Drawell DU-8800DS Series User Manual
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Drawell DU-8800DS Series User Manual

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USER'S MANUAL
For DU-8800DS/RS Series
Spectrophotometers
Drawell Scientific

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  • Page 1 USER’S MANUAL For DU-8800DS/RS Series Spectrophotometers Drawell Scientific...

  • Page 3: Table Of Contents

    Contents Safety ……..……………………………………………………………………. General ………………………………………………………………………. Electrical …………………………………………………………………….. Warning ………………………………………………………………………….. Performance ………………………………………………………………… Radio Interference ………………………………………………………….. Introduction ………………………………………………………………… Working Principle ………………………………………………………….. Unpacking Instructions ……………………………………………………. Specifications ……………………………………………..………………… 4 Installation ……………………………………………………………………… .4 Operation ………………………………………………………..……….…………5 Prepare the Spectrophotometer …………………………………………… 5 ………… ……………………………………… Description of keys Turn on spectrophotometer …...

  • Page 5: Safety

    Safety: The safety statements in this manual comply with the requirements of the HEALTH AND SAFETY AT WORK ACT, 1974. Read the following before installing and using the instrument and its accessories. This instrument should be operated by appropriate laboratory technicians.

  • Page 6: Performance

    Has been subjected to prolonged storage under unfavorable conditions   Has been subjected to severe transport stresses Performance: To ensure that the instrument is working within its specification, especially when making measurements of an important nature，carry out performance checks with particular reference to wavelength and absorbance accuracy. Performance checks are detailed in this manual.

  • Page 7: Working Principle

    Power switch Fuse Power socket USB Port Parallel Port Fig1 Working Principle: The spectrophotometer consists of five parts: 1) Halogen or deuterium lamps to supply the light; 2) A Monochromator to isolate the wavelength of interest and eliminate the unwanted second order radiation; 3) A sample compartment to accommodate the sample solution;...

  • Page 8: Unpacking Instructions

    Unpacking Instructions: Carefully unpack the contents and check the materials against the following packing list to ensure that you have received everything in good condition. Packing List Description Quantity Spectrophotometer............ Mains Lead.............. Glass Cuvettes………………………………………… 1 Set of 4 ...

  • Page 9: Operation

    4. Use the appropriate power cord and plug into a grounded outlet. 5. Turn on your spectrophotometer. Allow it to warm up for 15 minutes before taking any readings. We suggest you then do the Calibrate System with the Search 656.1nm to set the wavelength to the deuterium lamp emission line.

  • Page 10: Turn On Spectrophotometer

    Save data or curve; 【SAVE】 Set wavelength; 【SETλ】 Blank or scan the user base line; 【ZERO】 Print test results or screen 【PRINT】 Start testing or scanning sample; 【START】 Exit to previous screen or cancel the operation; 【ESC/STOP】 Confirm the inputted data or selected item; Go into next 【ENTER】...

  • Page 11: Basic Operation

    Fig 4A Fig 5 Fig 6 Fig 7  Basic operation ※ Blank There is a system baseline stored in the memory of the instrument. Usually user may not rebuild system baseline before test. Only putting the sample into the sample light path and the reference into the Reference Light Path, the result can be obtained.
  • Page 12 the instrument is powered on, it is necessary for the user to rebuild the system baseline. There are a couple of ways to rebuild the system baseline. Select “Yes” in Fig 5 or Press【0】In Fig 73 or Press【F4】In Fig41, Regarding Blanking, important points list below: A.
  • Page 13 automatically. However the 【START】must be pressed in other measurements such as DNA/Protein, Muli WL and Quantitative etc. B. Take measure in WL Scan a. After all scan parameters are entered, put the reference cuvette with reference solution into the Reference Light Path and the sample cuvette with sample solution into the sample light path,Press 【START】to scan b.
  • Page 14 Fig 12 ※ Load or delete data or curve (Take the “WL scan” test For example) Press 【3】 in Fig.7 go into “WL scan”. After【LOAD】being pressed, the first file (ABC.wav)in memory will appear on the bottom line of screen .Showed as Fig 13. Press 【∧】or【∨】 to browse the files stroed in memory.
  • Page 15 Table 1 Test File Type Quantitative Curve ***.fit Quantitative Test Result ***.qua WL Scan ***.wav Kinetics ***.kin DNA/Protein ***.dna Multi WL ***.mul WL Validity Accu. Validity ※ Save data or curve (Example: Save curve in “WL scan”)  Press the key 【SAVE】 in Fig14 to save curve. ...

  • Page 16: Analyse Sample

    Fig 16 Fig 17 Before measurement Make a blank reference solution by filling a clean cuvette (or test tube) half  full with distilled or de-ionized water or other specified solvent. Wipe the cuvette with tissue to remove the fingerprints and droplets of liquid. Analyze Sample For different user’s requirements, we provide different test methods.
  • Page 17 Fig19 Test There are three modes (T%, Abs, conc/factor) for you to select by pressing 【F2】to make choice. Fig 20 1. Abs mode Push the blank cuvette into the Reference Light Path and Main Light Path. Press 【F2】 to select Abs mode ,Press 【ZERO】for Blanking , and then Push the sample into Main Light Path to take reading(Fig 20) 2.
  • Page 18 Fig 22 Push the blank cuvette into the Reference Light Path and Main Light Path and press 【ZERO】for Blanking. There are now two choices for you to take: 4.1 Press【F3】to input known F value, Fig 23. Then push the sample into Main Light Path to take reading of concentration 4.2 Push sample of known concentration into the Main Light Path Press【F4】to input known Conc value, Fig 24.

  • Page 19: Quantitative

    Print Test Report Press 【PRINT】to print test results (Fig 25). Fig 25 Quantitative Press【2】in Main Menu for “Quantitative” Test (Fig 26). Press【ESC/STOP】 to exit. Note: .If no automatic changer installed “cell #1” will disappear in Fig26. Fig 26 How to operate 1.
  • Page 20 Fig 28 3. Press 【F2】in Fig 26 for more items to select .See Fig 29. Fig 29 3.1 Press 【F1】in Fig 29 to select fitting method. There are 4 methods for you to choose: Linear fit, linear fit through zero, square fit and cubic fit.
  • Page 21 3.3 Press 【F3】in Fig 29 to establish a standard curve by measuring a group of standard samples. See Fig 30. 3.3.1 Enter standard concentrations of samples by pressing the Numeric keypad followed by【ENTER】. Press 【∧】 or【∨】 to modify the inputted data (Fig31). Press【ESC/STOP】to finish inputting and to exit (Fig 32).
  • Page 22 3.3.4 Press【F4】to draw the curve. You can get a different curve by pressing 【F1】to select a different fitting method. (See Fig 34-Fig37.) For linear fits, “ r ” represent fitting coefficient of linear regression .r=1 is best fitting.usually “ r ” is very close to 1. Note:If there are few standard samples, it is not suitable for selecting square fitting, especially cubic fitting, otherwise invalid fitting result will be obtained.
  • Page 23 Fig 36 cubic fit Fig 37 linear fit 3.3.5 Press 【SAVE】 to save calibration if required 3.3.6 Press【ESC/STOP】to exit 4. Quantitative Test Before test,the standard curve must be obtained.There are three ways for you to obtained it (a, b or c). Standard curve built up and saved in the instrument.

  • Page 24: Wl Scan

    Fig 38 4.3 If there is more than one sample, repeat step 4.2 for the next sample 4.4 Press (SAVE) to save the results and fitting parameters Print Test Report Press the key【PRINT】to print the test report (Fig 39). Fig 40 WL Scan Press【3】in main menu for “WL Scan”...
  • Page 25 Scan sample 1. Press 【F1】 to setup, input the start wavelength, and end wavelength by pressing the numeric keypad (Fig 42). Note: This instrument scans from high to low wavelength. Browse and select the items of scan step and scan speed by pressing 【∧】or【∨】. Fig 42 Note: “Scan step”...
  • Page 26 4. Put the sample cuvette into Main Light Path, press 【START】to scan the sample(Fig 45) 【ESC/STOP】 to stop scanning. When scan has finished the beeper beeps 3 times (Fig 46). Fig 45 Fig 46 5. If you want to change the scale, press 【＜】 or 【＞】 to change “x” scale (Fig 47), input upper limit and lower limit by pressing the numeric keypad .
  • Page 27 Fig 48 6. Press 【F3】 to search the Abs/%T value of the scan. There are two ways for you to search (Fig 49). Fig 49 1) Peak to peak, press 【 F1 】 to set “peak height” and input value by pressing the numeric keypad (Fig 50).
  • Page 28 Fig 51 2) Point to point, Press 【＞】 to search the point from left to right and press 【＜) to search from right to left. The search step interval is the same as the scan step. The value of every point searched will be displayed on the screen.

  • Page 29: Kinetics

    Kinetics Press【4】in main menu for “Kinetics” (Fig 53). 【ESC/STOP】to exit. To load a previous kinetics result, press 【 LOAD 】 and select a previously stored result (.kin) Fig 53 Test 1. Press 【F1】 to set “Total Time”, ”Delay Time”, ”Time interval”, and input the value by pressing the numeric keypad (Fig 54).
  • Page 30 Fig 56 5. Press 【F3】to process the data, and enter “Begin Time”, ”End Time” and ”Factor” (Fig 57) and the value in I.U. will be calculated and displayed (Fig 58). The average straight line between the Begin Time and End Time will be calculated. The gradient of this line gives the rate of change of ΔA/min.
  • Page 31 Save Curve Press the key 【 SAVE 】 to save curve. Note: Load/Save requires the first kinetics display page Fig. 56. Press ESC if in Search to return to the required page. Print Test Report Press the key【PRINT】 to print the curve you have loaded or scanned (Fig 59). Fig 59...

  • Page 32: Dna/Protein

    DNA/Protein Press【5】in main menu for “DNA/Protein” (Fig 60). 【ESC/STOP】to exit. Note:The algorithm of the test refer to Appendix A please. Fig 60 To load previous DNA results, press (LOAD) and select a previously stored result (.dna) Test 1. To use a simpler or different algorithm, you can enter your own values for f1-f4.
  • Page 33 Fig 63 3. Press 【F3】to select the unit of concentration (Fig 64). Fig 64 4. Push the blank cuvette into the Reference Light Path and Main Light Path , then press 【ZERO】for blanking . 5. Pull the sample cuvette into Main Light Path, press 【START】 to test the sample.

  • Page 34: Multi Wavelength

    Fig 66 Recall the default Press the key【F4】to recall the default of the f1-f4. Save Data Press the key 【SAVE】 to save data. Print Test Report Press the key【PRINT】to print the test result (Fig 67). Fig 67 Multi Wavelength Press【6】in main menu for “Multi WL” (Fig 68). 【ESC/STOP】to exit. Fig 68 To load previous Multi Wavelength results, press (LOAD) and select previously stored results (.mul)
  • Page 35 Test 1. Press 【F1】 to setup a group of wavelengths for testing by pressing the numeric keypad followed by 【 ENTER 】 . ( ) or ( ) to modify the inputted data Fig. 69. Press【ESC/STOP】to finish setup and exit. Note: It is recommended to enter the highest wavelength first.
  • Page 36 Fig 71 5. If there is more than one sample, repeat step 4 for the next sample. Note: When the test has finished, the wavelength will go to the first 6. Press 【＜)or【＞】for searching. Input the sample number, the result will be displayed on the screen.

  • Page 37: Setting And Calibration

    Setting and Calibration Utility Press【7】in Main menu for “Utility” (Fig 73). 【ESC/STOP】to exit. Fig 73 WL Reset Press【1】to reset wavelength (Fig74). Fig 74 Printer Press【2】to set printer (Fig 75). 【ESC/STOP】to exit. Fig 75...
  • Page 38 Press【1】in Fig 75 to Reset Printer. Press【2】in Fig 75 to select print port (LPT or Comm., Fig 76). Fig 76 Press【3】 in Fig 75 to select printer (HP PCL (1 colour cartridge), PCL (black mode), Epson ESC/P or Epson/P2 or above, Fig77). Fig 77 Press 【4】...
  • Page 39 Lamp Press【3】to set lamp (Fig 79). 【ESC/STOP】to exit. Fig 79 1. Press【1】in Fig 79 to switch on/off D2. Fig 80. Fig 80 2. Press【2】in Fig 79 to reset usage time of D2(Fig 81). Press 【∧】or【∨】 to select “Yes” or “No”, and then press 【ENTER】. Fig 81 3.
  • Page 40 Fig 82 4. Press 【4】in Fig 79 to reset usage of W (Fig 83). Press 【∧】or【∨】 to select “Yes” or “No”, and then press 【ENTER】. Fig 83 5. Press【5】 in Fig 79 to set the switch usage point of D2 and W lamp (Fig 84).
  • Page 41 Fig 85 1. Press 【1】in Fig 85 to modify time by pressing the numeric keypad (Fig 86). Fig 86 2. Press 【2】in Fig 85 to modify date by pressing the numeric keypad. 3. Press【3】in Fig 85 to set the date display on the top right corner of the screen.
  • Page 42 Dark Current Press 【5】In Fig73 to get dark current (Fig 88). Fig 88 Accu Validity Press 【4】In Fig73 to do Accu Validity (Fig 89). 【ESC/STOP】to exit. Fig 89 Press 【SETλ】 to set the wavelength. Press 【ENTER】to edit and input wavelength by pressing the numeric keypad (Fig 90). 【ESC/STOP】 to finish inputting and exit.
  • Page 43 Fig 91 Press 【F2】to select test mode (Abs or %T, Fig 92). Fig 92 Press 【 F3 】 to set tolerance (Fig 93).Input the value by pressing the numeric keypad. Fig 93 Press【ZERO】for Blanking. Put the sample (calibrated neutral density filter) into Main Light Path. Press 【START】to check.
  • Page 44 Fig 94 WL Validity Press【7】in Fig 73 to WL validity (Fig 95). 【ESC/STOP】to exit. Fig 95 2. Press 【F1】to set the standard peak. Press 【ENTER】to edit and input wavelength pressing numeric keypad (Fig96). 【ESC/STOP】to finish inputting and exit. Fig 96 3.
  • Page 45 Fig 97 4. Press 【F3】 to set tolerance (Fig 98). Input the value by pressing the numeric keypad. Fig 98 5. Press 【ZERO】for blanking. 6. Put the sample (calibrated holmium liquid) into Main Light Path. Press 【START】 to check. The results will be displayed on the screen (Fig 99).
  • Page 46 Connect to PC Press【8】in Fig 73 to connect to PC (Fig 100). If the instrument is controlled by PC, the screen displays as Fig 100A. Press【ESC/STOP】to exit. Fig 100 Fig 100A Beeper on/off Press【9】in Fig 73 to turn on/off the beeper Delete entire saved files Press 【...

  • Page 47: Instrument Maintenance

    Instrument Maintenance To keep the instrument work in good condition, constant maintenance is needed. Daily Maintenance 1, Check the compartment After measurement, the cuvettes with sample solutions should be taken out of the compartment in time. Or the volatilization of the solution would make the mirror go moldy.

  • Page 48: Spare Parts Replacement

    Spare parts replacement 1, Replace the Fuse Danger! Be sure to switch off the power and unplug the socket before replacement! Step 1: Tools preparation Prepare a 3×75mm Flat Blade Screwdriver Step 2：Switch Off the power supply Switch off the power supply, and unplug the socket. Step 3: Take out the Fuse Seat Take out the Fuse Seat by the Screwdriver.
  • Page 49 Step 4: Remove the cover of the Lamp Chamber Unscrew the screws of the Lamp Chamber indicated in Fig.103 and remove its cover. (Fig 103) Fig 103 W Lamp Step 5: Replace Lamps D2 Lamp Connector Motor D2 Lamp Fig 104 Top View of Lamp Chamber 1) Replace D2 Lamp Unscrew the 2 screws on the D2 Flange (Indicated in the Red Circles in Fig 105), unplug the power connector（Indicated in the Red Square in...
  • Page 50 Fig. 105 2) Replace W lamp Remember the direction of the filament before pull out the W lamp. Be sure that the new lamp’s filament is in the same direction as before. Pull out the defected W lamp and draw on the Cotton Glove. Insert the new W lamp as deep as possible on the Lamp Seat.
  • Page 51 Fig.107 Step 8 Finish Reset the cover of the Lamps chamber and fix the screws. Reset the cover of the instrument and fix the screws. then the course finished.
  • Page 52 Appendix A DNA/Protein Test Algorithm Test Name Method Calculations Displayed Wavelength(s) Parameters Units DNA MEASUREMENT =62.9 DNA: Absorbance 260nm difference concentration: =36.0 DNA/Protein μg/ml 280nm (260,280) =1552 Protein:μ 320nm Concentration Protein =757.3 (optional) g/ml concentration DNA purity Absorbance =49.1 260nm difference concentration: =3.48...
  • Page 53 Appendix B A number of correction techniques can be used to eliminate or reduce interference errors. In general, if the source of the error is known and is consistent from sample to sample, the error can be eliminated. On the other hand, if the source is unknown and varies from sample to sample, the error can be reduced but not eliminated.
  • Page 54 A.2 Three-point correction The three-point, or Morton-Stubbs correction uses two reference wavelengths, usually those on either side of the analytical wavelength. The background interfering absorbance at the analytical wavelength is then estimated using linear interpolation (see Figure A2).This method represents an improvement over the single-wavelength reference technique because it corrects for any background absorbance that exhibits a linear relationship to the wavelength.
  • Page 55 Drawell International Technology Limited Shanghai Drawell Scientific Instrument Co.,Ltd Add : Suite 1506,Lane581 XiuChuan Rd.,PuDong New Area,Shanghai,China Tel: 0086 21 54411195 Fax: 0086 21 33823261 Web : www.drawell.com.cn Email : sales01@drawell.com.cn...

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