3D Histech Pannoramic DESK User Manual page 53

Pannoramic DESK 1.18.1 User's Guide
Live
To display a live image of the current specimen in the Live image display field, click
sample. This option has no effect on a running digitization process where no live image is
displayed. To display a live image during scanning, select Show images during scanning in
the Service tab.
Focus
To define focus limits, select Limit focus range. Set the upper and lower limits by selecting
the desired value in the selection boxes. This is useful if Auto Focus does not find the focus
on the specimen, but on the coverslip. Also, this makes the focusing sequence faster.
By using Set Current, the Lower limit value is set to -32 of the current focus value and the
Upper limit value is set to +32 of the current focus value.
Use the two arrow buttons to focus manually. This is useful when you are interested in an
area that remained blurry with Auto Focus (for example, the specimen is thicker in that
area). The manual focus is mainly for setup purposes, not for using the product as a
traditional microscope.
Define the manual focus step size by adjusting the Step size slider.
Search for the sharpest focus on the current field of view automatically by clicking
Multilayer mode
Select Single layer option if you want to scan a single layer of the slide.
By choosing the Extended focus option, the software selects the sharpest image from each
focus level for each image field, and combines them into one single image. Therefore those
parts that were blurry in auto/manual focus will also be sharp. This method guarantees the
maximum depth of sharpness, however, it reduces digitization speed.
With the Z-stack method separate layers are created and stored, and can be opened with
Pannoramic Viewer. The Z-stack slide contains the extended focus layer. Both for Extended
focus and Z-Stack scanning methods, you can determine the desired number of focus levels
with the Focus levels selection box. The current focus value is the starting point. If you set 5
for example, one current, two focus levels above and below FOVs are captured. Therefore, it
is advised to choose an odd number for the focus levels.
Focus step size determines the spacing between focus levels. One step is around 0.2 µm.
Default settings are: Number of focus levels: 7; Focus step size: 5.
May 29, 2014 - Rev. 1
. It will work only if you select an area in the preview image with the Position
.
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4 Software Description
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