Related Manuals for Varian 210-MS
Summary of Contents for Varian 210-MS
- Page 1 Varian, Inc. 2700 Mitchell Drive Walnut Creek, CA 94598-1675/USA 210-MS and 220-MS GC/MS Ion Trap Mass Spectrometer Installation Procedure 03-954143-55:Rev. 1 1 of 33...
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Page 2: Table Of Contents
Contents Overview............................4 Typical Installations .........................4 Setting Up..........................5 Checking the Installation Site .............................5 Unpacking...................................5 Connecting Components ............................5 Setting Up the GC........................8 Filling the Cal Gas Vial ...............................8 Plumbing and Purging ..............................8 Installing the Column in the GC..........................9 Installing the Column in the MS..........................9 Setting Up the Foreline Pump ....................9 DS-42 Pump ................................9 Powering Up the MS .......................11... - Page 3 Acquiring BZP................................27 Calculating the Signal-to-Noise Ratio........................28 Familiarizing the User Representative..................29 Creating a Sample List .............................29 Activating and Running a Sample List........................29 Reviewing Results ..............................30 Basic Source Maintenance............................30 Checklist for Familiarizing the User Representative....................31 Appendix 2: Spare Parts ......................32 Kits, Assemblies, Boards, and Cables .......................32 Trap Components ..............................32 Pumps ................................33 03-954143-55:Rev.
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Page 4: Overview
(helium), and CI gas purity (methane). Use these procedures to set up the 210-MS and the 220-MS GC/MS Ion Trap (210/220-MS GC/MS), meet product specifications, and familiarize the customer with the system. -
Page 5: Setting Up
Report all shipping damage or missing parts to the Customer Service department (Phone: 925-939-2400, Fax: 925-945-2360, email: orders.csb@varianinc.com), Varian, 2700 Mitchell Drive, Walnut Creek, CA, 94598. Note any shipping damage or missing items on the installation checklist and in the Installation Tracking Database. - Page 6 4. Remove the turbo pump shipping restraint from the MS. 5. Remove the shipping restraints from the GC including the metal dowel at the rear of the GC. 6. Instruct the customer to keep the removed restraints, in case the system needs to be transported.
- Page 7 J401 Analog Out J401 connection on top of 450-GC J43 Remote Option J43 Remote Option on back of 210/220-GC/MS 10. Connect the USB cable to the rear of the MS at J42 USB and to a USB port on the PC. 11.
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Page 8: Setting Up The Gc
Setting Up the GC Filling the Cal Gas Vial 1. Remove the empty Cal Gas vial from behind the MS front door by loosening the two mounting screws and twisting the vial while pulling down. Do not overfill the Cal Gas vial installed in the MS. Overfilling can result in FC-43 contamination that interferes with Cal Gas adjustments. -
Page 9: Installing The Column In The Gc
Installing the Column in the GC NOTE: the only injector for the 431-GC is the 1177. The 450-GC has the injector the customer ordered. 1. Install the column that shipped with the instrument to achieve the best results for the installation tests. If an 1177 injector is installed on the GC, use the 1177 to pass the sensitivity specifications. - Page 10 Do not place the foreline pump on the bench with the MS. Vibrations from the foreline pumps will degrade the MS performance. 4. Place the pump under the bench. Do not lengthen the vacuum hoses to prevent degrading the vacuum. The foreline pumps must be connected to a ventilation system.
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Page 11: Powering Up The Ms
Powering Up the MS 1. Turn on the power switch of the MS. 2. Turn on the CPU and monitor. 3. Open MS Workstation. 4. Allow the foreline pump and the turbo pump to run for about 30 minutes before assuming software control. This allows the pumps to become properly lubricated. -
Page 12: Preliminary Tuning
• Transfer Line: 230 °C 5. Click the Bakeout tab and enter these temperatures: • Trap: 220 °C, • Manifold: 120 °C • Transfer Line: 250 °C 6. Set a Hold Time that ends bakeout about 2 hours before you plan to arrive the next day. -
Page 13: Adjusting The Rf Ramp
Adjusting the RF Ramp 1. In the Adjustments tab in Manual Control, click Adjust RF Tuning. 2. Adjust the RF tuning rod to minimize the Highest value in the upper right corner of the Counts area. 03-954143-55:Rev. 1 13 of 33... - Page 14 VENT CAL GAS CI GAS RF ADJUSTMENT Click Save Results when finished. 03-954143-55:Rev. 1 14 of 33...
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Page 15: Adjusting Cal Gas
Adjusting Cal Gas To adjust the cal gas: 1. Open the MS door and ensure that the Cal Gas vial is full. 2. Click Adjust Cal Gas. 3. Adjust the Cal Gas needle valve behind the MS front door until the display shows that the Cal Gas pressure is OK. -
Page 16: Auto Tuning
Auto Tuning After performing the Adjust RF Tuning and Adjust Cal Gas, the instrument is ready to be Auto Tuned. Auto Tuning To auto tune EI and CI: 1. Click Auto Tune. 2. Click the Method tab and select the following checkboxes: •... -
Page 17: Testing Specifications
Testing Specifications Specification Table 210-MS Ionization Test Sample Amount RMS S/N Library Match Mode Measured Specification (any search type) PN 393065201 1 pg OFN ≥ 50:1 ≥ 500 (1 pg/µL OFN, 5 pg/µL BZP) 220-MS Ionization Test Sample Amount RMS S/N... -
Page 18: Acquiring Ofn
Acquiring OFN 1. Use the Inject Single Sample option. 2. Use a lower air gap to avoid losing sample through the tip of the needle. Ensure the syringe plunger is not forced out of the syringe barrel if using a Pressure Pulse. - Page 19 3. Click-and-drag a zoom highlight box around the OFN m/z 272 ion. 4. Right-click the apex scan of the 272 single ion chromatogram to display the Chromatogram menu. 03-954143-55:Rev. 1 19 of 33...
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Page 20: Calculating The Signal To Noise Ratio
Calculating the Signal to Noise Ratio 1. Click Chromatogram Plot Preferences. 2. Click the Chromatogram Plot tab and do the following: 3. In the Plot area, select Filtered Data Only 4. In the Filtering area, select Smooth Data, type 5 in the Point Smooth field, select Savitsky Golay for the Smoothing Method, enable Remove Spikes and type 4 for the Spike Threshold Factor. - Page 21 Right-click the apex scan of the 272 single ion chromatogram to display the Chromatogram menu. 7. Click Calculate S/N to generate the RMS signal-to-noise ratio. The following shows both the menu and the S/N data. 03-954143-55:Rev. 1 21 of 33...
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Page 22: Library Searching
Library Searching 1. From the Chromatogram menu, click Set Select Spectra to Average, and select 3. 2. From the Chromatogram menu, select Edit Background Correction. 3. Click Auto Background Correction. The Background Correction Spectra Count is displayed. 4. Click Done. 5. - Page 23 the spectrum was background corrected 3 Spectra Averaged Background Corrected 6. From the Search menu, select Library Search Active Spectrum. 7. From the Library List select Tutorial. 03-954143-55:Rev. 1 23 of 33...
- Page 24 8. Octafluoronaphthalene has the highest match score > 500. 9. Click the NIST Library Search button. 10. Select Spectrum Search to edit the library search parameters. Suggested library search parameters are displayed. 03-954143-55:Rev. 1 24 of 33...
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Page 25: Ci Methodology
CI Methodology The following explains the method to use to determine the signal to noise response for the 220-MS CI option. Installing Liquid CI 1. Condition the CI plumbing to remove air and water by opening the CI solenoids in Manual Control, before introducing methanol into the CI plumbing 2. -
Page 26: Adjusting Liquid Ci Reagent Flow
Adjusting Liquid CI Reagent Flow 1. Click the Adjustments tab in Manual Control. 2. Select the reagent Methanol, from the drop down list. 3. Click Adjust Cal Gas. NOTE: Minimize contamination by opening the CI needle valve only as needed. 4. -
Page 27: Methodology
Methodology NOTE: The system arrives with a CD containing factory test method and data files. Use these method files to do the sensitivity tests and retain the test files CD for your records. 1. Ensure the MS temperatures for this acquisition are set as follows: •... -
Page 28: Calculating The Signal-To-Noise Ratio
Calculating the Signal-to-Noise Ratio 1. Use the quick load option to load the BZP data file into MSDR. 2. Plot a single ion chromatogram of the m/z 183 ion. 3. Click the apex scan to display the spectrum. 4. Background correct the spectrum. 5. -
Page 29: Familiarizing The User Representative
Familiarizing the User Representative Familiarize the User Representative with the software and the basic operation and maintenance of the MS. Determine if the following topics were covered when the preceding tests were done. If not, or if the User Representative would like a review, then cover the indicated topics. -
Page 30: Reviewing Results
Reviewing Results 1. Click the Review/Process MS Data button. MS Data Review opens and shows the most recent data file. Select other data files from the tree on the left. Basic Source Maintenance Loss of sensitivity over time is usually due to a dirty or poorly optimized trap and contaminated trap components. -
Page 31: Checklist For Familiarizing The User Representative
Checklist for Familiarizing the User Representative Place a check in the checkbox to indicate that the topic was covered. Topic Performed □ Starting up and closing down the system, including MS instrument control. Controlling the vacuum system, starting and venting the pumps, monitoring □... -
Page 32: Appendix 2: Spare Parts
Appendix 2: Spare Parts Kits, Assemblies, Boards, and Cables Part Number Description 393000591 Assembly, Transfer Line (115V) 393000592 Assembly, Transfer Line (230V) 393033401 Cable, Transfer Line heater (115V) 393033402 Cable, Transfer Line heater (230V) 393000891 Assembly, Vacuum Manifold (115V) 393000892 Assembly, Vacuum Manifold (230V) 393074301 Assembly, Ion Gauge... -
Page 33: Pumps
Pumps Part Number Description 393077001 Mechanical Pump, 90 L/min, 115V Varian DS42 393077002 Mechanical Pump, 90 L/min, 230V Varian DS42 392035800 Screen, Turbo Pump (V-81) 392051800 7 ft Length Tygon® Tubing 393076401 Turbo Molecular Pump (V-81) 9499305 Inland 31 Vacuum Pump Oil...