horiba 5002S-10C Instruction Manual
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horiba 5002S-10C Instruction Manual

horiba 5002S-10C Instruction Manual

Ammonia electrode
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Instruction Manual
Ammonia Electrode
5002S-10C
ELECTRODE

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Summary of Contents for horiba 5002S-10C

  • Page 1 Instruction Manual Ammonia Electrode 5002S-10C ELECTRODE...
  • Page 2 Also safely store the manual so it is readily available whenever necessary. Please contact HORIBA Advanced Techno if any points regarding safety come to your attention during use. Product specifications and appearance, as well as the contents of this manual are subject to change without notice.
  • Page 3  China regulations 标记的意义 Meaning of Marking 本标记适用在中华人民共和国销售电器电子产品， 标记中央的数字表示环 境保护使用期限的年数。 (不是表示产品质量保证期间。 ) 只要遵守这个产 品有关的安全和使用注意事项， 从制造日开始算起在这个年限内， 不会给环 境污染、 人体和财产带来严重的影响。 请不要随意废弃本电器电子产品。 This marking is applied to electric and electronic products sold in the People's Republic of China. The figure at the center of the marking indicates the environmental protection use period in years.
  • Page 4  For your safety  Hazard classification and warning symbols Warning messages are described in the following manner. Read the messages and follow the instructions carefully.  Hazard classification This indicates an imminently hazardous situation which, if not avoided, will result in death or serious injury.
  • Page 5  Safety precautions This section provides precautions for using the product safely and correctly and to prevent injury and damage. The terms of DANGER, WARNING, and CAUTION indicate the degree of imminency and hazardous situation. Read the precautions carefully as it contains important safety messages. Precautions for chemicals Solutions used for calibration and measurement must not come in contact with the skin, or consumed.
  • Page 6  Do not touch a hard surface with the electrode. It may cause a failure.  Only use the product including accessories for their intended purpose. HORIBA Advanced Techno assumes no liability when they are used for any other products.
  • Page 7  Manual information  Description in this manual The following explains the icons used in this manual. Icon Meaning This interprets the necessary points for correct operation and notifies the important points for handling the product. Note Note This indicates reference information. This indicates the part where to refer for information.

  • Page 9: Table Of Contents

    ontents Before Use Confirmation of the items included in the package ..2 About this product ....... . 3 Measurement procedure .
  • Page 10 Maintenance Storage ........38 Consumables .
  • Page 11 igures Fig. 1 Included items ......2 Fig. 2 System configuration..... . 3 Fig.
  • Page 12 ables Table 1 Ammonium ion concentration measurement unit conversion table ....30 Table 2 Check list ......48 Table 3 Relationship between temperature of ion selective electrode and theoretical slope .

  • Page 13: Before Use

    efore Use This chapter describes what you should know before using the product. Confirmation of the items included in the package ........2 About this product ......3 Product features............3 System configuration ...........3 Names of parts.............4 Measurement procedure ....5...

  • Page 14: Confirmation Of The Items Included In The Package

    onfirmation of the items included in the package Open the package and make sure that the following items are included. 1000 mg/L Standard solution (50 mL) Main unit 100 mg/L Standard solution (50 mL) of electrode Instruction manual Syringe for (this document) Internal solution adjusting pH...

  • Page 15: About This Product

    This minimizes the interference on the electrode potential caused by the entry of gas into the internal solution.  System configuration This product is used with the meter of HORIBA Advanced Techno connected, which is sold separately. Electrode stand(*) is also available. Desktop type...

  • Page 16: Fig. 3 Names Of Parts

     Names of parts This product consists of the parts shown in the figure below. Cable Electrode cap Internal solution fill port Glass electrode for pH measurement Liquid junction of glass electrode for pH measurement Electrode connector (BNC connector) Membrane cap Support pipe Protective cap pH glass ion selective...

  • Page 17: Measurement Procedure

    easurement procedure The following figure shows the procedure for measuring the ammonium ion concentration. Measurement preparation Rinse with pure water. page 11) Fill with internal solution. page 10) Attach the membrane cap to the support pipe. page 9) Connect the electrode to the meter.
  • Page 18 Calibration Measurement Prepare two types of Prepare the sample solution. solutions for calibration. page 29) page 15) Perform calibration. page 18) In the following cases, perform conditioning of the electrode before Perform measurement. calibration. page 30)  Before the first use ...

  • Page 19: Electrode Preparation

    lectrode Preparation This chapter describes the preparation that must be performed before using the product. Assembling the electrode ....8 Rinsing the electrode..... 12 Connecting the electrode to the meter ..........13...

  • Page 20: Assembling The Electrode

     Be sure to fill with internal solution before use.  Use the internal solution included in the package or available as a consumable sold Note Note separately by HORIBA Advanced Techno. “Consumables” (page 66)) Items to be prepared Internal Syringe for Main unit...
  • Page 21 Remove the protective cap. Protective cap Take care to ensure that the internal solution in the protective cap does not come out while removing. Note Note When disposing of the standard solution in the protective cap, follow the local rules and regulations. Wipe off the moisture.
  • Page 22 Firmly attach the membrane cap by turning it clockwise. Open the rubber stopper of the Rubber stopper internal solution fill port. Fill the internal solution with the syringe until it comes up to the line Internal solution shown in the figure with arrow. fill port...
  • Page 23 Close the rubber stopper of the internal solution fill port.  Before use, leave the membrane cap attached on the electrode soaked in the beaker filled with the internal solution for 15 minutes or longer. ( "Conditioning of the electrode" (page 21)) ...

  • Page 24: Rinsing The Electrode

    insing the electrode Make sure that no dirt and other foreign matters have View video View video adhered to the electrode before measurement. If dirt and other foreign matters have adhered to the electrode, rinse the electrode as described in the following procedure.

  • Page 25: Connecting The Electrode To The Meter

    onnecting the electrode to the meter Connect the electrode to the meter. View video View video For details on the connector of the meter to which the electrode will be connected, refer to the instruction manual of the meter. Connect the electrode connector to the meter. When disconnecting the electrode from the meter, disconnect it in the opposite direction from step 1.

  • Page 27: Calibration

    alibration This chapter describes calibration. Standard solutions......16 Calibration operation ..... 17...

  • Page 28: Standard Solutions

    The following standard solutions are used for calibration and measurement.  1000 mg/L NH standard solution Use the standard solution included in the package or available as consumables by HORIBA Advanced Techno.  100 mg/L NH standard solution Use the standard solution included in the package or available as consumables by HORIBA Advanced Techno.

  • Page 29: Calibration Operation

    alibration operation Measure standard solutions in ascending order of ion concentration to create calibration curves in the meter ( “Ammonia electrodes” (page 54)). Perform calibration once every day or before measurement as appropriate. Before operation  Be sure to fill with internal solution and rinse the electrode before the calibration operation.
  • Page 30  Prepare at least two types of standard solution with different concentrations.  Always use new standard solution for calibration.  After the standard solutions (50 mL) Note Note included in the package is used up, use the standard solution (500 mL) available as consumables.
  • Page 31 Prepare the first solution for calibration. (1) Put 50 mL of 100 mg/L standard solution into a 100 mL beaker. (2) Add 1 mL of pH-adjusting ISA. (3) Stir the solution slowly with a magnetic stirrer. (1) 100 mg/L standard solution (2) pH-adjusting ISA 50 mL 1 mL...
  • Page 32 Prepare the second solution for calibration. (1) Put 50 mL of 1000 mg/L standard solution into a 100 mL beaker. (2) Add 1 mL of pH-adjusting ISA. (3) Stir the solution slowly with a magnetic stirrer. (1) 1000 mg/L standard solution (2) pH-adjusting ISA 50 mL 1 mL...
  • Page 33 Conditioning of the electrode Soaking the electrode in a internal solution is called conditioning. In the following Internal solution cases, perform the conditioning for 15 minutes or longer before calibration.  Before the first use  Before use after storage for one week or Note Note longer...
  • Page 34 Shake the electrode to remove any air bubbles remaining on the membrane. Shake the electrode View video View video  If air bubbles remain on the membrane, it may result in inaccurate measurement.  Do not hit the beaker with the Note Note electrode.
  • Page 35 Rinse the electrode with pure water. Make sure that the membrane part has been well rinsed with pure water. Wipe off the moisture. Wipe off the moisture using soft and clean lint-free paper or cloth. Soak the electrode in the second solution for calibration prepared in step 4.
  • Page 36 Shake the electrode to remove any air bubbles out of the membrane. Shake the electrode View video View video  If air bubbles remain on the membrane, it may result in inaccurate measurement.  Do not hit the beaker with the Note Note electrode.
  • Page 37 Check that slope is at least 80%. If the slope is less than 80%, the measurement may not be performed accurately. The possible causes of calibration failure are:  Membrane is dirty or broken  Change in the internal solution concentration ...

  • Page 39: Measurement

    easurement This chapter describes measurement. Requirements for sample and standard solutions ......28 Measurement hints ......29 Measurement units ......30 Measurement operation....31...

  • Page 40: Requirements For Sample And Standard Solutions

    equirements for sample and standard solutions The requirements for sample solutions and standard solutions are as follows:  The temperatures of sample solution and standard solution shall be kept at 50°C or less. Exceeding 50°C may cause electrode deterioration.  The standard solution in calibration and the sample solution in measurement shall have almost the same temperature.

  • Page 41: Measurement Hints

    easurement hints  A gradual rise in solution temperature due to the magnetic stirrer generating heat may cause an apparent potential drift. When it happens, reduce the influence of the temperature changes by covering the magnetic stirrer with heat insulation such as polystyrene foam or using a thermostatic bath.

  • Page 42: Measurement Units

    easurement units This product can measure ammonium ion concentration in three types of units. Refer to the following table to convert the measurement unit. Table 1 Ammonium ion concentration measurement unit conversion table Ammonium ion Ammonium ion Ammonia nitrogen concentration concentration concentration [mol/L]...

  • Page 43: Measurement Operation

    easurement operation Before operation  The calibration characteristics vary depending on the temperature. The standard solution in calibration and the sample solution in measurement shall have almost the same temperature. Match the temperature as close as possible by, for example, using a thermostatic bath. The temperature difference may cause measurement errors.
  • Page 44 Prepare the sample solution. In this section, the case using sample solution (250 mg/L) is used as an example in the following steps. (1) Put 50 mL of sample solution (concentration of 250 mg/L) in a 100 pH-adjusting ISA: 1 mL mL beaker.
  • Page 45 Wipe off the moisture. Wipe off the moisture using soft and clean lint-free paper or cloth. Soak the electrode in the sample solution prepared in step 1. Soak the membrane cap fully into the sample solution. Do not touch the stir bar with the electrode. It may damage the electrode.
  • Page 46 View video View video  If air bubbles remain on the membrane, it may result in inaccurate measurement.  Do not hit the beaker with the Note Note electrode. It may result in inaccurate measuring. Perform the measurement. Wait for a stable reading and measure the ion concentration as described in the Meter Instruction Manual.

  • Page 47: After Measurement

    fter Measurement This chapter describes how to keep the electrode after measurement. How to keep the electrode..... 36...

  • Page 48: How To Keep The Electrode

    ow to keep the electrode Make sure the electrode used for measurement is kept soaked in the internal solution until the next measurement is started. When the electrode will not be used for one week or longer, store it as described on page 38. Note Note...

  • Page 49: Maintenance

    aintenance This chapter describes the storage procedure of the electrode as well as the lifespan and replacement procedure of each part. Storage..........38 Main unit of electrode..........38 Consumables........41 Consumables and replacement periods ....41 Membrane cap replacement..43...

  • Page 50: Storage

    torage When the electrode will not be used for one week or longer, store it as described in the following procedure.  Main unit of electrode Turn the electrode with the membrane cap upward and remove the membrane cap by turning it counterclockwise.
  • Page 51 Close the rubber stopper of the internal solution fill port. Rinse the membrane cap and the electrode with pure water.
  • Page 52 Wipe off the moisture. Wipe off the moisture using soft and clean lint-free paper or cloth. Make sure that there are no dirt or crystals of internal solution adhered to the area around the glass electrode for pH measurement and support pipe. If dirt remains, repeat steps 4 and 5.

  • Page 53: Consumables

    onsumables  Consumables and replacement periods The consumables that need to be replaced and the replacement periods are as follows. Replace each part at the appropriate timing to ensure accurate measurement. approx. 1 month approx. 1 month approx. 1 month Replace Service life Membrane cap...
  • Page 54 Single piece of the electrode main unit is not available for sale. When replacing the main unit of electrode, purchase the ammonia electrode including the membrane cap Note Note (3200698386). Use this up before the expiration date displayed on the bottle. Standard solution Use this up before the expiration date displayed on the bottle.

  • Page 55: Membrane Cap Replacement

     Membrane cap replacement Replace the membrane cap as described in the following procedure. Turn the electrode with the old membrane cap upward and remove the old membrane cap by turning it counterclockwise. When disposing of the old membrane cap, follow the local rules and regulations.
  • Page 56 Fill the internal solution inside until it reaches around 1 mm height inside the membrane cap. Firmly attach the membrane cap by turning it clockwise. Fill the internal solution with the syringe until it comes up to the Internal solution line shown in the figure with fill port arrow.
  • Page 57 Close the rubber stopper of the internal solution fill port.  Before use, leave the membrane cap attached on the electrode soaked in the beaker filled with the internal solution for 15 minutes or longer. ( "Conditioning of the electrode" (page 21)) ...

  • Page 59: Troubleshooting

    roubleshooting This chapter describes the measures to take when accurate measurement is not possible. When accurate measurement is not possible........... 48...

  • Page 60: Table 2 Check List

    hen accurate measurement is not possible When accurate measurement is not possible, check the following. Table 2 Check list Check item Solution View in video Are the temperatures The standard solution in of the standard calibration and the sample solution and sample solution in measurement shall solution the same? have almost the same...
  • Page 61 Check item Solution View in video Is the temperature of A gradual rise in solution the solution being temperature due to the magnetic stirred rising? stirrer generating heat may cause an apparent potential drift. Reduce the influence of the temperature changes by covering ―...
  • Page 62 Check item Solution View in video Has the internal Replace the internal solution solution been before each measurement. replaced? If not, the internal solution concentration will change and it will cause measurement error. Is there a sufficient Make sure that the surface of the amount of internal solution is just below the internal solution?
  • Page 63 Check item Solution View in video Has the standard If the standard solution has solution expired? expired, replace it with a new one. Use up the standard solution within approximately 3 months after opening the bottle. Do not ― return the standard solution to its bottle once it has come out of the bottle.
  • Page 64 Check item Solution View in video Has the membrane Attach the membrane cap cap been attached properly. If the membrane cap is properly? loose, the standard solution or sample solution may enter inside ― the electrode resulting in a measurement error. “Assembling the electrode”...

  • Page 65: Reference

    eference This chapter describes general matters regarding ammonia electrodes. Ammonia electrodes...... 54 Characteristics of ammonia electrode ......... 57 Measurement range...........57 Impact of pH...............58 Impact of temperature ..........58 Impact of stirring............59 Impact of coexistent substances ........60 Response time ............60 Standard solutions .............61...

  • Page 66: Fig. 4 Structure Of Ammonia Electrode

    mmonia electrodes The structure of the ammonia electrode is shown in Fig. 4. The ammonia electrode is configured by a glass electrode for pH measurement, a support pipe, and a membrane cap. The tip of the glass electrode for pH measurement is made of a pH glass ion selective membrane, and the tip of the support pipe has a membrane cap provided with a membrane attached to it.

  • Page 67: Fig. 5 Abundance Ratio Between

    The abundance ratio between the ammonium ion NH and ammonia NH based on the pH value is shown in Fig. 5. It is understood that when the pH value becomes 12 or more (alkalinity), most of the ammonium ions NH convert to ammonia NH ammonia NH (gases dissolved in...
  • Page 68 The ammonia NH that has been converted to gas permeates through the membrane, and disassociates into ammonium ions NH and hydroxide ions in the internal solution. (Gas) + H + OH … (2) The expression shown below can be obtained if the disassociation constant is assumed to be K.

  • Page 69: Fig. 7 Calibration Curve Of Ammonia Electrode

    haracteristics of ammonia electrode  Measurement range The range where linearity is established between the logarithm of the ion concentration and the electromotive force is called the measurement range. In general, the range for measuring ion concentration using ammonia electrodes is a maximum of around 110 mol/L and a minimum of around 110 mol/L to 110...

  • Page 70: Table 3 Relationship Between Temperature Of Ion Selective Electrode And Theoretical Slope

     Impact of pH The ammonia electrode can be used in the specified pH range. The impact of the pH may cause a decrease in the slope of the ammonia electrode, or parallel shifting of the calibration curve. To prevent these effects, add an appropriate amount of pH-adjusting ISA to keep the pH of the solution constant in advance.

  • Page 71: Table 4 Relationship Between Temperature Of Ammonia Electrode And Theoretical Slope

    The optimal way to minimize the impact of temperature on the measurement of the ammonium ion concentration is to reduce the difference between the temperature of the standard solution in calibration and the temperature of the sample solution in measurement to ±1°C. The following table shows the change in the theoretical slope of the ammonia electrode depending on the temperature of the ammonia electrode.

  • Page 72: Impact Of Coexistent Substances

     Impact of coexistent substances Because ammonia electrode determines ammonia concentration by measuring pH change in inner solution caused by ammonia gas permeating through the membrane, presence of acidic or basic gas other than ammonia causes error. Such acidic gas as H S or CO , however, is ionized in alkali region and does not exist as acidic gas, so that no problem...

  • Page 73: Standard Solutions

     Standard solutions  Selecting standard solutions  Perform calibration using standard solutions of 100 mg/L and 1000 mg/L. Approximate concentrations can be measured.  For more accurate measurements, prepare standard solutions which have the following concentration relationship for calibration. Standard solution with low concentration <...

  • Page 75: Material

    aterial This chapter describes the specifications, external dimensions, and consumables of the product. Specifications......... 64 External dimensions ...... 65 Consumables........66...

  • Page 76: Table 5 Specifications Of Ammonia Electrode

    pecifications The following table shows the specifications of the product. Table 5 Specifications of ammonia electrode Item Description Operating temperature range 0°C to 50°C Internal electrode Ag/AgCl (silver/silver chloride) electrode Internal solution 0.1 mol/L NH Wetted part (part contacting PTFE, PVC, PSU sample solution) material Measurement range 0.01 mg/L to 18000 mg/L as NH...

  • Page 77: External Dimensions

    xternal dimensions 16.5 O.D. [Unit: mm]...

  • Page 78: Table 6 Consumables

    onsumables Consumables can be purchased from your nearest local representative by notifying the product name and the part number. Table 6 Consumables Specifica- Product name Model Part number tions Ammonia electrode 5002S 3200698386 -10C Ammonia electrode Membrane 3200705774 membrane cap (3 units) 1000 mg/L 500 mL...
  • Page 79 31, Miyanonishi-cho, Kisshoin Minami-ku, Kyoto 601-8306, Japan http://www.horiba-adt.jp For any questions regarding this product, please contact your local agency, or inquire from the following website. http://global.horiba.com/contact_e/index.htm...

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