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User Manual CELLINK
A B
Arvid
W allgrens
B acke
2 0
Gothenburg,
S E
4 13
4 6
SWEDEN
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“You cannot create new science unless you realize where the old science leaves off and new science begins, and science- fiction forces us to confront this.” Michio Kaku 2
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Table of Contents INTRODUCTION
O F
T HE
I NKREDIBLE
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5 SPECIFICATIONS
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6 HOW
D OES
I T
W ORK?
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7 ...
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INTRODUCTION OF YOUR INKREDIBLE 3D BIOPRINTER 4
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INTRODUCTION OF THE INKREDIBLE Hello and welcome to the exciting world of 3D Bioprinting. The technology you have in front of you has the potential of creating real, living human life and it is only your imagination that limits what you can do.
HOW DOES IT WORK? The INKREDIBLE bioprinter is an extrusion based 3D Bioprinter with dual extruder heads for bioprinting of human tissue models and organs. It is a cost-effective unit to enter the 3D bioprinting field and for innovators and beginners to start bioprinting living tissues at ease.
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UNPACKING YOUR INKREDIBLE 3D BIOPRINTER When unpacking your INKREDIBLE 3D Bioprinter, be careful not to grab onto any movable parts or lose components. We recommend you grab it by the top window frame and pull it out from the box. ...
[1x] SD card pre-loaded with sample objects to print [1x] USB Cable [1x] Portable oil-free air compressor with tubing [1x] STARTINK-Kit (1 prefilled Syringe with 3mL of CELLINK + 1 CELLMIXER + 10mL of Crosslinking solution) [3x] Empty cartridges [1x] 24 well plate [1x] Petri-dish Note: Unpacking should be done outside the laboratory environment.
SAFETY INSTRUCTIONS MALOPERATION OF THE INKREDIBLE 3D BIOPRINTER INSTRUMENT CAN LEAD TO SEVERE AND IRREVERSIBLE INJURIES IF THE SECURITY INSTRUCTIONS ARE NOT FOLLOWED! NOTE: In order to respond to the INKREDIBLE 3D Bioprinter and its relevant accessories security requirements, the instrument has to be operated by trained personnel, which is aware of the risks and dangers that can be induced to themselves, as well as for the staff being in close relation with the INKREDIBLE 3D Bioprinter.
NOTE: Make sure the supplied air pressure does not exceed the admissible range of 4 bars (400 kPa). PERSONNEL PROTECTIVE EQUIPMENT Always wear protective glasses and secure the environments of the instrument to other personnel while operating the INKREDIBLE. All laser safety equipment, including laser safety glasses, must be adapted to the installed UV curing kit.
OPERATING THE INKREDIBLE Only trained staff is allowed to operate the instrument being aware of the risks and potential injuries (non reversible damages can be caused). NOTE: OPENING OF THE INSTRUMENTS ELECTRICAL PANEL WILL AUTOMATICALLY REVOKE THE WARRANTY. The manufacturer certifies that the INKREDIBLE is conforming to requirements. The manufacturer gives an 8 months warranty on the moving parts in the INKREDIBLE bioprinter.
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7. The INKREDIBLE 3D bioprinter can be switched on with the on/off button [1]. 8. Connect the air tube (provided) to the air compressor and then to the back of the Bioprinter. Turn on the air compressor and regulate the air pressure to 400 KPa. Note: Air compressor should be connected to a separate socket (not in the same fuse) to avoid any electromagnetic interference with the INKREDIBLE Bioprinter.
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-Turn the Control Knob Wheel [5] until the Ph 1 needle is right above the petri dish or well plate well. A little space of about 0.1mm should be visible between the print bed and the needle tip. You can use a piece of paper to slide between the needle and the petri dish to measure the distance.
(Printing pressure and printing speed). Configurations, pressure adjustments, and any development of printable biomaterial is upon the customer`s responsibility. CELLINK is not responsible for any clogging or damages caused by any user dependent biomaterials bioprinted with the INKREDIBLE.
BIOPRINTING WITH CELLINK SOFTWARE (REPETIER HOST) INTRODUCTION TO G-CODE PROGRAMMING The special M codes for INKREDIBLE 3D Bioprinter M712 to M732 are used to set the offset between the printheads printhead 1 and 2 are the syringes and printhead 3 is the led.
CLEANING AND MAINTENANCE The INKREDIBLE 3D Bioprinter is coated with a chemical resistant coating. It can be sprayed with chlorine or ethanol in order to sterilize the surface. It is advised to lubricate the axis once in a month to ensure a good functioning of the 3D bioprinter.
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Once a return is received (for example a returned bioprinter), CELLINK will then send back a new unit. CELLINK will pay to send out the bioprinter and parts to correct a problem, but will not be responsible for return costs. Proper packaging is required in the rare case of a returned product or part, and the product itself must arrive back in the condition in which it was originally received.
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There is no warranty issued when the INKREDIBLE is used with exotic printing material not personally recommended by us at CELLINK. Foreign materials can damage the system. The warranty also does not cover operating the bioprinter at not recommended speeds and settings.
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This guarantee and limited warranty are sole and final as they stand in reference to INKREDIBLE, CELLINK and it’s products where permitted by law. Any implied warranty or fitness is limited to this 3 months (90 day) warranty starting from the date of purchase.