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Related Manuals for cytiva Oligo Column
Summary of Contents for cytiva Oligo Column
- Page 1 Adjustable Oligo Column Operating Instructions cytiva.com...
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Page 2: Table Of Contents
Reference information ..................31 Specification ..............................32 Chemical resistance ............................. 33 Recycling information ..........................35 Regulatory information ..........................36 Ordering information ........................... 37 Health and Safety Declaration Form ...................... 38 Index ........................... 40 Adjustable Oligo Column Operating Instructions 28967444 AC... - Page 3 Page intentionally left blank...
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Page 4: Introduction
This chapter contains important user information, intended use of Adjustable Oligo Column, and lists of abbreviations used in this documentation. In this chapter Section See page Important user information About this manual Abbreviations Adjustable Oligo Column Operating Instructions 28967444 AC... -
Page 5: Important User Information
Primer Support ™. The column is designed for maximum scalability and coupling efficiency with a flexible column volume. Adjustable Oligo Column is intended for use with ÄKTA™ OligoPilot™ plus 100. Adjustable Oligo Column must not be used in any clinical procedures, or for diagnostic purposes. Prerequisites In order to operate the product in the way it is intended: •... -
Page 6: About This Manual
The Operating Instruction manual provides information needed to install, operate and maintain the product in a safe way. Scope of this manual This manual describes how to use Adjustable Oligo Column. The illustration below shows Adjustable Oligo Column. Adjustable Oligo Column Operating Instructions 28967444 AC... -
Page 7: Abbreviations
This section explains abbreviations that appear in the user documentation for the product. Abbreviations Abbreviation Meaning Column volume EPDM Ethylene propylene diene monomer ETFE Ethylene-tetrafluoroethylene Internal diameter Outer diameter PEEK Polyetheretherketone PTFE Polytetrafluoroethylene Tetrahydrofurane Adjustable Oligo Column Operating Instructions 28967444 AC... -
Page 8: Safety Instructions
Safety precautions Symbols and abbreviations Important WARNING Before installing, operating or maintaining the product, all users must read and understand the entire contents of this chapter to become aware of the hazards involved. Adjustable Oligo Column Operating Instructions 28967444 AC... -
Page 9: Safety Precautions
2.1 Safety precautions Safety precautions Introduction Adjustable Oligo Column is used with materials that can be hazardous. Before installing, operating or maintaining the product, you must be aware of the hazards described in this manual. Follow the instructions to avoid injury to the operator or other personnel, damage to samples or other substances handled by the equipment, to the product, or to other equipment in the area. - Page 10 WARNING Use only approved parts. Only spare parts and accessories that are approved or supplied by Cytiva may be used for maintaining or servicing the product. WARNING Do not use the product if it is not working properly, or if it has suffered any damage, for example if damage is caused by dropping the equipment.
- Page 11 Operation of the product outside these limits can damage equipment and cause personal injury or death. CAUTION No other solvents than those specified in this manual should be used as they could possibly damage the column. Adjustable Oligo Column Operating Instructions 28967444 AC...
- Page 12 WARNING Hazardous chemicals during maintenance. When using hazardous chemicals for system or column cleaning, wash the system or columns with a neutral solution in the last phase or step. Adjustable Oligo Column Operating Instructions 28967444 AC...
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Page 13: Symbols And Abbreviations
Warning! Read the user documentation before using the column. Do not open any covers or replace parts unless specifically stated in the user documentation. Mfg. Year Year (YYYY) and month (MM) of manufacture Adjustable Oligo Column Operating Instructions 28967444 AC... -
Page 14: Product Description
This chapter gives an overview of Adjustable Oligo Column. Column design The Adjustable Oligo Column has a simple design. It comprises a number of complete units which are easily assembled together. The column tube is manufactured from borosilicate glass. It is held in place between the top end-piece and bottom end-piece by three support rods. - Page 15 3 Product description Column illustration Part Function Securing ring adapter Connection point to the column Prefilter unit including O-ring Tubing D-flange O-ring, 64.5×3 mm diameter Adjustable Oligo Column Operating Instructions 28967444 AC...
- Page 16 3 Product description Part Function O-ring, 55×1.6 mm diameter Glass column tube Distribution unit Securing ring Connection point from the column Tie rod and hex-nut Adjustable Oligo Column Operating Instructions 28967444 AC...
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Page 17: Unpacking And Installation
On delivery of the column, perform the following steps. Step Action Open the box and carefully remove the column. Inspect the column for any damage. Report any damage to your local Cytiva representative immediately. Adjustable Oligo Column Operating Instructions 28967444 AC... -
Page 18: Operation
Do not overtighten PTFE tubing as this will damage the end of the tubing. WARNING Never use pressure limits above the column specification. CAUTION Do not use solvents other than those specified in this manual, as this could result in damage to the column. Adjustable Oligo Column Operating Instructions 28967444 AC... - Page 19 5 Operation NOTICE To avoid precipitation keep all reagents at or above 18°C. Adjustable Oligo Column Operating Instructions 28967444 AC...
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Page 20: Preparation
Flow rate conversions The following table shows the unit conversion of flow velocity in cm/hour into flow rate mL/min for Adjustable Oligo Column. cm/hour mL/min 28.26 70.65 141.3 188.4 Adjustable Oligo Column Operating Instructions 28967444 AC... -
Page 21: Pack The Column
The Primer Support must be packed as a slurry. The exact ratio is 0.111 gram Primer Support 5G per mL column volume. Calculation to determine the CV: 3 × 3 × 3.14 × bed height in cm = CV in mL Adjustable Oligo Column Operating Instructions 28967444 AC... - Page 22 Start the UNICORN™ method instruction PFlow AB (10 bar flow) on the connected system. Continue for 1.5 CV to 2.0 CV. Stop the flow. Start the run. Adjustable Oligo Column Operating Instructions 28967444 AC...
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Page 23: Unpack And Empty The Column
5 Operation 5.3 Unpack and empty the column Unpack and empty the column Unpacking procedure To unpack the Primer Support and empty Adjustable Oligo Column follow these steps: Step Action Disconnect the column from the system. Place a suitably dimensioned collecting vessel under the column to collect the acetonitrile. -
Page 24: Maintenance
6 Maintenance Maintenance About this chapter This chapter describes how to clean and maintain the column in a safe way. The Adjustable Oligo Column is designed with a minimum of components to facilitate cleaning and operation. In this chapter Section... -
Page 25: Disassemble And Assemble For Cleaning And Maintenance
This section describes how to disassemble the column for cleaning and maintenance, and how to assemble it afterwards. Disassembly To disassemble Adjustable Oligo Column unscrew the adapter counterclockwise by hand and pull it out of the column body. Assembly To assemble Adjustable Oligo Column follow these steps:... -
Page 26: Cleaning
2 × 100 mL/min is started, the pressure reading on the pump display or UNICORN should be 3 ± 0.5 bar g, depending on the length of the tubing connected to the adapter. Adjustable Oligo Column Operating Instructions 28967444 AC... -
Page 27: Part Replacement
Turn the head of the column clockwise until the adapter rests on the packed bed. Continue to turn the column head until the bed is compressed as much as possible. Adjustable Oligo Column Operating Instructions 28967444 AC... - Page 28 6 Maintenance 6.3 Part replacement O-ring replacement Two different types of O-rings are used in the Adjustable Oligo Column. Use a spatula to pry out worn O-rings. Adjustable Oligo Column Operating Instructions 28967444 AC...
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Page 29: Troubleshooting
This troubleshooting guide is intended to aid the operator if problems arise. If the problem cannot be solved after consulting this guide, contact your local Cytiva representative for advice. Potential problems This table lists solutions to the most common problems with Adjustable Oligo Column. Problem Possible cause Solution... -
Page 30: Reference Information
8 Reference information Reference information About this chapter This chapter lists the technical specifications of Adjustable Oligo Column. The chapter also includes a chemical resistance guide, recycling information, regulatory information, ordering information, and Health and Safety Declaration forms for service. -
Page 31: Specification
8 Reference information 8.1 Specification Specification Introduction This section describes characteristics of Adjustable Oligo Column. General specification Part Specification Net mesh size 20 μm Flow rate range 0 to 200 mL/min Design pressure 20 bar g Recommended 1 to 8 cm bed heights The design pressure is the sum of packing and operating pressures. -
Page 32: Chemical Resistance
Concentrations are expressed as % volume of saturated solution unless otherwise stated. Adjustable Oligo Column can be expected to be resistant to the chemicals listed below. Chemical... - Page 33 8 Reference information 8.2 Chemical resistance Chemical Concentration Sodium hydroxide Trifluoroacetic 0.1% acid NOTICE Do not use THF, tetrahydrofurane, in Adjustable Oligo Column. Adjustable Oligo Column Operating Instructions 28967444 AC...
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Page 34: Recycling Information
Recycling of hazardous substances The product contains hazardous substances. Detailed information is available from your Cytiva representative. Disposal Make sure to recycle the different materials of the column according to local regulations. Adjustable Oligo Column Operating Instructions 28967444 AC... -
Page 35: Regulatory Information
The table below summarizes the required manufacturing information. Requirement Information Name and address of manufacturer Cytiva Sweden AB Björkgatan 30 SE 751 84 Uppsala Sweden Telephone number of manufacturer + 46 771 400 600 Adjustable Oligo Column Operating Instructions 28967444 AC... -
Page 36: Ordering Information
8 Reference information 8.5 Ordering information Ordering information Product codes For information about product codes and parts related to Adjustable Oligo Column visit cytiva.com. This section describes additional parts and product numbers for Adjustable Oligo Column. Product Pack size Product code... -
Page 37: Health And Safety Declaration Form
Service Ticket #: To make the mutual protection and safety of Cytiva service personnel and our customers, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your equipment, complete this checklist and present it to the Service Engineer upon arrival. - Page 38 To make sure the mutual protection and safety of Cytiva personnel, our customers, transportation personnel and our environment, all equipment must be clean and free of any hazardous contaminants before shipping to Cytiva. To avoid delays in the processing of your equipment, complete this checklist and include it with your return.
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Page 39: Index
System description, 15 Intended use, 6 System label, 14 description, 14 Labels, 14 System label, 14 Troubleshooting, 30 Manufacturing information, 36 Unpack column, 24 Unpacking, 18 Notes and tips, 6 Weight, 32 O-ring replacement, 29 Adjustable Oligo Column Operating Instructions 28967444 AC... - Page 40 Standard Software End-User License Agreement is available on request. All goods and services are sold subject to the terms and conditions of sale of the supplying company operating within the Cytiva business. A copy of those terms and conditions is available on request. Contact your local Cytiva representative for the most current information.
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