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USER MANUAL
DU-8200 Series
UV Visible Spectrophotometers
Drawell International Technology Limited
Chongqing Drawell Instrument CO,.Ltd
Add:Suite 2705,Building No.12,Shiyou Road No.1,Yuzhong District, Chongqing,China
Tel: 0086-023-63268643
Shanghai Drawell Scientific Instrument Co.,Ltd
Add : Suite 1117,Lane561XiuChuan Rd.,PuDong New Area,Shanghai,China
Web : www.drawell.com.cn
Email : sales05@drawell.com.cn
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Summary of Contents for Drawell DU-8200 Series
- Page 1 DU-8200 Series UV Visible Spectrophotometers Drawell International Technology Limited Chongqing Drawell Instrument CO,.Ltd Add:Suite 2705,Building No.12,Shiyou Road No.1,Yuzhong District, Chongqing,China Tel: 0086-023-63268643 Shanghai Drawell Scientific Instrument Co.,Ltd Add : Suite 1117,Lane561XiuChuan Rd.,PuDong New Area,Shanghai,China Web : www.drawell.com.cn Email : sales05@drawell.com.cn...
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Page 2: Table Of Contents
Contents Chapter 1 Preface……………………………………………………………………………1 1. Safety………………………………………………………………………………1 2. General……………………………………………………………………………………1 3. Electrical … ………………………………………………………………………………1 4. Warning…………………………………………………………………………………………………….. 1 5. Performance……………………………………………………………………………………………….. 2 6. Radio Interference ………………………………………………………………………………………2 Chapter 2 Introduction of the Instrument............3 1. Brief Introduction of the instrument..............3 2. Working Principle………………………………………………………………………4 3. Structure...................4 4. Unpacking Instructions………………………………………………………………………………….5 5. - Page 3 Power On/Off D2 Lamp………………………………………………………18 Power On/Off W Lamp………………………………………………………18 Set Date and time……………………………………………………………19 Calibrate Dark Current………………………………………………………19 Wavelength Calibration………………………………………………………20 Input the lamps changing Wavelength Point………………………………20 Reload Default…………………………………………………………………21 Version…………………………………………………………………………21 Chapter 5 Instrument Maintenance……………………………………………………22 1. Daily Maintenance……………………………………………………………………………………….22 2. Trouble shooting………………………………………………………………………………………….22 3. Sparepart Replacement………………………………………………………………………………..23...
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Page 4: Chapter 1 Preface
CHAPTER 1 PREFACE 1, Safety The safety statements in this manual comply with the requirements of the HEALTH AND SAFETY AT WORK ACT, 1974. Read the following before installing and using the instrument and its accessories. This instrument should be operated by appropriate laboratory technicians. 2, General The apparatus described in this manual is designed to be used by properly trained personnel in a suitable equipped laboratory. -
Page 5: Performance
▲Shows visible damage ▲Fails to perform the intended measurements ▲Has been subjected to prolonged storage under unfavorable conditions ▲Has been subjected to severe transport stresses 5, Performance To ensure that the instrument is working within its specification, especially when making measurements of an important nature，carry out performance checks with particular reference to wavelength and absorbance accuracy. -
Page 6: Chapter 2 Introduction Of The Instrument
CHAPTER 2 INTRODUCTION OF THE INSTRUMENT 1. Brief Introduction of the Instrument This instrument is a single beam, general purpose instrument designed to meet the needs of the Conventional Laboratory, This instrument is ideal for various applications, such as: Chemistry, Biochemistry, Petro-chemistry, Environmental Protection, Food and Beverage Labs, Water and Waste Water Labs and other fields of quality control and research. -
Page 7: Working Principle
2. Working Principle Different matter has different but special absorbance wavelength point. Also, when at the fixed wavelength point, the absorbance has some relation to the substance’s (Always transparent Solution) concentration and its thickness. The relation can be concluded as the following Formula which is called Lambert-Beer Law. T= I/I A=KCL= -log I/I Absorbance... -
Page 8: Unpacking Instructions
4. Unpacking Instructions Carefully unpack the contents and check the materials against the following packing list to ensure that you have received everything in good condition. Packing List Description Quantity Spectrophotometer ............... …….… Power Cord ................…….… 1cm Glass Cuvettes………………………………………..…………… 1 Set of 4 1cm Quartz Cuvettes………………………………………...…………. -
Page 9: Chapter 3 Install Instrument
CHAPTER 3 INSTALL INSTRUMENT 1. Environment Required To ensure the best performance, the following conditions are required: The best working temperature range is 16-30℃ and the humidity is 45-80%. Keep it as far as possible away from the strong magnetic or electrical fields or any electrical device that may generate high-frequency fields. -
Page 10: Chapter 4 Operation Introduction
CHAPTER 4 OPERATION INTRODUCTION 1. Software Structure 1.1 Menu Tree Main Basic Mode Absorbance Menu Transmittance Energy Measurement Measure Print Quantitative Measure Standard Curve Measurement Set Wavelength Print Set Parameters Conc. Unit Qty. of Standards Conc. Of Standards Display the Curve Select Curve Coefficient Choose Equation... -
Page 11: Basic Operation
Coefficient Method: Input the values of the curve equation’s coefficient, and then measure the unknown sample’s concentration. 3) Setup Light source management; Get Dark Current; Calibrate Wavelength and Set time, etc. 2. Basic Operation 2.1 Choose Photometric Mode Basi c Mode ENTER Basi c Mode Quanti t ati v e... -
Page 12: Print The Test Result
Basi c Mode ENTER Basi c Mode Quanti t ati v e 0.000 Abs Put the samples in the light path and press to measure. Setup 08:08 500.0 nm 08/26 2.9 Print the test result Basi c Mode ENTER Basi c Mode Quanti t ati v e 0.000 Abs In the Measurement Interface, press... - Page 13 Basi c Mode Basi c Mode GOTOλ WL=500.0nm 0.000 Abs Press Pl e ase Input WL. to set Wavelength，input the wavelength value by the Arrow 500.0 nm Basi c Mode Basi c Mode GOTOλ WL=500.0nm 0.000 Abs Pl e ase Input WL. Keypad, then press to confirm.（Fig.
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Page 14: Measurement
Step 5, Calibrate 100%T/0Abs Put the Reference Solution in the light path，press ZERO to calibrate 100%T/0Abs； Step 6, Measure Sample START STOP Put the unknown concentration sample in the light path, press measure， and the result displays in the data sheet. At the same time, the test result will be stored in the RAM automatically. - Page 15 1. % 5. ml/l ENTER √Quantitative 2. ug/l 6. mg/ml Standard Curve 3. mg/l 7. ug/ml Coefficient 4. g/l 8. None Unit Fig. 4-7 Step 3: Choose Methods There are two methods to be selected: Standard Curve and Coefficient. I. Standard Curve： (1) Go into “Standard Curve”Interface In “Quantitative”...
- Page 16 500.0nm 0.000Abs Quantitative GOTOλ WL=500.0nm Input Stds No: Please Input WL. No.= 3 Fig. 4-10 ★ Use the Arrow Key to input the quantity of the standard samples, 500.0nm 0.000Abs 500.0nm 0.000Abs Put 1# Standard, Quanti t ati v e: ENTER then press Input 1# Conc.:...
- Page 17 same time, the Curve Equation will be saved in the RAM automatically. 0.600 500.0nm 0.600Abs Put 6# Standard, ENTER Input 6# Conc.: 0.000 0.000 0.600 r=1.000 C=1.000XA+0.000 Fig. 4-13 Note: If there’s some mistake during the operation, the system will beep 3 times and return the initial interface automatically.
- Page 18 Curve Data 28.00 ENTER 1 C=1.852A+2.010 2 C=1.000A+0.000 0.000 ★ Move the cursor on the equation that you need and press 0.000 0.800 r=1.000 C=1.852A+2.010 confirm. Then the corresponding curve will display on the screen. (Fig. 4-16) 28.00 Curve Data ENTER 1 C=1.852A+2.010 2 C=1.000A+0.000...
- Page 19 II Coefficient Method： (1) Go into “Coefficient” method. √Quant i t a t i v e Coef f i c i e nt Coef f i c i e nt ENTER St a ndar d Cur v e 0. 0 00 Abs Coef f i c i e nt K Press Arrow Key to choose “Coefficient”, press to go into...
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Page 20: Setup
Coefficient Quantitative GOTOλ 0.000 Abs WL=500.0nm 0.000 mg/ml Please Input WL. 500.0 nm Fig. 4-21 (4) Calibrate 100%T/0Abs START Coeffi c i e nt 500.0nm 0.000Abs STOP 0.000 Abs Abs. Mg/ml Press to go into continuous measurement interface (Fig. 0.000 mg/ml 500.0 nm 4-22). -
Page 21: Power On/Off D2 Lamp
ENTER √Setup Basic Mode D2 Lamp Quantitative W Lamp Setup 08:08 Date & Time 08/16 Fig. 4-23 1) Power on/off D2 lamp When the wavelength point needed is in the range of 340-1100nm，the D2 lamp can be switched off to prolong its life time. -
Page 22: Set Date And Time
ENTER √Setup √W Lamp D2 Lamp √ W Lamp Date & Time Fig. 4-25 3) Set Date & Time ENTER √Setup Date & Ti m e Ti m e 12: 00: 00 D2 Lamp On Press Arrow Key to choose “Date & Time” and press to go into Date 2008/08/08 W Lamp On... -
Page 23: Wavelength Calibration
5) Wavelength Calibration After some time, the energy of lights would drop off and there will be some but slight influence on the test result. In this case, users can reset the wavelength to compensate. We suggest the users to reset the wavelength every one or two months. Remove all the blocks in the light path and close the lid of the compartment, √Setup ENTER... -
Page 24: Reload Default
7) Load Default ENTER √Setup √Load Defaul t Reset WL. Press Arrow Key to choose “Load Default” and press to confirm.（Fig. Lamp Change Load Defaul t ENTER √Setup √Load Defaul t Reset WL. 4-30） ， press Arrow Key to choose “Yes” and press to confirm. -
Page 25: Chapter 5 Instrument Maintenance
CHAPTER 5 Instrument Maintenance To keep the instrument work in good condition, constant maintain is needed. 1. Daily Maintain 1) Check the compartment After measurement, the cuvettes with sample solutions should be taken out of the compartment in time. Or the volatilization of the solution would make the mirror go moldy. -
Page 26: Sparepart Replacement
Glass cuvettes used in UV Range Use Silicon Cuvettes. No stable Sample Improve the sample Much higher sample concentration Dilute the sample Low voltage or unstable power supply Improve the power condition Lights defect Replace a new lamp ... - Page 27 Fig. 5-2 Step 5: Reset the fuse seat Replace the fuse seat in the power socket Step 6: Switch on the power Plug the socket and switch on the power - 24 -...
- Page 28 Drawell International Technology Limited Chongqing Drawell Instrument CO,.Ltd Add:Suite 2705,Building No.12,Shiyou Road No.1,Yuzhong District, Chongqing,China Tel: 0086-023-63268643 Shanghai Drawell Scientific Instrument Co.,Ltd Add : Suite 1117,Lane561 XiuChuan Rd.,PuDong New Area,Shanghai,China Web : www.drawell.com.cn Email : sales05@drawell.com.cn...
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