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BioSpectrum
Imaging System
®
™
Instruction Guide
______________________________________________________________________________
UVP, LLC
Ultra-Violet Products Ltd.
2066 W. 11th Street, Upland, CA 91786
Unit 1, Trinity Hall Farm Estate
Tel: (909) 946-3197 / (800) 452-6788
Nuffield Road, Cambridge CB4 1TG UK
Fax: (909) 946-3597
Tel: +44(0)1223-420022 Fax: +44(0)1223-420561
Web Site: www.uvp.com
81-0346-01 Rev F
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Summary of Contents for UVP BioSpectrum Imaging System
- Page 1 Ultra-Violet Products Ltd. 2066 W. 11th Street, Upland, CA 91786 Unit 1, Trinity Hall Farm Estate Tel: (909) 946-3197 / (800) 452-6788 Nuffield Road, Cambridge CB4 1TG UK Fax: (909) 946-3597 Tel: +44(0)1223-420022 Fax: +44(0)1223-420561 Web Site: www.uvp.com 81-0346-01 Rev F...
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Page 2: Table Of Contents
BioSpectrum Imaging System Table of Contents Introduction .................................. 3 Components ................................. 4 Darkroom Cabinet ..............................5 Five Position Filter Wheel ............................5 CCD Camera and Lens ............................. 5 UV-Safe Gel Viewer Window............................. 5 Lift Platform ................................5 Epi Illumination ................................6 LED White Light Illuminator ............................ -
Page 3: Introduction
BioSpectrum Imaging System Introduction The BioSpectrum Imaging System is designed to automate research with one-touch preset or user-defined PC controls for accurate, repeatable imaging and analysis of chemiluminescence, fluorescence, bioluminescence and colorimetric samples (depending upon configuration). The BioSpectrum incorporates a light-tight darkroom with VisionWorksLS software for automated control. -
Page 4: Components
BioSpectrum Imaging System Components The BioSpectrum Imaging System is comprised of the following equipment: Darkroom Cabinet Automated Filter Wheel and Emission Filters (3 Included with System) CCD Camera and Lens: MegaCam 810 with either 50mm f/1.2 Fixed, 30mm f/1.4 Fixed, or f/2.8 Zoom Lens OptiChemi™... -
Page 5: Darkroom Cabinet
BioSpectrum Imaging System Darkroom Cabinet The BioSpectrum Imaging System is constructed of aluminum and is fabricated to provide a light-tight chamber. Darkroom Dimensions: 17.5W x 17.5D x 32H inches (445 x 445 x 813 Note: The camera cover will add additional height which will vary based on camera application. -
Page 6: Epi Illumination
BioSpectrum Imaging System Epi Illumination Standard overhead lighting includes 365nm UV, Visi-Blue 480nm and white light. Lighting is controlled via the software interface. NOTE: The BioSpectrum includes an interlock switch that turns off all UV illumination, including the epi 365nm UV, when the darkroom door is opened. -
Page 7: Visionworks ® Ls Software
BioSpectrum Imaging System VisionWorks LS Software ® VisionWorksLS software controls the darkroom functions and lighting as well as the motorized lens and camera. The system may be configured with VisionWorksLS analysis software for analysis of gels, plates, blots and other samples. -
Page 8: Installation
If the computer is not connected to the Internet, select Offline activation and proceed to the following page of this manual, or call UVP to register the software. Click Next to continue. The Already have an activation ID option is useful when reloading the software after receiving an initial activation code. -
Page 9: Hardware Installation
BioSpectrum Imaging System If the computer is not connected to the Internet, click Offline activation to register the software. This allows the user to obtain the activation code and enter it at another time. Click Next to continue 10. Click the link provided and complete the form to obtain registration instructions. - Page 10 BioSpectrum Imaging System Darkroom Filter Setup Ensure that the darkroom is in a fixed and permanent location as filters may slide in movement. Locate the filter wheel in the darkroom’s camera well at the top of the darkroom, accessible when the camera and lens are removed.
- Page 11 BioSpectrum Imaging System Zoom Lens Controller Setup UVP Lens Controller Assemble the lens controller’s connective (pictured without devices as follows: camera and lens) Ensure that the zoom lens controller components and connections are assembled per the picture at right. Plug the power cable into the power Control Box supply.
- Page 12 BioSpectrum Imaging System Connecting to a PC and Powering Up the System Connect the USB cable from the computer to the rear exterior of the darkroom. Connect the camera to the computer and darkroom per the camera instructions in the back of this manual.
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Page 13: Camera Installation
High sensitivity, scientific grade CCD cameras are designed for use with UVP’s BioSpectrum Imaging Systems. This section covers the components and steps required to install and operate UVP’s cameras. Each camera has slightly different installation instructions, and the following cameras are covered in this manual: ... - Page 14 BioSpectrum Imaging System Hardware Installation Slide the camera and lens assembly into the camera base. The base comes installed to the darkroom from the UVP 90 degree USB factory. cable Plug the serial cable from the motorized lens into the darkroom.
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Page 15: Megacam 810, Optichemi 610 And Biochemi 510 Cameras
BioSpectrum Imaging System Software Installation Install the Camera Driver Plug in the 90 degree USB cable from the camera into the USB port. Ensure that the camera is connected to its own dedicated USB port and not to a USB hub or other similar shared device. - Page 16 The photograph shows the parts required for assembly of the MegaCam 810 and BioChemi 510 camera kits with the motorized lens on the BioSpectrum Imaging System. The OptiChemi 610 camera will have a different appearance but will be installed in the same manner.
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Page 17: Operation
BioSpectrum Imaging System Operation Using the System Caution: In BioSpectrum systems equipped with a motorized lift platform, do not place objects under the platform as these items will suffer damage if lift platform is moved to the lowest position. Note: The motorized lift platform (if equipped) is only functional if the darkroom door is closed. - Page 18 BioSpectrum Imaging System Camera Control The camera is controlled via VisionWorksLS software by clicking on the Acquisition Action Tab and then clicking on the Camera Menu Button. (Cameras have different functionality. Some options listed to the right in the Camera Menu may not appear for each camera. VisionWorksLS software automatically detects and enables the built-in functionality for each camera.)
- Page 19 BioSpectrum Imaging System Adjusting the Lift Platform Height BioSpectrum with Automated Lift Platform When using the Automated BioSpectrum, the lift platform height is adjusted from the Tray Height section of the Lighting Menu in VisionWorksLS. The lift platform adjusts to any location within a ten-inch travel range.
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Page 20: Capturing Images
BioSpectrum Imaging System Capturing Images Focusing the Lens Using Image Preview Place the sample or focus target on the surface where the sample will be imaged. Turn on the darkroom white light. Start the image preview by clicking on the Preview button within VisionWorksLS and move the sample around as necessary. -
Page 21: Service Procedures
BioSpectrum Imaging System Service Procedures Return Procedure A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service before returning any product. Troubleshooting No Power to the Darkroom Recheck main power cord connections to the darkroom. Check fuses, located at the back of the unit, near the power port. A flat-head screwdriver will be required. -
Page 22: Technical Support
PAGE gels, microplates and more. Technical Support UVP offers expert technical support on all UVP products If there are any questions about product use, operation or repair, contact UVP’s offices at the locations below. NOTE: A Returned Goods Authorization (RGA) number must be obtained from UVP’s Customer Service prior to returning any product.
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