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Summary of Contents for AFi KATRINA AFI-C600R-2P
- Page 1 6 x 1000 mL / 12 x 500 mL User Manual │ Revision 1.1 │ English version...
- Page 2 602325 This document has been prepared with the utmost care. However, AFI Centrifuge refuses to accept any responsibility in the event of errors or omissions. The same applies to any damage resulting from the use of information contained in this manual.
- Page 3 KATRINA CENTRIFUGE Models AFI-C600R-2P AFI-C600R-3P Operation Manual (230 VAC ±10%, 50 Hz) Manufactured by: AFI Centrifuge Rue N. Copernic ZI Nord Bazouges 53200 CHATEAU-GONTIER FRANCE Originale Notice - Rev.1.2 User Manual – Katrina p.1/ 55...
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Page 4: Warranty
Claims under this warranty should be directed to AFI Centrifuge. setting forth in detail the nature of the defect, the date of the initial installation and the serial and model number of the equipment. - Page 5 Modification registration Following modifications register updates of the manual. Date Revision Modifications Paragraph Author 2018, sept., 21th Creation F. LE CAM Levelling, Add spare parts F. LE CAM 2018, oct, 3rd 3.1, 5.8 references. Update according to 2020 July 2020 C.
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Page 6: Table Of Contents
: 2.0 ............................ 17 OLLUTION EGREE 2.10.5. C ............................17 HEMICAL XPOSURE 2.10.6. EMC P ) ..................17 ERFORMANCE CLASSIFIED FOR LIGHT INDUSTRIAL KATRINA - AFI-C600R ------------------------------------------------------------------ 19 PERATING THE CENTRIFUGE 3.1................................. 19 EVELLING 3.2............................. 19 IRST TART OWERING 3.3. - Page 7 ECYCLE 6.1................................49 ASICS 6.2..............................50 OMOGRAM 6.3................................51 OGBOOK 6.4..............................52 ETURN COUPON 1: A ------------------------------------------------------------------------------------ 53 NNEX CCESSORY PECIFICATIONS 7.1. AFI-RX6-1000........................53 WING OTOR Originale Notice - Rev.1.2 User Manual – Katrina p.5/ 55...
- Page 8 It includes all the technical specifications of the Katrina device as well as all the necessary information to assist the operator. Before use, the operator must have followed the training course provided by AFI Centrifuge about using the machine and how to achieve optimal results.
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Page 9: General Information
The refrigeration system refrigerant used is CFC-free to meet regulations of refrigerant use. 1.2. Intended use The centrifuge is designed and manufactured for use with rotating accessories supplied by AFI Centrifuge. It is suitable for separating substances of different densities held in suspension, or emulsion in a liquid. -
Page 10: Prohibitions
• Rotating rotors and accessories that are not maintained according to the instructions in this manual, • or showing signs of wear and/or corrosion. • Rotating rotors and accessories not supplied by AFI Centrifuge. • Explosive environments • Radioactive environments CAUTION Only operate a centrifuge after taking all necessary safety measures. -
Page 11: Models, Specifications And Accessories
Modèle / Model Réfrigéré / Refrigerating Centrifugeuse de sol / Floor stand centrifuge Référence / Reference AFI-C600R-2P Tension nominale / Nominal voltage (+/- 10%) 200, 208, 220, 230, 240V - Single phase Fréquence / Frequency 50 Hz Puissance alimentation max. / Power max. - Page 12 Dimensions & poids / Dimensions & Weight Dimensions (HxlxL) / Dimensions (HxWxD) 960 x 800 x 960 mm Dimensions emballage (HxlxL) / 1170 x 900 x 1020 mm Package dimension (HxWxD) Hauteur couvercle ouvert / Opened lid height 1690 mm Poids net hors rotor / Weight unpacked without 352 Kg rotor...
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Page 13: Accessories
71199012 Tube of Grease 20 gr One unit Only equipment listed here or supplied exclusively by AFI Centrifuge can be used. The use of accessories and other cables than those specified can cause malfunction. Originale Notice - Rev.1.2 User Manual – Katrina... -
Page 14: Safety Instructions
• Check the condition of the tubes and remove damaged tubes. • Use only the rotors and accessories authorized by the manufacturer AFI Centrifuge. • Swing-out rotors: install four (or two) buckets; never perform any cycle with missing buckets. •... -
Page 15: Installation Instructions
Unpacking must take place in a sufficiently spacious area: a clearance area of 3 meters in front of the pallet is needed to remove the machine from its packaging. 2 people authorized by AFI Centrifuge are needed to remove the centrifuge from its pallet. -
Page 16: Packing List
2.5. Packing list KATRINA Centrifuge Compliance Certificate User Manual N°6 key Pad clip (Rotor Fixing & Manual release) (Quantity :4) (Quantity :4) Rotor & Rotor Cover Rotor Fixing Nut Food grade grease 1 of the following buckets set : Round bucket Oval bucket Microplate bucket (Set of 6) -
Page 17: Machine Description
2.6. Machine Description 1. Lid 2. Window for metrological speed measurement. 3. Control panel 4. Opening lid button 5. Power switch Fig. 1 ¾ Front View 6. Wheels (x4) 7. Ajustable feet (x4) Fig. 2 Vue de face 2.7. Interface description Touch-sensitive control-display. -
Page 18: Label
2.8. Label 2.9. Site preparation Doorway: 810 mm The location of the centrifuge must be able to resist 1020 twice the machine's weight in use (approximately 480 kg) i.e. 960 kg and be able to operate without vibrating. Vibrations diminish results centrifugation. -
Page 19: Electrical & Environmental Requirements
It is recommended that no less than two people are present to manually lift the centrifuge onto the laboratory bench. 2.10. Electrical & Environmental Requirements The KATRINA centrifuge must be connected directly to the switchboard, in 230V 50Hz single-phase or in a cable outlet junction box. - Page 20 Emissions: EN61326 Immunity: EN61326 Class A equipment is intended for use in an industrial environment. In the documentation for the user, a statement shall be included drawing attention to the fact that there may be potential difficulties in ensuring WARNING electromagnetic compatibility in other environments, due to conducted as well as radiated disturbances.
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Page 21: Operating The Katrina Centrifuge - Afi-C600R
3. Operating the KATRINA centrifuge - AFI-C600R 3.1. Levelling Mandatory operation : CAUTION Before using floor model centrifuge, adjust the level setting pads, following the procedure supplied onto the package box (Document Reference AP6.001 Unpacking & installation). Use supplied pads. -
Page 22: Rotor Assembly
3.3. Rotor assembly 2 people are necessary to handle the rotor. Clean and lubricate the motor shaft by depositing a thin film of the food grade grease supplied. Place the rotor above the drive shaft. Align the motor shaft wedge with the shape on the rotor. Slide the rotor on the drive shaft. -
Page 23: Opening The Lid
3.6. Opening the lid On the first start, the lid may not open right away due to a long storage in a cold environment. In this case, warm-up the hinges springs by repeated manual shifting of the lid downwards. To open the lid, press the button on the right side. 3.7. -
Page 24: Quick Start-Up
3.8. Quick start-up Before using the centrifuge, make sure it has been properly installed. Originale Notice - Rev.1.2 User Manual – Katrina p.22/ 55... -
Page 25: Operating
4.3. Access Administrator & Service Profile Pictogram Select Profile Enter Password Default Access Code : Administrator Profile : "0" Administrator Profile : Reserved to technician approved by AFI Centrifuge Validate Originale Notice - Rev.1.2 User Manual – Katrina p.23/ 55... -
Page 26: Modify Profile Code
4.4. Modify Profile Code Administrator & Maintenance Technician Profiles request access code. These codes can be modified. Picture Description Dial default access code : Press Renew code button. Dial Current Code & Validate Dial New Code & Validate Confirm New Code & Validate The New Code is saved ! 4.5. -
Page 27: Modify A Parameter
4.6. Modify a parameter Visual Instruction Control Select the program to edit Note: 99 programs are available, from 01 to 99. Edit the title screen by pressing on the title. Suppress the program name : Press "Del" for Entire program name or press "Delete"... -
Page 28: Set Speed / Centrifugal Force
4.7. Set Speed / Centrifugal force Select speed settings Dial new speed / centrifugal force. Save modifications Select program number. Validate 4.8. Convert rpm / cgf force Select speed settings Press Convert button to switch to another unit : Centrifugal force in rcf (Relative Centrifugal Force) Speed in rpm (Rotation Per Minute) The value is automatically converted. -
Page 29: Set Time Cycle
4.9. Set Time cycle Select time settings Enter time cycle, in hours : minutes : seconds Save modifications Select program number. Validate Note : In case of Time cycle setting, the cgf is set to "0". Time is master parameter, and cgf becomes the slave parameter. -
Page 30: Convert Temperature
4.12. Convert Temperature Select temperature settings Press Convert button to switch to another unit : Celsius degree (°C) Fahrenheit (°F) The value is automatically converted. Save modifications Select program number. Validate 4.13. Set Acceleration Slope Select acceleration settings Enter Acceleration value. Save modifications 9 : represents the fastest acceleration duration 0 : represents the longest acceleration duration. -
Page 31: Set Deceleration Slope
4.14. Set Deceleration Slope Select deceleration settings Enter deceleration value. Save modifications 9 : represents the fastest braking duration 0 : represents the longest braking duration : "free wheel" type. Select program number. Validate 4.15. Use a program to centrifuge Load buckets following instructions in §... -
Page 32: Open After An Electric Power Failure / Emergency Stop
While rotating, press onto STOP button The cycle is interrupted. The rotor slows down following deceleration slope 4.17. Open after an electric power failure / Emergency Stop This operation must be performed by a person trained in centrifugation-related risks. In case of power failure, the lid cannot be opened by the CAUTION engine unlocking control. -
Page 33: Precooling
4.18. Precooling A "Pre-cooling" cycle prior to centrifugation allows to cool the centrifuge down to 4 degrees Celsius so it is ready for temperature sensitive samples. Visual Instruction Control Access to the list of programs by pressing program number: Select the pre-cooling program: Rotor: Standard Precool This program is set to cool the centrifuge and accessories down to 4°C during a 22 minute cycle at... -
Page 34: Preferences Menu
Select the pre-warming program: This program is set to warm the centrifuge up to 20°C during a 20 min cycle at 5,100 RPM. Acceleration and Deceleration slope are both set on 9 and cannot be modified. Start the cycle by pressing The temperature inside the bowl will be shown on the display 4.20. -
Page 35: Speed Conversion
• Automatic lid opening • Sleep mode • Post Cool • Pre-Cool Function • Temperature Display Mode Visual Instruction Control Select the unit you want displayed by default: • rpm: Rotation Per Minute • rcf: Relative Centrifugal Force, or G Force Confirm Do not turn the centrifuge off at this point. -
Page 36: Access To Logbook
Visual Instruction Control Select how many cycles you want between each lubrication reminder. • No reminder (Not recommended) • 100 cycles (Default value) • 200 cycles • 300 cycles Confirm Visual Instruction Control The counters keep track of the life of the centrifuge. •... -
Page 37: Temperature Mode
Visual Instruction Control Select you unit you want displayed by default: • °C: Degree Celsius • °F: Degree Fahrenheit Confirm Visual Instruction Control Select the type of timer: • ON: The timer only starts when the centrifuge has reached the set speed in the program •... -
Page 38: Keyboard Type
This function is used to set keyboard type. Select keyboard type : • Qwerty. • Azerty: This is the default setting. Upper case letters Lower case letters Access numbers & special characters. Visual Instruction Color This function activates a beep when the cycle is complete. -
Page 39: Error Message Alarm
• OFF: No signal at the end of the run. This is the default. Visual Instruction Color This function activates a beep when an error message appears • ON : Upon error the signal is triggered Pressing the key stops the signal. •... - Page 40 This function maintains the temperature in the bowl between runs. • ON: After closing the lid the refrigeration, the unit will maintain the temperature for 4 hours. Note: Upon opening the lid the 4 hour timer will restart. • OFF: Bowl temperature is not maintained between runs.
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Page 41: Pre Cool Function
Allows Pre Cool to 4⁰C in air or rotor mode. • bowl. AIR: Cooling to the temperature in the This is the default. • ROTOR: Cooling for 22 minutes. At this point the rotor and accessories are cooled. Allow for display of air or rotor temperature. •... -
Page 42: Service Menu
4.21. Service Menu Visual Instruction Control This menu is only accessible to certified service companies. Exit Service access code is required. Only certified technicians are allowed to go further. 4.22. Information Visual Instruction Control Access the menu through this icon : This menu provides general information on the Software and Hardware of the centrifuge : •... -
Page 43: Care And Maintenance
5. Care and Maintenance All maintenance actions on this equipment must be performed by a qualified technician who is familiar with the proper maintenance CAUTION procedures equired for this equipment. The device and its accessories may be contaminated. Apply decontamination measures required before any maintenance. CAUTION Turn off the centrifuge power switch. -
Page 44: Rotor & Accessories
Use clear or distilled water to clean the rotor and buckets. A soft non-metallic brush or lint-free cloth can be used. Dry the rotor and accessories ensuring the least accessible areas are wiped. Image Operation To ensure a proper swinging movement of the buckets, clean all trunions of the rotor. -
Page 45: Dismantling The Rotor
5.2. Dismantling the rotor 2 people are required to handle the rotor. Image Operation 1. Open the lid. 2. Remove the rotor lid. Remove the buckets from swing-out rotor. Grab the wrench and place it onto the rotor nut. Turn the wrench counterclockwise while holding the rotor firmly. -
Page 46: Checking And Testing Accessories
Check that the grounding of the device is always properly secured by its power cable and the wall outlet it is connected to. The operator is not authorized to access the internal part of the equipment. An AFI Centrifuge authorized technician will ensure maintenance. -
Page 47: Error Messages, Troubleshooting, Option-Diagnostics
5.7. Error Messages, Troubleshooting, Option-Diagnostics Visual Instruction In case of a problem, the display shows an error message and an error number. Error / Message Cause Solution LOCK FAIL The lid is open when the centrifuge starts. Close the lid. Wait for complete closing of the lid before starting a new cycle. - Page 48 contact is not good. Contact a Certified Technician. LOCK CONTACTOR The power contactor doesn’t work. The micro contact was seen as open NOT CLOSED The open position switch was released after when it should have been seen as closed. the lid closed. Contact a Certified Technician.
- Page 49 Originale Notice - Rev.1.2 User Manual – Katrina p.47/ 55...
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Page 50: Wearing Parts & Consummable
5.9. Back to manufacturer In case of a need to return to AFI Centrifuge manufacturer of the device and / or its accessories, all elements must be decontaminated before transport, in order to protect the various parties involved and the environment. -
Page 51: Basics
6. Theoretical Basics of Centrifugation 6.1. Basics Relative Centrifugal Force In customary practices and usages of centrifugation, the quantization unit of angular velocity (denoted N), is the revolution per minute (abbreviated rpm, RPM, r / min, or r • min-1). This is a measure of frequency of rotation. -
Page 52: Nomogram
6.2. Nomogram RPM (r/min) RCF (Number of grams) Radius (mm) The nomogram graphically connects by a line segment a speed to the relative centrifugal force (RCF) as a function of the radius of rotation selected. In the example above, the speed of 2500 rpm corresponds to a relative centrifugal force of 550 g for a radius of rotation of 79 mm Originale Notice - Rev.1.2... -
Page 53: Logbook
6.3. Logbook Example of table to monitor the use of the centrifuge and accessories. Date Operator Duration T° Rotor Incidents Originale Notice - Rev.1.2 User Manual – Katrina p.51/ 55... -
Page 54: Return Coupon
Site Address Post code Town Country Phone Email Address Billing address (If different from the site Town address) AFI Centrifuge Contact information Society AFI CENTRIFUGE Address : ZI Nord Bazouges Post code 53200 Town Château-Gontier Description of your equipment Designation Ref. -
Page 55: Annex 1: Accessory Specifications
20 years Maximum Radius : 278 mm Microplate buckets Part number: AFI-BXDW6 Microplate bucket for Description : rotor AFI-RX6-1000 (Set of 6) Maximum speed 4300 rpm Maximum RCF 5519 xg Capacity standard Microplates 8 plates per bucket Capacity Deep well blocks 2 per bucket Originale Notice - Rev.1.2... - Page 56 266 mm Included : Stainless Steel extractor (Set of 6) Part number : AFI-MBL-1000 Description : Airtight Microplate lid for BXDW6 bucket (Set of 6) (*) The radius is the distance between the rotor shaft and the bottom of the inserts when the bucket / microplate...
- Page 57 Originale Notice - Rev.1.2 User Manual – Katrina p.55/ 55...
- Page 58 3, rue Nicolas Copernic ZA Nord Bazouges 53200 CHATEAU-GONTIER FRANCE Tél : +33(0)2 43 06 66 76 contact@ afigroups.com www.afigroups.com Non-contractual photos. All rights reserved, including photos and illustrations. Copyright ® AFI Centrifuge company – SIRET 513 982 645 00022.
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