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Summary of Contents for Cardinal Health CHB-AGS
- Page 1 Cardinal Health™ Single Slide Automated Gram Stainer Instructions for Use Manual CHB-AGS CHBAGS_GramStainer_IFU.indd 1 7/3/18 1:31 PM Art specifications: IFU/Manual - 8"Wx10"H Do not alter art or size. All art prints PMS 185 Red and 100% Black. AMS1800062 Rev. 0 2018-06...
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Page 2: Table Of Contents
Table of Contents Specifications ..................................5 1.0 INTRODUCTION ................................6 2.0 WORK AREA REQUIREMENTS .............................7 3.0 RECEIVING AND UNPACKING ............................7 4.0 MATERIALS REQUIRED BUT NOT PROVIDED .......................7 5.0 GRAM STAIN KITS ..............................7 5.1 General Information ..............................7 5.2 Ordering Information ..............................7 5.3 Reagent Kit Installation ............................7 6.0 OPERATIONS INSTRUCTIONS ............................8 6.1 Instrument Anatomy ..............................8 6.2 Specimen Slide Preparation ............................8... - Page 3 9.0 MAINTENANCE ................................14 9.1 Wash Cycle ................................14 9.2 Bleach Cycle ................................15 9.3 Scrub Cycle ................................16 9.4 Cuvette Swabbing ..............................17 9.5 Deionized Water Disinfectant ............................17 9.6 Refilling the Water Supply ............................18 9.7 Maintenance Summary .............................18 10.0 IDLE DELAYS ................................18 10.1 Setting the Sleep Delay ............................19 11.0 UNIT SHUT DOWN..............................19 11.1 Unit Shut Down Instructions ...........................19 12.0 SERVICES AND SUPPLIES ............................20...
- Page 4 Congratulations! You have made an excellent choice for your Lab. Cardinal Health™ thanks you for the trust you have placed in our products and services. This operating manual has been designed to help you gain an understanding of the operation and application of our Single Slide Automated Gram Stainer.
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Page 5: Specifications
SPECIFICATIONS Dimensions/Weight Width 30.5 cm (12.0 in.) Height 40.6 cm (16.0 in.) Depth 33.0 cm (13.0 in.) Weight 12.24 Kg (27.0 lbs.) Power Requirements Input: 100-240 VAC, 8-4A, 47-63Hz Temperature, Ambient Operation 15-30C Humidity 0 to 95%, without condensation Operator Adjustments Individual adjustable stain times on both short and long cycles Adjustable sleep mode Adjustable audible sound volume control... -
Page 6: Introduction
INTRODUCTION This Operation Manual is provided to guide the user in all aspects of unit set-up, operational use, and user-level maintenance of the Cardinal Health™ Single Slide Automated Gram Stainer unit. The Single Slide Automated Gram Stainer is a microscope slide stainer capable of automatically performing a Gram stain sequence on a slide containing biological specimen for in vitro diagnostic use. -
Page 7: Work Area Requirements
The Single Slide Automated Gram Stainer unit requires a level counter top surface of a minimum of 12 inches wide by 13 inches deep with a height clearance of 16 inches. The Gram Stain Kit (Cat. No. CHB-AGS-SK) and a container filled with deionized water should be positioned at the same level as the instrument immediately on the left side connected by the tubing coming out of the machine. -
Page 8: Operations Instructions
The chance of this can be minimized by carefully following the instructions in this manual. NOTE: For best results with this instrument use glass slides that are positively charged such as Cardinal Health™ Microscope Slide Charged Adhesion Frosted 25mm x 75mm 1mm (Cat. -
Page 9: Instrument Preparation
6.3.1 Remove the screws to open the front panel that is covering the pump tube rollers, and attach all eight orange pump tubes around their pump rollers. When installing a new pump Replacement Tube kit (Cat. No. CHB-AGS-RT) refer to section 8.2 for detailed instructions. - Page 10 6.4.4 Press [1] on the key pad to select GRAM STAIN. Reagent volume and expiration dates are checked at this time to assure staining operation may be completed. Detection of reagent shortage or expiration yields a warning message describing the reagent status. NOTE: The Gram Stain command will fail to start if the instrument is due for a required Bleach or Scrub cleaning cycle.
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Page 11: Quality Control
NOTE: Do not allow the slide to drop and abruptly hit the “Slide Stop” within the cuvette because the slide can chip or break. Broken glass in the instrument is not covered under warranty and the customer will be responsible for repairs and shipping. For assistance in the case of a broken slide, call Cardinal Health™... -
Page 12: Pump Replacement Tube Procedure
8.1.5 Open the new Gram Stain Kit and insert the labeled cannula into the corresponding reagent bottles. 8.1.6 From the Main Menu, Press [ 2 ] on the key pad to select SET-UP. 8.1.7 Press [ 2 ] on the key pad to select Consumables 8.1.8 Press [ 1 ] on the key pad to select Review Reagents. -
Page 13: Register New Pump Replacement Tube Kit
8.2.5 Remove the two white nylon elbows from each of the orange pump tubes. Nylon Elbows Nylon Elbows 8.2.6 Remove the orange pump tube from the pump roller and bracket. 8.2.7 Install the new orange pump tube into the bracket and onto the roller. 8.2.8 Insert the two nylon elbows into the new pump tube. -
Page 14: Maintenance
9.0 MAINTENANCE 9.1 Wash Cycle The Wash feature for the Single Slide Automated Gram Stainer is required every 20 slides or every 8 days. The unit runs water and decolorizer through the cuvette and with agitation cleans the internal surface of the cuvette and tubing. 9.1.1 Press [ 1 ] on the key pad to select Run 9.1.2 Press [ 2 ] on the key pad to select Clean Cuvette 9.1.3 Press [ 1 ] on the key pad to select Wash Cuvette... -
Page 15: Scrub Cycle
9.2.12 Press [ 2 ] on the key pad to select Gram Stain (LONG) 9.2.13 Place a clean, blank slide in the cuvette when the screen prompt reads Load Slide 9.2.14 Press any key on the key pad, except [ * ], to commence the Bleach Cycle 9.2.15 When the cycle is complete, Press [ * ] on the key pad to return to the Main Menu 9.2.16 Press [ 1 ] on the key pad to select Run 9.2.17 Press [ 4 ] on the key pad to select Purge... - Page 16 9.3.4 Press [ 1 ] on the key pad to select Purge, this will empty the lines of stains. 9.3.5 Move the Crystal Violet, Gram’s Iodine, and Safranin cannulas into one bottle of System Cleaner (Cat. No. CHB-AGS-SC), or a bottle containing at least 500 mL of Decolorizer.
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Page 17: Cuvette Swabbing
9.4 Cuvette Swabbing The cuvette may build up stain residue over time. To clean it, use a foam cleaning swab (Cat. No. CHB-GS-CS) soaked in alcohol to scrub the walls of the cuvettes. This should be done while the instrument is not in Run Mode, but while a Scrub Cycle is in process. Scrub the cuvette walls with a clean foam swab while decolorizer and water is rinsing the chamber. -
Page 18: Idle Delays
10.0 IDLE DELAYS The Single Slide Automated Gram Stainer contains an internal clock that is used to monitor system operations and start a timer to manage the instruments “sleep” time to conserve energy. The software monitors the idle time by observing the elapsed time since the last key on the key pad was pressed. To set a sleep time that works best for your lab, refer to Section 10.1 below. -
Page 19: Unit Shut Down
Then, press any key to continue. NOTE: For the Scrub procedure it is recommended that the System Cleaner for Single Slide Automated Gram Stainer (Cat. No. CHB-AGS-SC) is used. Alternatively, decolorizer can be used if there is no available system cleaner. -
Page 20: Services And Supplies
12.0 SERVICES AND SUPPLIES If a problem is encountered that is beyond the scope of this manual or additional assistance is required, contact our Cardinal Health™ Technical Support Team serviced by QuickSlide™ at (800) 266-2222 (ext. 2). Additional supplies for your Single Slide Automated Gram Stainer can be purchased through Cardinal Health. - Page 21 15.0 Single Slide Automated Gram Stain Warranty Cardinal Health utilizes Hardy Diagnostics to repair or replace the instrument under the terms and conditions of this warranty. The liability of Hardy Diagnostics under this warranty, whether in contract, tort, or otherwise, shall not, except as expressly provided herein, exceed Buyer’s purchase price on which such liability is based.
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Page 22: Single Slide Automated Gram Stainer Warranty
16.0 Maintenance Logs Single Slide Automated Gram Stainer User Maintenance Log When refilling the 20 slides or Every 15 days or every 600 slides, 6 Months or every 1,100 cycles, water container or every 8 days whichever occurs first whichever occurs first every 20 days whichever comes first Deionized Water and... -
Page 23: Single Slide Automated Gram Stainer Training Checklist
Trainee Name: Single Slide Automated Gram Stainer Training Checklist Refer to the user manual for each item on this checklist. Check the box for each item trained. Trainee’s Initials _____ Specimen Slide Preparation: Section 6.2 _____ Loading Slides: Section 6.4.6 _____ Initiating a Gram Stain: Section 6.4 _____... - Page 24 2018-06 cardinalhealth.com CHB-AGS © 2018 Cardinal Health. All Rights Reserved. CARDINAL HEALTH and the Cardinal Health LOGO are trademarks of Cardinal Health and may be registered in the US and/or in other countries. CHBAGS_GramStainer_IFU.indd 24 7/3/18 1:31 PM Art specifications: IFU/Manual - 8"Wx10"H...
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