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Related Manuals for AFi LOREENA
Summary of Contents for AFi LOREENA
- Page 1 User Manual Revision 1.3 English Version...
- Page 2 601997 This document has been prepared with the utmost care. However, AFI Centrifuge refuses to accept any responsibility in the event of errors or omissions. The same applies to any damage resulting from the use of information contained in this manual.
- Page 3 AFI-C300V AFI –C300R AFI-C300RF AFI –C300V-E AFI –C300R-E AFI-C300RF-E Operation Manual (115 VAC, 60 Hz) (230 VAC, 50 Hz) Manufactured by: AFI Centrifuge Rue N. Copernic ZI Nord Bazouges 53200 CHATEAU-GONTIER FRANCE Originale Instructions- Rev.1.3 User Maunal– Loreena 1/ 68...
- Page 4 4.6, 4.7, 4.9, 4.10, August, 2018 F. LE CAM new shortcuts, "routine" program, End of 4.11, 0, 4.14, 9 cycle Flashing signal, information menu modifications; Add new rotors & rotor specifications modifications. Originale Instructions- Rev.1.3 User Maunal– Loreena 2/ 68...
- Page 5 Claims under this warranty should be directed to AFI Centrifuge. setting forth in detail the nature of the defect, the date of the initial installation and the serial and model number of the equipment.
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Page 6: Table Of Contents
2.3........................... 14 LECTRICAL ENVIRONMENT 2.4............................... 14 NPACKING 2.5..............................14 ACKING LIST 2.6..............................15 ESCRIPTION LOREENA - AFI-C300 ------------------------------------------------------------------- 16 PERATING THE CENTRIFUGE 3.1............................. 16 IRST TART OWERING 3.2. ) ..........................17 EVELLING LOOR MODEL 3.3. - Page 7 OTOR 9.6. NU-RA30-15 ..........................63 NGLE OTOR 9.7. NU-RA16-5 ..........................64 NGLE OTOR 9.8. NU-RA48-2 ..........................65 NGLE OTOR 9.9. NU-RA30-2 ..........................66 NGLE OTOR 10. EC C -------------------------------------------------------------------------------------------- 67 ONFORMITY ECLARATION Originale Instructions- Rev.1.3 User Maunal– Loreena 5/ 68...
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Page 8: General Information
LOREENA Centrifuges 1.2. Intended use The centrifuge is designed and manufactured for use with rotating accessories supplied by AFI Centrifuge. It is suitable for separating substances of different densities held in suspension, or emulsion in a liquid. The maximum density of substances should be 1.2 g/ml. -
Page 9: Prohibitions
Rotating rotors and accessories that are not maintained according to the instructions in this manual, or showing signs of wear and/or corrosion. Rotating rotors and accessories not supplied by AFI Centrifuge. Explosive environments Radioactive environments Only operate a centrifuge after taking all necessary safety measures. -
Page 10: Models, Specifications And Accessories
Integrated systems Imbalance detection sensor Motorized lid lock Rotor speed control Control System Microprocessor Acceleration slopes Braking slopes Speed control precision ± 10 rpm Number of programs 99 + Pre-cooling + Pre-warming Originale Instructions- Rev.1.3 User Maunal– Loreena 8/ 68... -
Page 11: Accessories
Set of 4 Adapters : AFI-3T750F Set of 4 750 ml Flat Bottle 4 x 750 ml AFI-3T625F 625 ml Flat Bottle (AFI) 4 x 525 ml Set of 4 AFI-3T500F Set of 4 500 ml Flat Bottle 4 x 500 ml... - Page 12 Tube of grease 20gr One unit Angular Rotor NU-RA6-100 6 x 100 ml One unit Adapters AFI-100R80-OAK 6 x 80ml 80ml AOR Advanced Oak Ridge 6 x 80ml Set of 6 AFI-100R50-C 6 x 50ml 50ml Conical Tube 6 x 50ml...
- Page 13 48 x 2 ml Rotor NU-RA30-2 Angular Rotor 30 x 2 ml Adapters AFI-30R05 0,25ml MicroTube 30 x 2 ml AFI-30R025 0,2ml MicroTube 30 x 2 ml AFI-30R02 0,5ml MicroTube 30 x 2 ml Originale Instructions- Rev.1.3 User Maunal– Loreena 11/ 68...
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Page 14: Safety Instructions
Check the condition of the tubes and remove damaged tubes. Use only the rotors and accessories authorized by the manufacturer AFI Centrifuge. Swing-out rotors: install four (or two) buckets; never perform any cycle with missing buckets. ... -
Page 15: Installation Instructions
However, damage can occur in any shipment and the following outlines the steps you should take on receipt of a LOREENA Centrifuge to be sure that if damage has occurred, the proper claims and actions are taken immediately. -
Page 16: Electrical Environment
For floor model centrifuge, let a minimum of 1 meter space behind the wooden ramp lied on the floor before getting down the device. The LOREENA Centrifuge was packed with great care to prevent transport hazards. Before disposing of the packaging of the centrifuge, and its spinning accessories, please make sure you are in possession of the following: 2.5. -
Page 17: Description
3) Control panel 4) Electronic warhead 5) Main switch 6) Cooling unit (Refrigerating model only) 7) Electrical outlet Fig. 1 Refrigerating centrifuge 8) Wheels (x4) 9) Adjustable pads (x2) Fig. 2 Floor centrifuge Originale Instructions- Rev.1.3 User Maunal– Loreena 15/ 68... -
Page 18: Operating The Loreena Centrifuge - Afi-C300
3. Operating the LOREENA centrifuge - AFI-C300 3.1. First Start / Powering The first start must fulfill the conditions of the IEC 61010-2-020 safety standard HOW TO GREASE A LOREENA CENTRIFUGE Originale Instructions- Rev.1.3 User Maunal– Loreena 16/ 68... -
Page 19: Levelling (Floor Model)
Swing-out rotors are used with round buckets or micro titration buckets. Place the buckets in the corresponding numbered locations. 3.5. First powering Activate the switch on the bottom right of the control center. On/Off Switch position Under Control Center Originale Instructions- Rev.1.3 User Maunal– Loreena 17/ 68... -
Page 20: Using The Interface
Excessive imbalance is likely to damage the turning components and/or the centrifuge. Loading the buckets Allowed: Forbidden : Loading the rotor Allowed: Forbidden: Symmetrical loading Incomplete loading Asymmetrical loading Originale Instructions- Rev.1.3 User Maunal– Loreena 18/ 68... -
Page 21: Quick Start-Up
3.9. Quick start-up Before using the centrifuge, make sure it has been properly installed. Originale Instructions- Rev.1.3 User Maunal– Loreena 19/ 68... -
Page 22: Operating
To exit the menu without saving changes: Enter the program number you wish to create Confirm saving Note: Accept without saving changes: The program has been saved. (The lock is not activated). Originale Instructions- Rev.1.3 User Maunal– Loreena 20/ 68... -
Page 23: Set Program Settings
The new set value appears under the pictogram. Note : In the case of a temperature setpoint, it is possible to set a negative value using the key opposite. The minimum value is -10 ° C. Originale Instructions- Rev.1.3 User Maunal– Loreena 21/ 68... -
Page 24: Set Speed In Rpm Or Rcf
Note : The "…" icon leads to accurate radius setting menu. Press onto arrows to access to angular rotors. Enter the RCF (g) value desired, Confirm The program will now display the RCF value (g). Originale Instructions- Rev.1.3 User Maunal– Loreena 22/ 68... -
Page 25: Protect The Settings Of A Program
Turn on the centrifuge. The switch is in the lower right portion. Any user can access a program (user, admin, service tech) Visual Instruction Control Select the program you want to use Load the rotor and buckets as instructed in § 3.2 Close the lid Start the cycle Originale Instructions- Rev.1.3 User Maunal– Loreena 23/ 68... -
Page 26: Interrupt Centrifugation
Turn off the power by pressing the switch to the “0” position. Insert the wrench provided fully into the round cavity on the left side. Turn it clockwise to release the lid. Remove the wrench. Unload samples. Originale Instructions- Rev.1.3 User Maunal– Loreena 24/ 68... -
Page 27: Precooling
On the Refrigerating model, a "Pre-warming" cycle prior to centrifugation allows to warm the centrifuge so it is ready for a protocol at ambient temperature or warmer (especially after a cycle at a colder temperature). Visual Instruction Control Originale Instructions- Rev.1.3 User Maunal– Loreena 25/ 68... -
Page 28: Routine Menu
Select the program "0" or "...". It is possible to modify the parameters. If no backup is performed, the settings will not be saved when the power is turned off. 4.12. Other menus Visual Instruction Control Originale Instructions- Rev.1.3 User Maunal– Loreena 26/ 68... - Page 29 Access to other menus by pressing : Select the menu User Preferences Service menu Information Go back to the centrifugation screen. Originale Instructions- Rev.1.3 User Maunal– Loreena 27/ 68...
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Page 30: User Preferences
Keyboard type Background Color End of Cycle Alarm Error Message Alarm Automatic lid opening Sleep mode Post Cool Pre-Cool Function Temperature Display Mode Originale Instructions- Rev.1.3 User Maunal– Loreena 28/ 68... -
Page 31: Type Of Speed Displayed (Rpm Or Rcf)
Select the unit you want displayed by default: RPM: Rotation Per Minute RCF: Relative Centrifugal Force, or G Force Confirm Do not turn the centrifuge off at this point You now have to restart the unit. Originale Instructions- Rev.1.3 User Maunal– Loreena 29/ 68... -
Page 32: Frequency Of Lubrication
The messages are recorded per error number, making it easier to save all the errors and how often they happen. For more details on these error codes, refer to section 6.2.22. Return Originale Instructions- Rev.1.3 User Maunal– Loreena 30/ 68... -
Page 33: Type Of Temperature Displayed
Select the type of timer: ON: The timer only starts when the centrifuge has reached the set speed in the program OFF: The timer starts right when you start the cycle (Press Confirm Originale Instructions- Rev.1.3 User Maunal– Loreena 31/ 68... -
Page 34: Counting Mode
This function is used to set keyboard type. Select keyboard type : QWERTY. AZERTY: This is the default setting. QWERTY / AZERTY keyboards Keyword capital letters Access numbers & special characters. Originale Instructions- Rev.1.3 User Maunal– Loreena 32/ 68... -
Page 35: Change Background Color
ON : The lid automatically opens at the end of a cycle. OFF : At the end of the cycle, the lid opens only after pressing the lid opening button. This is the default setting. Validate Originale Instructions- Rev.1.3 User Maunal– Loreena 33/ 68... -
Page 36: Sleep Mode
OFF: Bowl temperature is not maintained between runs. Validate When Post Cool is activated this symbol appears: On the main screen next to the thermometer. On the Precool screen right of the temperature. Originale Instructions- Rev.1.3 User Maunal– Loreena 34/ 68... -
Page 37: Pre Cool Function
During Centrifugation: If the Rotor mode is selected the tube appears. If the Air mode is selected no logo appears. Pressing the temperature button can temporarily change the display mode. Originale Instructions- Rev.1.3 User Maunal– Loreena 35/ 68... -
Page 38: Service Menu
The "Postcool" function makes it possible to maintain a temperature in the chamber of the tank, after the rotor has stopped. Stopping this function prevents over-consumption of electricity. This function is disabled ("OFF") by default. Originale Instructions- Rev.1.3 User Maunal– Loreena 36/ 68... -
Page 39: Care And Maintenance
Check that the air vents are not obstructed. Centrifuge chamber: Remove the rotor before cleaning the tank. Dry thoroughly after cleaning. Do not use fluffy fabric. Replace the rotor after cleaning the tank. Originale Instructions- Rev.1.3 User Maunal– Loreena 37/ 68... -
Page 40: Rotor & Accessories
Bio hazardous, radioactive and/or toxic materials: Before any intervention, to ensure the safety of the operator, please refer to the laboratory’s decontamination protocol. The centrifuge and its accessories may be contaminated and require remediation in this case. Originale Instructions- Rev.1.3 User Maunal– Loreena 38/ 68... -
Page 41: Dismantling The Rotor
“click” sound, the rotor is unlocked 1. 3.Grab and lift the rotor with both hands once it’s unlocked. Set the rotor on a flat surface or on one of AFI Centrifuge’s rotor stand. 5.3. Limiting the duration of operation The use of accessories and the rotors is limited in time to ensure the integrity of their mechanical strength. -
Page 42: Checking And Testing Accessories
Check that the grounding of the device is always properly secured by its power cable and the wall outlet it is connected to. The operator is not authorized to access the internal part of the equipment. An AFI Centrifuge authorized technician will ensure maintenance. -
Page 43: Unbalancing Sensitivity Detection
The centrifuge has a reaction to the load balancing faults which may be different depending on the media on which the centrifuge is placed. A calibration of the imbalance sensitivity is therefore necessary during installation. Required equipment: Use the reference balancing kit AFI-71122002. Visual... -
Page 44: Error Messages, Troubleshooting, Option-Diagnostics
Let the centrifuge cool down between cycles. (ventilated model). The motor has been damaged Contact a Certified Technician. The temperature sensor on the motor has a Contact a Certified Technician. bad connection. Originale Instructions- Rev.1.3 User Maunal– Loreena 42/ 68... - Page 45 In case of malfunction, contact after-sales service if the previous table does not eliminate the error. You will be asked for the type of centrifuge and the serial number for more effective troubleshooting. Originale Instructions- Rev.1.3 User Maunal– Loreena 43/ 68...
- Page 46 Never try to disassemble or fix the unit on your own: the risks of getting injured or electrocuted are high. The manufacturer warranty would then be void. Only a certified technician is authorized to perform such operations. Originale Instructions- Rev.1.3 User Maunal– Loreena 44/ 68...
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Page 47: Wearing Parts & Consummable
5.10. Back to manufacturer In case of a need to return to AFI Centrifuge manufacturer of the device and / or its accessories, all elements must be decontaminated before transport, in order to protect the various parties involved and the environment. -
Page 48: Electrical/Environmental Requirements
In the documentation for the user, a statement shall be included drawing WARNING attention to the fact that there may be potential difficulties in ensuring electromagnetic compatibility in other environments, due to conducted as well as radiated disturbances. Originale Instructions- Rev.1.3 User Maunal– Loreena 46/ 68... -
Page 49: Disposal And Recycle
Lead Free Electronic Wire PVC Coated Copper Connectors Nylon Hardware Stainless Steel and Steel Note: Material type can be verified with use of a magnet with stainless and aluminum being non-magnetic. Originale Instructions- Rev.1.3 User Maunal– Loreena 47/ 68... -
Page 50: Theoretical Basics Of Centrifugation
1000 Integral spin It is the accumulated relative centrifugal force acting on the centrifuged object at the corresponding time. It is outlined by the colored surface of the chart below. Duration Originale Instructions- Rev.1.3 User Maunal– Loreena 48/ 68... -
Page 51: Nomogram
The nomogram graphically connects by a line segment a speed to the relative centrifugal force (RCF) as a function of the radius of rotation selected. In the example above, the speed of 2500 rpm corresponds to a relative centrifugal force of 550 g for a radius of rotation of 79 mm Originale Instructions- Rev.1.3 User Maunal– Loreena 49/ 68... -
Page 52: Logbook
8.3. Logbook Example of table to monitor the use of the centrifuge and accessories. Date Operator Duration T° Rotor Incidents Originale Instructions- Rev.1.3 User Maunal– Loreena 50/ 68... -
Page 53: Return Coupon
Site Address Post code Town Country Phone Email Address Billing address (If different from the site Town address) AFI Centrifuge Contact information Society AFI CENTRIFUGE Address : ZI Nord Bazouges Post code 53200 Town Château-Gontier Description of your equipment Designation Ref. -
Page 54: Annex 1: Accessory Specifications
75023x Tubes 5/7 ml 4750NU-2T5 Round bottom 196182 mm 62523x Tubes 5/7 ml 4625NU-2T5 Round bottom 196182 mm 50023x Tubes 5/7 ml 4500CNU-2T5 Round bottom 290F 183182 mm 229023x Tubes 5/7 ml Round bottom Originale Instructions- Rev.1.3 User Maunal– Loreena 52/ 68... - Page 55 Maximum speed 3500 rpm Maximum RCF 2655 xg Microplate capacity 6 x 96 wells Deepwell block capacity 1 x 96 wells Maximum capacity : 800 grams Lifetime 50,000 cycles (Indicative duration) 8 years Originale Instructions- Rev.1.3 User Maunal– Loreena 53/ 68...
- Page 56 (portoirs & tubes (inserts & loaded tubes) 2xNU-BXDW4 empty With empty inserts. pleins) 2xNU-B1000 empty buckets (ex: without insert) 2 nacelles NU- buckets (ex: without BXDW4 chargés insert) Symmetrical loading Asymmetrical loading Incomplete loading Originale Instructions- Rev.1.3 User Maunal– Loreena 54/ 68...
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Page 57: Swing-Out Rotor Nu-Rx750
1x 625 ml Bottle … mm Flat bottom 1x 400 ml Bottle AFI-3T400F 198 mm Flat bottom 1x 290 ml Bottle AFI-3T290F 198 mm Flat bottom 1x 250 ml Bottle AFI-3T250C 200 mm Conical bottom Originale Instructions- Rev.1.3 User Maunal– Loreena 55/ 68... - Page 58 21x Tubes 10 ml Blood Collection AFI-3T10 198 mm Tubes 21x Tubes 14 ml Urine Tubes 27x 5-7 ml AFI-3T5 197 mm Blood Collection Tubes 2x57x MicroTubes AFI-3T2 198 mm 1.5 / 2 ml Originale Instructions- Rev.1.3 User Maunal– Loreena 56/ 68...
- Page 59 Maximum capacity : 800 grams Lifetime 50,000 cycles (Indicative duration) 8 years Maximum Radius : 193mm Included : Part number : NU-TXDW3 Description: Stainless Steel Extractor & Neoprene pad (Set of 4) Originale Instructions- Rev.1.3 User Maunal– Loreena 57/ 68...
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Page 60: Swing-Out Rotor Nu-Rhdw
Stainless Steel Extractor (set of 2) Part number: NU-LH Description : Airtight lid for Microplate (Set of 2) Configurations: Loading the buckets Allowed: Not allowed: Loading the rotor: Forbidden : Allowed: Incomplete loading Symmetrical loading Asymmetrical loading Originale Instructions- Rev.1.3 User Maunal– Loreena 58/ 68... -
Page 61: Angular Rotor Afi-Rhd3
AFI-RHD3 Description: Swinging Bucket Rotor Maximum capacity: 2x 3 kg Fastening: ClickSpin system Maximum rotor speed 3700 rpm (With AFI-BHD3 carriers) Maximum centrifugal force of 2510 xg (With AFI-BHD3 carriers) the rotor 30 000 cycles Lifetime (6 years) Maximum speed... - Page 62 Configurations: Loading the buckets Allowed: Not allowed: Loading the rotor: Forbidden : Allowed: Incomplete loading Symmetrical loading Asymmetrical loading Originale Instructions- Rev.1.3 User Maunal– Loreena 60/ 68...
- Page 63 Angular rotor NU-RA24-2 Originale Instructions- Rev.1.3 User Maunal– Loreena 61/ 68...
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Page 64: Angle Rotor Nu-Ra8-50
12100 rpm Maximum centrifugal force of the 16660 xg rotor Maximum Maximum Centrifuge performance speed centrifugal force AFI-C300 Ventilated 13000 rpm 19272 xg AFI-C300 Refrigerating 13500 rpm 20783 xg Maximum radius 102 mm Originale Instructions- Rev.1.3 User Maunal– Loreena 62/ 68... -
Page 65: Angle Rotor Nu-Ra30-15
Maximum radius : 140 mm (without cups) Configurations : Loading the rotor The rotor must be loaded evenly. Allowed Forbidden: A load with number of odd tubes is prohibited for this rotor. Originale Instructions- Rev.1.3 User Maunal– Loreena 63/ 68... -
Page 66: Angle Rotor Nu-Ra16-5
107.8 mm Items included: Micro tube rotor airtight lid Accessories for NU-RA16-5 Part number: NU-LM2 Description: Micro tube rotor airtight lid for rotors NU-RA30-2, NU-RA48-2 and NU-RA16-5 Configurations: Loading the rotor Allowed Forbidden: Originale Instructions- Rev.1.3 User Maunal– Loreena 64/ 68... -
Page 67: Angle Rotor Nu-Ra48-2
Micro tube rotor airtight lid for rotors NU- Items included: RA30-2 and NU-RA48-2 Accessories Part number: NU-LM2 Description: Micro tube rotor airtight lid for rotors NU- RA30-2, NU-RA48-2 and NU-RA16-5 Configurations: Loading the rotor Allowed : Forbidden: Originale Instructions- Rev.1.3 User Maunal– Loreena 65/ 68... -
Page 68: Angle Rotor Nu-Ra30-2
Airtight lid for rotors NU-RA30-2 and NU- Items included: RA48-2 Accessories Part number: NU-LM2 Description: Micro tube rotor airtight lid for rotors NU- RA30-2, NU-RA48-2 and NU-RA16-5 Configurations: Loading the rotor Allowed Forbidden: Originale Instructions- Rev.1.3 User Maunal– Loreena 66/ 68... -
Page 69: Ec Conformity Declaration
EC Conformity Declaration Originale Instructions- Rev.1.3 User Maunal– Loreena 67/ 68... - Page 70 Blank page. Originale Instructions- Rev.1.3 User Maunal– Loreena 68/ 68...
- Page 72 3, rue Nicolas Copernic ZA Nord Bazouges 53200 CHATEAU-GONTIER FRANCE Tél : +33(0)2 43 06 66 76 contact@ afigroups.com www.aficentrifuge.com Non-contractual photos. All rights reserved, including photos and illustrations. Copyright ® AFI Centrifuge company – SIRET 513 982 645 00022.
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