YSI 1500 Sport User Manual
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YSI 1500 Sport User Manual

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User's Manual
YSI 1500 Sport
L L L L - - - - Lactate Analyzer
Lactate Analyzer
Lactate Analyzer
Lactate Analyzer
www.YSI.com

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  • Page 1 User's Manual YSI 1500 Sport L L L L - - - - Lactate Analyzer Lactate Analyzer Lactate Analyzer Lactate Analyzer www.YSI.com...

  • Page 3: Table Of Contents

    Preparing the Fluid System..................3-18 Printer Option ......................3-19 Sample Collection, Preparation and Measurement......4-1 Sample Preparation ...................... 4-1 YSI Model 1501, 25 microliter Syringepet ..............4-2 YSI Model 1502 Capillary Tube Injector ................ 4-3 Principles of Operation ................ 5-1 Sensor Technology ...................... 5-1 Fluid System ........................
  • Page 4 Microprocessor Self Test ....................5-9 Electrode Checks ......................5-9 Temperature Error Checks ..................... 5-10 Battery Level Checks ..................... 5-10 1500 SPORT Menu Flow Chart..................5-11 Instrument Operation ................6-1 Calibration Cycle ......................6-1 Calibration-Automatic Mode ................... 6-1 Calibration-Manual Mode....................6-5 Sample Cycle .......................
  • Page 5 Fluid System Faults ......................9-9 Possible Failure Conditions ..................9-10 Appendix A–Typical Performance Data ..........10-1 Capillary Tube Injector, YSI Model 1502 ..............10-1 Blunt Needle Syringepet, YSI Model 1501 ..............10-3 Appendix B–Sample Collection and Handling ........11-1 Blood Plasma ......................... 11-1 Blood Serum ........................
  • Page 6 Reducing Agents......................14-1 Calibration Shift by Detergents ..................14-2 Endogenous and Exogenous Substances ............... 14-3 Physical Damage......................14-3 Appendix F–Temperature Effect on Operation ........15-1 Temperature Effect On Battery Life ................15-1 Appendix G–Accessory Products ............16-1 Appendix H–Cleaning, Disinfecting and Decontamination ....17-1 Appendix I–Warranty and Shipping Information ......

  • Page 7: Safety

    1. Safety 1.1 Explanation of Symbols Warning indicates that misuse of the instrument could result WARNING in death or serious injury to a person. Caution, consult accompanying documents. Caution indicates that misuse of the instrument could result in mild CAUTION or serious injury to a person and/or damage to equipment.
  • Page 8 1 1 1 1 - - - - 2 2 2 2...

  • Page 9: Introduction

    2. Introduction The YSI 1500 SPORT is a portable, battery powered blood lactate analyzer intended for professional use in sports medicine applications. The SPORT is not intended for home use. It is designed to provide quick measurement of lactate in whole blood or plasma.

  • Page 10: Specifications

    Specifications Sample size: 25 microliters Sample Medium: Whole Blood; Plasma Sampling Speed: Manual Mode: 60 seconds Auto Mode: 96 seconds Detection range: 0 to 30 mmol/L (0 to 270 mg/dL) Resolution: 0.01 mmol/L (0.2 mg/dL) Calibration point: 5 mmol/L (45 mg/dL) Typical performance: See Appendix A for typical performance data for whole blood and plasma.

  • Page 11: Instrument Setup

    Reagents and injection devices for the SPORT analyzer are not packaged in the same carton as the instrument. Reagents come in a starter kit (YSI 1504); and injection devices (YSI 1501 or 1502) must be ordered separately.

  • Page 12: Component Identification

    3.2 Component Identification This section names the principal parts and describes their use. Figure 3.1 and Figure 3.2 identify each part. Figure Figure 3 3 3 3 ..1 1 1 1 SPORT SPORT Figure Figure SPORT SPORT 3 3 3 3 - - - - 2 2 2 2...
  • Page 13 Injection Port is on the top of the Sample Chamber. Inside the port is a sensor that detects the insertion of a YSI injection device. A removable cap is threaded into the top of the Injection Port. This cap must be removed and replaced with the supplied threaded plug before moving or storing the instrument.
  • Page 14 Section 8.2 Preventative Maintenance. If your sample measuring protocol requires the total lysis of blood cells, or if you are going to preserve the samples in YSI 2372 Preservative Tubes for later analysis, you must refer to Appendix D Sample Preservation, for further information before proceeding.

  • Page 15: Reagent Preparation

    Prepare 500 mL of Buffer solution, pour it into the Reference Bottle and add to it 4.5 cc of YSI 1530 30 mmol/L Calibration Standard. This should be measured out with the graduated syringe supplied in the 1504 Starter Kit. Replace the bottle lid and shake the bottle for 15 seconds to insure complete mixing.

  • Page 16: Membrane Installation

    (YSI 2392 is provided in the 1504 starter kit). Place one drop of salt solution on the probe face. Then, using the plastic membrane pack as an applicator, press the O-ring membrane assembly gently onto the probe face (Figure 3.4).

  • Page 17: System Setup

    If the SPORT is stored with the power on and the charger disconnected, the battery will discharge to a level requiring a recharge within 3 days. YSI recommends that the SPORT be used with the battery charger whenever AC power is available. IMPORTANT: IMPORTANT: Probe polarization will be disrupted when power is turned off.
  • Page 18 Menu Cancel Numeric Keypad Delete Enter Figure Figure 3 3 3 3 ..5 5 5 5 SPORT Keypad SPORT Keypad Figure Figure SPORT Keypad SPORT Keypad How the five function keys and the numeric keypad are used: •...

  • Page 19: Date

    Date Date Date Date Go to Main menu by pressing M M M M ENU ENU until you see: RUN- - - - 0 RECALL 0 RECALL- - - - 1 1 1 1 0 RECALL 0 RECALL SETUP SETUP SETUP SETUP- - - - 2 DIAG 2 DIAG...

  • Page 20: Time

    Time Time Time Time The SPORT incorporates a 24 hour clock. Go to Main menu by pressing MENU MENU until you see: MENU MENU RUN- - - - 0 RECALL 0 RECALL- - - - 1 1 1 1 0 RECALL 0 RECALL SETUP SETUP- - - - 2 DIAG...

  • Page 21: Rs232 Communication

    RS232 Communication RS232 Communication RS232 Communication RS232 Communication It is possible to send all of the stored sample data to a personal computer, but in order to do so a communication software package is required to be installed in the PC. Once the communication package is installed in the PC, it is simply a matter of making sure the settings of the PC and the SPORT are the same.

  • Page 22: Setting The Lcd Contrast

    Select stop bits by pressing 2 2 2 2 , then ENTER ENTER. ENTER ENTER STOP BITS STOP BITS STOP BITS STOP BITS 1 1 1 1 - - - - 0 2 0 2- - - - 1 1 1 1 Select the desired setting by number, then ENTER ENTER ENTER...

  • Page 23: Displaying Numerical Values In Radix

    and then the ENTER ENTER key. The adjustment will increment with each pressing of ENTER ENTER the ENTER ENTER key. ENTER ENTER Press MENU MENU until the Main menu reappears. MENU MENU Displaying Numerical Values in RADIX Displaying Numerical Values in RADIX Displaying Numerical Values in RADIX Displaying Numerical Values in RADIX The default setting shows a period separating the integer part of the number...

  • Page 24: Selecting The Displayed Units

    RUN- - - - 0 RECALL 0 RECALL- - - - 1 1 1 1 0 RECALL 0 RECALL SETUP SETUP- - - - 2 DIAG 2 DIAG- - - - 3 3 3 3 SETUP SETUP 2 DIAG 2 DIAG Select Setup by pressing 2 2 2 2 , then ENTER ENTER ENTER.

  • Page 25: Selecting The Operational Mode

    Select More by pressing 3 3 3 3 , then ENTER ENTER. ENTER ENTER UNITS UNITS- - - - 0 MODE 0 MODE- - - - 1 1 1 1 UNITS UNITS 0 MODE 0 MODE SET DEFAULT SET DEFAULT- - - - 2 2 2 2 SET DEFAULT SET DEFAULT Select Units by pressing ENTER...

  • Page 26: Resetting System Default Parameters

    SET CAL MODE AUTO SET CAL MODE AUTO- - - - 0 0 0 0 SET CAL MODE AUTO SET CAL MODE AUTO MANUAL MANUAL- - - - 1 1 1 1 MANUAL MANUAL Select Auto mode by pressing ENTER ENTER ENTER ENTER, or select Manual mode by pressing 1 1 1 1 ,...
  • Page 27 DATE DATE- - - - 0 TIME 0 TIME- - - - 1 1 1 1 DATE DATE 0 TIME 0 TIME RS232 RS232- - - - 2 MORE 2 MORE- - - - 3 3 3 3 RS232 RS232 2 MORE 2 MORE Select More by pressing 3 3 3 3 , then ENTER...

  • Page 28: Preparing The Fluid System

    3.7 Preparing the Fluid System Once you have setup the electronics of the instrument, filled both supply bottles, and emptied the waste bottle, you are ready to prime the fluid system. You must return to the Main menu. RUN- - - - 0 RECALL 0 RECALL 0 RECALL- - - - 1 1 1 1 0 RECALL...

  • Page 29: Printer Option

    3.8 Printer Option While there is not a printer built into the instrument, there is a optional printer available either through YSI (Model 1506), or your local Hewlett Packard dealer. This printer, HP Model 82240, is a battery-powered unit controlled by an infrared signal that is generated from the SPORT.
  • Page 30 A similar printout is generated when calibrating and checking linearity. We recommend that the printer be used in this manner whenever possible for two reasons. One, if there were a complete electrical failure or accidental re- formatting of the memory, it could be possible that the sample results in memory may be lost or irretrievable.

  • Page 31: Sample Collection, Preparation And Measurement

    The blood from a finger or earlobe prick should be collected in a capillary tube. If you use one of the YSI tubes, which are preset to 25 microliters, you must inject it with the capillary tube injector. You can also draw venapuncture blood from a vial, vacutainer, or YSI Preservative Tube with the preset tube.

  • Page 32: Ysi Model 1501, 25 Microliter Syringepet

    YSI Model 1501, 25 microliter Syringepet YSI Model 1501, 25 microliter Syringepet YSI Model 1501, 25 microliter Syringepet YSI Model 1501, 25 microliter Syringepet The Syringepet can be used to extract a 25 microliter sample from either a vacutainer or a capillary tube of greater volume than 25 microliters, see Figure 4.1.

  • Page 33: Ysi Model 1502 Capillary Tube Injector

    YSI Model 1502 Capillary Tube Injector YSI Model 1502 Capillary Tube Injector YSI 1505 capillary tubes are provided in the Starter Kit. These tubes, which can be ordered separately, are preset to a volume of 25 microliters. There is a double white band on the tube opposite the end that you put into the sample.
  • Page 34 When you adjust the clamp, it is not necessary to unscrew it completely. Never should more than one full turn be necessary. WARNING: WARNING: WARNING: WARNING: Any sample carryover on the outside of the tube could affect the measurement. You should wipe off the tube or Syringepet needle with a sterile wipe, like a Kimwipe.

  • Page 35: Principles Of Operation

    Ohio. The immobilized enzyme membrane was invented by YSI and is covered by U.S. Patent 4,073,713. This sensor technology has been used successfully since 1975 in the YSI 23A Blood Glucose Analyzer; then later in the YSI 27 Industrial Analyzer, the YSI 23L Blood Lactate Analyzer, the YSI 2300 STAT Glucose/L- Lactate Analyzer, and the YSI 2700 SELECT Chemistry Analyzer.
  • Page 36 Reaction 1 (lactate): Lactate + O Pyruvate + H Reaction 2: H + 2e Pt anode The platinum electrode is held at an anodic potential and is capable of oxidizing many substances other than H . To prevent these reducing agents from contributing to sensor current, the membrane contains an inner layer consisting of a very thin film of cellulose acetate.

  • Page 37: Fluid System

    5.2 Fluid System Figure Figure Figure Figure 5 5 5 5 ..2 2 2 2 SPORT Fluid System SPORT Fluid System SPORT Fluid System SPORT Fluid System 5 5 5 5 - - - - 3 3 3 3...

  • Page 38: Measurement Methodology

    5.3 Measurement Methodology The algorithm used to calculate results allows the SPORT to process samples as quickly as possible while maintaining high accuracy and reliability. The result is calculated from a group of four readings, depending on the chosen operational mode: •...
  • Page 39 Figure Figure Figure Figure 5 5 5 5 ..3 3 3 3 Automatic Mode Probe Current Profile Automatic Mode Probe Current Profile Automatic Mode Probe Current Profile Automatic Mode Probe Current Profile 5 5 5 5 - - - - 5 5 5 5...

  • Page 40: Baseline Stability

    Figure Figure Figure Figure 5 5 5 5 ..4 4 4 4 Manual Mode Probe Current Profile Manual Mode Probe Current Profile Manual Mode Probe Current Profile Manual Mode Probe Current Profile 5.4 Baseline Stability The SPORT checks the probe baseline current for stability whenever it enters the Sample mode.

  • Page 41: Calibration

    5.5 Calibration The SPORT uses one lactate standard (YSI 2327 5 mmol/L) for calibration and two lactate standards are available for checking linearity (YSI 2328 15mmol/L and YSI 1530 30 mmol/L). The following is the recommended calibration frequency: Auto Mode...

  • Page 42: Linearity

    Aging Membrane Response You can check linearity with YSI 1530 30 mmol/L standard, after every calibration. You can also check it at "mid-range" with YSI 2328 15 mmol/L standard. Both standards should be injected as if it were a sample.

  • Page 43: Temperature Compensation

    If this does not correct the problem, try turning the main power switch off and on again. If this does not help, call YSI Technical Support for repair assistance. Also at power up and prior to each sample cycle, the processor does a fast self test of the electronics to see if any stored data has been corrupted, or if the software has been physically changed.

  • Page 44: Temperature Error Checks

    Temperature Error Checks Temperature Error Checks Temperature Error Checks Temperature Error Checks The sample chamber temperature is checked at several different times during sampling to assure that the rate of change is not adversely affecting result reliability. If the error limits are exceeded, an appropriate error message will be displayed.

  • Page 45: 1500 Sport Menu Flow Chart

    1500 SPORT Menu Flow Chart 1500 SPORT Menu Flow Chart 1500 SPORT Menu Flow Chart 1500 SPORT Menu Flow Chart Main Menu RUN-0 RECALL-1 SETUP-2 DIAG-3 Run Mode Recall Mode Setup Mode Diagnostic Mode Sample Display Date Probe Current Time...
  • Page 46 5 5 5 5 - - - - 1 1 1 1 2 2 2 2...

  • Page 47: Instrument Operation

    As a result of this YSI recommends that in the first hour of use after As a result of this YSI recommends that in the first hour of use after...
  • Page 48 Press MENU MENU MENU until the following display appears. MENU RUN- - - - 0 RECALL 0 RECALL 0 RECALL- - - - 1 1 1 1 0 RECALL SETUP SETUP SETUP SETUP- - - - 2 DIAG 2 DIAG 2 DIAG 2 DIAG- - - - 3 3 3 3 Select Run by pressing ENTER...
  • Page 49 1 PRIME 1 PRIME Check the linearity of the membrane by injecting a sample of either the YSI 2328 15 mmol or the YSI 1530 30 mmol lactate standard. 6 6 6 6 - - - - 3 3 3 3...
  • Page 50 Press ENTER ENTER ENTER, the display will read... ENTER ENTER ID# XXX ENTER ID# XXX ENTER ID# XXX ENTER ID# XXX No ID# is required so press ENTER ENTER, the display will read... ENTER ENTER WAIT... WAIT... WAIT... WAIT... INJECT SAMPLE... INJECT SAMPLE...

  • Page 51: Calibration-Manual Mode

    If the result is not within these ranges, replace the membrane, allow time for it to break in and repeat the calibration process. If the result is acceptable press MENU MENU MENU MENU and the display will read SAMPLE SAMPLE SAMPLE SAMPLE- - - - 0 0 0 0 CAL- - - - 1 PRIME...
  • Page 52 If the baseline probe current is not within the expected range the display will read: ERROR ERROR: BASELINE : BASELINE ERROR ERROR : BASELINE : BASELINE PRESS MENU PRESS MENU PRESS MENU PRESS MENU If you see this display press MENU MENU MENU MENU, then see Section 9 Troubleshooting.
  • Page 53 1 PRIME- - - - 2 2 2 2 1 PRIME 1 PRIME Check the linearity of the membrane by injecting a sample of either the YSI 2328 15 mmol or the YSI 1530 30 mmol lactate standard. Press ENTER...

  • Page 54: Sample Cycle

    MM/DD/YY HH:M MM/DD/YY HH:M MM/DD/YY HH:M MM/DD/YY HH:MM M M M #XXX XX.XXmmol/L #XXX XX.XXmmol/L #XXX XX.XXmmol/L #XXX XX.XXmmol/L The sample result will be displayed along with the ID#, date and time. Linearity failure means that the instrument is not performing to specifications at the upper limit of the dynamic range.
  • Page 55 Select the Sample cycle by pressing ENTER ENTER ENTER ENTER. The memory is limited to 150 results. You can continue to run samples while the memory is full, but the results will not be saved. Stored results will have to be deleted in order to make room available.

  • Page 56: Recalling Stored Results

    If the concentration of the sample exceeds the measurable limits of the instrument, the following message will appear: ERROR: OVERRANGE ERROR: OVERRANGE ERROR: OVERRANGE ERROR: OVERRANGE WASHING... WASHING... WASHING... WASHING... If you see this display, no result will be stored or displayed, press MENU and refer to Section 9 Troubleshooting.

  • Page 57: Recalling Today's Sample Results

    Select Recall by pressing 1 1 1 1 , then ENTER ENTER. ENTER ENTER SAMPLES LCD SAMPLES LCD- - - - 0 0 0 0 SAMPLES LCD SAMPLES LCD PRINT PRINT- - - - 1 XMIT 1 XMIT- - - - 2 2 2 2 PRINT PRINT 1 XMIT...

  • Page 58: Recalling A Result From Any Previous Date

    SAMPLES LCD SAMPLES LCD SAMPLES LCD SAMPLES LCD- - - - 0 0 0 0 PRINT PRINT PRINT PRINT- - - - 1 XMIT 1 XMIT 1 XMIT 1 XMIT- - - - 2 2 2 2 To select viewing the results on the display press ENTER ENTER.
  • Page 59 SAMPLES LCD SAMPLES LCD SAMPLES LCD SAMPLES LCD- - - - 0 0 0 0 PRINT PRINT PRINT PRINT- - - - 1 XMIT 1 XMIT 1 XMIT- - - - 2 2 2 2 1 XMIT To select viewing the results on the display press ENTER ENTER.

  • Page 60: Recalling A Result By Id Number

    Recalling a Result By ID Number Recalling a Result By ID Number Recalling a Result By ID Number Recalling a Result By ID Number Go to Main menu by pressing the MENU MENU key until the display reads. MENU MENU RUN- - - - 0 RECALL 0 RECALL 0 RECALL- - - - 1 1 1 1...

  • Page 61: Printing Results

    NOTE: NOTE: NOTE: NOTE: It is possible to end the result recall at any point by pressing MENU MENU MENU MENU. When you are finished, press MENU MENU until the Main menu appears. MENU MENU 6.4 Printing Results It is possible to print out results as you are running samples. This is possible because the SPORT sends all of the result data over the infrared data link automatically at the end of each sample cycle.

  • Page 62: Printing Today's Results

    NOTE: NOTE: The printer must be placed in the correct position and turned on in order NOTE: NOTE: to receive the data. Refer to Figure 3.6 for the printer placement if you are unsure. Printing Today's Re Printing Today's Results Printing Today's Re Printing Today's Re sults...

  • Page 63: Printing Previous Days' Results

    Printing Previous Days' Results Printing Previous Days' Results Printing Previous Days' Results Printing Previous Days' Results Open the Main menu. RUN- - - - 0 RECALL 0 RECALL 0 RECALL 0 RECALL- - - - 1 1 1 1 SETUP SETUP- - - - 2 DIAG 2 DIAG- - - - 3 3 3 3 SETUP...

  • Page 64: Transmitting Results Over The Rs232 Communication Link

    Select Recall by pressing 1 1 1 1 , then ENTER ENTER. ENTER ENTER SAMPLES LCD SAMPLES LCD- - - - 0 0 0 0 SAMPLES LCD SAMPLES LCD PRINT PRINT- - - - 1 XMIT 1 XMIT- - - - 2 2 2 2 PRINT PRINT 1 XMIT...

  • Page 65: Deleting Stored Results

    The display will be active during the transmitting process, flashing each result as it is transmitted. All stored data will be transmitted and it is not possible to it is not possible to it is not possible to it is not possible to halt the process once it has started.

  • Page 66: Deleting All Results

    The results that need to be deleted can be displayed by date, by ID#, or all the results may be displayed. To delete the displayed result, press the DELETE DELETE DELETE key DELETE and the result will be deleted from the memory. The display will then step to the next result.

  • Page 67: Daily Operational Checks

    Linearity Test Linearity Test Linearity Test Use YSI 1530 30 mmol/L or YSI 2328 15 mmol/L Lactate Standard to check the linearity of the sensor. These standards are packaged in your starter kit, and also may be ordered separately. Acceptable Ranges:...
  • Page 68 6 6 6 6 - - - - 22 22 22 22...

  • Page 69: Instrument Diagnostics

    7. Instrument Diagnostics Diagnostic routines in the software provide troubleshooting capability not only in manufacturing, but also in field service and user maintenance. These routines check the following elements of the electronic and fluid systems. • Probe current. Buffer and reference fluid cycles are independently accessible to monitor probe current change.

  • Page 70: Checking Sample Chamber Temperature

    WAIT... WAIT... WAIT... WAIT... PROBE X.XX nA PROBE X.XX nA PROBE X.XX nA PROBE X.XX nA The display will update every few seconds. To leave this condition, press MENU. The display will read: MENU. MENU. MENU. PROBE PROBE- - - - 0 0 0 0 PROBE PROBE REF- - - - 1 BUF...

  • Page 71: Checking The Sample Chamber Injection Switch Status

    Select Temperature by pressing 1 1 1 1 , then ENTER ENTER. The display will show the ENTER ENTER current chamber temperature. CHAMBER TEMP XX.XX CHAMBER TEMP XX.XX CHAMBER TEMP XX.XX CHAMBER TEMP XX.XX C C C C Press MENU MENU to return to the Main menu.

  • Page 72: Turning Pump Motors On And Off

    Select More by pressing 3 3 3 3 , then ENTER ENTER. ENTER ENTER STIR STIR- - - - 0 PUMPS 0 PUMPS- - - - 1 1 1 1 STIR STIR 0 PUMPS 0 PUMPS PRINT PRINT- - - - 2 MORE 2 MORE- - - - 3 3 3 3 PRINT PRINT...

  • Page 73: Sending A Test Print String Over The Infrared Data Link

    7.6 Sending a Test Print String Over the Infrared Data Link Go to Main menu by pressing MENU MENU MENU until you see: MENU RUN- - - - 0 RECALL 0 RECALL 0 RECALL 0 RECALL- - - - 1 1 1 1 SETUP SETUP SETUP...

  • Page 74: Testing The Keypad

    Select Diagnostics by pressing 3 3 3 3 , then ENTER ENTER. ENTER ENTER PROBE PROBE- - - - 0 TEMP 0 TEMP- - - - 1 1 1 1 PROBE PROBE 0 TEMP 0 TEMP INJ SW INJ SW- - - - 2 MORE 2 MORE- - - - 3 3 3 3 INJ SW INJ SW...

  • Page 75: Testing The Display

    STIR STIR- - - - 0 PUMPS 0 PUMPS- - - - 1 1 1 1 STIR STIR 0 PUMPS 0 PUMPS PRINT PRINT- - - - 2 MORE 2 MORE- - - - 3 3 3 3 PRINT PRINT 2 MORE 2 MORE Select More by pressing 3 3 3 3 , then ENTER...

  • Page 76: Checking The Software Version Number

    7.10 Checking the Software Version Number It will be necessary to communicate the software version number to a service technician if a call is required. Press MENU MENU MENU MENU until you return to the Main menu. RUN- - - - 0 RECALL 0 RECALL- - - - 1 1 1 1 0 RECALL 0 RECALL...
  • Page 77 Select Diagnostics by pressing 3 3 3 3 , then ENTER ENTER. ENTER ENTER PROBE PROBE- - - - 0 TEMP 0 TEMP- - - - 1 1 1 1 PROBE PROBE 0 TEMP 0 TEMP INJ SW INJ SW- - - - 2 MORE 2 MORE- - - - 3 3 3 3 INJ SW INJ SW...
  • Page 78 7 7 7 7 - - - - 10 10 10 10...

  • Page 79: Maintenance

    8. Maintenance Turn the instrument power off during maintenance. This will prevent any accidental activation of the fluid pumps which could cause spills or air in the tubing. 8.1 Daily Maintenance Certain procedures are to be followed each day that the SPORT is in use. Empty the Waste Bottles Empty the Waste Bottles Empty the Waste Bottles...

  • Page 80: Check For Leaks

    IMPORTANT: IMPORTANT: bottle and the 3cc Syringe provided in the YSI 1504 Starter Kit should be cleaned every week with a disinfecting agent. The bottles and Syringe should be rinsed completely with distilled water after cleaning to remove any disinfecting residue, which would cause membrane failure if it got into the system.

  • Page 81: Membrane Replacement

    Reassemble the chamber and manifold and attach the assembly to the case panel. Reattach the injection block to the top of the sample chamber. Remember to return the stir bar to the chamber. Install new O-rings or a new membrane if necessary, and install the probes. Extra O-rings are supplied in your Preventative Maintenance Kit.

  • Page 82: Tubing Replacement

    3. Prepare a small amount of 0.5% sodium hypochlorite solution. 4. Immerse the sensor in the solution for 30 - 45 seconds. Check periodically...sensor should darken, but do not allow it to become black. 5. Remove the sensor and immediately rinse it with reagent water for at least 2 minutes.
  • Page 83 Waste Supply Waste Supply Figure Figure 8 8 8 8 - - - - 1 1 1 1 SPORT Tubing Routing Figure Figure SPORT Tubing Routing SPORT Tubing Routing SPORT Tubing Routing WARNING: It is extremely important that the correct size tubing be used for WARNING: WARNING: WARNING:...

  • Page 84: Injection Port Seal Replacement

    8.7 Injection Port Seal Replacement An injection port seal is included with your instrument. It is used during transportation and long term storage. Replace the plug and O-ring on it every six months, because they can deteriorate and allow chamber fluid leakage. Extra O- rings and plugs are supplied in the Preventative Maintenance Kit.
  • Page 85 YSI recommends that prior to storing, the battery should be fully charged (8 hours minimum) and the power turned off.

  • Page 87: Troubleshooting

    FAX for assistance, please include a thorough description of the problem(s) and copies of the printed reports if possible. If it becomes necessary to return the instrument to YSI for servicing please refer to Appendix I–Warranty and Shipping Information for instructions.

  • Page 88: Fault Messages

    CONSULT MANUAL CONSULT MANUAL We recommend at this point that you contact YSI Customer Service as this is a system failure that may require returning the instrument. The second option is to select YES which will reformat the SPORT memory.
  • Page 89 We recommend at this point that you contact YSI Customer Service as this is a system failure that may require returning the instrument. The second option is to select YES which will reformat the SPORT memory. The software will attempt to correct itself but in doing so ALL stored data will be lost.

  • Page 90: Battery Faults

    Battery Faults Battery Faults Battery Faults Battery Faults LOW BATTERY LOW BATTERY LOW BATTERY LOW BATTERY PRESS ON PRESS ON PRESS ON PRESS ON A check of the battery voltage is done during the sample cycle. At 12.1 volts this message is displayed.
  • Page 91 • POSSIBLE CAUSE: A sample has been injected into the sample chamber at the improper time. • CORRECTIVE ACTION: Enter PROBE diagnostics and cycle the buffer pump. • SECTION: 7.1 • POSSIBLE CAUSE: Pinched, plugged, leaking or disconnected tubing. • CORRECTIVE ACTION: Fix or replace tubing. •...
  • Page 92 • POSSIBLE CAUSE: Newly installed enzyme membrane. • CORRECTIVE ACTION: Enter PROBE diagnostics and check probe current. • SECTION: 7.1 • POSSIBLE CAUSE: Newly installed enzyme probe. • CORRECTIVE ACTION: Enter PROBE diagnostics and check probe current. • SECTION: 7.1 •...
  • Page 93 • POSSIBLE CAUSE: Failing enzyme membrane. • CORRECTIVE ACTION: Perform daily operational checks and replace if necessary. • SECTION: 6.7 • POSSIBLE CAUSE: Pinched, plugged, leaking or disconnected tubing. • CORRECTIVE ACTION: Fix or replace tubing. • SECTION: 8.6 • POSSIBLE CAUSE: Calibrator solution out of spec: contaminated or in use for more than 30 days.

  • Page 94: Temperature Faults

    This fault is generated when the sample concentration exceeds the upper limit of the dynamic range (30 mmol). No result will be stored in memory. • POSSIBLE CAUSE: Sample concentration is too high, resulting in high probe current. • CORRECTIVE ACTION: Dilute sample 1:1 with the same buffer solution being used in the instrument and repeat.

  • Page 95: Fluid System Faults

    CONCENTRATION LO This warning is generated when the Reference solution concentration is calculated to be less than 80% of the injected Calibration standard (YSI 2327 5 mmol/L Lactate Std.). It is possible that significantly high or low Reference solution concentrations can have a negative effect on the performance of the SPORT at the upper most part of the dynamic range.

  • Page 96: Possible Failure Conditions

    If it occurs during calibration calibration it is because the measured reference current after calibration calibration the calibrator injection is different from the measured reference current after the linearity injection by more than 5%. If it occurs during sampling sampling sampling it is because the measured reference current after sampling the sample injection is different from the measured reference current after the...
  • Page 97 ** The stir bar is driven by a spinning magnetic coupling, if the speed is increased the coupling will be interrupted and the stir bar will tumble and vibrate fairly violently. If bubble will still not release, take a small diameter pin or other device (paper clip works well) and stick it into the sample chamber through the injection port and interrupt the stir bar motion.
  • Page 98 Sample chamber overflows through the injection port. Sample chamber overflows through the injection port. Sample chamber overflows through the injection port. Sample chamber overflows through the injection port. • POSSIBLE CAUSE: Waste tubing line in pumps crimped or stuck shut. •...
  • Page 99 • POSSIBLE CAUSE: Stir motor failure. • CORRECTIVE ACTION: Go to stir motor diagnostics and verify. • SECTION: 7.4 9 9 9 9 - - - - 13 13 13 13...
  • Page 100 9 9 9 9 - - - - 14 14 14 14...

  • Page 101: Appendix A-Typical Performance Data

    10. Appendix A–Typical Performance Data Testing of the YSI Model 1500 SPORT vs. the YSI Model 2300 STAT suggests the following typical performance: Capillary Tube Injector, YSI Model 1502 Capillary Tube Injector, YSI Model 1502 Capillary Tube Injector, YSI Model 1502...
  • Page 102 16.0 14.0 12.0 10.0 10.0 14.0 18.0 22.0 26.0 30.0 YSI 2300 ( mmol/l ) Y = 1.0095X - .0224 DATA Whole Blood Lactate - Extracellular ONLY Concentration Concentration Concentration Concentration Pooled Est. Std. Dev. Pooled Est. Std. Dev. Pooled Est. Std. Dev.

  • Page 103: Blunt Needle Syringepet, Ysi Model 1501

    18.0 22.0 26.0 30.0 YSI 2300 ( mmol/l ) Y = 1.0078X - .0714 DATA Total Blood Lactate - YSI 1515 cell lysing agent added to the buffer solution in both instruments. Concentration Concentration Concentration Concentration Pooled Est. Std. Dev.
  • Page 104 16.0 14.0 12.0 10.0 10.0 14.0 18.0 22.0 26.0 30.0 YSI 2300 ( mmol/l ) Y = 1.0076X - .1302 DATA Whole Blood Lactate - Extracellular ONLY Concentration Concentration Concentration Concentration Pooled Est. Std. Dev. Pooled Est. Std. Dev. Pooled Est. Std. Dev.
  • Page 105 18.0 22.0 26.0 30.0 YSI 2300 ( mmol/l ) DATA Y = 0.9793X - .1345 Plasma Lactate - YSI 1515 cell lysing agent added to the buffer solution in both instruments. Concentration Concentration Concentration Concentration Pooled Est. Std. Dev. Pooled Est. Std. Dev.
  • Page 106 22.0 26.0 30.0 YSI 2300 ( mmol/l ) Y = 0.9893X - .1559 DATA Plasma Lactate - Without YSI 1515 cell lysing agent added to the buffer solution of either instrument. Concentration Concen tration Pooled Est. Std. Dev. Pooled Est. Std. Dev.

  • Page 107: Appendix B-Sample Collection And Handling

    11. Appendix B–Sample Collection and Handling The YSI 1500 SPORT is not intended for home use. Proper sample collection and preparation, by a professional, are vital factors in obtaining reliable results with the YSI SPORT. This section covers the basic guidelines for collecting and preparing whole blood, plasma and serum samples.

  • Page 108: Blood Serum

    Sodium fluoride and oxalate in combination in a total concentration of 2 mg/ml of blood. Heparin sodium as commonly supplied in Vacutainer collection tubes. WARNING: The YSI SPORT is not suitable for use with samples containing thymol or other phenol preservatives. These are interfering substances.

  • Page 109: Whole Blood

    WARNING: The YSI Model 1500 is not suitable for use with samples containing thymol or other phenol preservatives. These are interfering substances. Warning: Frozen blood-bank blood, which contains a high percentage of glycerine, is not a suitable specimen for diagnostic work with the Model 1500.
  • Page 110 WARNING: The YSI SPORT is not suitable for use with samples containing thymol or other phenol preservatives. These are interfering substances. Before taking a sample from a whole blood specimen, any cells which may have settled out must be resuspended. Invert the collection tube three times, gently enough not to cause frothing, just before sampling.

  • Page 111: Appendix C-Cell Lysing

    12. Appendix C–Cell Lysing Obtaining reliable results that have value requires a basic understanding of what is going on inside the whole blood sample, at least as it relates to lactate. Lactate is always present in the blood plasma at some level due to minor anaerobic activity.

  • Page 112: Using The Ysi 1515 Cell Lysing Agent

    If your sample measuring protocol requires the total lysis of blood cells, or if you are going to preserve the samples with the YSI 2315 Preservative Tube Kit for later analysis, another step is required in the reagent preparation process explained in Section 3.3 Reagent Preparation.
  • Page 113 WARNING: If you switch from running with, to running without the lysing agent, or vice versa, you cannot get reliable results unless you allow sufficient time for complete removal of the agent from the fluid system. 12 12 12 12- - - - 3 3 3 3...
  • Page 114 12 12 12 12- - - - 4 4 4 4...

  • Page 115: Appendix D Sample Preservation

    SPORT when running samples preserved in YSI preservative tubes. You cannot use the YSI preservative tubes unless the lysing agent is used in the instrument when the actual measurement takes place. This is due to the fact that the preservative tubes contain a detergent. This detergent, when introduced into a system without the cell lysing agent, can affect the membrane response and cause incorrect readings.
  • Page 116 13 13 13 13- - - - 2 2 2 2...

  • Page 117: Appendix E-Effects Of Selected Substances

    Many reducing agents would give rise to a false signal current (and falsely elevated reading) if they succeeded in reaching the sensing anode of the YSI probe. Most of these are excluded from the probe by the cellulose acetate layers...

  • Page 118: Calibration Shift By Detergents

    The following reducing agents may result in an elevated background current when present at low levels (only a few milligrams per liter): Aniline Phenol Thiocarboyhdrazide Thiourea Resorcinol Guaiacol 2-Mercaptoethanol Ethyl Carbazate Formic Acid Hydrazide Catechol Acetaminophen The following materials may cause trouble at higher concentrations: 2-Amino-4-nitrophenol Hydroquinone p-Cresol...

  • Page 119: Physical Damage

    YSI has tested hundreds of substances to determine whether they have any effect on the sensor system used in the SPORT. YSI has also verified that the addition of Triton X-100 to the SPORT instrument buffer does not significantly alter the extent to which these materials interfere with the instrument.
  • Page 120 Substance Weight Lactate Interfering Level mg/dL mmol/L Lactic Acid Analogues DL-a-Glycerophosphate,Disodium 216.4 -- -- DL-Glyceric Acid, hemi-calcium 286.25 1276 Glycolic Acid 76.05 B-Hydroxypyruvic Acid,Lithium salt -- -- DL-B-Hydroxybutyric Acid,Sodium salt -- -- 4-Hydroxybutyric Acid,Sodium salt 126.09 -- -- DL-Malic Acid 134.09 -- -- DL-a-Hydroxybutyric Acid...
  • Page 121 Substance Weight Lactate Interfering Level mg/dL mmol/L Drugs, Poisons, and Miscellaneous Exogenous Substances: Acetaminophen 151.17 Metaphosphoric Acid -- -- Ethanol 46.07 -- -- Methanol 32.04 -- -- Iso-Propanol 60.10 -- -- Ethylene Glycol 62.07 -- -- Acetylsalicylic Acid 180.16 -- -- Formaldehyde 30.03 2606...
  • Page 122 14 14 14 14- - - - 6 6 6 6...

  • Page 123: Appendix F-Temperature Effect On Operation

    It may be possible that in some situations the 1500 SPORT may appear to be overly sensitive to "TEMPERATURE STABILITY ERRORS". If that is the case, there are simple precautions that should be taken to insure the quality of the results.
  • Page 124 15 15 15 15- - - - 2 2 2 2...

  • Page 125: Appendix G-Accessory Products

    16. Appendix G–Accessory Products The following products are available from YSI or your sales representative. YSI Catalog YSI Catalog YSI Catalog YSI Catalog Number Number Description Description Number Number Description Description 1501 25 microliter, Blunt Needle Syringepet 1502 25 microliter, Capillary Tube Injection Device...
  • Page 126 16 16 16 16- - - - 2 2 2 2...

  • Page 127: Appendix H-Cleaning, Disinfecting And Decontamination

    17. Appendix H–Cleaning, Disinfecting and Decontamination Proper precautionary lab practices should be followed when handling biological hazards. Suggested cleaning and disinfecting solutions: Suggested cleaning and disinfecting solutions: Suggested cleaning and disinfecting solutions: Suggested cleaning and disinfecting solutions: • Isopropanol 70% •...
  • Page 128 17 17 17 17- - - - 2 2 2 2...

  • Page 129: Appendix I-Warranty And Shipping Information

    Centers ce Centers United States YSI Incorporated • Repair Center • 1725 Brannum Lane • Yellow Springs, OH • 45387 • USA Phone: 937 767-7241 • Fax: 937 767-9353 Europe Analytical Technologies • Lynchford House • Lynchford Lane • Farnborough, Hampshire • GU14 GLT • England Phone: 441 252 514711 •...

  • Page 130: Cleaning Instructions

    We will contact the sender for instructions as to the disposition of the equipment. Disposition costs will be the responsibility of the sender. When service is required, either at the user's facility or at YSI, the following steps must be taken to insure the safety of our service personnel.

  • Page 131: Packing Instructions

    Packing Instructions Packing Instructions Packing Instructions Packing Instructions 1. Clean and decontaminate items to insure the safety of the handler. 2. Complete and include the Cleaning Certificate. 3. Place the product in a plastic bag to keep out dirt and packing material. 4.
  • Page 132 18 18 18 18- - - - 4 4 4 4...

  • Page 133: Appendix J-Required Notice

    19. Appendix J–Required Notice The Federal Communications Commission defines this product as a computing device and requires the following notice: This equipment generates and uses radio frequency energy and if not installed and used properly, may cause interference to radio and television reception. It has been type tested and found to comply with the limits for a Class A or Class B computing device in accordance with the specification in Subpart J of Part 15 of FCC Rules, which are designed to provide reasonable protection against such...
  • Page 134 YSI Incorporated 1725 Brannum Lane Yellow Springs, Ohio 45387 USA 937 767-7241 • 800 659-8895 Fax 937 767-8058 Info@ysi.com www.YSI.com CEPartner4U BV Esdoornlaan 13 3951DB Maarn The Netherlands +31 (0) 6-516.536.26 015162 A15162H 2003-12  2003 YSI Incorporated...

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