Guidelines For Good Pipetting - Gilson MyPIPETMAN Select Manual

Guidelines for Good Pipetting

●
Make sure that you operate the push button slowly and smoothly.
●
When aspirating, keep the tip at a constant depth below the surface of the liquid
(refer to the table below).
Model
P2, P10, P8x10, P12x10
P20, P8x20, P12x20, P8x200, P12x200
P8x300, P12x300
P1000
P5000
P10mL
● Change the tip before aspirating a different liquid, sample, or reagent.
●
Change the tip if a droplet remains at the end of the tip from the previous pipetting operation.
●
Pre-wet each new tip with the liquid to be pipetted.
●
The liquid should never enter the tip holder; to prevent this:
○ Press and release the push button slowly and smoothly.
○ Never turn the pipette upside down.
○
Never lay the pipette on its side when there is liquid in the tip.
●
If you use the same tip with a larger volume, pre-wet the tip.
●
For volatile solvents, saturate the air cushion in your pipette by aspirating and dispensing the
solvent repeatedly before aspirating the sample.
●
When the liquid temperature is different from the ambient temperature, pre-wet the tip several
times before use.
●
You may remove the tip ejector (refer to
tubes.
●
After pipetting acids or other corrosive liquids that emit vapors, remove the tip ejector and tip
holder, rinse with distilled water, dry, and lubricate the piston (refer to MAINTENANCE). For the
P1000L model, use a specific tip holder equipped with a filter to increase the lifetime of the piston
(refer to ACCESSORIES).
●
Do not pipette liquids with temperatures above 70°C or below 4°C. The pipette can be used
between +4°C and +40°C, but the gravimetric performance may vary according to the temperature
(refer to the ISO 8655-2 standard for conditions of use).
MyPIPETMAN should be held in the vertical position. For detailed information about pipetting techniques,
NOTICE
refer to the
10
|
PIPETTING MyPIPETMAN®
Immersion Depth
(Millimeters)
1
2-3
2-4
2-4
3-6
5-7
Fit the Tip Ejector
Gilson Guide to Pipetting available on the Gilson website (www.gilson.com).
Wait Time
(Seconds)
1
1
1
2-3
4-5
4-5
Adapter) to aspirate from very narrow