Configuring the Driver......................4 Configuring the Varian ProStar 430 Autosampler............4 Configuring the Synopsis ....................9 Building a Method ................. 11 Creating a New Method.....................11 Varian ProStar 430 Autosampler Control Method .............13 Injection Control ......................15 Timed Events Control....................19 Automix Control......................21 User Program Control ....................24 Starting and Viewing an Acquisition............
The ProStar 430 is an autosampler that offers a wide variety of possibilities for HPLC. It incorporates among other things the following features: - suitable for use with various types of plates by various manufacturers.
To install the Galaxie Drivers software refer to the Galaxie installation Guide. Configuring the Communication Server To communicate with the ProStar 430 module an RS232_Interface or RS232_PC communication bus must be configured on the acquisition server. The parameters of the bus must be the following:...
Configuring the Driver The driver software configuration is done in the Galaxie Configuration Manager. Configuring the Varian ProStar 430 Autosampler Click on the button corresponding to the Varian ProStar 430 driver and the configuration screen appears. The toolbar enables to easily scroll through the configuration windows: to select the first configuration window.
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Communication Server Configuration program. In the Autosampler Device Identifier field, enter the device identifier of the ProStar 430 Autosampler. NOTE: For more details about the Bus configuration, refer to the Configuring the Communication Server section. ProStar 430 Autosampler Control Manual...
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Check Integrated stream switching if an optional high pressure 6-port valve is installed in the autosampler. Check Alarm buzzer, Key click and/or Error beep to switch sound signals on. ProStar 430 Autosampler Control Manual - 6 - 03-914947-33:2...
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Hardware parameter group: Tray type/Vial type Select the plates that are to be used on the Varian ProStar 430. Five choices are available: 96 low wells. 96 high wells. 384 low wells. 48 standard vials. 96 user-defined wells. NOTE: The 96 user-defined wells plate requires that the plate dimensions and settings be entered from the front panel of the Varian ProStar 430.
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Last transport vial (1 to 4) Enter the transport vial number of the last transport vial. The ProStar 430 scans the transport vial area starting with the first location selected and ending with this location. If no vial is found, an error will be reported.
4. Once the synopsis configuration is complete, it will be displayed in the Galaxie Chromatography Data System, in the Systems tab. In this tab, the Overview part will show the synopsis of the system with some parameters displayed ProStar 430 Autosampler Control Manual - 9 - 03-914947-33:2...
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(temperature, flow, signal, etc.) depending on the installed devices. For example, the following pictures represent the synopsis of the Varian ProStar 430: Galaxie Configuration Manager Screen Galaxie Chromatography Data System Screen NOTE: Once the system has been created and correctly configured, it must be associated with one or more projects in order to start some acquisitions.
1. To create a new method, select the FILE / NEW METHOD option from the Galaxie Chromatography Data System main menu. A wizard guide will appear and provide assistance during the first steps of method creation: ProStar 430 Autosampler Control Manual - 11 - 03-914947-33:2...
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The instrument control section must be defined in order to start an acquisition. In the following part of this guide, only the instrument control section for the Varian ProStar 430 is detailed. Other parameters such as Injection Volume, Vial and Rack number may be necessary and are defined in the acquisition section.
NOTE: For more details about the programming of each method section, turn to the Galaxie Chromatography Data System User’s Guide. Varian ProStar 430 Autosampler Control Method Once the new method is created, select the control section in the lower part of the browser.
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Click on the button to display the Varian ProStar 430 control method sections. It contains four sub-sections: Injection, Timed Events and Automix or User program. The User program sub-section appears when this option is selected in the Injection sub-section, Injection mode parameter.
Full loop, µl pick-up or User Program. If User program is checked, the Automix control sub-section is replaced by the User program sub-section. NOTE: Refer to the User Program sub-section for more explanation of this injection mode. ProStar 430 Autosampler Control Manual - 15 - 03-914947-33:2...
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If this option is not checked, the temperature between the injections will be kept. ProStar 430 Autosampler Control Manual - 16 - 03-914947-33:2...
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Check Use air segment to inject a sample with an air segment between the sample and the wash solvent. If Use air segment is checked, the ProStar 430 will draw an air segment of 5 µL before the flush volume prior to an injection. The air segment will not be injected into the analytical system.
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The Needle height is the distance between the needle point and the plate holder (default: 5 mm ; range: 0-40 mm). This value is only used in injection modes (this value is programmable in case of mix). ProStar 430 Autosampler Control Manual - 18 - 03-914947-33:2...
Check the Use timed events box to activate the program table. Use the following buttons to edit the table: to add a line to the table. to delete a line in the table. to clear the entire table. ProStar 430 Autosampler Control Manual - 19 - 03-914947-33:2...
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If the end time exceeds the programmed analysis time, this end time overrules the run time and the next injection will be started as soon as the timed events program has finished. ProStar 430 Autosampler Control Manual - 20 - 03-914947-33:2...
Automix Control Use Automix parameter group: Check the Use Automix box to activate the ProStar 430 mix capability: Select from the lists the Transport vial number that contains each Reagent fluids (A…D). Five choices are available: None, Transport 1, Transport 2, Transport 3 and Transport 4.
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Dispense to Sample, Waste, Destination or Reagent A…D a programmed volume (Amount) from the buffer tubing. It is possible to dispense a larger volume than the volume aspirated in previous actions. The aspirated Amount will be ProStar 430 Autosampler Control Manual - 22 - 03-914947-33:2...
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0 µL must be programmed at the desired position. Repeat the X entered last Step(s). The Count parameter defines how many times the Step(s) must be repeated. Rinse+Wash a programmed needle volume (Amount). Buffer is rinse to waste. ProStar 430 Autosampler Control Manual - 23 - 03-914947-33:2...
NOTE: When this mode of injection is selected, the Timed Events sub-section becomes inactive. The program of the auxiliaries, SSV and P3 output is done directly in the User Program sub-section. ProStar 430 Autosampler Control Manual - 24 - 03-914947-33:2...
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Amount which can be aspirated is the total volume of the syringe. The Speed of the syringe aspiration can be selected from 1 to 9, and the syringe Height from 0 to 40 mm. ProStar 430 Autosampler Control Manual - 25 - 03-914947-33:2...
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Solvent valve programs the positions (from 1 to 6) of the high pressure Solvent Selection Valve. Stream valve programs the positions (1-6 or 1-2) of the high pressure ISS valve. ProStar 430 Autosampler Control Manual - 26 - 03-914947-33:2...
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Inject position enables to start the data acquisition at this step of the program when Start run on inject marker is selected. Wait Input 1…4 programs a pause in which the ProStar 430 waits for one of the four inputs to become Low Level or High Level before continuing with the next step.
Quick Start (Single Acquisition) 1. Press the button. In the Quick Start window, select the system, the method and optionally the project if connected in "All project" mode. 2. Press the OK button. ProStar 430 Autosampler Control Manual - 28 - 03-914947-33:2...
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In a multiple injector system, the same parameters entered for the first injector must be entered for the other injectors before starting the acquisition. ProStar 430 Autosampler Control Manual - 29 - 03-914947-33:2...
3. Start the sequence by clicking on the button. NOTE: For more details about how to start an acquisition, refer to the Galaxie Chromatography Data System User’s Guide. ProStar 430 Autosampler Control Manual - 30 - 03-914947-33:2...
Varian ProStar 430 AutoSampler Status Click on the button corresponding to the ProStar 430 driver and the autosampler status screen will appear. It contains up to five sub-sections depending of the installed options: Status, Remote control, Plate cooling, Plate feeder and Overview.
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Green lights indicate if the corresponding relay is activated or not. Each relay can be opened or closed by clicking on the button. Options parameter group: Solvent switch indicates the solvent stream which is used at the specified time. ProStar 430 Autosampler Control Manual - 32 - 03-914947-33:2...
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The lock button is then active again. Tools parameter group: Click on the button to move the plate to the left. In this position, the plate can be replaced without damage to the equipment. ProStar 430 Autosampler Control Manual - 33 - 03-914947-33:2...
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Click on the valve position number to select the corresponding solvent stream to be used. Integrated stream switching parameter group: If this option is installed, select the position (1-6 or 1-2) of the high pressure ISS valve to be used. ProStar 430 Autosampler Control Manual - 34 - 03-914947-33:2...
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The Temperature circuit error led indicates if an error has occurred. NOTE: The temperature range for sample plate cooling is from 4 °C to 40 °C and the maximum cooling capacity is 20 °C below ambient temperature. ProStar 430 Autosampler Control Manual - 35 - 03-914947-33:2...
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Click on the button to move the right lift to standard operating position again. ProStar 430 Autosampler Control Manual - 36 - 03-914947-33:2...
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All tubing connected to the syringe valve will be filled in and rinsed with wash solvent. Not ready: contains the not ready information sent by the instrument. Events: contains the events and errors that have occurred on the instrument, listed below: ProStar 430 Autosampler Control Manual - 37 - 03-914947-33:2...
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The sample needle arm did not reach or leave destination within a certain time (horizontal). ERROR 34 Sample needle arm is not in vertical home position while moving horizontally. ERROR 39 Vial sensor sticks. ProStar 430 Autosampler Control Manual - 38 - 03-914947-33:2...
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Not enough transport liquid available due to missing transport vials. Electronics: ERROR 71 Flexprint of the sample needle is not connected. ERROR 72 Invalid configuration of the ProStar 430, PCB missing. ProStar 430 Autosampler Control Manual - 39 - 03-914947-33:2...
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Error grab head position. ERROR 95 Left lift no empty before run. ERROR 96 Horizontal or vertical position of grab head not reached in time. ERROR 97 Lift home time out. ProStar 430 Autosampler Control Manual - 40 - 03-914947-33:2...
Injection Mode Injection Principle A unique sequence of valve switching and syringe dispenser controlled sample withdrawal enables the ProStar 430 to inject sample volumes in a range from a few microliters to milliliters in a simple and reproducible manner. Therefore three different modes of injection can be selected :...
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The ProStar 430 uses a syringe to remove the sample from a vial into the sample loop. To prevent contamination of the syringe the ProStar 430 is equipped with buffer tubing between the syringe and the injection valve. The wash solvent is used to remove the sample from the buffer tubing and sample needle and rinse the buffer tubing and sample needle.
The head-space pressure, applied through the outer air needle, ensures that no air or vapor bubbles are formed during sample aspiration. Full loop injection: initial position. ProStar 430 Autosampler Control Manual - 43 - 03-914947-33:2...
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Full loop injection: flushing sample lines and needle. 3. The injection valve is switched into the Load position, placing a distinct sample front at the inlet of the sample loop. Full loop injection: switch injection valve to Load. ProStar 430 Autosampler Control Manual - 44 - 03-914947-33:2...
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5. The injection valve switches into the Inject position. The sample loop is now part of the HPLC mobile phase flow path: sample is transported to the column. The analysis starts. Full loop injection: Injecting sample. ProStar 430 Autosampler Control Manual - 45 - 03-914947-33:2...
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If more than one injection is to be made out of the same sample vial without performing a wash routine, after the analysis time has elapsed the ProStar 430 withdraws a flush volume to compensate for diffusion of mobile phase from the rotor groove into the front of the sample line during the analysis time.
The head-space pressure, applied through the outer air needle, ensures that no air or vapor bubbles are formed during sample aspiration. Partial loop injection: initial situation. ProStar 430 Autosampler Control Manual - 47 - 03-914947-33:2...
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Partial loop injection: flushing sample lines and needle. 3. The injection valve is switched into the Load position, placing a distinct sample front at the inlet of the sample loop. Partial loop injection: switch injection valve to Load. ProStar 430 Autosampler Control Manual - 48 - 03-914947-33:2...
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5. The injection valve switches into the Inject position. The sample loop is now part of the HPLC mobile phase flow path: sample is transported to the column. The analysis starts. Partial loop injection: injecting sample. ProStar 430 Autosampler Control Manual - 49 - 03-914947-33:2...
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This air segment is at the front of the flush volume and will not be injected. Situation after filling the loop in the case of partial loop injections with (A) or without air segment (B) ProStar 430 Autosampler Control Manual - 50 - 03-914947-33:2...
This to avoid disturbance of the chromatogram with an additional peak that could come from solvent that is different from the mobile phase. µL Pick-up injection: initial position. ProStar 430 Autosampler Control Manual - 51 - 03-914947-33:2...
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3. The needle moves from the mobile phase vial to the sample vial. The injection valve is switched into the Load position. µL Pick-up injection: go to sample vial and switch injection valve to Load. ProStar 430 Autosampler Control Manual - 52 - 03-914947-33:2...
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5. The sample needle moves back to the mobile phase vial. The sample is quantitatively transported into the loop, with mobile phase. µL Pick-up injection: transporting sample into the sample loop. ProStar 430 Autosampler Control Manual - 53 - 03-914947-33:2...
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µL Pick-up injection: injecting sample. For the next sequence the first withdrawal of mobile phase is omitted unless a wash routine is performed or the ProStar 430 has emptied the buffer tubing into the waste. In these cases the sequence is completely repeated.
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NOTE : In case of µL pick-up injections, there will be no air pressure (head-space pressure) on the sample vial, to avoid sample errors due to air expansion during vial exchange from the sample vial to the mobile phase vial. ProStar 430 Autosampler Control Manual - 55 - 03-914947-33:2...
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(Maximum speed 9 for 100 µL and 250 µL syringes, speed 6 for a 500 µL syringe and speed 4 for 1000 µL and 10 mL syringe.) If more viscous liquids are used the speeds should be reduced. ProStar 430 Autosampler Control Manual - 57 - 03-914947-33:2...