BP™ in an operating location that satisfies the criteria specified in the previous Location paragraph. -
Page 18: Chapter 3. Controls, Indicators, And Displays
RC3 BP™ Controls, Indicators, and Displays Chapter 3: CONTROLS, DISPLAYS and INDICATORS The RC3BP™ controls, displays and indicators have been designed for ease of use – the control panel (figure 3-1) allows entry with visual verification of set parameters and current conditions, prompts action if an entry is inappropriate (remains blinking), and communicates status with user messages. -
Page 19: Run Display
® Controls, Indicators, and Displays SORVALL Centrifuges RUN Display SPEED TIME TEMPERATURE °C MIN / SEC The RUN display is the dark grey area toward the top of the control panel, and is comprised of three fields of large red LEDs that indicate the current measured/calculated conditions during a run: •... -
Page 20: Set Display
RC3 BP™ Controls, Indicators, and Displays Between runs, the RUN display shows the previous run duration value, viewable in each control mode. Pressing TIME shows the MIN:SEC value at termination (the SET display shows the last TIME input value). HOLD shows the total MIN:SEC value after deceleration to zero. -
Page 21: Primary Function Keys And Home Screen Fields
® Controls, Indicators, and Displays SORVALL Centrifuges Primary Function Keys and HOME Screen Fields TIME TEMP ROTOR RECALL SPEED HOLD The Primary Function keys are the blue keys below the SET display. Each key is positioned below a corresponding field in the HOME screen, allowing simple, direct access to basic run parameter controls: ROTOR is pressed to specify a different rotor than the one shown in the SET display, as would be necessary if the rotor or bucket/... - Page 22 RC3 BP™ Controls, Indicators, and Displays NOTE When changing mode, rpm/RCF values may appear a digit or two off from the original set value. The centrifuge translates set RCF values to rpm whole numbers for speed control purposes, then calculates that rpm whole number to the closest RCF value.
- Page 23 ® Controls, Indicators, and Displays SORVALL Centrifuges During a run, to view in an alternative duration control mode (for example, to monitor accumulating TIME when an ACE run is in progress), press one of the other two duration control keys once – this changes the RUN display to show an accumulating value in that alternative mode without altering the way duration is controlled.
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Page 24: Menu Key And Options
RC3 BP™ Controls, Indicators, and Displays MENU Key and OPTIONS MENU OPTION QC RUN SLOW START SLOW STOP BRAKE OFF STEP RUN The MENU key accesses advanced feature options listed below it, plus other features such as saving parameters to memory. After pressing MENU, pressing 1 accesses a secondary screen to view or enter values, plus confirm option selection;... - Page 25 ® Controls, Indicators, and Displays SORVALL Centrifuges If, during the QC RUN, an observed RUN display temperature is not within ±2°C of SET temperature (as could be the case when there is a significant temperature change with a short run), the centrifuge will continue to repeat that step of the QC RUN sequence until the observed temperature is within range.
- Page 26 RC3 BP™ Controls, Indicators, and Displays most gradual rate, and each successive rate incorporating incrementally more braking up to number 10. Selection of BRAKE OFF will have an affect on SLOW STOP (see the NOTE under BRAKE OFF). • BRAKE OFF deactivates normal deceleration braking for a coasting stop from any specified speed (in rpm) between 6000 and 0.
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Page 27: Numeric Keypad
® Controls, Indicators, and Displays SORVALL Centrifuges • STEP RUN allows the linking-together of up to three sets of programmed run parameters to automatically perform step run protocols. When step run is selected, the specified programs must specify the same rotor at similar temperatures. Step runs can be saved to program memory (program numbers 10-15) for simple recall in future use (see SAVE RUN below). -
Page 28: Start And Stop Keys
RC3 BP™ Controls, Indicators, and Displays START and STOP Keys START STOP The green START key is used to start the centrifuge run. When the START key is pressed, the green LED status indicator light on the key is will blink until the rotor begins to spin (which could take up to 20 seconds if a large rotor is installed and SLOW START is selected). -
Page 29: Main Power And Keypad Lock
® Controls, Indicators, and Displays SORVALL Centrifuges Main POWER and KEYPAD LOCK RUN SINGLE PROGRAM FULL FUNCTION RUN ANY PROGRAM KEYPAD LOCK POWER The Main POWER and KEYPAD LOCK panel is located away from the control console, in the top-right corner of the front cabinet panel. -
Page 30: Set Display Advisory Messages
RC3 BP™ Controls, Indicators, and Displays SET Display Advisory Messages When the RC3BP™ detects a system fault or a situation requiring operator action, an advisory message appears in the SET display and an alarm will sound to alert the operator. In most cases, press- ing CLEAR shuts off the alarm and removes the message from the display (if not, in almost all other cases, turning the main POWER switch OFF and back ON will do so). - Page 31 ® Controls, Indicators, and Displays SORVALL Centrifuges municate to WatchLog Network™ (logging run data will not occur), but the centrifuge should still be operational. If programmed operation is desired, resave the run param- eters, but leave the main power switch ON until the condi- tion is repaired, so that saved programs are not lost.
- Page 32 RC3 BP™ Controls, Indicators, and Displays SAMPLE TEMPERATURE OVER LIMIT During a run, when the pre-cool feature was not active (see SAMPLE OVERTEMP – PRE-COOLING above), the RUN display temperature went above the maximum allowable (overtemperature limit) setting. Check all possible causes: a.
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Page 33: Terminal Conditions
® Controls, Indicators, and Displays SORVALL Centrifuges Terminal Conditions Terminal Condition messages alert the user to a serious fault condition. Runs are disallowed or terminated. These conditions typically cannot be remedied by the user; the centrifuge should be removed from service until it is repaired by Thermo Service. AIR TEMP SENSOR FAILURE The chamber air temperature sensor indicated either an open circuit, or a temperature above 45°C. - Page 34 RC3 BP™ Controls, Indicators, and Displays DRIVE FAULT DETECTED – CALL SERVICE The microcomputer detected either that the drive motor is not responding, or that the tachometer circuit has failed. Wait for the rotor to stop spinning (this could take as long as 20 minutes), then use the mechanical override to remove all sample from the rotor, unplug the centrifuge and contact Thermo Service.
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Page 36: Chapter 4. Operation
During start-up (in addition to lighting all LEDs), the centrifuge microprocessor performs a short diagnostic routine and the SET display will appear as follows: ******** SORVALL RC3BP REV X.XX ******** Self Test in Progress... If an error is detected, an error message will appear in the SET display. -
Page 37: Rotor Installation, Loading And Balancing
® Operation SORVALL Centrifuges Rotor Installation, Loading and Balancing W A R N I N G Install, load, and balance the rotor according to the instructions given in the rotor instruction manual. If maximum compartment mass is exceeded, the maximum speed must be lowered (see Reduc- ing Speed for Rotor Compartment Loads in Excess of Design Mass,... -
Page 38: Normal Operation
RC3 BP™ Operation Normal Operation Read the Safety Information Page at the front of this manual. Check all OPTION LED indicators – if selection changes are required, press MENU, then press ENTER to access each option screen in succession (see figure 4-1). As you step through each screen, you may select or deselect, and change or accept the previous setting. -
Page 39: Programmed Operation
® Operation SORVALL Centrifuges Programmed Operation A. Storing Selections to Program Memory 1. Establish all desired run parameters as explained in steps 1 and 3 of the previous section, Normal Operation. NOTE If you plan on using the QC RUN feature, consider the following as you save runs to program memory: •... -
Page 40: Using Advanced Features (Options)
RC3 BP™ Operation NOTE To prevent accidental erasure of established programs, if the entered program number has been previously assigned, the following message will appear: IN USE - OVERWRITE? (1=YES, O or ENTER=NO) To review stored programs before overwriting, refer to your Program Log Pad or press RECALL. - Page 41 ® Operation SORVALL Centrifuges Advanced feature selection is usually reflected by LED option sta- tus indicators – when a feature has been selected, the corresponding indicator will be lit (although specific settings for several features can only be checked by reselecting the feature in the options screens). If changes to advanced feature selection are required (other than to RPM/RCF or TIME/HOLD/ACE status, which are accessible through primary function keys), the features are accessed by press-...
- Page 42 RC3 BP™ Operation Pressing ENTER steps through the options screen sequence without changing preexisting selection status (unless a new value is input). Selections are made by inputting 1 or 0, then entering any required setting values that may be prompted on a subsequent screen for that option.
- Page 43 ® Operation SORVALL Centrifuges To select the QC RUN feature, press MENU, input 1, install the correct rotor, make sure WatchLog Network™ is running on the connected computer, and press START. The QC RUN sequence will begin, running program number 1, and the run status will be displayed on the WatchLog Network™...
- Page 44 RC3 BP™ Operation SELECT BRAKE OFF? This screen allows you to select a speed (in rpm) at which, after termination, deceleration will transition from braking to a coast- ing stop. The amount of time it takes for the rotor to stop depends on the transition speed, windage and inertia of the rotor.
- Page 45 ® Operation SORVALL Centrifuges SELECT STEP RUN? This screen allows you to link together up to three sets of run parameters from program memory to create a step run. If the desired parameters have not been entered into memory, you must save each desired set of parameters to memory before continuing with step run creation.
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Page 46: Temperature Control
RC3 BP™ Operation Temperature Control The RC3BP™ controls calculated sample temperature during a run based on the rotor selected, rotor speed, run time, SET temperature and measured chamber temperature. The complex calculation the centrifuge performs must assume, however, that the sample and the rotor are at SET temperature at the start of the run. -
Page 47: Running Hazardous Material
® Operation SORVALL Centrifuges 6. Press HOLD to select a continuous run. 7. Press TEMP and enter the desired rotor temperature. 8. Press START to begin rotor temperature equilibration. The cen- trifuge will alert you that a sample overtemperature condition exists, and that pre-cooling is taking place –... -
Page 48: Reducing Speed For Rotor Compartment Loads
RC3 BP™ Operation Reducing Speed for Rotor Compartment Loads in Excess of Design Mass There is a maximum allowable compartment mass established for each centrifuge rotor (see Rotor Information Table on page 4-2). To prevent rotor failure, the total contents of any compartment, includ- ing specimen, tubes or bottles, cover, adapters (if any), and (in many cases) bucket, must not exceed the maximum allowable com- partment mass unless rotor speed is reduced proportionately. -
Page 50: Chapter 5. Maintenance
RC3 BP™ Maintenance Chapter 5: MAINTENANCE This section describes routine maintenance procedures that should be performed on a regular basis. As the user, it is your responsibility to make certain that these procedures are followed when necessary. Also, to keep your centrifuge in good working condition and ensure accurate test results, we recommend that, in addition to these routine procedures, you have the temperature control and the rotor imbalance detector checked periodically by a Thermo Service Representative or other qualified service personnel because occasionally, these controls may need to be recalibrated. -
Page 51: Lubrication
® Maintenance SORVALL Centrifuges Drive Spindle Wipe the spindle with a soft cloth before a rotor is installed to reduce the chance of the rotor sticking to the spindle. Periodi- cally, wash the spindle with warm water. Cabinet Clean the enameled cabinet panels with a household wax cleaner. Use a mild, nonalkaline detergent and water to clean the top deck and the chamber door. -
Page 52: Air Filter Assembly Removal
RC3 BP™ Maintenance CENTRIFUGE BACKPACK BRACKET AIR FILTER ASSEMBLY HANDLE (INCLUDES FILTER AND HOLDER) Figure 5-1. Air Filter Assembly Removal 4. Clean the air filter assembly by rinsing in warm water. If the air filter is being reinstalled proceed to step 6. However, if the air filter can no longer be cleaned by rinsing in warm water or if it is damaged, replace the air filter as described in step 5. -
Page 53: Customer Control Inspection
® Maintenance SORVALL Centrifuges Customer Control Inspection To keep your centrifuge in good working condition and ensure accurate test results, we recommend that you check the speed con- trols, timer controls and temperature controls at least twice a year. If the bi-annual inspection reveals inaccurate results for any of these controls, contact Thermo Service or your local representative for SORVALL ®... - Page 54 RC3 BP™ Maintenance 4. Press START. Using a stopwatch, begin timing precisely as the RUN TIME display counts down to 13:20; then stop timing precisely as the RUN TIME display counts down to 00:00. The stopwatch should read between 13:15 to 13:25, representing 13:20 ±0.5% (4 seconds) plus an additional second to allow for cumulative human error.
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Page 55: Emergency Sample Recovery
® Maintenance SORVALL Centrifuges 7. After termination, immediately after the rotor stops and the door unlocks, open the chamber door and measure the sample temperature using the same calibrated thermometer used earlier. The measured sample temperature should be within 2°C of SET temperature. -
Page 56: Circuit Breakers
RC3 BP™ Maintenance Circuit Breakers The RC3BP™ has seven circuit breakers. The primary power circuit breaker is located on the front cabinet panel (POWER switch). Six circuit breakers are located on the rear console panel (as shown in figure 5-3). The two 20A circuit breakers on the rear console protect the drive circuitry and the refrigeration circuitry and the three 1A and one 3A circuit breakers protect the power supply. - Page 57 ® Maintenance SORVALL Centrifuges ® The SORVALL Product Guide contains descriptions of com- monly used decontamination methods and a chart showing method compatibility with various materials. The Care and Maintenance Section of the centrifuge or rotor instruction manual contains specific guidance about cleaning and decontamination methods appropriate for the product it describes.
- Page 58 RC3 BP™ Maintenance If a centrifuge or rotor to be serviced does not have a Decontamina- tion Information Certificate attached and, in Thermo's opinion presents a potential radioactive or biological hazard, the Thermo representative will not service the equipment until proper decon- tamination and certification is complete.
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Page 60: Appendix
RC3 BP™ Appendix APPENDIX... -
Page 61: Warranty
® Appendix SORVALL Centrifuges Warranty Thermo Electron Corporation makes no warranty of any kind, expressed or implied, except as stated in this warranty policy. ® The SORVALL RC3BP™ Centrifuge is warranted (subject to the conditions specified below and in the warranty clause of the Thermo... - Page 62 DECONTAMINATION INFORMATION CERTIFICATE Complete and attach to equipment BEFORE servicing (instructions on reverse) PLEASE PRINT DECONTAMINATION CERTIFIED BY _____________________________________________________ TITLE/POSITION ___________________________________________________ PHONE ___________________________ FAX ____________________________ DEPARTMENT _________________________________________________ INSTITUTION _____________________________________________ ADDRESS _________________________________________________________________ CITY _____________________________________________ STATE ____________________________________ ZIP _________________________________ INSTRUMENT ____________________________________________________________________ SERIAL NUMBER _____________________________________ ROTOR _________________________________________________________________________ SERIAL NUMBER _____________________________________ PART ______________________________________________________________________________________...
- Page 63 INSTRUCTIONS When an instrument that has been used with radioactive, patho- and certification has been completed. If an instrument is received at genic, or otherwise hazardous materials requires servicing by Thermo our Service facilities and, in our opinion, poses a radioactive or personnel either at the customer's laboratory or at Thermo facilities, biological hazard, the sender will be contacted for instructions as to the following procedure must be complied with to insure safety of...
- Page 64 CHINA JAPAN INDIA Tel.: 86 21 6865 4588 Tel.: 81 45 453 9220 Tel.: 91 22 5542 9494 86 10 5850 3588 OTHER ASIA PACIFIC COUNTRIES Tel.: 852 2885 4613 BELGIUM AUSTRIA FINLAND Tel.: 32 2 482 30 30 Tel.: 43 (1) 801 40 0 Tel.: 358 9 329 100 FRANCE GERMANY...
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