Thermo Electron C3i User Manual
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Thermo Electron C3i User Manual

Jouan 4 x 280 ml multifunction centrifuges
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C3i - CR3i
Jouan 4 x 280 mL Multifunction Centrifuges
User's Manual
Manual N°: 89003152-a

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Summary of Contents for Thermo Electron C3i

  • Page 1 C3i - CR3i Jouan 4 x 280 mL Multifunction Centrifuges User’s Manual Manual N°: 89003152-a...
  • Page 2 CAREFULLY READ THIS MANUAL BEFORE OPERATING YOUR INSTRUMENT. INFORMATION CONTAINED IN THIS DOCUMENT IS THE PROPERTY OF THERMO ELECTRON CORPORATION; IT MAY NOT BE DUPLICATED OR DISTRIBUTED WITHOUT THE OWNER’S AUTHORIZATION. THE VALIDITY OF THE GUARANTEE IS SUBJECT TO THE OBSERVATION OF THE INSTRUCTIONS...
  • Page 3 C3i-CR3i Revision Status Revision Status DATE AMENDED PAGES NOTES 07/04 Initial release...
  • Page 4 Rotor locking nut Toolbox : 26792017 Rotor locking washer - Rotor removal tool 86004061 Rotor locking wrench - Emergency unlocking tool 86003941 Fuse 5X20 3.15A T 250V - Spare fuses (C3i only) For missing parts call your nearest Thermo representative...
  • Page 5 C3i-CR3i Guarantee Terms Guarantee Terms JOUAN guarantees that this unit is free from defects in materials and workmanship when it leaves the factory, and will replace or repair the unit if it proves defective in normal use or during service for a period of ONE YEAR from the delivery.

  • Page 6: Table Of Contents

    C3i-CR3i Contents Contents Use and Function ..............

  • Page 7: Use And Function

    : if unobserved the result could be harm to the operator. 1.3. Description The C3i and CR3i centrifuges are designed for laboratory use. They will separate the components of fluids into layers of varying density by subjecting them to high forces.

  • Page 8: Refrigeration And Heating Systems

    Use and Function 1.4. Refrigeration and Heating Systems The C3i is a ventilated unit. A permanent air circulation reduces the heat level in the bowl. However the temperature in the bowl can exceed 10 °C above the ambient temperature after hours of continuous operation.

  • Page 9: Imbalance Detection System

    Bypass the interlock system only under emergency conditions as the rotor could still be rotating. 1.7. Imbalance Detection System The C3i centrifuge is equipped with a load imbalance detector. In case of excessive imbalance, the load imbalance indicator LED is illuminated and the brake is applied immediately.
  • Page 10 C3i-CR3i Use and Function Centrifugation formulae Legend : R = radius (in millimetres) N = speed (in r.p.m.) ÷ 1000 RCF = gravitational acceleration ‘g’ M+ = add to memory MR = memory recall NOTE : To calculate actual results, press the keys on a pocket calculator in the order shown.

  • Page 11: Specifications

    Specifications 2.1. Dimensions and Weight Dimensions (H x W x D) C3i : 372 x 400 x 502 mm CR3i : 375 x 575 x 605 mm Packed (H x W x D) C3i : 600 x 610 x 540 mm...
  • Page 12 C3i-CR3i Specifications...

  • Page 13: Installation

    C3i-CR3i Installation Installation 3.1. Environmental Conditions General conditions accepted for centrifuge transport and storage are: - Ambient temperature -20°C to +50°C. - Relative humidity up to 90%. General conditions accepted for operating the centrifuge safely are: - Indoor use. - Temperature: 5 °C to 40 °C.

  • Page 14: Mains Supply

    C3i-CR3i Installation 3.4. Mains Supply Check mains and frequency: they must correspond to the values shown on the instrument identification label. Cat. No. Content Voltage Frequency Type 11175770 230V ± 10% 50/60 Hz 10 A single phase + ground 11175774...

  • Page 15: Manual Lid Unlocking Procedure

    C3i-CR3i Installation 3.6. Manual Lid Unlocking Procedure In the event of mains non-availability or power failure, opening of the lid is prevented by the lid locking safety device. It is recommended that the user waits for the mains to be switched back on so that this safety device enables the lid to be unlocked.

  • Page 16: Performance And Accessories

    10 x 42 4100 2988 11175738 Set of 4 inserts 4 x 15 ml vacu* 16 x 124 4100 3063 Note : the RCF applied to tubes in T40 inserts is about 5% lower for the C3i. * In open buckets only...
  • Page 17 Rubber cushion for flexible microtitre plates 11175755 AC 15.4 Angle rotor 30 x 15 ml 17.5 x 100 C3i : 4000 C3i : 2415 CR3i : 4100 CR3i : 2537 11175756 AC 100.10 Angle rotor 6 x 100 ml 38 x 101...
  • Page 18 C3i-CR3i Installation...

  • Page 19: Instructions For Use

    C3i-CR3i Instructions for Use Instructions for Use 4.1. Controls and Indicators All the controls are located on the front panel. The front panel is an intuitive interface: no previous operational knowledge is necessary; every parameter can be set by pressing the cursor key continuously, from the minimum to the maximum and viceversa.

  • Page 20: Display Screens

    Lower screen This screen displays either the acceleration or the deceleration profile selected (17) on the C3i for the given program. The value of the acceleration profile is displayed either during acceleration or when the acceleration set button (8) is pressed. The value of the deceleration profile is displayed either during deceleration or when the deceleration set button (9) is pressed.

  • Page 21: Control Panel Indicators

    C3i-CR3i Instructions for Use Function Keystroke sequence and description Set centrifugal When the LED next to ‘RADIUS’ (21) is illuminated, the middle display radius shows the value of the centrifugal radius in millimeters. To increase/decrease the centrifugal radius, press on the corresponding up/down arrows. This parameter can only be viewed and set while the centrifuge is at rest.

  • Page 22: Description Of Certain Events With Respect To The Control System

    C3i-CR3i Instructions for Use 4.5. Description of Certain Events with Respect to the Control System Start and acceleration phase When the start button is pressed, the centrifuge will begin to accelerate the rotor. At this point the run timer will start and the middle display will begin to show remaining run time. The upper display will show either actual speed or actual maximum RCF values, whichever is currently selected.

  • Page 23: Sample Loading

    C3i-CR3i Instructions for Use The centrifuge also should be observed to verify proper appearance of screws, lid hooks, latches and for evidence of corrosion. In case of problems contact your Thermo Service representative, because any deviation from the above mentioned advice may have serious consequences for the safety of the appliance and of the operators.
  • Page 24 C3i-CR3i Instructions for Use INCORRECT DYNAMIC BALANCING : CORRECT If less than the maximum complement of samples is loaded, the tubes must be placed in opposite pockets. An odd number of tubes requires an additional blank, water-filled tube of identical total weight to balance.

  • Page 25: Hazards, Precautions And Limitations Of Use

    C3i-CR3i Hazards, Precautions and Limitations of Use Hazards, Precautions and Limitations of Use 5.1. IEC 1010-2-020 This centrifuge conforms to the IEC 1010-2-020 international standard, which specifies particular safety requirements for laboratory centrifuges. According to the standard warnings, the operator must respect the following precautions : - Mark a boundary area of 30 cm around the centrifuge.

  • Page 26: Operational Limitations

    Hazards, Precautions and Limitations of Use 5.4. Operational Limitations The C3i centrifuge and its associated rotors offer a high level of RCF performance. In some cases the maximum possible RCF will exceed the one sustainable by the sample container. The rotor RCF performance displayed is the maximum available in the rotor pockets. Depending on the sample containers or on other application needs, the maximum RCF which must be used is to be determined by the operator.

  • Page 27: Service And Maintenance

    C3i-CR3i Service and Maintenance Service and Maintenance All cleaning operations should be performed with the centrifuge disconnected from the power outlet. 6.1. Periodic Cleaning Daily No daily cleaning is required, except in the case of accidental tube breakage, or when there is a large amount of spillage in the bowl.

  • Page 28: Radioactive Decontamination

    C3i-CR3i Service and Maintenance Any part which has been subjected to temperatures above 130 °C must be discarded. Hypochlorite “bleach” used at 0.1% concentration with 10 minutes immersion is effective against bacteria, spores and viruses but, as an oxidizing agent, is corrosive to metal alloys and must be thoroughly rinsed off of metal parts and the parts dried.

  • Page 29: Trunnion Lubrication

    C3i-CR3i Service and Maintenance 6.7. Trunnion Lubrication This operation is necessary to allow the buckets to swing freely. Clean the trunnions regularly with a dry wipe (as well as the part of the bucket that rotates on the trunnions). Then, put a very small quantity of grease on the curved face of the trunnion.

  • Page 30: Chemical Compatibility Table For Rotors, Tubes And Accessories

    C3i-CR3i Service and Maintenance 6.8. Chemical Compatibility Table for Rotors, Tubes and Accessories S = Satisfactory = Aluminum = Buna N D = Discoloration but OK = Cellulose acetate butyrate = Cellulose Nitrate P = Pure chemical OK = Delrin...
  • Page 31 C3i-CR3i Service and Maintenance Material AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA VX Chemical PPCO Bromoform Butadiene Butane n-Butanol n-Butyl Acetate Butylene Butyl Chloride Butyric Acid U Mt U...
  • Page 32 C3i-CR3i Service and Maintenance Material AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA VX Chemical PPCO Diphenyloxide Dipropylene Glycol Distilled Water Ethanol (50%) Ethanol (95%) Ethyl Acetate U M U...
  • Page 33 C3i-CR3i Service and Maintenance Material AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA VX Chemical PPCO Mercury Methanol (100%) Methyl Acetate O M U M M U Methyl Alcohol (100%)
  • Page 34 C3i-CR3i Service and Maintenance Material AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA VX Chemical PPCO Rubidium Bromide Saccharose Salycylic Acid, Sat Serum Silver Acetate Silver Nitrate Sodium Acetate Sodium Bisulphate...

  • Page 35: Disposal Of Product

    C3i-CR3i Disposal of Product Disposal of Product The following disposal recommendations are valid for the machine (or its accessories) at the end of its useful life, or the parts that must be disposed of because they are worn out or degraded during use.
  • Page 36 C3i-CR3i Certificate of Decontamination and Cleaning Certificate of Decontamination and Cleaning Read carefully the instructions below before sending an instrument, or parts of it, to Thermo Service Dept. (Jouan S.A.) or to any Authorized Technical Assistance Service Mr / Mrs (name) Establishment ..................

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Cr3i

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