Endress+Hauser Analytik Jena InnuPure C16 touch Operating Manual
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Endress+Hauser Analytik Jena InnuPure C16 touch Operating Manual

Endress+Hauser Analytik Jena InnuPure C16 touch Operating Manual

Extraction system
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InnuPure C16 touch
Extraction System

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Summary of Contents for Endress+Hauser Analytik Jena InnuPure C16 touch

  • Page 1 Operating manual InnuPure C16 touch Extraction System...
  • Page 2 Analytik Jena AG Manufacturer Konrad-Zuse-Str.1 07745 Jena  Germany Phone + 49 3641 77 70 + 49 3641 77 9279 Email info@analytik-jena.de Analytik Jena AG Technical Service Konrad-Zuse-Str. 1 07745 Jena  Germany Phone + 49 3641 77 7407 + 49 3641 77 7449 Email info@analytik-jena.de Analytik Jena AG...

  • Page 3: Table Of Contents

    InnuPure C16 touch Contents Contents Basic information ....................... 5 User manual notes ......................5 Intended use ........................6 Warranty and liability ....................... 6 Safety instructions ...................... 7 General notes ........................7 Safety markings and notice signs on the InnuPure C16 touch ........7 Technical condition ......................
  • Page 4 Contents InnuPure C16 touch 6.7.4 Updating IPextract ......................50 6.7.5 Managing user-defined sample types ................51 6.7.6 Selecting the time zone / adapting daylight ..............52 Managing data ........................ 52 Maintenance and care ....................55 Cleaning the InnuPure C16 touch .................. 55 Exchanging fuses ......................

  • Page 5: Basic Information

    InnuPure C16 touch Basic information Basic information User manual notes The InnuPure C16 touch is intended for operation by qualified specialist personnel ob- Contents serving this user manual. The user manual informs about the design and operation of the InnuPure C16 touch and provides the personnel familiar with the isolation and purification of nucleic acid with the necessary expertise for the safe handling of the equipment and its compo- nents.

  • Page 6: Intended Use

    Basic information InnuPure C16 touch Intended use The InnuPure C16 touch is a flexible and efficient extraction system for the fully auto- mated isolation and purification of nucleic acid in chemical and biological laboratories. The device is intended for general laboratory use. The InnuPure C16 touch including its original accessories must only be used for the ap- plications described in this instruction manual.

  • Page 7: Safety Instructions

    InnuPure C16 touch Safety instructions Safety instructions General notes For your own safety and to ensure error-free operation of the InnuPure C16 touch, please read this chapter carefully before using the appliance. Observe all safety notes listed in this user manual and all messages and notes dis- played by the IPextract control program on the screen.

  • Page 8: Technical Condition

    Safety instructions InnuPure C16 touch Number Warning / notice symbol Meaning Biohazard Observe the operating manual Reaching inside the device prohibited during op- eration Danger due to crushing by moving parts Warning against electric voltage Before opening disconnect the mains plug Before opening the device always disconnect the mains plug China only:...

  • Page 9: Safety Instructions, Transport And Installation

    InnuPure C16 touch Safety instructions The instruction also includes imparting the contents of this user manual. Furthermore, the user manual must be accessible to the operating and service personnel at any time! Safety instructions, transport and installation Only transport the device in its original packaging! Ensure that all transport protec- tions have been fitted and the device is completely empty, cleaned and decontami- nated.

  • Page 10: Safety Instructions Relating To Ambient Conditions

    Safety instructions InnuPure C16 touch 2.6.2 Safety instructions relating to ambient conditions  Working with explosive substances in this device or operating the device in an ex- plosive environment is prohibited.  Smoking or open flames in the operating room of the InnuPure C16 touch are pro- hibited! Observe the flammable ingredients (ethanol-containing buffers) of the kits.

  • Page 11: Chemical Resistance Of The Device

    InnuPure C16 touch Safety instructions 2.6.5 Chemical resistance of the device Aggressive substances may damage the device. Although the materials used are re- sistant to most of the commonly used substances, material damage from aggressive substances cannot be precluded.  Before using any aggressive substances (e.g. acids, bases, organic solutions), check whether the materials in direct contact with the substances are resistant to them.

  • Page 12: Function And Setup

    Function and setup InnuPure C16 touch Function and setup Principle of operation The InnuPure C16 touch is a flexible and efficient extraction system for the fully auto- mated isolation and purification of nucleic acid. The system, developed and produced in Germany, has been designed for low sample throughput. A large selection of differ- ent source materials can be used.

  • Page 13: Isolation And Purification Of Nucleic Acids With Magnetic Beads Kits

    InnuPure C16 touch Function and setup Isolation and purification of nucleic acids with magnetic beads kits Pre-filled reagent structure Reagent structure The reagent strips or reagent plates are completely pre-filled and contain, in addition to the magnetic particles, all reagents necessary for the extraction process for the binding, washing and elution of nu- cleic acids.
  • Page 14 Function and setup InnuPure C16 touch Transferring the washing buffer Washing of the nucleic acids The magnetic particles remain in the work cavity. The washing buffers are transferred there. By pipet- ting different washing buffers on and off the nucleic acids are washed.

  • Page 15: Isolation And Purification Of Nucleic Acids With Smartextraction Kits

    InnuPure C16 touch Function and setup Transferring elution buffer in the work cavity Elution The elution volume can be adjusted in the range of 20 – 500 µl, depending on the extraction protocol, and is defined at the start of the protocol. The correspondingly selected volume is aspirated from the holding position and transferred to the magnetic particles in the work cavity.
  • Page 16 Function and setup InnuPure C16 touch Pre-filled reagent structure Reagent structure The reagent strips or reagent plates of the SmartExtraction kits are completely pre-filled and contain all reagents necessary for the extraction process for the binding, washing and elution of nucleic acids.
  • Page 17 InnuPure C16 touch Function and setup Ethanol removal The removal of ethanol residues from the samples in the pipette tip is realized via a drying step. In this step, a heating is activated at the cavity floor, causing ethanol residues to evaporate. As an alternative the ethanol is dissolved in the rins- ing liquid by a short mixing step and is so removed.

  • Page 18: Design Of The Innupure C16 Touch

    Function and setup InnuPure C16 touch Design of the InnuPure C16 touch The InnuPure C16 touch is a stand-alone device. The device is equipped with a com- bined magnet/heating unit. This allows for the nucleic acids that bind to magnetic and paramagnetic particles to be effectively collected at the cavity floor while the washing solution, for example, evaporates at temperatures of up to 70 °C.
  • Page 19 InnuPure C16 touch Function and setup Fig. 3 Device view with open front door Sample tray counter-holder Protection cover of the combined magnet/heating element Drawer Cones tip attachment During operation, the front door of the InnuPure C16 touch is closed. The device is equipped with an LED for status control.
  • Page 20 Function and setup InnuPure C16 touch Fig. 5 Priming station The loaded sample tray is placed on the drawer and moved by the software to the cor- rect position inside the InnuPure C16 touch. The prefilled and sealed kits must be pierced manually.

  • Page 21: Installation And Commissioning

    InnuPure C16 touch Installation and commissioning Installation and commissioning Installation conditions For requirements with regard to the ambient conditions at the installation location, re- Ambient conditions fer to “Technical data” p. 66. If required, make sure that the room is temperature- controlled.

  • Page 22: Connections And Control Elements

    Installation and commissioning InnuPure C16 touch For insertion of the sample tray, the lower front door opens automatically and soft- ware-controlled. The drawer is ejected at the front of the device. The device door must also be opened for cleaning and maintenance purposes and must therefore be added to the dimensions.
  • Page 23 InnuPure C16 touch Installation and commissioning  Safety symbols  Year of manufacture  Device number The 10” tablet with touchscreen is located at the front of the device. It is used to con- trol the InnuPure C16 touch. The tablet is attached via a support bracket to the basic device and can be easily mounted and dismounted.

  • Page 24: Connecting The Innupure C16 Touch And Switching It On/Off

    Installation and commissioning InnuPure C16 touch Connecting the InnuPure C16 touch and switching it on/off ATTENTION Retain the transport packaging! Return transport for service must be in the original packaging. This alone prevents transport damage. 1. Remove the InnuPure C16 touch from the packaging. Connecting the device 2.
  • Page 25 InnuPure C16 touch Installation and commissioning The status LED is located on the front of the device above the front door (Fig. 2 p. 18). Status LED LED signal Device Status Flashing green light Ongoing initialization Steady green light The device is operational Flashing green light Ongoing application (tool or extraction) Steady red light...

  • Page 26: Sample Preparation

    Sample preparation InnuPure C16 touch Sample preparation Preparing buffers and solutions All buffers and solutions are provided in pre-filled and sealed reaction tubes (reagent plates or strips) depending on the extraction routine and source material. The prepa- ration of the sample tray is described in detail in each kit manual and in the sections “Preparing the sample tray for kits with magnetic beads”...
  • Page 27 InnuPure C16 touch Sample preparation 2. Insert the reagent plate into the sample block of the sample tray. The chamfered corners must point to the red dot on the holder. 3. Place the adapter for the reagent strips into the sample block.
  • Page 28 Sample preparation InnuPure C16 touch 6. Place the filter tips into the two rows with the smaller drill holes. Place the elution tubes into the wider drill holes at the edge of the tip block. Empty sample positions shall not be filled. With the strips, make sure that for every strip the tips and the elution tube are in the corresponding positions in the tip block (arrows in figure on the left)!

  • Page 29: Preparing The Sample Tray For Smartextraction

    InnuPure C16 touch Sample preparation Preparing the sample tray for SmartExtraction CAUTION Nickel allergy! The sample tray is coated with nickel. ATTENTION Use the priming station to populate the sample tray. The filter tips are longer than the tip block and would impact against the tabletop! Prior to extraction, the sample tray must be loaded with the reagent plates and/or rea- gent strips of the kit used.
  • Page 30 Sample preparation InnuPure C16 touch 3. Place the adapter for the reagent strips into the sample block. The red marking on the adapter must point to the red point on the sample block. Attention: Load both blocks of the sample tray evenly (see below)! 4.
  • Page 31 InnuPure C16 touch Sample preparation 7. Check the SmartExtraction filter tips. Make sure that the granulate for adsorption of the bacterial DNA is distributed evenly and without gaps and sits close to the tip outlet. Tap the tip with your finger or shake the tip to dislodge the granulate.
  • Page 32 Sample preparation InnuPure C16 touch For an extraction run, it is necessary to always populate both sample blocks of the Notes on sample tray sample tray to ensure that the tray is balanced and to allow for optimum movement of loading the tray inside the device to the necessary positions: ...

  • Page 33: Operation With Ipextract

    InnuPure C16 touch Operation with IPextract Operation with IPextract Overview of IPextract All IPextract program functions can be accessed via the H page. start page Fig. 9 page The H page appears after starting IPextract. Via the menus displayed on this page, all basic functions of IPextract can be accessed: Functions Creating a new extraction template...
  • Page 34 Operation with IPextract InnuPure C16 touch The following icons/functions are frequently used in the program: Frequently used icons Icon Function Call up help. Confirm change/input/selection. Cancel change/input/selection. Start T tool. RAY OUT The sample tray holder is ejected and retracted for loading and unloading (→ “Checking and executing individual device functions with tools”...

  • Page 35: Creating And Loading Templates

    InnuPure C16 touch Operation with IPextract Creating and loading templates 1. Open the H start page. Creating a new template 2. Select the menu item T  N  K EMPLATES EMPLATE BASED  The Template window for entering the extraction parameters appears (→ Ed- iting and saving extraction parameters p.

  • Page 36: The General Page - Entering General Extraction Run Options

    Operation with IPextract InnuPure C16 touch Overview of T EMPLATE window Fig. 10 Overview of template screen Element Description Headline Information on the name of the template or project file and the button to locking the touch function of the screen Screen selection Selection of the template page Template screen...

  • Page 37: The Kit Information Page - Entering Kit Data

    InnuPure C16 touch Operation with IPextract Field Description Shows date and time of the creation of the template This data is automatically adopted from the operating system of the tablet. Additional information on the extraction run OMMENTS 6.3.2 The Kit Information page - Entering kit data Enter the kit data on the K page.
  • Page 38 Operation with IPextract InnuPure C16 touch Element Description The description of the selected kit is displayed. ESCRIPTION Enter the expiry date of the kit (see packaging of the kit) using the date for- XPIRY mat MM/YYYY, e.g. 12/2017. This entry is mandatory. It is checked whether the expiry date has been ex- ceeded.

  • Page 39: The Extraction Page - Selecting The Extraction Protocol

    InnuPure C16 touch Operation with IPextract 6.3.3 The Extraction page – selecting the extraction protocol The extraction page serves to select the lysis type and the extraction protocol used. Please refer to the kit manual for information on the suitable protocol and the lysis. Fig.

  • Page 40: Sample Layout Page - Entering The Sample Information

    Operation with IPextract InnuPure C16 touch 6.3.4 Sample Layout page – entering the sample information The sample information is entered on the S page. Inputs on this page are AMPLE AYOUT optional. Sample information can be edited in either the graphical or the spreadsheet view. Editing sample infor- mation in the diagram Fig.
  • Page 41 InnuPure C16 touch Operation with IPextract 5. Enter the sample name in the N field or scan it with the barcode reader. 6. Enter the sample quantity in the Q field and select the measurement unit UANTITY from the list. 7.

  • Page 42: The Monitoring Page - Parameter Overview / Saving Templates / Starting An Extraction Protocol

    Operation with IPextract InnuPure C16 touch Notes on data entry:  When confirming an entry with ENTER, the cursor moves to the next active (occu- pied) field below. In the last line the cursor remains in the field, the entered value is accepted.

  • Page 43: Executing An Extraction Protocol

    InnuPure C16 touch Operation with IPextract Fig. 18 page ONITORING Saving a template 1. Tap on after entering all parameters on the T window. EMPLATE 2. Select the storage medium: T PC or USB M ABLET EMORY TICK 3. Enter the file name in the S field.

  • Page 44: Starting The Extraction Protocol

    Operation with IPextract InnuPure C16 touch 6.4.1 Starting the extraction protocol A template containing the following information is required to start an extraction run: Starting prerequisites  Data of the used kit (→ The Kit Information page - Entering kit data p. 37) ...

  • Page 45: Locking The Touchscreen

    InnuPure C16 touch Operation with IPextract  The currently performed step of the extraction process is interrupted. 2. Tap again on  The extraction process is continued. Stopping the extraction 1. Tap on on the M page. ONITORING  The extraction process is stopped and cannot be continued later. 2.

  • Page 46: Opening A Project

    Operation with IPextract InnuPure C16 touch 3. Select the file name in the S field. AVE AS 4. Tap on  The project is stored. For the next extraction protocol, a new template has to be created or loaded. In addi- tion, it is possible to extract a template from the project (→...
  • Page 47 InnuPure C16 touch Operation with IPextract Fig. 20 Loaded project with log data on the M page ONITORING Like the template, a project can also be exported on the M page as a PDF. Exporting the project as ONITORING The export can take place immediately after running of an extraction protocol or after opening a saved project.

  • Page 48: Checking And Executing Individual Device Functions With Tools

    Operation with IPextract InnuPure C16 touch Checking and executing individual device functions with tools Individual device functions can be executed and checked using special programs (tools). These tools allow, for example, for the filter tips to be emptied and ejected af- ter abortion of an extraction protocol.

  • Page 49: General Settings In Ipextract

    InnuPure C16 touch Operation with IPextract General Settings in IPextract Select the menu item O  G on the start page H for general settings in PTIONS ENERAL IPextract. Fig. 21 page with general settings ENERAL The page G includes following functions: ENERAL ...

  • Page 50: Selecting The Display Of Available Extraction Protocols

    Operation with IPextract InnuPure C16 touch 6.7.2 Selecting the display of available extraction protocols In the default settings, only the Kits with the same lysis type (internal or external) are available for the multi kit use (option M on the the K type).

  • Page 51: Managing User-Defined Sample Types

    InnuPure C16 touch Operation with IPextract 6.7.5 Managing user-defined sample types User-defined sample types are managed in this tool. In the template, entered sample types also appear in the sample type management. 1. Select the menu item O  G on the start page H PTIONS ENERAL...

  • Page 52: Selecting The Time Zone / Adapting Daylight

    Operation with IPextract InnuPure C16 touch 6.7.6 Selecting the time zone / adapting daylight You can select the system time according to the local time zone. The time zone is se- lected on the G page. ENERAL 1. Select the menu item O ...
  • Page 53 InnuPure C16 touch Operation with IPextract Fig. 23 page ANAGEMENT Icons Icon Function Create a new folder Rename a folder/file Delete a folder/file Cut a folder/file The folder/file is copied to the clipboard and removed from its original storage lo- cation.
  • Page 54 Operation with IPextract InnuPure C16 touch 1. Tap on the folder or file. Renaming files/folders 2. Tap on 3. Enter the new name. 4. Tap on  The folder or file is now displayed with the new name. 1. Tap on the folder or file. Deleting a folder/file 2.

  • Page 55: Maintenance And Care

    InnuPure C16 touch Maintenance and care Maintenance and care The InnuPure C16 touch is mainly maintenance-free. Care and maintenance are lim- ited to cleaning the device, changing the fuses and installing software updates. WARNING Danger of electric shock! The device cover must only be opened by the technical customer service of Analytik Jena or its authorized agency.
  • Page 56 Maintenance and care InnuPure C16 touch These are only to be used for wiping disinfection. If using a spray head, first apply the disinfection agent on suitable cloths.  We recommend the following disinfection agent for the front screen made of acrylic glass and for the Decosept Special by Dr Schuhmacher GmbH...

  • Page 57: Exchanging Fuses

    InnuPure C16 touch Maintenance and care Exchanging fuses WARNING Danger of electric shock! Disconnect the mains plug from the mains connection at the rear prior to exchanging fuses! 1. Disconnect the mains plug from the connection at the rear of the device. 2.

  • Page 58: Fault Removal

    Fault removal InnuPure C16 touch Fault removal Attention If faults cannot be remedied by the customer, the Analytik Jena service department or agency must always be informed. This also applies for the repeated occurrence of indi- vidual faults. In case of an error message, perform the following steps: ...

  • Page 59: Transport And Storage

    InnuPure C16 touch Transport and storage Transport and storage Transport 9.1.1 Preparing the device for transport WARNING Risk of damage to health due to improper decontamination! Perform a professional and documented decontamination of the device before return- ing it to Analytik Jena. The decontamination protocol is provided by the service when the return is registered.
  • Page 60 Transport and storage InnuPure C16 touch To remove the brackets, slide the tool under the up curved side of the bracket and carefully lift the bracket. While doing so, hold the bracket with one hand! CAUTION! Risk of injury! If you do not hold the bracket, it may jump away and cause injuries! Assemble the side panels with the arrows of the printed sym- bols pointing upwards.
  • Page 61 InnuPure C16 touch Transport and storage 6. Insert the sample tray (1), the priming station (2) and the adapters for reagent strips (3) (optional) into the spaces in the accessory box provided. If there are no adapters (3), fill the empty spaces with the suitable foam parts.
  • Page 62 Transport and storage InnuPure C16 touch 12. Slide the second foam frame for the device top onto the In- nuPure C16 touch. 13. Place the black foam wedge in front of the plexiglas front screen to protect it against scratches (see arrow). 14.

  • Page 63: Transport Notes

    InnuPure C16 touch Transport and storage 19. Mount the two missing side panels. 20. Close the transport box with the lid. 21. Screw the tool required to remove the brackets on the transport box. 9.1.2 Transport notes Observe the safety instructions in chapter “Safety instructions, transport and installa- tion”...

  • Page 64: Storage

    Transport and storage InnuPure C16 touch Storage Attention Environmental influences and condensate formation can destroy individual compo- nents of the device! The device must only be stored in air-conditioned rooms. The atmosphere must be low in dust and free from aggressive vapors. If the device is not installed immediately after delivery or not required for prolonged periods, it should be stored in its original packaging.

  • Page 65: Disposal

    InnuPure C16 touch Disposal Disposal At the end of its service life the InnuPure C16 touch and all its electronic components must be disposed of in accordance with the applicable regulations as electronic waste.

  • Page 66: Specification

    Specification InnuPure C16 touch Specification 11.1 Technical data System parameters Extraction principle Based on surface-functionalized magnetic or paramagnetic parti- cles or based on the Smart extraction principle Extraction duration < 45 min (without lysis) < 100 min (with lysis) MAG kits yield Depending on the sample type and quantity ...
  • Page 67 InnuPure C16 touch Specification Elution Separate storage tubes with lid Elution volume Depending on the protocol from 20-500 µl adjustable in 10 µl steps Accessories and kits Sample tray Carrier for the pre-insertion of all consumables and samples Priming station Rack for easy preparation of the sample tray Kits DNA extraction and RNA extraction from different source materi-...

  • Page 68: 11.2 Guidelines And Standards

    Specification InnuPure C16 touch 11.2 Guidelines and standards The InnuPure C16 touch is protection class I. The casing is rated IP 20. Protection class and type of protection The InnuPure C16 touch complies with the safety standards Device safety  DIN EN 61010-1 ...

  • Page 69: Ec Declaration Of Conformity

    InnuPure C16 touch EC Declaration of Conformity EC Declaration of Conformity...

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