Zeiss Cell Observer spinning disk confocal Abridged Instructions page 2

Introduction
The Zeiss Cell Observer spinning disk confocal microscope is located in the
category three viral suite in the Oxford Particle Imaging Centre (STRUBI). The
spinning disk's principle advantage over our Leica SP8 and Zeiss 510 LSM
microscopes is speed of acquisition rather than image quality, and so the
spinning disk is particularly suited to live cell imaging, although it can image
specimens on slides as well.
The new Zeiss Cell Observer spinning disk confocal microscope has:
Zeiss Observer Z1 Inverted Microscope
•
NewPort Vision IsoStation anti-vibration isolation table
•
Yokogawa CSU-X1 Filter Wheel and Spinning Disk Unit
•
Photometrics Evolve 512 Delta EM-CCD camera (512 x 512 pixels)
•
Applied Scientific MS2000 Piezo motorised XY stage
•
Applied Scientific fast galvo Z focus (150um range)
•
Definite Focus with NIR LED Hardware Focus to eliminate thermal drift
•
PeCon Temp Module S1 and XL Multi S1 live cell stage incubator
•
o
(rated 3 C to 45
10x EC Plan Neofluar NA 0.3 air objective
•
40x C-Apochromat NA 1.2 water-immersion objective (with coverslip
•
correction collar)
63x C-Apochromat NA 1.2 UV-VIS-IR water-immersion objective (with
•
coverslip correction collar)
100x Plan Apochromat NA 1.4 oil-immersion objective
•
Zeiss HXP-120V short-arc fluorescence Hg lamp and controller (2,000h)
•
DAPI, GFP and DSRed diochroic filters for eyepiece viewing
•
405nm line DAPI laser
•
480nm line GFP and 468nm line DsRed laser
•
Zeiss ZEN Blue image aquisition software
•
o
C)
Page | 2