Related Manuals for Varian ProStar 701
Summary of Contents for Varian ProStar 701
- Page 1 Varian, Inc. 2700 Mitchell Drive Walnut Creek, CA 94598-1675/USA ProStar 701 Fraction Collector Control Galaxie Driver Manual ©Varian, Inc. 2005 03-914947-27:Rev.2...
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Page 2: Table Of Contents
Configuring the Varian ProStar 701 Fraction Collector..........4 Configuring the Synopsis ....................5 Building a Method ................... 7 Creating a New Method.......................7 Control Method Section for the Varian ProStar 701 Fraction Collector......10 Collector Parameters Control..................12 Fraction Collector Mode Control .................14 Rack Control .......................17 Collection Chain Control .....................19... -
Page 3: Introduction
General This document describes the configuration of the Varian ProStar 701 Fraction Collector driver. It also explains how to build a control method for this driver, how to start and view a running acquisition and what actions are available during an analysis. -
Page 4: Installation And Configuration
To install the Galaxie Drivers software refer to the Galaxie Data System Installation Guide. Configuring the Communication Server To communicate with the ProStar 701 module an RS232_Interface or an RS232_PC communication bus must be configured on the acquisition server. The parameters of the bus to must be the following:... -
Page 5: Configuring The Driver
The driver software configuration is done in the Galaxie Configuration Manager when a new chromatographic system is created. Configuring the Varian ProStar 701 Fraction Collector Click on the button corresponding to the Varian ProStar 701 Fraction Collector driver and the configuration screen appears. -
Page 6: Configuring The Synopsis
Define the number of ProStar 701 Fraction Collector installed in the Number of instrument spin edit. Up to four ProStar 701 Fraction Collectors can be defined. For each fraction collector, define the address in the Fraction Collector X ID field. Then press the button, to connect to the instrument. - Page 7 (temperature, flow, signal, etc.) depending on the installed devices. For example, the following pictures represent the synopsis of the Varian ProStar 701 Fraction Collector: Galaxie Configuration Manager Screen Galaxie Chromatography Data System Screen NOTE: Once the system has been created and correctly configured, it must be associated with one or more projects in order to start some acquisitions.
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Page 8: Building A Method
1. To create a new method, select the FILE / NEW METHOD option from the Galaxie Chromatography Data System main menu. A wizard guide will appear and provide assistance during the first steps of method creation: ProStar 701 Fraction Collector Control Manual - 7 - 03-914947-27:2... - Page 9 3. Once this field is completed, click on the Next button to move to the second step of the method creation: ProStar 701 Fraction Collector Control Manual - 8 - 03-914947-27:2...
- Page 10 The instrument control section must be defined in order to start an acquisition. In the following part of this guide, only the control section for the Varian ProStar 701 Fraction Collector is detailed. Other parameters such as Injection Volume, Vial and Rack number may be necessary and are defined in the acquisition section.
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Page 11: Control Method Section For The Varian Prostar 701 Fraction Collector
Varian ProStar 701 Fraction Collector Control Method Once the new method is created, select the control section in the lower part of the browser. The following screen appears: Press the button to display the System Control Method Advanced Tools window: to send the current method to the instrument. - Page 12 Click on the button to display the ProStar 701 Fraction Collector control method section. It contains four sub-sections: Collector Parameters, Fraction Collector Mode, Racks, and Collection Chain. Click on the required sub-section to define the corresponding parameters that will be used during the chromatographic run.
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Page 13: Collector Parameters Control
Note that some parameters of the other sub-sections will be activated or may change according to the entry. Select in the Pattern list weather the fraction collector uses the serpentine or the unidirectional mode to collect samples. ProStar 701 Fraction Collector Control Manual - 12 - 03-914947-27:2... - Page 14 This allows the use of collection options that cannot be sent to the fraction collector. If this is selected, the collection option in the fraction collector will be used. ProStar 701 Fraction Collector Control Manual - 13 - 03-914947-27:2...
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Page 15: Fraction Collector Mode Control
The peak separation is based on the range in the slope of the baseline over a wide range of user defined peak widths. If selecting this ProStar 701 Fraction Collector Control Manual - 14 - 03-914947-27:2... - Page 16 Detector Signal: it is the detector output value. Three choices are available: 10mV, 100mV and 1 V. Threshold level: it is the percentage (0 to 100%) of the full scale height since which peaks are collected. ProStar 701 Fraction Collector Control Manual - 15 - 03-914947-27:2...
- Page 17 Up to 25 lines can be defined. Use the following buttons to edit the table: to add a line to the table. to delete a line in the table. to clear the entire table. ProStar 701 Fraction Collector Control Manual - 16 - 03-914947-27:2...
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Page 18: Rack Control
(depending on whether Time or Drop mode is defined in the Collector Parameters screen) that can be contained in a tube. If this value is exceeded, the fraction collector moves to ProStar 701 Fraction Collector Control Manual - 17 - 03-914947-27:2... - Page 19 Beaker Use of a rectangular rack with max 16 rows and 16 tubes per row. User has to define the first and last tube position, ProStar 701 Custom Fraction Collector will calculate the row and column spacing. The configuration is done from the ProStar 701 Fraction Collector instrument.
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Page 20: Collection Chain Control
To use the Collection Chain option, it is ProStar 701 Fraction Collector Control Manual - 19 - 03-914947-27:2... - Page 21 Sense Peak by Time option in the Fraction Collector Mode sub-section. Note that the overflowing is not managed by the ProStar 701 Fraction Collector in the Overlay case which means the Fraction Size parameter (Racks sub-section) is not taken into account.
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Page 22: Starting And Viewing An Acquisition
In the Quick Start window, select the system, the method and optionally the project if connected in "All project" mode. 2. Press the OK button. The following screen is displayed: ProStar 701 Fraction Collector Control Manual - 21 - 03-914947-27:2... - Page 23 In a multiple injector system, the same parameters entered for the first injector must be entered for the other injectors before starting the acquisition. ProStar 701 Fraction Collector Control Manual - 22 - 03-914947-27:2...
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Page 24: Sequence
3. Start the sequence by clicking on the button. NOTE: For more details about how to start an acquisition, refer to the Galaxie Chromatography Data System User’s Guide. ProStar 701 Fraction Collector Control Manual - 23 - 03-914947-27:2... -
Page 25: Running Acquisition Status
A window is activated which is composed of two parts: the running chromatogram and the status window where the driver modules are listed. Varian ProStar 701 Fraction Collector Status Click on the button corresponding to the ProStar 701 Fraction Collector driver and the fraction collector status screen will appear. - Page 26 ProStar 701 Fraction Collector Control Manual - 25 - 03-914947-27:2...
- Page 27 The user has to select the program stored in the ProStar 701 Fraction Collector driver to start. when pressing this button a message asks to confirm the action and the button is transformed into . This forces the ProStar 701 Fraction Collector to send the solution in the Drain (waste) or to collect it in tubes.
- Page 28 Note that when performing collection under Galaxie control, the program A of the ProStar 701 Fraction Collector is erased and replaced by the one defined in the Galaxie method. Overview Window...
- Page 29 The buttons available in the right part of the Overview part have the same functions as in the Status window. Not Ready: no information is listed. Events: contains the communication state between Galaxie and the ProStar 701 Fraction Collector. ProStar 701 Fraction Collector Control Manual - 28 - 03-914947-27:2...