Singer Instruments ROTOR HDA User Manual
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Singer Instruments ROTOR HDA User Manual

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User GUide v1.2

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Summary of Contents for Singer Instruments ROTOR HDA

  • Page 1 User GUide v1.2...

  • Page 2: Before You Start

    · Ensure to keep transit hardware in a safe place. These will be required should the rOTOr HdA require moving. · During Pad Head removal, always ensure the push cylinder is cleared before moving away from the carriage. The push cylinder runs from the carriage arm into The stinger/Pad Head.
  • Page 3 Follow these instructions alongside the on-screen instructions to get the most out of the rOTOr HdA, High Throughput screening robot. This guide outlines basic operation of the rOTOr HdA as well useful maintenance advice. read through this guide and you’ll be ready for...
  • Page 4 anatomy & features screen Handle Lid Lifters Fast Buttons Mci Touch screen Protective screen dump Zone carriage Arm rs232 shuttle input emergency stop Hopper Loading Bay dump drawer Black Bay Turntable Pad Head Hopper UV decontamination roll-over cover control Panel...
  • Page 5 & features removable Transit clamps Beer Bottle Opener removable Transit Bar Air Line connection NOTe: ensure to keep transit hardware in a safe place should the rOTOr HdA require moving. Mci Mounting Bracket Power switch Power in singerinstruments.com...
  • Page 6 removaBles BLAck BAy · The Black Bay is a loading bay for source and target plates. you will need to remove the Black Bay to load plates. · if you own The stinger single colony picker then you will need to remove the Black Bay in order to change between the Pad Head and The stinger.
  • Page 7 repaDs ™ 96 sHOrT Compatible media Compatible densities · · solid agar · · · 1536 96 LONG Compatible plates Compatible densities · · Liquid · · solid agar · · 1536 384 sHOrT Compatible plates Compatible densities · · solid agar ·...

  • Page 8: Mechanical Overview

    mecHanical overview · · Source Plates or Target Plates are loaded into the Black Target Plates or Source Plates are loaded into the Turntable. · Bay. source plates are the plates that already have the if loading four plates, the front two plates are loaded first. desired strains on.
  • Page 9 mecHanical overview · · The Pad Head moves to a Target Plate and deposits the The Pad Head moves to the Dump Zone and drops the sample of cells. used RePad™. · · Used RePads™ are collected in the Dump Drawer ready to be Follow the simple step-by-step instructions for loading/ disposed of.
  • Page 10 · Open the Roll-over Cover. · insert the Power Cables into the ROTOR HDA and switch on the mains power. · Turn on the ROTOR HDA Power Switch. · Follow the simple on-screen instructions to work through the ROTOR HDA initialisation procedure.
  • Page 11 · clear all objects from the ROTOR Stage, so nothing will obstruct the initialisation routine. · select OK On-screen or use the Fast Buttons. · The ROTOR HDA will perform a start-up routine. singerinstruments.com...

  • Page 12: Software Overview

    The source could be colonies grown on solid agar, or cultures grown in liquid media. in solid agar, the rOTOr HdA currently supports colony density up to 24576. in liquid media, the rOTOr HdA supports multi-well plates at 96 and 384-density.
  • Page 13 software overview · select your RePad™. The types of rePads™ available are only those compatible with your previous selection. · in this example we select Short Pin Pad 96-Density. · NOTe: short pin pads are used to pin colonies from solid agar to solid agar.
  • Page 14 program overview NOTe: running a program is easy. Just follow the on-screen instructions! · This is the Program Preparation Page. Here you can fine-tune the program using the options on the left. A detailed overview of these options can be found on pages 18-21.

  • Page 15: Program Overview

    · Follow the instructions and load the appropriate RePads™ into the Pad Hopper. · The ROTOR HDA will now count the number of Pin Pads loaded to ensure there are enough to complete the program. · The ROTOR HDA will now start pinning the colonies for replication.
  • Page 16 program completion · if you wish to generate more than 4 replicates of a target plate, you can repeat the program with one click. · Make your selection. · when pinning is completed, you will be instructed to remove the Plates. ·...

  • Page 17: Loading Plates

    Home Screen. Screen. Here you can access the advanced options and online support. · · Rotate: This rotates the Turntable. Off: Press to turn off the ROTOR HDA. · Reset: This resets the ROTOR HDA. LOAdiNG PLATes · Load Plate ·...
  • Page 18 software icon guiDe AdVANce OPTiONs IcOn nAME SETTIng DEScrIPTIOn UnIT DEFAUlT recycle rePads™/pins are always dumped. General>recycle recycle Full rePads™/pins are recycled for the General>recycle duration of the program. recycle Until rePads™/pins are recycled for the General>recycle repeat duration of one cycle of the program. recycle during rePads™/pins are recycled for the...
  • Page 19 software icon guiDe IcOn nAME SETTIng DEScrIPTIOn UnIT DEFAUlT Offset Manual select a manual offset before each source>Offset Boolean source pinning. Offset Fixed A pre-specified fixed offset is used for source>Offset Boolean selected source pins. source The pressure that the Pad Head will use source>Pinning Varies for Pinning...
  • Page 20 software icon guiDe IcOn nAME SETTIng DEScrIPTIOn UnIT DEFAUlT wet Mix On/Off Liquid mixing can be used to invigorate source>wet Mix Boolean source the cells in a liquid solution. Liquid mixing uses either a circular or helical movement. source The diameter of the mix applied to both source>wet Mix Mixing the x and y axis.
  • Page 21 software icon guiDe IcOn nAME SETTIng DEScrIPTIOn UnIT DEFAUlT repeat Liquid The number of times each target pin- Target >Pinning integer Target ning will repeat. Pinning dry Mix On/Off Agar mixing can be used to ensure that Target >dry Mix Boolean Target a good contact with the target media is...
  • Page 22 GUi. · Pad dispenser rim fingers ensure accurate and repeatable NOTe: For servicing, the rOTOr HdA will require rePad™ positioning. reasonable free space all round. TUrnTAblE · WEIgHT diameter: 360mm ·...

  • Page 23: Graphical Interface

    · it and will run only intermittently. The pipe connecting short 6411: 20 per sleeve/1000 per box the compressor to the rOTOr HdA is 6mm dia (1/4”). The running costs compressor may be sited away from the rOTOr HdA ·...
  • Page 24 pinning examples 1:4 Array 1:4 ArrAY · 4x 96-density plates are combined onto 1x 384-density plate. 1:4 single source 1:4 SInglE SOUrcE · each colony from a 1x 96-density plate replicated in quadruplicate to a 1x 384- density plate. These protocols can be applied at all pinning densities.
  • Page 25 · · select a Delicious Beer. Pick a strong one - it’s been a Locate the Bottle Opener on your ROTOR HDA. hard day! · · insert the Beer Bottle into the Bottle Opener. Lever the Beer Bottle to remove the Bottle Cap ·...
  • Page 26 notes...
  • Page 27 notes singerinstruments.com...
  • Page 28 notes...
  • Page 29 notes singerinstruments.com...
  • Page 30 roadwater, watchet, somerset. TA23 0re +44 (0)1984 640226 (tel) +44 (0)1984 641166 (fax) contact@singerinstruments.com singerinstruments.com scan to visit weBsite for more Helpful tips anD tutorials!

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