AFi SIRENA -C060R User Manual
  1. Manuals
  2. Brands
  3. AFi Manuals
  4. Laboratory Equipment
  5. SIRENA AFI-C060R
  6. User manual

AFi SIRENA -C060R User Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

User Manual
│
Revision 1.0
│
English version

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the SIRENA AFI-C060R and is the answer not in the manual?

Questions and answers

Related Manuals for AFi SIRENA AFI-C060R

Summary of Contents for AFi SIRENA AFI-C060R

  • Page 1 User Manual │ Revision 1.0 │ English version...
  • Page 3 SIRENA CENTRIFUGE Models AFI-C060R AFI-C060R-E User Manual (230 VAC, 50 Hz, 120VAC, 60 Hz) Designed & Manufactured by: AFI Centrifuge Rue N. Copernic ZI Nord Bazouges 53200 CHATEAU-GONTIER FRANCE...
  • Page 4 602363 This document has been prepared with the utmost care. However, AFI Centrifuge refuses to accept any responsibility in the event of errors or omissions. The same applies to any damage resulting from the use of information contained in this manual.
  • Page 5 This warranty shall not apply to any AFI Centrifuge product or part thereof which has been subject to misuse, abuse, accident, shipping damage, improper installation or service, or damage by fire, or flood. If the serial number of this product is altered, removed or defaced as to be illegible, the Warranty shall be null and void in its entirety.
  • Page 6 Explanation of Symbols Safety alert symbol indicates a potentially hazardous situation which, WARNING if not avoided, could result in death or serious injury CAUTION used without the safety alert symbol indicates a potentially hazardous situation which, if not avoided, may result in property CAUTION damage.

  • Page 7: Table Of Contents

    & E ..................... 12 LECTRICAL NVIRONMENTAL EQUIREMENTS 2.9.1 ..........................12 LECTRICAL ENVIRONMENT 2.9.2 ......................... 12 NVIRONMENTAL REQUIREMENTS OPERATING THE SIRENA CENTRIFUGE - AFI-C060 ................... 13 ............................ 13 IRST TART OWERING .............................. 13 OTOR SSEMBLY ............................14 OSITION THE BUCKETS ..............................14 IRST POWERING ............................
  • Page 8 THEORETICAL BASICS OF CENTRIFUGATION.................... 32 ................................32 ASICS ..............................33 OMOGRAM ................................. 33 OGBOOK ............................... 34 ETURN COUPON ROTORS & ACCESSORIES ......................... 35 AFI-RX150 S .......................... 35 WING OTOR AFI-RHDW-150 S ........................40 WING OTOR AFI-RX8-5 S ........................... 41 WING...

  • Page 9: General Information

    The refrigeration system refrigerant used is CFC-free to meet regulations of refrigerant use. Fig. 1 SIRENA Centrifuge Intended use The centrifuge is designed and manufactured for use with rotating accessories supplied by AFI Centrifuge. It is suitable for separating substances of different densities held in suspension, or emulsion in a liquid. CAUTION The maximum density of substances should be 1.2 g/cm...

  • Page 10: Models, Specifications And Accessories

    Models, Specifications and Accessories Specifications Benchtop centrifuge Reference AFI-C060R-E AFI- C060R Nominal voltage (+/- 10%) 230 V 120 V Frequency 50 Hz 60 Hz Max. Current Current phase Max. Power 1100 Watt Dimensions & Weight Dimensions (HxWxD) 29 x 44 x 72 cm 11.4 x 17.3 x 28.4 in...

  • Page 11: Accessories

    150 ml lid for SB150 bucket AFI-LSB150 AFI-L150 150 ml Lid for B150 Swinging Bucket Rotor AFI-RHDW-150 AFI-RX8-5 Swinging Bucket Rotor Adaptors for AFI-BS150 buckets AFI-06T50C-SB 50 ml Conical Tube 50ml AOR* Round Tube AFI-06T50AOR-SB AFI-06T50S-SB 50 ml Skirted Conical Tube 30 ml Conical Tube...
  • Page 12 10ml Blood Collection Tube AFI-50R10 6 x 50 ml Angular Rotor NU-RA6-50 AFI-50R50-OAK 50ml AOR* Round Tube AFI-50R30-C 30ml AOR* Round Tube AFI-50R30-OAK 30ml Conical Tube AFI-50R15-C 15ml Conical Tube 10ml AOR* Round Tube AFI-50R10-OAK AFI-50R10 10ml Blood Collection Tube...

  • Page 13: Safety Instructions

     Check the condition of the tubes and remove damaged tubes.  Use only the rotors and accessories authorized by the manufacturer AFI Centrifuge.  Swing-out rotors: install four (or two) buckets; never perform any cycle with missing buckets. ...

  • Page 14: Installation Instructions

    2 Installation Instructions Shipment AFI Centrifuge takes every reasonable precaution to assure that your ISA Centrifuge arrives without damage. Motor carriers are carefully selected and shipping cartons have been specially designed to insure your purchase. However, damage can occur in any shipment and the following outlines the steps you should take on receipt of a ISA Centrifuge to be sure that if damage has occurred, the proper claims and actions are taken immediately.

  • Page 15: Machine Description

    Machine Description Window Power switch button Control panel Opening lid button Cooling unit Electrical outlet Fig. 1 ¾ Front view Interface description 1. Power ON-OFF, START-STOP, & cycle interruption button. Can be activated through single press or press & hold action. 2.

  • Page 16: Electrical & Environmental Requirements

    Electrical & Environmental Requirements The centrifuge requires must be 230V/50Hz or 120V/60Hz, single phase, (current rating varies per centrifuge model) according to the manufacturer sheet on the back of the unit. The centrifuge must be plugged into an outlet with protective earthing connection with the standard power cord. The electrical outlet into which the centrifuge is connected should be readily accessible for maintenance purposes.

  • Page 17: Operating The Sirena Centrifuge - Afi-C060

    3 Operating the SIRENA centrifuge - AFI-C060 First Start / Powering The first start must fulfill the conditions of the IEC 61010-2-020 safety standard HOW TO GREASE A SIRENA CENTRIFUGE first start, when Grab the synthetic grease container provided with your centrifuge.

  • Page 18: Position The Buckets

    Position the buckets Swing-out rotors are used with round and square buckets or microplate buckets. Place the buckets & lids in the corresponding numbered locations. First powering After transport, wait at least 2 hours before putting the appliance CAUTION into operation so that the refrigerant circuit is fully efficient. Connect the power cord to the mains.

  • Page 19: Rotor Loading And Balancing

    Rotor loading and balancing Position the 4 numbered buckets in their corresponding positions on the rotor. Each bucket should pivot freely in its slot. Place the inserts corresponding to the tubes to be centrifuged in the four buckets. The buckets must be symmetrically distributed. For better balance, each numbered bucket must be placed in the corresponding position marked on the rotor.

  • Page 20: Quick Start-Up

    Quick start-up Before using the centrifuge, make sure it has been properly installed. Original Instructions Rev.1.0 User Manual – Sirena 16/ 56...

  • Page 21: Operating

    4 Operating Settings Program setting is done adjusting successively following parameters: • Speed • Cycle Duration • Temperature • Acceleration Slope • Braking slope Set Speed Action Instruction Effect Select the program number sliding the The program number is displayed under cursor.

  • Page 22: Set Acceleration Slope

    Press touch bar 2x to access to Duration value is blinking. temperature parameter. Modify the blinking value sliding the The value is modified. cursor. Press onto "+" or "-" for accurate The default unit is Celsius degree, setting. symbolized "°C. To modifiy unit, refer to §...

  • Page 23: Use A Program To Centrifuge

    Use a program to centrifuge Select a program Press onto "+" or "-" to select a program number.   Load buckets onto the rotor, following instructions in § § 0 Position the buckets. Close the lid. Press START to begin the cycle. Note : For a "PULSE"...

  • Page 24: Precooling

    Precooling A "Pre-cooling" cycle prior to centrifugation allows to cool the centrifuge down to 4 degrees Celsius so it is ready for temperature sensitive samples. Select program # 0 for Precool program. "Cool" message is displayed. Load buckets onto the rotor, following instructions in § 0 Position the buckets.
  • Page 25 Action Instruction Effect Select the program number sliding the The program number is displayed under cursor. "Prog". Press & hold touch bar twice to access to The selected program is now in Setting parameter. mode. Speed unit is blinking. Press touch bar 1x to access to radius The radius value is now blinking.

  • Page 26: Emperature Nit

    Action Instruction Effect Select the program number sliding the The program number is displayed under cursor. "Prog". Press & hold touch bar 2x to access to Speed unit is blinking. parameter. Press touch bar 3x to access to The Temperature unit value is now Temperature unit.

  • Page 27: Sleep Mode

    Displayed codes are listed below : Displayed code Description Details SOFT Microcontroller software release Imbalance setting (Default value : 350) The higher the value, the bigger imbalance is tolerated. CAUTION CAUTION : Only certified technicians are authorized to adjust this value. Any damage can occur in case of unfollowed instructions.

  • Page 28: Care And Maintenance

    5 Care and Maintenance All maintenance actions on this equipment must be performed by a qualified technician who is familiar with the proper maintenance procedures required for CAUTION this equipment. The device and its accessories may be contaminated. Apply decontamination measures required before any maintenance. CAUTION Turn off the centrifuge power switch.

  • Page 29: Auto Cleaning Function

    71110005. Operating mode : Action Instruction Effect Equip the centrifuge with a AFI-RX- 150 swing-out rotor, and its buckets. Remove the lid circular cover at the When turned 1/3 tour, a beep sound bottom of the cover by turning it indicates that it is not the normal operating manually clockwise.

  • Page 30: Rotor & Accessories

    Use clear or distilled water to clean the rotor and buckets. A soft non-metallic brush or lint-free cloth can be used. Dry the rotor and accessories ensuring the least accessible areas are wiped. Image Operation To ensure a proper swinging movement of the buckets, clean all 8 trunions of the rotor.

  • Page 31: Dismantling The Rotor

    Check that the grounding of the device is always properly secured by its power cable and the wall outlet it is connected to. The operator is not authorized to access the internal part of the equipment. An AFI Centrifuge authorized technician will ensure maintenance.

  • Page 32: Replacing The Battery In The Lid

    1) Remove the black plastic cap in lower right corner of the lid. 2) Remove carefully the battery from its housing. 3) Replace the battery with a new CR2032 battery. 4) Replace the plastic cover to its original position.. 5) To check the operation of the battery, turn the circular lid cover 1/3 turn counterclockwise.
  • Page 33 POWER BOARD The power board is working improperly. Contact a Certified Technician. SECURITY DEFAULT The connection to this board parts is not Contact a Certified Technician to check good. (P1, P3, P4, P5 pins). NO SPEED SIGNAL No speed measurement within 5s after the The speed sensor or the connection of AT START start of the centrifuge The speed sensor is...

  • Page 34: Reminder Messages

    Back to manufacturer In case of a need to return to AFI Centrifuge manufacturer of the device and / or its accessories, all elements must be decontaminated before transport, in order to protect the various parties involved and the environment.

  • Page 35: Disposal And Recycle

    6 Disposal and Recycle Before disposing the equipment, it must be decontaminated and cleaned to protect people, the environment and equipment. Legal regulations must be observed when disposing of the unit. Electrical appliances are governed by national regulations based on the 2002/96/EC European Directive on electrical and electronic equipment waste WEEE.

  • Page 36: Theoretical Basics Of Centrifugation

    7 Theoretical Basics of Centrifugation Basics Relative Centrifugal Force In customary practices and usages of centrifugation, the quantization unit of angular velocity (denoted N), is the revolution per minute (abbreviated rpm, RPM, r / min, or r • min-1). This is a measure of frequency of rotation. It corresponds to the number of full rotations completed in one minute around a fixed axis.

  • Page 37: Nomogram

    Nomogram RCF (Number of Radius (r/min) grams) (mm) The nomogram graphically connects by a line segment a speed to the relative centrifugal force (RCF) as a function of the radius of rotation selected. In the example above, the speed of 2500 rpm corresponds to a relative centrifugal force of 550 g for a radius of rotation of 79 mm Logbook Example of table to monitor the use of the centrifuge and accessories.

  • Page 38: Return Coupon

    Site Address Post code Town Country Phone Email Address Billing address (If different from the site Town address) AFI Centrifuge Contact information Society AFI CENTRIFUGE Address : ZI Nord Bazouges Post code 53200 Town Château-Gontier Description of your equipment Designation Ref.

  • Page 39: Rotors & Accessories

    260 g Lifetime 500 000 cycles (>20 years) Maximum Radius : 151 mm Part number: AFI-L150 Description : Lid for AFI-B150 bucket (Set of 4) Inserts (Sets of 4) Part Number Radius (*) Capacity per insert Rotor Total capacity Visual AFI-06T100R...
  • Page 40 1x 50 ml Skirted AFI-06T50S 149 mm Conical Tube 1x 30 ml Conical AFI-06T30C 151 mm Tube 1x 30 ml Skirted AFI-06T30S 149 mm Conical Tube 2x 15ml Conical AFI-06T15C 150 mm Tube 3x 10ml Conical AFI-06T10C 149 mm Tube...
  • Page 41 500 000 cycles (>20 years) Maximum Radius : 151 mm Part number: AFI-LSB150 Airtight lid (Set of 4) Description : For AFI-SB150 Square bucket Inserts (Sets of 4) Radius (*) Capacity per insert Rotor Total capacity Visual Part Number 100 ml Tube...
  • Page 42 4x 10ml AFI-06T10C-SB 149 mm Conical Tube 6x 10ml Blood Collection AFI-06T10-SB 149 mm Tube 14ml Urine Tube 9x 5-7ml AFI-06T5-SB 149 mm Blood Collection Tube 9 x 1,5 - 2ml AFI-06T2-SB 149 mm (2 overstacked MicroTube adapters by bucket)
  • Page 43 Configuration Loading the buckets Allowed: Forbidden : Loading the rotor Allowed: Forbidden: Symmetrical loading Incomplete loading Mixed buckets Asymmetrical loading Original Instructions Rev.1.0 User Manual – Sirena 39/ 56...

  • Page 44: Afi-Rhdw-150 Swing-Out Rotor

    AFI-RHDW-150 Swing-out Rotor Rotor Part number: AFI-RHDW-150 Description: Microplate 150 swing-out rotor Maximum capacity: 2x 4 microplate 96 wells 2x 1,3 kg Fastening: ClickSpin System Maximum rotor speed 5460 rpm (With AFI-BHDW-150 buckets) Maximum centrifugal force 4000 xg of the rotor...

  • Page 45: Afi-Rx8-5 Swing-Out Rotor

    AFI-RX8-5 Swing-out Rotor Rotor Part number: AFI-RX8-5 Description: 0.04 liter swing-out rotor Maximum capacity: 8x5 ml / 8x14 g Fastening: ClickSpin system Maximum rotor speed 13000 rpm Maximum rotor rcf 18 327 xg Lifetime 120 000 cycles (>20 years) Max. Speed on SIRENA...

  • Page 46: Nu-Ra6-100 Angular Rotor

    28° Fastening: ClickSpin system Maximum rotor speed 15000 rpm Maximum centrifugal force 26639 xg Maximum Centrifuge performance Maximum speed centrifugal force AFI-C060 7000 rpm 5807 xg Maximum radius 106 mm Accessories Tubes adapters Total Radius Part number Capacity per insert...

  • Page 47: Nu-Ra8-50 Angular Rotor

    Maximum rotor speed 15 000 rpm Maximum centrifugal force of the rotor 25 658 xg Maximum Maximum Centrifuge performance speed centrifugal force AFI-C060 7500 rpm 6415 xg Maximum radius 102 mm Accessories Tube adaptors Total capacity Part number Radius (*)

  • Page 48: Nu-Ra6-50 Angular Rotor

    ClickSpin system Maximum rotor speed 13000 rpm Maximum centrifugal force of the rotor 19272 xg Maximum Maximum Centrifuge performance speed centrifugal force AFI-C060 9500 rpm 10292 xg Maximum radius 102 mm Accessories Tube adaptors Total Part number Radius (*) Capacity per insert...

  • Page 49: Nu-Ra10-10 Angular Rotor

    ClickSpin system Maximum rotor speed 14600 rpm Maximum centrifugal force of 25023 xg the rotor Maximum Centrifuge performance Maximum speed centrifugal force AFI-C060 8700 rpm 8885 xg Maximum radius 105 mm Airtight lid Item included: Accessories Part number: NU-LM Description:...

  • Page 50: Nu-Fa8-50 Angular Rotor

    Fastening: Integrated Nut Maximum rotor speed 8 700 rpm Maximum Maximum Centrifuge performance speed centrifugal force AFI-C060 5600 rpm 4944 xg Maximum radius 141 mm Items included: 8 x Tube holder & cap Configurations : Loadings A rotor must be uniformly loaded.

  • Page 51: Nu-Ra30-15 Angular Rotor

    Maximum rotor speed 5550 rpm Maximum centrifugal force of the 5062 xg rotor Maximum centrifugal Centrifuge performance Maximum speed force AFI-C060 4700 rpm 3630 xg Maximum radius 147 mm 30x Tube holders Items included: 30x Wells for round bottom tubes Configurations : Loading the rotor The rotor must be loaded evenly.

  • Page 52: Nu-Ra16-5 Angular Rotor

    Maximum rotor speed 16500 rpm Maximum centrifugal force of the 32833 xg rotor Maximum centrifugal Centrifuge performance Maximum speed force AFI-C060 14200 rpm 24347 xg Maximum radius 108 mm Included: Micro tube rotor airtight lid Configurations: Loading the rotor Allowed Forbidden: An even number of tubes spaced evenly in the rotor.

  • Page 53: Nu-Ra48-2 Angular Rotor

    Maximum rotor speed 18 000 rpm Maximum centrifugal force of the 36 585xg rotor Maximum centrifugal Centrifuge performance Maximum speed force AFI-C060 12700 rpm 18213xg Maximum radius 101 mm (outer row) 91 mm (inner row) Included: Micro tube rotor airtight lid Configurations:...

  • Page 54: Nu-Ra30-2 Angular Rotor

    Maximum rotor speed 18000 rpm Maximum centrifugal force of the 35 499 xg rotor Maximum centrifugal Centrifuge performance Maximum speed force AFI-C060 14000 rpm 21475 xg Maximum radius 98 mm Included: Airtight lid Configurations: Loading the rotor Allowed (examples) : Forbidden: An even number of tubes spaced evenly in the rotor.

  • Page 55: Nu-Ra24-2 Angular Rotor

    ClickSpin system Maximum rotor speed 18000 rpm Maximum centrifugal force of the rotor 31 514 xg Maximum Maximum Centrifuge performance speed centrifugal force AFI-C060 18000 rpm 31514xg Maximum radius 87 mm Included: Airtight lid Accessories Part number : NU-SLM Description :...

  • Page 56: Afi-Ra-Pcr-8 X 8 Pcr Rotor

    AFI-RA-PCR-8x8 PCR Rotor Rotor Part number: AFI- RA-PCR8x8 Description: Rotor angulaire 8x 8 ml Maximum capacity: 8x tubes coniques 8/10 ml Maximum load 64 x 10 g Micro tube tilt: 25° Fastening: Système ClickSpin Maximum rotor speed 18000 rpm Maximum centrifugal force of...

  • Page 57: Afi-Ra-H24 Haematocrit Rotor

    AFI-RA-H24 Haematocrit Rotor Rotor Part number: AFI-RA-H24 Description: Haematocrit Rotor Maximum capacity: 24x Capillary Tubes Maximum load Micro tube tilt: 0° Fastening: ClickSpin system Maximum rotor speed 23000 rpm Maximum centrifugal force of 60916 xg the rotor Maximum Centrifuge performance...
  • Page 58 Page left blank voluntarly. Original Instructions Rev.1.0 User Manual – Sirena 54/ 56...

  • Page 59: Declaration Of Conformity

    9 Declaration of Conformity Original Instructions Rev.1.0 User Manual – Sirena 55/ 56...
  • Page 60 Original Instructions Rev.1.0 User Manual – Sirena 56/ 56...
  • Page 61 Page left blank voluntarly.
  • Page 62 Page left blank voluntarly.
  • Page 64 3, rue Nicolas Copernic ZA Nord Bazouges 53200 CHATEAU-GONTIER FRANCE Tél : +33(0)2 43 06 66 76 contact@ afigroups.com www.aficentrifuge.com Non-contractual photos. All rights reserved, including photos and illustrations. Copyright ® AFI Centrifuge company – SIRET 513 982 645 00022.

This manual is also suitable for:

Sirena afi-c060r-e

Table of Contents