Agilent Technologies AssayMAP Bravo Installation Manual page 104

Verifying the installation and setup
Performing a mock run
Protocol step
Internal Cartridge
Wash 1
Cup Wash 2
Internal Cartridge
Wash 2
Stringent Syringe
Wash
Elute
Final Syringe Wash
96
Head moves to Bravo Platform action
deck location...
5 Aspirates Cartridge Wash Buffer 1 into the syringes.
2 Mounts the cartridges on the head.
1 Dispenses 5 µL Cartridge Wash Buffer 1 through the
cartridges at the Load Samples flow rate for the Sample Chase
step.
1 Dispense Cartridge Wash Buffer 1 through the cartridges for
the Internal Cartridge Wash step.
1 Wash the exterior of the cartridge tips at the wash station.
2 Park the cartridges at the seating station.
1 Wash the syringes at the wash station.
6 Aspirate the Cartridge Wash Buffer 2 into the syringes.
2 Performs Cup Wash.
1 Dispenses the buffer at the wash station.
1 Washes the syringes at the wash station.
6 Aspirates the Cartridge Wash Buffer 2 into the syringes.
2 Mounts the cartridges on the head.
1 Dispenses the Cartridge Wash Buffer 2 through the cartridges
for the Internal Cartridge Wash 2 step.
1 Washes the exterior of the cartridge tips at the wash station.
2 Parks the cartridges at the seating station.
1 Washes the syringes at the wash station.
8 Aspirates the Stringent Syringe Wash Buffer (Elution Buffer).
1 Dispenses the buffer at the wash station.
1 Washes the syringes at the wash station.
8 Aspirates the Elution Buffer.
2 Mounts the cartridges.
9 Elutes the samples into the Eluate Collection plate.
1 Washes the cartridge exteriors at the wash station.
2 Parks the cartridges at the seating station.
9 Mixes the eluates.
1 Washes the syringes at the wash station.
1 Washes the syringes at the wash station.
AssayMAP Bravo Platform Installation Guide