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HB-500 Minidizer
Hybridization Oven
Instruction Guide
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UVP, LLC
Ultra-Violet Products Ltd.
2066 W. 11th Street, Upland, CA 91786
Unit 1, Trinity Hall Farm Estate
Tel: (909) 946-3197 / (800) 452-6788
Nuffield Road, Cambridge CB4 1TG UK
Fax: (909) 946-3597
Tel: +44(0)1223-420022 Fax: +44(0)1223-420561
Web Site: www.uvp.com
81-0169-02 Rev F
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Summary of Contents for UVP Minidizer HB-500
- Page 1 Ultra-Violet Products Ltd. 2066 W. 11th Street, Upland, CA 91786 Unit 1, Trinity Hall Farm Estate Tel: (909) 946-3197 / (800) 452-6788 Nuffield Road, Cambridge CB4 1TG UK Fax: (909) 946-3597 Tel: +44(0)1223-420022 Fax: +44(0)1223-420561 Web Site: www.uvp.com 81-0169-02 Rev F...
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Page 2: Table Of Contents
HB-500 Minidizer Hybridization Oven Table of Contents Introduction .................................. 3 Hybridization Oven Specifications ..........................4 Installation ..................................5 Operation ..................................6 Service Procedures ..............................7 Hybridization Techniques ............................10... -
Page 3: Introduction
HB-500 Minidizer Hybridization Oven Introduction The HB-500 Minidizer™ Hybridization Oven is a cost-effective, personal desktop hybridization oven with a capacity of four 35x150mm bottles, eight 50ml or 15ml conical plastic tubes. The Minidizer operates with a state-of-the-art microprocessor as well as temperature and variable speed controls. The HB-500 Minidizer enables consistent saturation of samples, whether it for washing or hybridizing. -
Page 4: Hybridization Oven Specifications
HB-500 Minidizer Hybridization Oven Hybridization Oven Specifications HB-500 Minidizer Hybridization Oven Part Number Volts/Hz 95-0330-01 115V/60Hz 95-0330-02 230V/50Hz 95-0330-03 100V/50Hz Specifications Net Weight: 11.3 lbs (5 kg) Temperature: Ambient +10°C to 80°C Heating Element: 500 watts Temperature Display: Rotation Speed: 12 RPM Bottle Capacity: Four (4) 35x150mm, Eight (8) 50ml, or Eight (8) 15ml... -
Page 5: Installation
HB-500 Minidizer Hybridization Oven Installation The Minidizer Hybridization Oven has capacity for four 35x150mm bottles and eight 50ml or 15ml conical plastic tubes. Combinations of multiple-sized bottles may be used at the same time. • To insert bottles or tubes into the carousel, gently press them into the clips. -
Page 6: Operation
The hybridization oven is calibrated at the UVP factory. UVP recommends temperature recalibration be performed at the UVP factory as recalibration by the user may void the warranty. To return the unit to UVP, first obtain a RGA (Returned Goods Authorization) number from UVP. -
Page 7: Service Procedures
• Clean the UV sensor regularly with alcohol and a soft cloth. Bottle Care UVP’s hybridization bottles are made of thick walled borosilicate glass which helps protect users from radiation and has excellent long-term reliability. • It is important to check the bottles regularly for chips, stress fractures and cracks. If this occurs, the bottle must be replaced. - Page 8 Power Cord (230V) Technical Support UVP offers expert technical support on all UVP products. If there are any questions about product use, operation or repair, contact UVP’s offices at the locations below. NOTE: A Returned Goods Authorization (RGA) number must be obtained from UVP’s Customer Service prior to returning any product.
- Page 9 Moreover, UVP makes no warranties whatsoever with respect to parts not supplied by UVP or that have been installed, used and/or serviced other than in strict compliance with the instructions appearing in the operational manual supplied to the end user.
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Page 10: Hybridization Techniques
HB-500 Minidizer Hybridization Oven Hybridization Techniques There are two main steps to a hybridization reaction: hybridizing two strands of complimentary DNA, and detection of the hybridized DNA. Nucleic acid hybridization is a mechanism where strands of DNA in a single stranded state have their complements bind together. - Page 11 HB-500 Minidizer Hybridization Oven 3: Salt Concentration and Temperature Nucleic acid requires salt (monovalent cations) to reduce the ionic effects of the phosphate backbone, and heat as a form of non-denaturing kinetic energy. Because the salt concentration and temperature affect each other, knowing the thermostability of the hybrid probe is helpful.
- Page 12 Notes: When preparing prehybridization/hybridization solutions, add dry reagents directly to the formamide/SSC solution. Incubate with mixing at 40-50°C for 2 hours or until dissolved. Store at -20°C. SDS will precipitate at room temperature but remain in solution at 37°C. • UVP Hybridization bottle(s) and caps • 15mL plastic tube...
- Page 13 AVOID pouring the probe directly onto the blot. Incubate in the UVP HybriCycler, Hybridizer, or Minidizer 6 to 8 hours at 42 to 56°C. Washing the Blot •...
- Page 14 Ross, J; Nucleic Acid Hybridization: Essential Techniques; 1998, John Wiley and Sons, ISBN 0-471- 97125-1 Sambrook, J; Molecular cloning: a Laboratory manual; 1987, Cold Spring Harbor Laboratory Press; ISBN 0-87969-309-6 Current Protocols in Molecular Biology; 1987, John Wiley and Sons; ISBN 0-471-50338-X Minidizer is a trademark of UVP, LLC.
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