Optical Filters - Edmund Optics TECHSPEC Step-By-Step Instructions

OPTICAL FILTERS

A basic fluorescence microscope requires 3
filters. Each application will use a different
combination depending on the system
requirements:
• Excitation Filter: placed within the
illumination path of a fluorescence
microscope. It filters out all wavelengths of the
light source except for the excitation range of
the fluorophore or specimen under inspection.
• Dichroic Filter: placed between the excitation
filter and emission filter at a 45° angle. It
reflects the excitation signal towards the
fluorophore under inspection and transmits the
emission signal towards the detector.
• Emission Filter: placed within the imaging
path of a fluorescence microscope. It filters out
the entire excitation range of the fluorophore
under inspection and transmits the emission
range of the fluorophore.
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