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Olympus BH-2 Manual

Phase contrast
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Revision
1
Phase Contrast on the Olympus BH-2 Microscopes
Phase Contrast on the
Olympus BH-2 Microscopes
Copyright © 2021 Carl Hunsinger
Description of Changes
Initial release
Revision 1
The content of this document is provided for
Revision History
Revision 1
Date
May 5, 2021
Page 1 of 6

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Summary of Contents for Olympus BH-2

  • Page 1 Revision History Revision Description of Changes Date Initial release May 5, 2021 Phase Contrast on the Olympus BH-2 Microscopes Revision 1 Page 1 of 6...

  • Page 2: Table Of Contents

    Figure 7 – The phase ring and phase annulus images ......................5 Figure 8 – The phase annulus properly centered ........................5 Figure 9 – Place green/yellow filter into filter receptacle ...................... 6 Phase Contrast on the Olympus BH-2 Microscopes Revision 1 Page 2 of 6...

  • Page 3: Introduction

    Phase contrast microscopy utilizes special optics This document describes the proper procedures to set (both in the condenser and in the objectives) to up an Olympus BH-2 microscope for phase contrast accomplish two things: 1) The S wave-front (i.e., the viewing.

  • Page 4: Match Selector Dial With The Low-Power Objective

    Use the “CT-5” for 23mm tubes and use the “CT-30” for 30mm tubes. Note that other manufacturer’s centering telescopes may be substituted for the Olympus CT, so long as the barrel diameter of the phase telescope is compatible with your ocular tubes.

  • Page 5: Center The Selected Phase Annulus

    45mm filter receptacle beneath condenser (see Figure 6) until the thumbscrews engage the stage (see Figure with the internal centering screws for the selected Phase Contrast on the Olympus BH-2 Microscopes Revision 1 Page 5 of 6...

  • Page 6: Using Phase Contrast

    Please feel free to direct any questions or comments that the filter imparts may be removed from the images regarding this document (or BH-2 microscopes in by converting them to grayscale. general) to the author as listed on the cover page of this For routine viewing, however, you may find that you document.