To Check Ecd Performance - Agilent Technologies 8890 Operation Manual

17 Chromatographic Checkout

To Check ECD Performance

1 Gather the following:
2 Verify the following:
3 Replace consumable parts (liner, septum, traps, syringe, and so forth) as needed for the
4 Install the evaluation column.
5 Display the signal output to determine baseline output. A stable baseline output at any
6 Create or load a method with the parameter values listed in
Table 45 ECD Checkout Conditions
Column and sample
Type
Sample
Column mode
Column flow
252
• Evaluation column, HP-5 30 m × 0.32 mm × 0.25 µm (19091J-413)
• ECD performance evaluation (checkout) sample (18713–60040, Japan: 5183-0379)
• 4-mL solvent and waste bottles or equivalent for autoinjector.
• Chromatographic-grade isooctane
• 2-mL sample vials or equivalent for sample.
• Inlet and injector hardware See
• Clean fused silica indented mixing liner installed. If not, install it.
• Chromatographic-grade gases plumbed and configured: helium for carrier gas, nitrogen
for makeup.
• Empty waste vials loaded in sample turret.
• 4-mL vial with diffusion cap filled with hexane and inserted in Solvent A injector position.
checkout. See "To Prepare for Chromatographic
• Bake out the evaluation column for at least 30 minutes at 180 °C.
• Be sure to configure the column.
value between 0.5 and 1000 Hz (OpenLAB CDS ChemStation Edition display units)
(inclusive) is acceptable.
• If the baseline output is < 0.5 Hz, verify that the electrometer is on. If the offset is still
< 0.5 Hz, your detector requires service.
• If the baseline output is > 1000 Hz, there may be chemical contamination contributing to
the signal. Bakeout the
check gas purity. Use higher purity gases and/or install traps.
"To Prepare for Chromatographic
Checkout."
ECD. If repeated cleanings do not give an acceptable signal,
HP-5, 30 m × 0.32 mm × 0.25 µm
(19091J-413)
ECD checkout (18713-60040 or Japan:
5183-0379)
Constant flow
6.5 mL/min (helium)
Checkout."
Table 45.
Operation Manual