Download
Share
Print this page
  1. Manuals
  2. Brands
  3. Edmund Optics Manuals
  4. Microscope
  5. TECHSPEC
  6. Step-by-step instructions

Edmund Optics TECHSPEC Step-By-Step Instructions

A fluorescence microscope
Hide thumbs

Advertisement

Quick Links

Download this manual
STEP-BY-STEP INSTRUCTIONS FOR
BUILDING A FLUORESCENCE
MICROSCOPE
TECHSPEC®
Optical Cage System

Advertisement

loading
Need help?

Need help?

Do you have a question about the TECHSPEC and is the answer not in the manual?

Questions and answers

Summary of Contents for Edmund Optics TECHSPEC

  • Page 1 STEP-BY-STEP INSTRUCTIONS FOR BUILDING A FLUORESCENCE MICROSCOPE TECHSPEC® Optical Cage System...
  • Page 2 Since infinity-corrected digital video microscopes can be quite complex, using the modular TECHSPEC Optical Cage System is ideal for simplifying the assembly. In this case another aspect has been added to the design to incorporate a fluorescence aspect to the digital microscope.
  • Page 3 OVERVIEW Complete Parts List Filter Selection Sub Assemblies: 1. Dichroic Assembly 2. Objective Arm 3. Source Arm 4. Imaging Arm Mounting & Alignment PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.

  • Page 4: Complete Parts List

    25mm Dia. 20mm Efl. Aspheric Lens EO-3112C ½” CMOS Color USB Camera 59367 59382 5m USB2.0 (mini-B to standard-A) Cable 85618 Micrometer Mount Kit 58661 Micrometer Head Anvil 13mm PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.

  • Page 5: Optical Filters

    • Emission Filter: placed within the imaging path of a fluorescence microscope. It filters out the entire excitation range of the fluorophore under inspection and transmits the emission range of the fluorophore. PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 6 1. DICHROIC ASSEMBLY PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 7 Cage Sphere with (5) 30mm Port and (1) 43mm Port 85632 25mm Standard Cage Plate with M6 for Post Mounting 85737 30mm Square Interface Plate 86018 Filter Holder Plate PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 8 Keep the the openings of the plates facing the 43mm port. Attach a Square Mounting Plate (#85-737) to a side opposite of a filter holder plate. PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 9 2. OBJECTIVE ARM PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 10 30mm Cage Plate C-mount Adapter 85490 Cage Support Rod 6mm Diameter X 150mm 85488 Cage Support Rod 6mm Diameter x 100mm Length 85-618 Micrometer Mount Kit 58-661 Micrometer Head Anvil 13mm PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 11 6. Keep the ends flush with the plate and secure with M3 set screws. 8. Slide the 2 springs from the micrometer assembly onto the 100mm long rods. PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 12 M3 set screws keeping the springs under tension. The loose square plate should now translate when the micrometer is adjusted. This loose square plate will serve as the microscope stage. PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 13 3. SOURCE ARM PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 14 Cage Support Rod 6mm Diameter x 100mm Length, M3 85630 30mm Standard Cage Plate with M6 for Post Mounting 65553 25mm Dia. 25mm Efl. Achromatic Lens 66014 25mm Dia. 20mm Efl. Aspheric Lens PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 15 Note: there is an extra plate shown in these images between the achromat and filter holder. This is 17. Temporarily secure the plates on the rods for later optional for mounting purposes. alignment. PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 16 4. IMAGING ARM PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 17 54633 C-Mount Extension Tube (100mm Length) 54632 C-Mount Extension Tube (50mm Length) 03625 C-Mount Fine Thread Extension Tube (30mm-50mm Length) EO-3112C ½” CMOS Color USB Camera 59367 PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 18 15. Thread the C-Mount Fine Thread Extension Tube, 100mm C-mount Extension Tube, and 50mm C-Mount Extension Tube (#03-625, #54-632, #54- 633) onto the male threading of the tube lens assembly. PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 19 486) to connect the Square Plate attached to tube lens to the Filter Holder Plate opposite the objective on the sphere. Secure rods with M3 set screws. PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 20 MOUNTING & ALIGNMENT PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 21 Attach mounting posts to the two new plates and adjust the placement of the plates to match the breadboard or bench plate spacing. PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 22 • Once the microscope is assembled and aligned, the excitation and emission filters can be secured within two Interchangeable Filter Holders (#85-649). The Filter Holders slide into the filter holder plates for inspection. PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.
  • Page 23 START BUILDING NOW! www.edmundoptics.com/cage-system • Instructional Videos • Application Examples • 3D Models • Demos • and Much More PROPRIETARY - Property of Edmund Optics, Inc. | 2012 Copyright© Edmund Optics, Inc.