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- turn the "EVAC-AIR" switch to "AIR", wait for about 10 seconds (Two sounds)
- remove the sample holder (avoid touching the sample holder beyond the O-ring)
Load your sample
Do not touch to the left of the O-ring for this is the vacuum side of the holder.
Fix the holder with the holder fixing table.
Flip the end of the sample holder up and insert your grid with the darker side up.
Flip the end of the sample holder down to secure the sample.
Check to make sure the sample will not fall out of the holder
Insert the sample holder
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