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Summary of Contents for Zeiss Cell Observer spinning disk confocal
- Page 1 Wellcome Trust Centre for Human Genetics Cellular-Imaging Microscopy Core The Zeiss Spinning Disk Confocal Abridged INSTRUCTIONS Page | 1...
- Page 2 Introduction The Zeiss Cell Observer spinning disk confocal microscope is located in the category three viral suite in the Oxford Particle Imaging Centre (STRUBI). The spinning disk’s principle advantage over our Leica SP8 and Zeiss 510 LSM microscopes is speed of acquisition rather than image quality, and so the spinning disk is particularly suited to live cell imaging, although it can image specimens on slides as well.
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Page 3: General Information
65,536 grey levels (with 0 being pure black- and 65,535 being pure white). The Evolve camera’s fastest frame rate is 15ms (67 frames per second). Images are stored in the Zeiss *.czi format. To view these *.czi files on another Windows 7 PC download Zen Lite via the Core’s Spinning Disk microscope webpages. - Page 4 Using the Zeiss Observer Z1 microscope The Cell Observer microscope mixes air (10x), oil (100x), and water objectives (40x and 63x). Make sure you use the appropriate immersion fluid with the objective. The immersion oil for the 100x oil is left on the air table and the Immersol W 2010 for water immersion is left under the PC monitor.
- Page 5 Using the Zeiss Observer XL stage incubator The Zeiss 37oC incubator controller must be switched on before any other equipment otherwise the control link to the microscope stand won’t be established and ‘Incubation’ will not appear as a tab in the Observer Z1’s LCD controller.
- Page 6 • Press the button "valve" again to deactivate the CO2 control. The LED in the button is off. The PeCon CO2 controller is on loan from the Zeiss 510 confocal system and may have be used on other microscope systems occasionally (principally with the Leica SP8 confocal).
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Page 7: Switch On Procedure
HXP-120V short-arc mercury lamp power module Switch on all the other 13A mains socket switches. All the microscope control boxes should come on (always leave the Zeiss modules in the switched on position). The Yokogawa spinning head will also come on (the key is left in the on position). -
Page 8: Switch Off Procedure
Switch off procedure Remove the specimen from the microscope stage Wipe off excess immersion fluid from the objective lens/top using the Whatman 105 lens cleaning tissues provided The 10x air objective doesn’t need cleaning after use Leave the 10x objective selected on the microscope nose-piece to minimise any possibility of damage during start-up when the XY motorised stage initialises Drop the 10x objective to the Load (lowest) position using the... - Page 9 If you have any microscope/imaging problems or queries, just ask a member of the Microscopy Core staff for help. There is additional help and advice on our Core web-pages: http://www.well.ox.ac.uk/microscopy WTCHG Zeiss Cell Observer SD microscope user guide. This version updated 28 November 2014 . Page | 9...
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