Vitlab Micropipette Manual page 10

Accuracy (of one channel)
V – V
A [%] = · 100
0
V
0
Coefficient of variation (for one channel)
100 s
CV [%] =
V
Comparison actual values - nominal values (per channel)
Error limits: see tables 7.2.1 and 7.2.2 (for multichannel pipettes see tables 7.3.1 and 7.3.2)
or definition of individual error limits.
Result
If the calculated values for A [%] and CV [%] are (for multichannel pipettes per channel) smaller than
or equal to the error limits, the pipette is in good working order.
If the calculated values are larger than the error limits:
►
Verify, if all above instructions have been carefully followed step by step
►
Observe notes from the „Troubleshooting" section in the operating manual
► Recalibrate the VITLAB micropipette according to the steps in the operating manual
If these measures are not successful, we recommend you to make use of the VITLAB calibration service.
Possible volume errors and the resulting measures:
Error
Volume too small
Multichannel pipettes:
Volume too large
Other influences
10
®
Possible causes
Pipette tip not properly
attached
Seal or piston damaged
V- / O-rings damaged
Pushed pipetting button too
far
Pipette is not correctly calibrated
Temperature adjustment of the
device, room and water temper-
ature was not completed
►
Enter value into the test record
►
Enter value into the test record
Measures
► Use a new pipette tip and attach
it tightly
► Clean or replace seal resp. piston
(see operating manual)
►
Replace V- / O-rings
► Be very vigilant to not press be-
yond the first stop
► Recalibrate pipette
► Carry out temperature adjust-
ment