ProteinSimple Maurice S User Manual

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User Guide for Maurice, Maurice

C. and Maurice S.

ProteinSimple

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San Jose, CA 95134

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Page 1 1 User Guide for Maurice, Maurice C. and Maurice S. Copyright © 2018 ProteinSimple. All rights reserved. ProteinSimple 3001 Orchard Parkway San Jose, CA 95134 Toll-free: (888) 607-9692 Tel: (408) 510-5500 Fax: (408) 510-5599 email: support@proteinsimple.com web: www.proteinsimple.com...
Page 2 For research use only. Not for use in diagnostic procedures Patents and Trademarks ProteinSimple's Maurice and iCE technology is covered by issued and pending patents in the U.S. and other countries. For more information please see http://www.proteinsimple.com/Intellectual_Property.html. ProteinSimple and the ProteinSimple logo are trademarks or registered trademarks of ProteinSimple. Other...
Page 3: Table Of Contents
Viewing Release Notes .......33 Launching Compass for iCE ......19 User Guide for Maurice, Maurice C. and Maurice S.
Page 4 Importing a Method ......72 User Guide for Maurice, Maurice C. and Maurice S.
Page 5 Viewing Run Errors....... . . 205 User Guide for Maurice, Maurice C. and Maurice S.
Page 6 Exporting Results Tables..... . . 267 Analysis Screen Panes......240 User Guide for Maurice, Maurice C. and Maurice S.
Page 7 Creating a New Standards Group ....301 Analysis Screen Panes ......337 User Guide for Maurice, Maurice C. and Maurice S.
Page 8 Peak Fit Analysis Settings ......397 User Guide for Maurice, Maurice C. and Maurice S.
Page 9 Run File History ........465 User Guide for Maurice, Maurice C. and Maurice S.
Page 10 Reagents........489 User Guide for Maurice, Maurice C. and Maurice S.
Page 11: Let's Get Started
1 Chapter 1: Let’s Get Started Chapter Overview • Welcome • Maurice Systems User Guide for Maurice, Maurice C. and Maurice S.
Page 12: Welcome
Warns you that serious physical injury can result if the listed precautions aren’t followed. Maurice Systems Maurice, Maurice C. and Maurice S. systems give you identity, purity and heterogeneity data on your biolog- ics, and get you to results faster with short development times and simple workflows! •...
Page 13: Getting Your Lab Ready
Chapter 2: Getting Your Lab Ready Chapter Overview • Introduction • Space Requirements • Physical Specifications • Electrical Requirements • Environmental Requirements • Software and Computer Requirements • General Guidelines and Information User Guide for Maurice, Maurice C. and Maurice S.
Page 14: Introduction
This chapter will help you prepare the lab for Maurice. Please have the space, electrical and environmental requirements ready prior to scheduling your installation. NOTE: Please wait for an authorized ProteinSimple Field Service Engineer to unpack and install Maurice for you. Don’t try doing this yourself. Handling Maurice incorrectly could cause injury to yourself or dam- age to the system.
Page 15: Physical Specifications
Maurice likes a consistent temperature in the lab (not too hot – not too cold). He works best when condi- tions stay within these ranges: Requirement Specification Operating temperature range 18 - 25 °C (64 - 77 °F) Operating humidity range 20-80% relative, non-condensing Table 2-2: Environmental requirements. User Guide for Maurice, Maurice C. and Maurice S.
Page 16: Software And Computer Requirements
Take all the standard precautions when lifting or moving Maurice. Since Maurice systems weigh 46 kg (100 lb), you should not lift him by yourself. Two people should lift him onto the lab bench. User Guide for Maurice, Maurice C. and Maurice S.
Page 17: Maurice
7 Chapter 3: Maurice Chapter Overview • Maurice Systems • External Components • Internal Components • Rear Panel • Computer Workstation User Guide for Maurice, Maurice C. and Maurice S.
Page 18: Maurice Systems
Maurice Systems page 8 Maurice Systems Maurice, Maurice C. and Maurice S. systems include the instrument, computer workstation, Compass for iCE software and cIEF or CE-SDS Cartridges. Maurice with Computer Workstation cIEF and CE-SDS Cartridges All systems have the same hardware components, computer and software, the only difference between them are the applications you can run: •...
Page 19: External Components
Then just touch the metal touch plate on the top of the door to open it. Close it by pushing the door until you hear the latch engage. NOTE: Maurice’ s door must be closed before starting a batch. User Guide for Maurice, Maurice C. and Maurice S.
Page 20: Status Light
The cartridge slot holds Maurice’s ready-to-go application cartridges. The cartridge it holds depends on the system: • Maurice: cIEF and CE-SDS Cartridges • Maurice C.: cIEF Cartridges only • Maurice S.: CE-SDS Cartridges only User Guide for Maurice, Maurice C. and Maurice S.
Page 21: Sample And Reagent Platform
Maurice and Maurice C. “ and Chapter 8, “Running CE-SDS Applications on Maurice and Mau- rice S. “ Sample and Reagent Platform Maurice’s sample and reagent platform has two rows for batch reagents. These reagents are kept at room temperature. User Guide for Maurice, Maurice C. and Maurice S.
Page 22 This prevents excess condensation. NOTE: Because Maurice holds the sample block temperature after a run until you open the door, samples are still viable for your next run and after overnight runs. User Guide for Maurice, Maurice C. and Maurice S.
Page 23 Column M (left): These reagents are used for on-board mixing. Only use 6 mL reagent vials in this row. OBM Reagent Positions in OBM Reagent Positions in 96-well Plate Insert 48-vial Insert User Guide for Maurice, Maurice C. and Maurice S.
Page 24: Rear Panel
Only use the power supply cord provided with Maurice. If the cord is damaged, please contact Pro- teinSimple Technical Support. !WARNING! You can't replace or service any parts on Maurice except the power entry fuse. User Guide for Maurice, Maurice C. and Maurice S.
Page 25: System Labels
NOTE: Serial numbers are used to identify individual instruments. System Labels A full system label is located on the rear panel. It includes the ProteinSimple location, system model, power requirements, serial number and certification markings. User Guide for Maurice, Maurice C. and Maurice S.
Page 26: Computer Workstation
The PC has two built-in Ethernet ports, one is used for Maurice and the other is available for your company’s network. ProteinSimple configures one port to have a fixed IP for a local link connection to the instrument, the other is configurable by users and will typically use a DHCP for dynamic IP.
Page 27 Computer Workstation page 17 User Guide for Maurice, Maurice C. and Maurice S.
Page 28: Compass For Ice Overview
• Checking for and Installing New Versions of Compass for iCE • Viewing Release Notes • Viewing the Software Log • Compass for iCE Version Information • Directory and File Information User Guide for Maurice, Maurice C. and Maurice S.
Page 29: Launching Compass For Ice
Run Summary - Check out the status of your run. • Analysis - Take a look at the data from your experiment. Each screen has these components: Title bar Screen Tabs Main Menu Instrument Status Bar Screen Panes Status Bar User Guide for Maurice, Maurice C. and Maurice S.
Page 30: Changing The Screen View
The Batch screen is used to create, view, and edit batches. You can assign samples to 96-well plate wells or vials, create and modify methods, customize your injection list and assign methods to each of your injec- tions. User Guide for Maurice, Maurice C. and Maurice S.
Page 31: Run Summary Screen
The Run Summary screen is used to monitor status of a batch in progress, the CE-SDS separation or cIEF Focus series for each injection and the current and voltage plots for each injection. User Guide for Maurice, Maurice C. and Maurice S.
Page 32 Compass for iCE Overview page 22 User Guide for Maurice, Maurice C. and Maurice S.
Page 33: Analysis Screen
The Analysis screen is used to view data from your batch, including the graph view (electropherograms) and a table with your results. You can also analyze your data for completed runs. User Guide for Maurice, Maurice C. and Maurice S.
Page 34 Compass for iCE Overview page 24 User Guide for Maurice, Maurice C. and Maurice S.
Page 35: Screen Panes
The instrument status bar is used to start batches and cleaning protocols, indicate system status and show run progress. More details on instrument control and status can be found in Chapter 10, “Controlling Maurice, Maurice C. and Maurice S. “ . User Guide for Maurice, Maurice C. and Maurice S.
Page 36: Screen Tab
You can find menus and commands available for each screen in the Chapter 5, “cIEF Batches“, Chapter 6, “CE-SDS Batches“, Chapter 9, “Run Status“, Chapter 12, “cIEF Data Analysis“ and Chapter 11, “CE-SDS Data Analysis“. User Guide for Maurice, Maurice C. and Maurice S.
Page 37: File Menu
The File menu contains basic file commands. Edit Menu The Edit menu contains basic editing commands, analysis and preferences options. Specific details on pref- erences are described in Chapter 13, “Setting Your Preferences“. User Guide for Maurice, Maurice C. and Maurice S.
Page 38: View Menu
Instrument Menu The Instrument menu is only available when the software is connected directly to your instrument. You can learn more about instrument control options in Chapter 10, “Controlling Maurice, Maurice C. and Maurice S. “ Window Menu The Window menu lets you to switch between the Batch, Run Summary or Analysis screens, and restore screens to the default layout.
Page 39: Help Menu
The Help menu gives you access to Help, software updates, release notes and other software info. • User Guide - Displays the User Guide for Maurice, Maurice C. and Maurice S. • Check for Updates - Automatically checks to see if a new version of Compass for iCE is available.
Page 40: Resizing The Screen Tab
To maximize a pane - Click the maximize button in the upper right corner or double-click the tab. The other panes in the screen will automatically minimize to pane bars in the task area along the win- dow border. User Guide for Maurice, Maurice C. and Maurice S.
Page 41: Changing The Location Of Screen Panes
Area guides with a folder let you know that if the pane is dropped at that location, it will be added as a new tab in an area with one or more pane tabs. User Guide for Maurice, Maurice C. and Maurice S.
Page 42 To move a detached pane back inside the main window - Right click the tab and deselect Detached. • To restore all panes to their original locations - Select Window in the main menu and click Default Layout. User Guide for Maurice, Maurice C. and Maurice S.
Page 43: Software Help
Compass for iCE. We recommend you review these notes whenever a software update is installed. NOTE: You can contact ProteinSimple Technical Support to request the release notes for new versions of Compass for iCE before you install it.
Page 44: Viewing The Software Log
Compass Log to view the software log file. Compass for iCE Version Information Select Help and click About Compass for iCE to view the software version and build number information. User Guide for Maurice, Maurice C. and Maurice S.
Page 45: Directory And File Information
The main Compass for iCE directory is located in the Program Files folder, and also contains PDF files of the User Guide for Maurice, Maurice C. and Maurice S. Batch and run files are located in the Documents folder in the User directory on your computer: •...
Page 46: File Types
Run Files - Use a *.mbz file extension. The default file format for run files is Date_Time_BatchName. An example run file name would be 2016-01-28_18-50-53_CE-SDS.mbz. • Analysis Settings Files - Exported analysis settings files use a *.settings file extension. User Guide for Maurice, Maurice C. and Maurice S.
Page 47 37 Chapter 4: Compass for iCE Overview User Guide for Maurice, Maurice C. and Maurice S.
Page 48: Chapter 5: Cief Batches
Making Changes to a Batch • Viewing and Editing Batches in Completed Runs • Copying and Pasting Injection Names or Sample IDs from other Documents • Importing and Exporting Methods • Batch Reports User Guide for Maurice, Maurice C. and Maurice S.
Page 49: Batch Screen Overview
History - Lists all batch file events from initial creation to the most current update. • Notes - Lets you enter specific information about your batch. • Methods - Lets you create methods and enter method parameters used in the batch. User Guide for Maurice, Maurice C. and Maurice S.
Page 50: Software Menus Active In The Batch Screen
Software Menus Active in the Batch Screen These main menu items are active in the Batch screen: • File • Edit • Instrument (when the software is connected to Maurice or Maurice S.) • Window • Help File Menu These File menu options are active: •...
Page 51 • Preferences - Lets you set and save your preferences for Access Control, data export, graph colors, grouped data and Twitter settings. See Chapter 13, “Setting Your Preferences“ for more information. User Guide for Maurice, Maurice C. and Maurice S.
Page 52: Opening A Batch
Batches folder and select a different one. 3. To make changes to the batch, see the steps in “Creating a New Batch” on page 43. When you’re done, select File from the main menu and click Save. User Guide for Maurice, Maurice C. and Maurice S.
Page 53: Creating A New Batch
Batch: NOTE: If you’re using a Maurice system, both cIEF and CE-SDS template batches are available in the menu. 2. Select Maurice cIEF. A batch using the default method will display. User Guide for Maurice, Maurice C. and Maurice S.
Page 54: Step 2 - Assign Your Samples
• M4 - 6 mL water vial 1. To assign samples, select 48 vials or a 96-well plate depending on what you’re running. Clicking on the vial/plate icon toggles between formats. User Guide for Maurice, Maurice C. and Maurice S.
Page 55 • Add samples with preassigned methods: Highlight the well or vial positions your samples are located in, then right-click and select a method. User Guide for Maurice, Maurice C. and Maurice S.
Page 56 Reinjections are enabled by default, but if you don’t want to use this feature just click the reinject icon to toggle it off. User Guide for Maurice, Maurice C. and Maurice S.
Page 57: Step 3 - Assign Your Method Parameters
To import a saved method: 1. Select File in the main menu and click Import Method. 2. Select a method file (*.method) and click OK. The imported information will display in the Method pane. User Guide for Maurice, Maurice C. and Maurice S.
Page 58 To remove a profile step: Select the row you want to remove and click Remove. 3. Click the first cell in the Detection column the selection button [...] to set your exposure times for absorption and fluorescence detection modes. User Guide for Maurice, Maurice C. and Maurice S.
Page 59 4. Click the first cell in the Sample Load(s) column and set the load time in seconds. NOTE: We recommend using the default Sample Load time of 55 seconds. Please contact ProteinSimple Technical Support if you have questions on the Sample Load time to use for your application.
Page 60 To remove a pI marker: Select the row you want to remove and click Remove. 6. Optional: Click the first cell in the Ampholytes column and enter the ampholytes you’re using. User Guide for Maurice, Maurice C. and Maurice S.
Page 61: Step 4 - Set Up Your Injections
Your Samples” are automatically added to this list. Selecting an injection in the table also selects the sample the injection will be made from in the plate or vial map in the Layout pane and vice-versa. User Guide for Maurice, Maurice C. and Maurice S.
Page 62 ID. If you’ve already changed the injection name manually, updating the sample ID will not update the injection name. The sample name also displays when you hover the mouse over the sample in the plate or vial map: User Guide for Maurice, Maurice C. and Maurice S.
Page 63 If you added your samples with pre-assigned methods and don’t need to make any changes, skip to the next step. Hovering over a method name displays the method parameters: User Guide for Maurice, Maurice C. and Maurice S.
Page 64 To copy and paste injections: Right-click on an injection in the Injection table and select Copy. Click on the injection number above where you want to insert the copied injection, right click and select Paste. The copied injection will be inserted under the row you selected. User Guide for Maurice, Maurice C. and Maurice S.
Page 65: Step 5 - Setup On-Board Mixing (Optional)
2. The on-board mixing function is disabled by default. Click the on-board mixing icon to toggle it on. All sample positions will now display the mixing icon, and the on-board mixing reagent vial positions will also display on the left: User Guide for Maurice, Maurice C. and Maurice S.
Page 66 The vial in the M1 position is used for samples 1-48, and the vial in M2 will automatically display and be used for samples 49-96: User Guide for Maurice, Maurice C. and Maurice S.
Page 67 57 Chapter 5: cIEF Batches 3. To turn on-board mixing off for specific samples, select those well(s) or vial(s), right click and select Mix- ing Off. User Guide for Maurice, Maurice C. and Maurice S.
Page 68: Step 6 - Add Programmed Pauses And Stops
Pauses are helpful when you want to review injection results before continuing with the rest of the batch. 1. Highlight the injection you want to pause at and click Pause. The batch will pause after that injection is complete. User Guide for Maurice, Maurice C. and Maurice S.
Page 69 Data collected for injections completed up to the point where the batch was stopped are saved in the .mbz file. User Guide for Maurice, Maurice C. and Maurice S.
Page 70: Step 7 - Add Batch Notes (Optional)
Step 8 - Modify Default Analysis Parameters (Optional) You can preset the parameters used to analyze data generated with the batch. We recommend using the default parameters for cIEF applications, but if you need to modify parameters: User Guide for Maurice, Maurice C. and Maurice S.
Page 71: Step 9 - Save Your Batch
Step 9 - Save Your Batch 1. Once all of your sample, method and injection info is entered, select File > Save. Enter any comments on the batch if you want, then click Save. User Guide for Maurice, Maurice C. and Maurice S.
Page 72: Viewing Replicate Injections
Injections with replicates have an arrow next to the injection number. You can expand or collapse the list of replicate injections by toggling the arrow: • To show all replicate injections in the batch, click the Expand All Injections button. User Guide for Maurice, Maurice C. and Maurice S.
Page 73: On-Board Mixing
OBM is really useful when you need to analyze proteins using platform methods and when your proteins have a limited stability in their sample solution. It also minimizes assay complexity while maximizing mixing accuracy and consistency. User Guide for Maurice, Maurice C. and Maurice S.
Page 74 The vial in the M1 position is used for samples 1-48, and the vial in M2 will automatically display and be used for samples 49-96: User Guide for Maurice, Maurice C. and Maurice S.
Page 75 65 Chapter 5: cIEF Batches To turn on-board mixing off for specific samples, select those well(s) or vial(s), right click and select Mixing Off. User Guide for Maurice, Maurice C. and Maurice S.
Page 76: Batch History
Clicking on the History pane shows the batch event history, starting with the date and time the batch was created through the most current event. Clicking on a row in the table displays the full event details in the box under the table. User Guide for Maurice, Maurice C. and Maurice S.
Page 77: Making Changes To A Batch
Edit in the main menu and select Copy. 3. Open a document and click Paste. Making Changes to a Batch 1. Select File in the main menu and click Open Batch. User Guide for Maurice, Maurice C. and Maurice S.
Page 78: Viewing And Editing Batches In Completed Runs
Update sample and method names as needed. 4. Click the Analysis screen. Then select File from the main menu and click Save Save As to save the new changes to the run file. User Guide for Maurice, Maurice C. and Maurice S.
Page 79: Copying And Pasting Injection Names Or Sample Ids
2. Select an injection in the Injection pane, then hold the Ctrl+V keys to paste. You can also select the injection, right click and select Paste. The injection names are pasted into the Injection pane: User Guide for Maurice, Maurice C. and Maurice S.
Page 80 2. Select a Sample ID in the Injection pane, then hold the Ctrl+V keys to paste. You can also select the Sample ID, right click and select Paste. User Guide for Maurice, Maurice C. and Maurice S.
Page 81: Importing And Exporting Methods
Methods in an open batch or run file can be exported as a separate file. This lets you import the same method into another batch later, rather than having to re-enter method information manually. User Guide for Maurice, Maurice C. and Maurice S.
Page 82: Importing A Method
2. In the Method pane, select the method you want to export. To export more than one method at the same time, hold the Ctrl key to select multiple methods. 3. Select File in the main menu and click Export Method. The following window displays: User Guide for Maurice, Maurice C. and Maurice S.
Page 83: Batch Reports
Batch Report. 4. The report name defaults to the run file name. If you want to change it, type in the Report Name box to make updates. User Guide for Maurice, Maurice C. and Maurice S.
Page 84 Batch created using the factory default Maurice cIEF Created By: Jacquelyn Sun 10:37 PM Jun 11, 2017 CDT Software Version: 2.0.8, Build ID: 0606 C:\Users\Jacquelyn\Documents\Compass for iCE\Runs\2016-01-21_09-46-39_mAb11_Prep20160121_QC(0).mbz Computer: DESKTOP-C7FPQGB Page 1 of 2 User Guide for Maurice, Maurice C. and Maurice S.
Page 85 Save protocol and template changes Auto-saved Created By: Jacquelyn Sun 10:37 PM Jun 11, 2017 CDT Software Version: 2.0.8, Build ID: 0606 C:\Users\Jacquelyn\Documents\Compass for iCE\Runs\2016-01-21_09-46-39_mAb11_Prep20160121_QC(0).mbz Computer: DESKTOP-C7FPQGB Page 2 of 2 User Guide for Maurice, Maurice C. and Maurice S.
Page 86: Chapter 6: Ce-Sds Batches
Making Changes to a Batch • Viewing and Editing Batches in Completed Runs • Copying and Pasting Injection Names or Sample IDs from other Documents • Importing and Exporting Methods • Batch Reports User Guide for Maurice, Maurice C. and Maurice S.
Page 87: Batch Screen Overview
Layout - Displays a map of either the 96-well plate or 48-vial tray of batch sample locations. • Injections - Lists the injections, sample ID, sample locations and methods that Maurice or Maurice S. will execute for each sample in the batch.
Page 88: Software Menus Active In The Batch Screen
Software Menus Active in the Batch Screen These main menu items are active in the Batch screen: • File • Edit • Instrument (when the software is connected to Maurice or Maurice S.) • Window • Help File Menu These File menu options are active: •...
Page 89 • Preferences - Lets you set and save your preferences for Access Control, data export, graph colors, grouped data and Twitter settings. See Chapter 13, “Setting Your Preferences“ for more information. User Guide for Maurice, Maurice C. and Maurice S.
Page 90: Opening A Batch
Batches folder and select a different one. 3. To make changes to the batch, see the steps in “Creating a New Batch” on page 81. When you’re done, select File from the main menu and click Save. User Guide for Maurice, Maurice C. and Maurice S.
Page 91: Creating A New Batch
Batch: NOTE: If you’re using a Maurice system, both cIEF and CE-SDS template batches are available in the menu. 2. Select Maurice CE-SDS. A batch using the default method will display. User Guide for Maurice, Maurice C. and Maurice S.
Page 92: Step 2 - Assign Your Samples
N4 - Running Buffer - Bottom with clear screw cap 1. To assign samples, select a 96-well plate or 48 vials depending on what you’re running. Clicking on the vial/plate icon toggles between formats. User Guide for Maurice, Maurice C. and Maurice S.
Page 93 Add samples with preassigned methods: Highlight the well or vial positions your samples are located in, then right-click and select a method. Either option of adding samples populates the Injections table: User Guide for Maurice, Maurice C. and Maurice S.
Page 94 If a sample was reinjected, the injection row will be flagged in the Injections pane in the Run Summary screen. See “Injection Flags” on page 197 for more info. A maximum of 10 reinjections are allowed per batch. User Guide for Maurice, Maurice C. and Maurice S.
Page 95: Step 3 - Assign Your Method Parameters
To create a new method or make changes to an existing one: 1. In the Methods table, click the first cell in the Name column and enter a new method name if needed. User Guide for Maurice, Maurice C. and Maurice S.
Page 96 NOTE: Run your reduced IgG samples and IgG Standard for 25 minutes and the CE-SDS MW Markers for 30 minutes. Run your non-reduced IgG samples and IgG Standard for 35 minutes. The default separation voltage for all sample types is 5750 volts. User Guide for Maurice, Maurice C. and Maurice S.
Page 97: Step 4 - Set Up Your Injections
Your Samples” are automatically added to this list. Selecting an injection in the table also selects the sample the injection will be made from in the plate or vial map in the Layout pane and vice-versa. User Guide for Maurice, Maurice C. and Maurice S.
Page 98 ID. If you’ve already changed the injection name manually, updating the sample ID will not update the injection name. The sample name also displays when you hover the mouse over the sample in the plate or vial map: User Guide for Maurice, Maurice C. and Maurice S.
Page 99 If you added your samples with pre-assigned methods and don’t need to make any changes, skip to the next step. User Guide for Maurice, Maurice C. and Maurice S.
Page 100 To copy and paste injections: Right-click on an injection in the Injection table and select Copy. Click on the injection number above where you want to insert the copied injection, right click and select Paste. The copied injection will be inserted under the row you selected. User Guide for Maurice, Maurice C. and Maurice S.
Page 101: Step 5 - Add Programmed Pauses And Stops
1. Highlight the injection you want the batch to stop at and click Stop. Maurice will continue the batch through the selected injection, then stop the batch and perform end-run cleanup steps. User Guide for Maurice, Maurice C. and Maurice S.
Page 102: Step 6 - Add Batch Notes (Optional)
Step 7 - Modify Default Analysis Parameters (Optional) You can preset the parameters used to analyze data generated with the batch. We recommend using the default parameters for CE-SDS applications, but if you need to modify parameters: User Guide for Maurice, Maurice C. and Maurice S.
Page 103: Step 8 - Save Your Batch
Step 8 - Save Your Batch 1. Once all of your sample, method and injection info is entered, select File > Save. Enter any comments on the batch if you want, then click Save. User Guide for Maurice, Maurice C. and Maurice S.
Page 104: Viewing Replicate Injections
Injections with replicates have an arrow next to the injection number. You can expand or collapse the list of replicate injections by toggling the arrow: • To show all replicate injections in the batch, click the Expand All Injections button. User Guide for Maurice, Maurice C. and Maurice S.
Page 105: Batch History
Clicking on the History pane shows the batch event history, starting with the date and time the batch was created through the most current event. Clicking on a row in the table displays the full event details in the box under the table. User Guide for Maurice, Maurice C. and Maurice S.
Page 106: Making Changes To A Batch
Edit in the main menu and select Copy. 3. Open a document and click Paste. Making Changes to a Batch 1. Select File in the main menu and click Open Batch. User Guide for Maurice, Maurice C. and Maurice S.
Page 107: Viewing And Editing Batches In Completed Runs
Update sample and method names as needed. 4. Click the Analysis screen. Then select File from the main menu and click Save Save As to save the new changes to the run file. User Guide for Maurice, Maurice C. and Maurice S.
Page 108: Copying And Pasting Injection Names Or Sample Ids
2. Select an injection in the Injection pane, then hold the Ctrl+V keys to paste. You can also select the injection, right click and select Paste. The injection names are pasted into the Injection pane: User Guide for Maurice, Maurice C. and Maurice S.
Page 109 2. Select a Sample ID in the Injection pane, then hold the Ctrl+V keys to paste. You can also select the Sample ID, right click and select Paste. User Guide for Maurice, Maurice C. and Maurice S.
Page 110: Importing And Exporting Methods
Methods in an open batch or run file can be exported as a separate file. This lets you import the same method into another batch later, rather than having to re-enter method information manually. User Guide for Maurice, Maurice C. and Maurice S.
Page 111: Importing A Method
2. In the Method pane, select the method you want to export. To export more than one method at the same time, hold the Ctrl key to select multiple methods. 3. Select File in the main menu and click Export Method. The following window displays: User Guide for Maurice, Maurice C. and Maurice S.
Page 112: Batch Reports
Batch Report. 4. The report name defaults to the run file name. If you want to change it, type in the Report Name box to make updates. User Guide for Maurice, Maurice C. and Maurice S.
Page 113 Created By: Jacquelyn Sat 8:23 PM Jun 10, 2017 CDT Software Version: 2.0.8, Build ID: 0606 C:\Users\Jacquelyn\Documents\Compass for iCE\Runs\MW ladder assigned_MW Ladder Sol Test IS final QC 110 ms Maurice CE-SDS.MBZ Computer: DESKTOP-C7FPQGB Page 1 of 4 User Guide for Maurice, Maurice C. and Maurice S.
Page 114 Created By: Jacquelyn Sat 8:23 PM Jun 10, 2017 CDT Software Version: 2.0.8, Build ID: 0606 C:\Users\Jacquelyn\Documents\Compass for iCE\Runs\MW ladder assigned_MW Ladder Sol Test IS final QC 110 ms Maurice CE-SDS.MBZ Computer: DESKTOP-C7FPQGB Page 4 of 4 User Guide for Maurice, Maurice C. and Maurice S.
Page 115 105 Chapter 6: CE-SDS Batches User Guide for Maurice, Maurice C. and Maurice S.
Page 116: Running Cief Applications On
Running cIEF Applications on Maurice and Maurice C. Chapter Overview • Before You Throw the Switch • Power Up • Running cIEF Applications • Editing a Running Batch • Checking Your Data User Guide for Maurice, Maurice C. and Maurice S.
Page 117: Before You Throw The Switch
1% Methyl Cellulose Solution • iCE Electrolyte Kit • Deionized (DI) water • Glass reagent vials, 2 mL • 96-well plate or vials with integrated inserts for samples • Clear screw caps for vials User Guide for Maurice, Maurice C. and Maurice S.
Page 118: Step 1: Prep Your Markers, Samples And Reagents
Transfer this solution to a 1.5 mL microcentrifuge tube. 6. Vortex the tube 3 times, 5 seconds each. 7. Centrifuge the tube at 10,000 xg for 3 minutes to sediment any particulates. User Guide for Maurice, Maurice C. and Maurice S.
Page 119 96-well plate by inserting the pipette tip all the way to the bottom to avoid introducing bub- bles. Note: Make sure to check for and remove any bubbles at the bottom of the sample vial or well. User Guide for Maurice, Maurice C. and Maurice S.
Page 120 DI water, rinse caps thoroughly with DI water again and air dry them before reusing. 1. Pipette 2 mL of 0.5% Methyl Cellulose into a glass reagent vial, label and close with a blue pressure cap. User Guide for Maurice, Maurice C. and Maurice S.
Page 121 If you are running 48 samples or less, add 6 mL of IEF Separation Mix to one 6 mL glass reagent vial. For more than 48 samples, prepare two vials. d. Add 6 mL of DI water to two 6 mL glass reagent vials. User Guide for Maurice, Maurice C. and Maurice S.
Page 122: Step 2: Prep The Cartridge
NOTE: When you remove the cartridge from its packaging, make sure the cartridge inlet doesn’t come in contact with any surface. 2. Put the cartridge on a flat surface with its electrolyte tanks facing up. 3. Remove the stoppers from both electrolyte tanks. User Guide for Maurice, Maurice C. and Maurice S.
Page 123 6. Seal each tank with the rubber stoppers. Use the grey stopper for the OH tank and the red one for the tank. If excess liquid comes out of the tank, make sure to wipe it with a laboratory wipe. User Guide for Maurice, Maurice C. and Maurice S.
Page 124: Step 3: Install The Cartridge
4. Lift the cartridge and hold it vertically using the finger holds on either side, cartridge inlet down, with the cIEF label facing you. 5. Gently insert it into the slot. You’ll feel the alignment groove on the cartridge align inside the slot when you insert it. User Guide for Maurice, Maurice C. and Maurice S.
Page 125: Step 4: Load Samples And Reagents
The two reagent rows in Maurice’ s reagent and sample platform are kept at room temperature. Pressure caps are blue and have a raised, ringed surface. They should only be used in Reagent Row P. Use reagent vials with clear screw caps in Row N. User Guide for Maurice, Maurice C. and Maurice S.
Page 126 M4 - 6 mL water vial Depending on what you prepared your samples in, put your 96-well sample plate in the metal plate insert or OBM Reagents M1-M4 your sample vials in the metal vial insert. User Guide for Maurice, Maurice C. and Maurice S.
Page 127: Step 5: Create A Batch
3. Close the instrument door. Maurice locks it automatically. Step 5: Create a Batch 1. Launch Compass for iCE. 2. Select the Batch tab. This is where you’ll enter sample/injection information, methods and batch parameters. User Guide for Maurice, Maurice C. and Maurice S.
Page 128 To use an existing batch: In the main menu, select File > Open Batch. NOTE: If you’re making changes to an existing batch, follow the steps in this section. Otherwise skip to “Step 6: Start the Batch” on page 131. User Guide for Maurice, Maurice C. and Maurice S.
Page 129 4. In the Layout pane, clicking on the vial/plate icon toggles between formats. Select 48 vials or a 96-well plate depending on what you’re running. 5. Use your mouse to highlight the well positions for each of your samples, then click Add. This populates the Injections table: User Guide for Maurice, Maurice C. and Maurice S.
Page 130 NOTE: The two reagent rows are kept at room temperature. 8. Enter your method parameters in the Methods pane. User Guide for Maurice, Maurice C. and Maurice S.
Page 131 (in minutes) and voltage. c. Click the first cell in the Detection column then click the selection button [...] to set your exposure times for absorption and fluorescence detection modes. User Guide for Maurice, Maurice C. and Maurice S.
Page 132 Click the first cell in the Sample Load(s) column and set the load time in seconds. e. Click the first cell in the pI Markers column to select pI markers. Add new markers or remove existing ones then click OK. User Guide for Maurice, Maurice C. and Maurice S.
Page 133 If you change the sample ID when the default injection name is still being used, the injection name will automatically update to match the sample ID. If you’ve already changed the injection name manually, updating the sample ID will not update the injection name. User Guide for Maurice, Maurice C. and Maurice S.
Page 134 • To add sequential replicate injections: Highlight an injection and click Replicate. A new injec- tion will be added under the row you selected User Guide for Maurice, Maurice C. and Maurice S.
Page 135 • M3 - 6 mL water vial • M4 - 6 mL water vial b. The on-board mixing function is disabled by default. Click the on-board mixing icon to toggle it on. User Guide for Maurice, Maurice C. and Maurice S.
Page 136 The vial in the M1 position is used for samples 1-48, and the vial in M2 will automatically display and be used for samples 49-96: User Guide for Maurice, Maurice C. and Maurice S.
Page 137 Running cIEF Applications page 127 c. To turn on-board mixing off for specific samples, select those well(s) or vial(s), right click and select Mixing Off. User Guide for Maurice, Maurice C. and Maurice S.
Page 138 To program a pause: Highlight the injection you want to pause at and click Pause. The batch will pause after that injection is complete. To resume the batch, click Continue in the instrument status bar. User Guide for Maurice, Maurice C. and Maurice S.
Page 139 Select Edit from the main menu and click Default Analysis. The following screen will display: User Guide for Maurice, Maurice C. and Maurice S.
Page 140 15. Once all of your sample, method and injection info is entered, select File > Save. Enter any comments on the batch if you want, then click Save. 16. Enter a name for your batch then click Save. User Guide for Maurice, Maurice C. and Maurice S.
Page 141: Step 6: Start The Batch
6. Enter any run details you’d like in the Comments box (optional). 7. Click Start to start the run. NOTE: The indicator light on Maurice’ s front panel will pulse slowly while he’ s running the batch. User Guide for Maurice, Maurice C. and Maurice S.
Page 142 To view results, analyze results or change analysis parameters on completed injections while the batch is still running, click the Analysis tab. See Chapter 12, “cIEF Data Analysis“ for more details. User Guide for Maurice, Maurice C. and Maurice S.
Page 143: Editing A Running Batch
“Setting Up Maurice Systems to Send Tweets” on page 440 for more info. Editing a Running Batch You can pause a batch after it's started to make updates or add samples and reagents. 1. Select Instrument > Pause or select the Pause button. User Guide for Maurice, Maurice C. and Maurice S.
Page 144 3. When Maurice completes the current injection, his status will change to Paused and the progress bar will turn grey. NOTE: Maurice’ s status light will change from a pulsing blue to a blue pulse with magenta flashes which means he’ s paused the run. User Guide for Maurice, Maurice C. and Maurice S.
Page 145: Post-Batch Procedures
2. Remove your reagent vials and samples and discard. 3. Gently pull out the cartridge making sure the cartridge inlet doesn’t come into contact with any surface. User Guide for Maurice, Maurice C. and Maurice S.
Page 146 Fill each tank with 2 mL DI water, then aspirate it out. Repeat this rinse 3 times. NOTE: Make sure not to get any liquid on the cartridge’ s optical window. User Guide for Maurice, Maurice C. and Maurice S.
Page 147 Depending on the samples used, the cartridges, plates and vials may constitute a chemi- cal or a biohazard. Dispose of the cartridges, plates and vials in accordance with good lab- User Guide for Maurice, Maurice C. and Maurice S.
Page 148: Checking Your Data
Detection page. Step 2: Check Your pI Markers To make sure your pI markers are identified correctly: 1. Go to the Analysis screen. 2. Click Markers in the View bar. User Guide for Maurice, Maurice C. and Maurice S.
Page 149 To set an unidentified peak as a pI marker: Right-click the peak in the electropherogram or Peaks table and select Force Marker. Compass will assign that peak as a pI marker, and correctly reassign the remaining pI marker peaks. User Guide for Maurice, Maurice C. and Maurice S.
Page 150 A lock icon indicating the pI marker was set manually will display next to the peak in the Peaks table and a check mark will appear next to the injection in the Experiment pane to show a manual correction was made. User Guide for Maurice, Maurice C. and Maurice S.
Page 151: Step 3: Checking Sample Peaks
7. Repeat the previous steps for the remaining pI marker peaks as needed in the current injection and for all other injections to make sure all your pI markers are identified correctly. Step 3: Checking Sample Peaks All detected peaks will be labeled automatically with the calculated protein pI. User Guide for Maurice, Maurice C. and Maurice S.
Page 152 Peaks table and select Remove Peak. Compass will no longer identify it as a sample peak in the electro- pherogram and the peak data will be removed in the results table. User Guide for Maurice, Maurice C. and Maurice S.
Page 153 To identify a peak as a sample peak: Right-click the peak in the electropherogram or Peaks table and select Peak. Compass will calculate and display the results for the peak in the results table and identify the peak in the electropherogram. User Guide for Maurice, Maurice C. and Maurice S.
Page 154: Step 4: Assigning Peak Names
Step 4: Assigning Peak Names Compass can also optionally identify and automatically name sample peaks using user-specified peak name settings. For more information on how to do this, see “Manual Peak Integration” on page 406. User Guide for Maurice, Maurice C. and Maurice S.
Page 155: Running Ce-Sds Applications On Maurice And Maurice S
Chapter 8: Running CE-SDS Applications on Maurice and Maurice S. Chapter Overview • Before You Throw the Switch • Power Up • Running CE-SDS Applications • Post-batch Procedures • Checking Your Data User Guide for Maurice, Maurice C. and Maurice S.
Page 156: Before You Throw The Switch
• 25X Internal Standard • Glass reagent vials, 2 mL • 96-well plates • Clear screw caps for vials • Orange pressure caps for vials • Maurice CE-SDS IgG Standard (optional) User Guide for Maurice, Maurice C. and Maurice S.
Page 157: Step 1: Prep Your Internal Standard, Samples And Reagents
2. Reconstitute by adding 240 μL of 1X Sample Buffer. Pipette up and down a few times to mix thoroughly. This results in a 25X Internal Standard solution. NOTE: Don’t vortex the reconstituted Internal Standard during prep. User Guide for Maurice, Maurice C. and Maurice S.
Page 158 Transfer the solution to a microcentrifuge tube. 5. Add 2 μL of reconstituted 25X Internal Standard. 6. Add 2.5 μL of 14.2 M β-mercaptoethanol. 7. Mix thoroughly by vortex. User Guide for Maurice, Maurice C. and Maurice S.
Page 159 2. Close the vials with a clear screw cap. 3. Place the vials in a centrifuge using a vial adapter (12 mm, 2 mL vials) and spin for 10 minutes at 1000 xg. User Guide for Maurice, Maurice C. and Maurice S.
Page 160 4. Mix thoroughly. 5. Centrifuge the tube and heat the mixture in a water bath or thermocycler at 70 °C for 10 minutes. 6. Put the tube on ice for 5 minutes. User Guide for Maurice, Maurice C. and Maurice S.
Page 161 If you’re using vials: 1. Transfer 50 μL of your samples and IgG Standard to their designated sample vials with integrated inserts. 2. Close the vials with a clear screw cap. User Guide for Maurice, Maurice C. and Maurice S.
Page 162 3. Pipette 1.0 mL of Wash Solution into a glass reagent vial, label each and close with an orange pressure cap. 4. Pipette 1.5 mL of Wash Solution into two glass reagent vials. Label each and close both with clear screw caps. User Guide for Maurice, Maurice C. and Maurice S.
Page 163: Step 2: Prep The Cartridge
1. Take the CE-SDS Cartridge out of its packaging. Save the packaging, you’ll need it later to store the car- tridge. NOTE: When you remove the cartridge from its packaging, make sure the cartridge inlet doesn’t come in contact with any surface. User Guide for Maurice, Maurice C. and Maurice S.
Page 164 The Top Running Buffer vial has metal pins on either side, so no specific orientation is necessary. Once you’ve inserted the Top Running Buffer vial, the cartridge insert and the cartridge must be kept in an upright position at all times. User Guide for Maurice, Maurice C. and Maurice S.
Page 165: Step 3: Install The Cartridge
Running CE-SDS Applications page 155 4. Slide the cartridge insert back into the cartridge. Step 3: Install the Cartridge 1. Open Maurice’s door by touching the metal plate on top of the door. User Guide for Maurice, Maurice C. and Maurice S.
Page 166 3. Lift the cartridge and hold it vertically using the finger holds on either side, cartridge inlet down, with the CE-SDS label facing you. 4. Gently insert it into the slot. You’ll feel the alignment groove on the cartridge align inside the slot when you insert it. User Guide for Maurice, Maurice C. and Maurice S.
Page 167: Step 4: Load Samples And Reagents
The two reagent rows in Maurice’ s reagent and sample platform are kept at room temperature. Pressure caps are orange and have a raised, ringed surface. They should only be used in Reagent Row P. Use reagent vials with clear screw caps in Row N. User Guide for Maurice, Maurice C. and Maurice S.
Page 168 NOTE: Well A1 on the 96-well plate should be in the top left corner of the insert. 3. If you are using a vial tray, place the condensation lid on top of the vials. 4. Close the instrument door. Maurice locks it automatically. User Guide for Maurice, Maurice C. and Maurice S.
Page 169: Step 5: Create A Batch
On Maurice systems - in the main menu, select File > New Batch > Maurice CE-SDS. • On Maurice S. systems - in the main menu, select File > New Batch. To use an existing batch: In the main menu, select File >...
Page 170 4. In the Layout pane, clicking on the vial/plate icon toggles between formats. Select a 96-well plate or 48 vials depending on what you’re running. 5. Use your mouse to highlight the well positions for each of your samples, then click Add. User Guide for Maurice, Maurice C. and Maurice S.
Page 171 If a sample was reinjected, the injection row will be flagged in the Injections pane in the Run Summary screen. See “Injection Flags” on page 197 for more info. A maximum of 10 reinjections are allowed per batch. User Guide for Maurice, Maurice C. and Maurice S.
Page 172 Select a method file (*.method) and click OK. The imported information will display in the Method pane. To create a new method or make changes to an existing one: a. Click the first cell in the Name column and enter a new method name if needed. User Guide for Maurice, Maurice C. and Maurice S.
Page 173 NOTE: Run your reduced IgG samples and IgG Standard for 25 minutes and the CE-SDS MW Markers for 30 minutes. Run your non-reduced IgG samples and IgG Standard for 35 minutes. The default separation voltage for all sample types is 5750 volts. User Guide for Maurice, Maurice C. and Maurice S.
Page 174 If you change the sample ID when the default injection name is still being used, the injection name will automatically update to match the sample ID. If you’ve already changed the injection name manually, updating the sample ID will not update the injection name. User Guide for Maurice, Maurice C. and Maurice S.
Page 175 • To add sequential replicate injections: Highlight an injection and click Replicate. A new injec- tion will be added under the row you selected User Guide for Maurice, Maurice C. and Maurice S.
Page 176 To program a pause: Highlight the injection you want to pause at and click Pause. The batch will pause after that injection is complete. To resume the batch, click Continue in the instrument status bar. User Guide for Maurice, Maurice C. and Maurice S.
Page 177 CE-SDS applications, but if you want to modify parameters: a. Select Edit from the main menu and click Default Analysis. The following screen will display: User Guide for Maurice, Maurice C. and Maurice S.
Page 178 14. Once all of your sample, method and injection info is entered, select File > Save. Enter any comments on the batch if you want, then click Save. 15. Enter a name for your batch then click Save. User Guide for Maurice, Maurice C. and Maurice S.
Page 179: Step 6: Start The Batch
6. Enter any run details you’d like in the Comments box (optional). 7. Click Start to start the run. NOTE: The indicator light on Maurice’ s front panel will pulse slowly while he’ s running the batch. User Guide for Maurice, Maurice C. and Maurice S.
Page 180 To view results, analyze results or change analysis parameters on completed injections while the batch is still running, click the Analysis tab. See Chapter 11, “CE-SDS Data Analysis“ for more details. User Guide for Maurice, Maurice C. and Maurice S.
Page 181: Editing A Running Batch
“Setting Up Maurice Systems to Send Tweets” on page 440 for more info. Editing a Running Batch You can pause a batch after it's started to make updates or add samples and reagents. 1. Select Instrument > Pause or select the Pause button. User Guide for Maurice, Maurice C. and Maurice S.
Page 182 • Add new samples • Add on-board mixing reagents if the paused batch was set up to do on-board mixing and your Maurice has this option. User Guide for Maurice, Maurice C. and Maurice S.
Page 183: Post-Batch Procedures
NOTE: If you see any separation matrix sticking to the capillary inlet, soak it in DI water for 5 minutes. Then wipe it using a lint-free laboratory wipe that's been moistened with DI water. User Guide for Maurice, Maurice C. and Maurice S.
Page 184 To dispose of a finished cartridge, put it in its original packing and discard it along with the cartridge insert and the Top Running Buffer vial per your institution's safety and waste disposal guidelines. Discard the cleaning vial you've used with that cartridge too. User Guide for Maurice, Maurice C. and Maurice S.
Page 185 Insert the cartridge in Maurice. e. In the Compass main menu, select Instrument and click Cartridge Cleanup. f. You’ll get the following message. Click OK. It’ll only take six minutes. User Guide for Maurice, Maurice C. and Maurice S.
Page 186 Read and understand the Safety Data Sheets (SDSs) provided by the manu- facturers of the chemicals in the waste content before you store, handle, or dispose of chemical waste. User Guide for Maurice, Maurice C. and Maurice S.
Page 187: Checking Your Data
5. Check that your Internal Standard in the electropherogram has been correctly identified. It’ll be labeled IS and will have a green vertical line running through it. The Internal Standard is also identified with an in the Peaks table. User Guide for Maurice, Maurice C. and Maurice S.
Page 188 To set an unidentified peak as the Internal Standard: Right-click the peak in the electropherogram or Peaks table and select Force Standard. Compass will assign that peak as the Internal Standard. User Guide for Maurice, Maurice C. and Maurice S.
Page 189 A lock icon indicating the Internal Standard was set manually will display next to the peak in the Peaks table and a check mark will appear next to the injection in the Experiment pane to show a manual cor- rection was made. User Guide for Maurice, Maurice C. and Maurice S.
Page 190 Peaks table and a check mark will appear next to the injection in the Experiment pane to show a manual correction was made. User Guide for Maurice, Maurice C. and Maurice S.
Page 191: Step 2: Set Your Molecular Weight (Mw)
Analysis. In the Analysis window, select Markers in the left sidebar. Then click the Markers Injection drop down menu to select the injection you ran your CE-SDS MW Markers in. User Guide for Maurice, Maurice C. and Maurice S.
Page 192 If you’d like to use these values, skip to the next step. If you’re using different markers, click in the MW and RMT cells to type new values, click a row and select Remove delete, or click to add a new one. User Guide for Maurice, Maurice C. and Maurice S.
Page 193 To set an unidentified peak as a MW Marker: Right-click the peak in the electropherogram or Peaks table and select Peak. Compass will assign that peak as a MW Marker, and correctly reassign the remaining marker peaks. User Guide for Maurice, Maurice C. and Maurice S.
Page 194 If an incorrect peak is identified as a MW Marker: Right-click the peak in the electropherogram or Peaks table and select Remove Peak. Compass should correctly reassign the remaining peaks as mark- ers and update the Peaks table. User Guide for Maurice, Maurice C. and Maurice S.
Page 195: Step 3: Checking Sample Peaks
All detected peaks will be labeled automatically with the RMT (default) or apparent MW (if the CE-SDS MW Markers were run). To make sure your sample proteins are identified correctly: 1. Click Samples in the View bar. 2. Click the View Selected icon in the View bar. User Guide for Maurice, Maurice C. and Maurice S.
Page 196 A check mark will appear next to the injection in the Experiment pane to indicate a manual correction was made. User Guide for Maurice, Maurice C. and Maurice S.
Page 197 Peak. Compass will calculate and display the results for the peak in the results table and identify the peak in the electropherogram. A check mark will appear next to the injection in the Experiment pane to indicate a manual correction was made. User Guide for Maurice, Maurice C. and Maurice S.
Page 198: Step 4: Assigning Peak Names
Step 4: Assigning Peak Names Compass can also optionally identify and automatically name sample peaks using user-specified peak name settings. For more information on how to do this, see “Manual Peak Integration” on page 406. User Guide for Maurice, Maurice C. and Maurice S.
Page 199: Chapter 9: Run Status
• Viewing the Separation (CE-SDS Only) • Current and Voltage Plots • Run History • Viewing Run Errors • Injection Reports • Switching Between Open Run Files • Closing Run Files User Guide for Maurice, Maurice C. and Maurice S.
Page 200: Run Summary Screen Overview
Focus Series (cIEF only) - Lets you view the recorded focusing of proteins along the pH gradient in the capillary for each injection. • IV Plot - Lets you view plots of the total current and voltage measured during the separation for each injection. User Guide for Maurice, Maurice C. and Maurice S.
Page 201 Run Summary Screen Overview page 191 User Guide for Maurice, Maurice C. and Maurice S.
Page 202: Software Menus Active In The Run Summary
Software Menus Active in the Run Summary Screen These main menu items are active in the Run Summary screen: • File • Edit • Instrument (when the software is connected to an instrument) • Window • Help User Guide for Maurice, Maurice C. and Maurice S.
Page 203 • Preferences - Lets you set and save your preferences for Access Control, data export, graph colors, grouped data and Twitter settings. See Chapter 13, “Setting Your Preferences“ for more information. User Guide for Maurice, Maurice C. and Maurice S.
Page 204: Opening Run Files
Open Run. 2. A list of the last 10 runs opened will display. Select one of these runs or click Browse to open the Runs folder and select a different file. User Guide for Maurice, Maurice C. and Maurice S.
Page 205: Batch Injection Information
Runs folder and select a different file. 5. Repeat the last two steps to open additional runs. Batch Injection Information The Injections pane lists the system protocols (Setup and Cleanup) and injections performed during the run. User Guide for Maurice, Maurice C. and Maurice S.
Page 206 Hovering your mouse over the progress bar tells you the time left for the injection. • Completed for Setup, Conditioning and Cleanup protocols and injections that are done. User Guide for Maurice, Maurice C. and Maurice S.
Page 207: Injection Flags
Run file name and path (directory location) • Batch name and type • Instrument and serial number • Type of sample tray used • Run start/complete date and time • Type of cartridge • Cartridge serial number User Guide for Maurice, Maurice C. and Maurice S.
Page 208 Path. The path can then be cop- ied into documents. The path can also be copied into the Windows Explorer address bar to launch Compass for iCE and open the run file automatically. User Guide for Maurice, Maurice C. and Maurice S.
Page 209: Viewing The Focus Series (Cief Only)
NOTE: The Focus Series plot displays in absorbance only, even if the fluorescence detection mode is selected in the Analysis settings. 1. Select an injection in the Injections pane. 2. Click the Focus Series pane. It’ll display the final focusing plot: User Guide for Maurice, Maurice C. and Maurice S.
Page 210: Viewing The Separation (Ce-Sds Only)
Viewing the Separation (CE-SDS Only) You can view your protein separation in the capillary for each injection in the Separation pane. User Guide for Maurice, Maurice C. and Maurice S.
Page 211: Current And Voltage Plots
Current and Voltage Plots To view plots of the total current and voltage measured during an injection: 1. Select an injection in the Injections pane. User Guide for Maurice, Maurice C. and Maurice S.
Page 212 NOTE: IV Plots for a run in progress won’t be available until the injection is executing. Once it starts, the plot displays in real time. User Guide for Maurice, Maurice C. and Maurice S.
Page 213: Run History
Viewing Multiple Events Items in the table with multiple analysis events have an arrow next to the date and time. You can view or hide these details by toggling the arrow: User Guide for Maurice, Maurice C. and Maurice S.
Page 214 204 Chapter 9: Run Status • To view details for all items with multiple analysis events in the run, click the Expand All button. User Guide for Maurice, Maurice C. and Maurice S.
Page 215: Copying History Info
Copy. 3. Open a document and click Paste. Viewing Run Errors If an error is detected during the run it will display in the Status pane: User Guide for Maurice, Maurice C. and Maurice S.
Page 216: Injection Reports
The report name defaults to the run file name. If you want to change it, type in the Report Name box to make updates. f. Click OK. User Guide for Maurice, Maurice C. and Maurice S.
Page 217 5. The Injection Report PDF(s) are exported to the Runs folder in the Compass for iCE directory. They’ll be in a folder with the report name used in the prior step. When the reports are done, the folder opens for you automatically. User Guide for Maurice, Maurice C. and Maurice S.
Page 218 Started run: 3170522428_Retest_2017-06-07_17-29-18_KF1077_FreshQC_Maurice CE-SDS 3170522428 Batch: KF1077_FreshQC_Maurice CE-SDS.batch Created By: Jacquelyn Wed 12:55 PM Jun 14, 2017 CDT Software Version: 2.0.8, Build ID: 0606 C:\Users\Jacquelyn\Documents\Compass for iCE\Maurice CE-SDS.mbz Computer: DESKTOP-C7FPQGB Page 1 of 1 User Guide for Maurice, Maurice C. and Maurice S.
Page 219 1139.6 61.1 16.5 745.3 1.88 Software Version: 2.0.8, Build ID: 0606 Created By: Jacquelyn Wed 12:55 PM Jun 14, 2017 CDT C:\Users\Jacquelyn\Documents\Compass for iCE\Maurice CE-SDS.mbz Computer: DESKTOP-C7FPQGB Page 1 of 2 User Guide for Maurice, Maurice C. and Maurice S.
Page 220 Injections Remaining 196 (96 guaranteed) Software Version: 2.0.8, Build ID: 0606 Created By: Jacquelyn Wed 12:55 PM Jun 14, 2017 CDT C:\Users\Jacquelyn\Documents\Compass for iCE\Maurice CE-SDS.mbz Computer: DESKTOP-C7FPQGB Page 2 of 2 User Guide for Maurice, Maurice C. and Maurice S.
Page 221: Example Analysis And Injection Report: Cief
Saved as Maurice cIEF from Compass for iCE v2.0.8-0606 Software Version: 2.0.8, Build ID: 0606 Created By: Jacquelyn Wed 12:59 PM Jun 14, 2017 CDT C:\Users\Jacquelyn\Documents\Compass for iCE\Maurice cIEF.mbz Computer: DESKTOP-C7FPQGB Page 1 of 1 User Guide for Maurice, Maurice C. and Maurice S.
Page 222 10.170 173.0 1537 0.0408 580.6 3.16 Created By: Jacquelyn Wed 12:59 PM Jun 14, 2017 CDT Software Version: 2.0.8, Build ID: 0606 C:\Users\Jacquelyn\Documents\Compass for iCE\Maurice cIEF.mbz Computer: DESKTOP-C7FPQGB Page 1 of 2 User Guide for Maurice, Maurice C. and Maurice S.
Page 223: Switching Between Open Run Files
C:\Users\Jacquelyn\Documents\Compass for iCE\Maurice cIEF.mbz Computer: DESKTOP-C7FPQGB Page 2 of 2 Switching Between Open Run Files If you’ve got more than one run file open, you can switch between viewing the run information in each. User Guide for Maurice, Maurice C. and Maurice S.
Page 224: Closing Run Files
To close the run file being viewed - Select File from the main menu and click Close. • To close all open run files - Select File from the main menu and click Close All. User Guide for Maurice, Maurice C. and Maurice S.
Page 225: Controlling Maurice, Maurice C. And Maurice S
Stopping a Run • Status Modes • Instrument Software (Embedded) Updates • Self Test • Viewing and Changing System Properties • Checking Cartridge Status • Instrument Reports • Viewing Log Files User Guide for Maurice, Maurice C. and Maurice S.
Page 226: Instrument Control
Controlling Maurice, Maurice C. and Maurice S. Instrument Control The Instrument menu lets you to control Maurice, Maurice C. and Maurice S. NOTE: Instrument menu options are only active when you’ve got a computer with Compass for iCE soft- ware connected directly to your Maurice system.
Page 227 This runs the Cleanup step that normally happens at the end of a batch. It flushes the cartridge of any reagents and samples so it’s ready to go for the next run. 1. In the Batch screen, select File > Open Batch and click Browse. User Guide for Maurice, Maurice C. and Maurice S.
Page 228 • cIEF Cartridges: If you've still got injections left and the cartridge will be used again within 24 hours, you don't need to do anything. Just leave the cartridge in Maurice. User Guide for Maurice, Maurice C. and Maurice S.
Page 229: Stopping A Run
If you won’t be starting a new run: • cIEF Cartridges: Follow the steps in “Editing a Running Batch” on page 133. • CE-SDS Cartridges: Follow the steps in “Post-batch Procedures” on page 173. User Guide for Maurice, Maurice C. and Maurice S.
Page 230: Emergency Stop
• Cleaning/Stop button - The instrument is running a cleaning protocol. The time the cleaning proto- col started and when it will be done show in the run progress bar. User Guide for Maurice, Maurice C. and Maurice S.
Page 231: Shutdown
2. The following screen displays. Click Update. If you’re not on the network: 1. Call ProteinSimple Technical Support or your FAS for assistance on getting the latest update. 2. Copy the new embedded software file onto Maurice’s computer. 3. Select Instrument >...
Page 232: Self Test
4. Browse to the location of the embedded software file, select it and click OK. 5. The following screen displays. Click Update. Self Test Maurice, Maurice C. and Maurice S. can run a series of self tests for you to make sure they’re operating prop- erly. 1. To start the test, select Instrument >...
Page 233: Viewing And Changing System Properties
Network name and address • Date and time of the instrument clock • Adapter block currently in use • Number of hours on the Deuterium (UV) lamp • Current sample chiller temperature User Guide for Maurice, Maurice C. and Maurice S.
Page 234: Checking Cartridge Status
If you’ve got a cartridge installed in the system, you can see its serial number, the injections and batches it still has available, and a history of batches and injections its run to date. To view this info, select Instrument > Properties. User Guide for Maurice, Maurice C. and Maurice S.
Page 235: Instrument Reports
When Compass for iCE is connected to a Maurice system, you can generate an instrument report that includes instrument and cartridge info, self test records and details and command logs. 1. To run the report select Instrument > Properties. 2. Click Report. The Instrument Report window displays: User Guide for Maurice, Maurice C. and Maurice S.
Page 236 3. Select the information you want included in the report. You can change the location where the report saves by clicking Browse. 4. Click OK. The Instrument Report PDF is exported to the folder specified. Once the report is done, the folder opens for you automatically. User Guide for Maurice, Maurice C. and Maurice S.
Page 237: Example Instrument Report
2017-05-24 16:09 PASSED Self Test - All Tests Created By: admin Wed 9:25 AM Jun 14, 2017 PDT Software Version: 2.0.9, Build ID: 0613 Kifer kf1077 Computer: KF1077 Page 1 of 5 User Guide for Maurice, Maurice C. and Maurice S.
Page 238 Tray Jog 10:28:48 PASSED Tray Home 10:28:46 PASSED Created By: admin Wed 9:25 AM Jun 14, 2017 PDT Software Version: 2.0.9, Build ID: 0613 Kifer kf1077 Computer: KF1077 Page 2 of 5 User Guide for Maurice, Maurice C. and Maurice S.
Page 239 11:14:26 PASSED Temp Sensor Variance 11:14:25 PASSED Created By: admin Wed 9:25 AM Jun 14, 2017 PDT Software Version: 2.0.9, Build ID: 0613 Kifer kf1077 Computer: KF1077 Page 3 of 5 User Guide for Maurice, Maurice C. and Maurice S.
Page 240 Pressure Vent 16:02:15 PASSED Pressure Leak 16:00:54 PASSED Created By: admin Wed 9:25 AM Jun 14, 2017 PDT Software Version: 2.0.9, Build ID: 0613 Kifer kf1077 Computer: KF1077 Page 4 of 5 User Guide for Maurice, Maurice C. and Maurice S.
Page 241: Viewing Log Files
Software Version: 2.0.9, Build ID: 0613 Kifer kf1077 Computer: KF1077 Page 5 of 5 Viewing Log Files Runs Log To see a history of all runs your system has performed, select Instrument > Runs: User Guide for Maurice, Maurice C. and Maurice S.
Page 242: Instrument Log
To delete a run file: Select a run file from the list and click Delete. The run file will be deleted from the history and from the Run file in the Compass for iCE directory. Instrument Log 1. Select Instrument > Properties. 2. Click Logs. The Instrument Logs window displays: User Guide for Maurice, Maurice C. and Maurice S.
Page 243 Viewing Log Files page 233 3. Click Instrument Log. User Guide for Maurice, Maurice C. and Maurice S.
Page 244 234 Chapter 10: Controlling Maurice, Maurice C. and Maurice S. 4. Click Save File As to save a copy of the log file. User Guide for Maurice, Maurice C. and Maurice S.
Page 245: Self Test Logs
Viewing Log Files page 235 Self Test Logs 1. Select Instrument > Properties. 2. Click Logs. A list of dates each self test was run displays: User Guide for Maurice, Maurice C. and Maurice S.
Page 246 3. Select a test date in the list and click View to see the individual test results: 4. Click Save File As to save a copy of the test log file. User Guide for Maurice, Maurice C. and Maurice S.
Page 247: Command Log
237 Command Log 1. Select Instrument > Properties to display your system’s properties. 2. Click Logs. The Instrument Logs window displays: 3. Click Command Log. A list of system commands displays: User Guide for Maurice, Maurice C. and Maurice S.
Page 248 238 Chapter 10: Controlling Maurice, Maurice C. and Maurice S. User Guide for Maurice, Maurice C. and Maurice S.
Page 249: Chapter 11: Ce-Sds Data Analysis
Analysis Settings Overview • Advanced Analysis Settings • Markers Analysis Settings • Peak Fit Analysis Settings • Manual Peak Integration • Peak Names Settings • Injection Reports • Importing and Exporting Analysis Settings User Guide for Maurice, Maurice C. and Maurice S.
Page 250: Analysis Screen Overview
Peaks - Shows the tabulated results for sample proteins, internal standards and CE-SDS MW Markers. • Injections - Displays a list of the sample proteins Compass for iCE names automatically using the user-defined peak name analysis parameters. User Guide for Maurice, Maurice C. and Maurice S.
Page 251: Software Menus Active In The Analysis Screen
These main menu items are active in the Analysis Screen: • File • Edit • View • Instrument (when Compass for iCE is connected to Maurice, Maurice C. or Maurice S.) • Window • Help File Menu These File menu options are active: •...
Page 252 Grouping - Displays data for injection groups. • View Region - Lets you change the x-axis range of the data displayed. • Show Hidden- Shows injections that are hidden from the data view. User Guide for Maurice, Maurice C. and Maurice S.
Page 253: Opening Run Files
Open Run. 2. A list of the last 10 runs opened will display. Select one of these runs or click Browse to open the Runs folder and select a different file. User Guide for Maurice, Maurice C. and Maurice S.
Page 254 When a run is added, its data appends to the open run file and displays as a second set of injections in all screen panes. The second run file name also appears in the title bar: 5. Repeat the last two steps to add additional runs. User Guide for Maurice, Maurice C. and Maurice S.
Page 255: How Run Data Is Displayed
NOTE: Data notification icons will display in the Injection column if Compass for iCE detects a potential analysis issue or data was manually modified by the user. For more information see “Data Notifications and Warnings” on page 260. User Guide for Maurice, Maurice C. and Maurice S.
Page 256: Graph Pane: Electropherogram Data
Results shown will either be for one injection or multiple injections, samples or standards depending on the view options you’re using. Check out “Viewing Run Data” on page 251 for more info. User Guide for Maurice, Maurice C. and Maurice S.
Page 257 100 (shown for named peak sample data only). User Guide for Maurice, Maurice C. and Maurice S.
Page 258 - Left half peak width at half maximum peak height of Peak B in seconds. • - Migration time of Peak A in seconds in Peaks Table. • - Migration time of Peak B in seconds in Peaks Table. User Guide for Maurice, Maurice C. and Maurice S.
Page 259: Injections Pane: User-Specified Peak Names
The Injections pane shows tabulated results for sample proteins Compass for iCE labels automatically using user-defined peak name settings. Each row in the table shows the individual results for the named peaks detected in each injection. User Guide for Maurice, Maurice C. and Maurice S.
Page 260 100. NOTE: The sum of the named peak percentages can be less than 100% if some peaks aren’t named. User Guide for Maurice, Maurice C. and Maurice S.
Page 261: Viewing Run Data
Samples. • Data in this view is for sample proteins only. • The graph displays electropherograms with a y-axis of Absorbance units (mAU) and an x-axis of RMT (relative migration time). User Guide for Maurice, Maurice C. and Maurice S.
Page 262 Data in this view is for analyzing standards only. This is the Internal Standard you add to your sam- ples during prep. • The graph displays electropherograms with a y-axis of Absorbance units (mAU) and an x-axis of time in seconds. User Guide for Maurice, Maurice C. and Maurice S.
Page 263 The Internal Standard is identified in the Peaks pane with an and as IS in the Injections pane. For information on checking and identifying the Internal Standard peak, see “Checking Your Data” on page 177. User Guide for Maurice, Maurice C. and Maurice S.
Page 264: Selecting And Displaying Injection Data
You can view data from one, multiple, or all injections at once. • To look at data for one injection - Click an injection row in the Experiment pane. Data for just that injection displays in the graph and tables. User Guide for Maurice, Maurice C. and Maurice S.
Page 265 To look at data for specific injections - Hold the Ctrl key and select just the injection rows you want to view in the Experiment pane. Data for only the injections selected display in the graph and tables. User Guide for Maurice, Maurice C. and Maurice S.
Page 266 Shift key and select the last. This selects all rows between the two injections. Data for only the injections selected display in the graph and tables. User Guide for Maurice, Maurice C. and Maurice S.
Page 267: Switching Between Single And Multiple Views Of
Selected. Data for the injection row(s) selected in the Experiment pane: • Displays with electropherograms either overlaid or stacked in the Graph pane depending on the option you’ve got chosen. User Guide for Maurice, Maurice C. and Maurice S.
Page 268 Data for the injection row(s) selected in the Experiment pane: • Displays with the electropherograms of the selected injections highlighted in the Graph pane. • Shows the results for the selected injections highlighted in the Peaks and Injections panes. User Guide for Maurice, Maurice C. and Maurice S.
Page 269: Hiding Injection Data
To hide injections - Select the injection rows you want to hide in the Experiment pane, then right click one and select Hide. Data for the injections will be hidden in all data views and results tables. User Guide for Maurice, Maurice C. and Maurice S.
Page 270: Data Notifications And Warnings
“Step 1: Check Your Internal Standard” on page 177. Roll your mouse over the icon to display warning details. User Guide for Maurice, Maurice C. and Maurice S.
Page 271: Checking Your Results
Please see the step by step procedure in “Checking Your Data” on page 177 to do this. If you see a data warning in the Experiment pane, these steps will also help you identify and correct any issues. User Guide for Maurice, Maurice C. and Maurice S.
Page 272: Group Statistics
2. Go back to the Analysis screen. Click View in the main menu and select Grouping. NOTE: To turn Grouping off, select View in the main menu and deselect Grouping. User Guide for Maurice, Maurice C. and Maurice S.
Page 273: Viewing Sample Injection Groups
%CV and SEM (standard error mea- surement). The number in parenthesis after the sample name is the number of injections in the group. User Guide for Maurice, Maurice C. and Maurice S.
Page 274 Collapse All (-) in the upper right corner of the pane. The Method Groups pane pivots the Peak Groups pane results to show statistics for named protein peaks in individual columns. User Guide for Maurice, Maurice C. and Maurice S.
Page 275: Hiding Or Removing Injections In Group
Hidden injections are not included in injection groups. But, hiding injections gives you an easy way to reject individual injections from the statistical analysis. See “Hiding Injection Data” on page 259 for details on how to do this. User Guide for Maurice, Maurice C. and Maurice S.
Page 276: Copying Results Tables And Graphs
Copy, or right click in the Graph pane and select Copy. 3. Select an image option (EMF, PNG or PDF) in the pop-up window, then click Save. User Guide for Maurice, Maurice C. and Maurice S.
Page 277: Exporting Run Files
NOTE: To exclude export of standards data or export results table data in .csv format, see “Setting Data Export Options” on page 435. Exporting Raw Sample Electropherogram Data To export raw sample plot and background data: 1. Click File in the main menu and click Export Spectra. User Guide for Maurice, Maurice C. and Maurice S.
Page 278: Changing Sample Protein Identification
• To remove a peak from the data - Right click the peak in the electropherogram or Peaks table and select Remove peak. The software will no longer identify it as a sample peak in the elec- tropherogram, and the peak data will be removed in the results tables. User Guide for Maurice, Maurice C. and Maurice S.
Page 279 A check mark will appear next to the injection in the Experiment pane to indicate a manual cor- rection was made. User Guide for Maurice, Maurice C. and Maurice S.
Page 280: Hiding Sample Data
3. Click on the row in the experiment pane that contains the injection you want to correct, then click the Graph tab. 4. Right click the peak in the electropherogram or Peaks table and select Hide. Compass for iCE will hide the peak data in the results tables. User Guide for Maurice, Maurice C. and Maurice S.
Page 281: Changing Peak Names For Sample Data
View bar. 2. Click Single View in the View bar. 3. Click on the row in the experiment pane that has the sample you want to correct, then click the Graph pane. User Guide for Maurice, Maurice C. and Maurice S.
Page 282: Changing The Electropherogram View
Options in the Graph pane let you zoom and rescale electropherograms, overlay or stack plots and change the peak and plot info displayed. The Graph pane toolbar has these options: Auto Scale Graph Options Stack the Plots Overlay the Plots User Guide for Maurice, Maurice C. and Maurice S.
Page 283: Autoscaling The Electropherogram
Customizing the Data Display You can customize electropherogram peak labels, plot labels and display options. To do this, just select the Graph Options button. User Guide for Maurice, Maurice C. and Maurice S.
Page 284 Matching Peak Names - Checking this box will draw vertical lines through each named peak. Using this option with Stack the Plots or Overlay the Plots features is helpful for visually comparing your named peaks across multiple injections. User Guide for Maurice, Maurice C. and Maurice S.
Page 285 • Peak Values - Checking this box will display the molecular weight labels on all peaks in the electro- pherogram. If a MW Marker wasn’t run, RMT values are displayed. User Guide for Maurice, Maurice C. and Maurice S.
Page 286 Fitted peaks - Checking this box displays how the peaks were fit by the software. For CE-SDS runs, the software uses Dropped Lines by default. NOTE: This option is only available for sample data. User Guide for Maurice, Maurice C. and Maurice S.
Page 287 Baseline Fit - Checking this box displays the calculated baseline for the peaks. Baseline points will also display for regions of the electropherogram considered to be at baseline. NOTE: This option is only available for sample data. User Guide for Maurice, Maurice C. and Maurice S.
Page 288 Method - Checking this box displays the method used for the injection. • Injection - Checking this box displays the injection number. For example, I4 for injection 4 in the run. Here’s an example of an electropherogram with all plot labels selected: User Guide for Maurice, Maurice C. and Maurice S.
Page 289: Stacking Multiple Electropherograms
View. 2. Select multiple injection rows in the Experiment pane. 3. Click the Stack the Plots button. The individual electropherograms for each injection you selected will stack in the Graph pane. User Guide for Maurice, Maurice C. and Maurice S.
Page 290: Overlaying Multiple Electropherograms
Graph pane. You can also customize the colors used for the overlay plot display. To do that go to “Setting up Automatic Injection Reports” on page 436. User Guide for Maurice, Maurice C. and Maurice S.
Page 291: Zooming
To zoom in on a specific area of the electropherogram, hold the mouse button down and draw a box around the area with your mouse: To return to default scaling, right click in the electropherogram and click Zoom Out. User Guide for Maurice, Maurice C. and Maurice S.
Page 292: Selecting Data Viewing Options
Sample Raw - Clicking this option displays the basic detector values used to calculate peak absor- bance. To view this you’ll need to select Auto Scale in the Graph pane tool bar. User Guide for Maurice, Maurice C. and Maurice S.
Page 293 Sample Background - Clicking this option displays the basic detector values used to calculate base- line absorbance. To view this you’ll need to select Auto Scale in the Graph pane tool bar. User Guide for Maurice, Maurice C. and Maurice S.
Page 294 (zeroed). This is the default view. • Baseline Fit - Clicking this option displays the calculated baseline for the raw sample data. In this next example, both Baseline Fit and Sample are selected. User Guide for Maurice, Maurice C. and Maurice S.
Page 295 Baseline Points - Clicking this option displays regions of the electropherogram considered to be at baseline. In this example, both Baseline Points and Sample are selected. NOTE: This option is selected automatically when Baseline Fit is selected in graph options. User Guide for Maurice, Maurice C. and Maurice S.
Page 296: Adding And Removing Baseline Points
In this example, both Fit Baseline Corrected and Sample are selected, the fit plot is on the bottom. Adding and Removing Baseline Points Points in the baseline can be added or removed as needed. User Guide for Maurice, Maurice C. and Maurice S.
Page 297: Selecting The Graph X-Axis Range
Clear All. Selecting the Graph X-axis Range The RMT (relative migration time) range used for the x-axis can be changed. Just select View in the main menu and click View Region. User Guide for Maurice, Maurice C. and Maurice S.
Page 298 • Custom lets you manually enter the lower and upper range settings to display in the electrophero- gram. In this example the lower and upper range settings are 0.9 and 2.0. User Guide for Maurice, Maurice C. and Maurice S.
Page 299: Closing Run Files
To close one run file - In the Experiment pane, click on one of the sample rows in the file. Then click File from the main menu and click Close. • To close all open run files - Select File from the main menu and click Close All. User Guide for Maurice, Maurice C. and Maurice S.
Page 300: Analysis Settings Overview
Analysis. If more than one run file is open, select the run file you want to view settings for from the list: This opens the Analysis window: User Guide for Maurice, Maurice C. and Maurice S.
Page 301 • Click to save changes to the run file and exit. • Click Cancel to exit without saving changes. User Guide for Maurice, Maurice C. and Maurice S.
Page 302: Advanced Analysis Settings
Allowable Drift - The distance the Internal Standard is expected to move compared to the entered number of seconds on the Markers page. This setting helps with recognition of the Internal Standard. User Guide for Maurice, Maurice C. and Maurice S.
Page 303: Advanced Analysis Settings Groups
> Analysis, and select Advanced in the left sidebar. 2. Click under the analysis settings box. A new group will be created: 3. Click on the new group and enter a new name. User Guide for Maurice, Maurice C. and Maurice S.
Page 304: Changing The Default Analysis Group
> Analysis, and select Advanced in the left sidebar. 2. Click the arrow in the drop down list next to Apply Default, then click a new default group from the list. User Guide for Maurice, Maurice C. and Maurice S.
Page 305: Modifying An Analysis Group
Deleting an Analysis Group 1. Select Edit > Analysis, and select Advanced in the left sidebar. 2. Click on the group in the analysis settings box you want to delete and click Remove. User Guide for Maurice, Maurice C. and Maurice S.
Page 306: Applying Analysis Groups To Specific Run Data
A default override data set will be created from sample information found in the run file. 4. Click the cell in the Apply To column, then click the down arrow. User Guide for Maurice, Maurice C. and Maurice S.
Page 307 6. If you need to change the analysis group used for a data set, click the cell in the Settings column and click the down arrow. Select a group from the drop down list. User Guide for Maurice, Maurice C. and Maurice S.
Page 308: Markers Analysis Settings
NOTE: Settings can be changed in the batch default analysis before you start the run, or in run files once they’re completed. If you make analysis settings changes to an executing run, they won’t be saved to the final run file. User Guide for Maurice, Maurice C. and Maurice S.
Page 309: Markers Settings
CE-SDS MW Markers in, or opt to not use one. NOTE: When the markers injection is set to no markers, the molecular weight for sample proteins in the run isn’t displayed. User Guide for Maurice, Maurice C. and Maurice S.
Page 310: Standards Analysis Settings Groups
Analysis settings are run-file specific. But, settings can be imported or exported for use with other run files. For more information see “Importing and Exporting Analysis Settings” on page 333. Standards groups are displayed in the analysis settings box: User Guide for Maurice, Maurice C. and Maurice S.
Page 311: Creating A New Standards Group
4. Change the Internal Standard time as needed. 5. Click the arrow in the drop down list next to Markers Injection, then click an injection number or no markers from the list. User Guide for Maurice, Maurice C. and Maurice S.
Page 312 Click in the first cell in the MW column in the table and enter the molecular weight (in kDa) for the marker. b. Click in the first cell in the RMT column and enter a value for the marker. User Guide for Maurice, Maurice C. and Maurice S.
Page 313 7. To use the new group as the default settings for the run, click the arrow in the drop down list next to Apply Default, then click the new group in the list. The settings in the new group will then be applied to the run data. 8. Click to save changes. User Guide for Maurice, Maurice C. and Maurice S.
Page 314: Changing The Default Standards Group
Deleting a Standards Group 1. Select Edit > Analysis, and click Markers in the left sidebar. 2. Click on the group in the analysis settings box you want to delete and click Remove. User Guide for Maurice, Maurice C. and Maurice S.
Page 315: Applying Standards Groups To Specific Run
A default override data set will be created from sample information found in the run file. 4. Click the cell in the Apply To column, then click the down arrow. User Guide for Maurice, Maurice C. and Maurice S.
Page 316 6. If you need to change the analysis group used for a data set, click the cell in the Settings column and click the down arrow. Select a group from the drop down list. User Guide for Maurice, Maurice C. and Maurice S.
Page 317: Peak Fit Analysis Settings
Peak Fit Analysis Settings This page lets you view and change peak fit settings for sample data. Select Edit in the main menu and click Analysis, then click Peak Fit in the left sidebar: User Guide for Maurice, Maurice C. and Maurice S.
Page 318: Range Settings
View - Sets the default range to either Full or Analysis for the electropherogram x-axis range in the View Region window (select View in the main menu and click View Region). User Guide for Maurice, Maurice C. and Maurice S.
Page 319: Baseline Settings
It draws two vertical lines from the left and right bounds of the peak down to the x-axis and then measures the total area bounded by the signal curve, the x-axis (y=0 line), and the two vertical lines. User Guide for Maurice, Maurice C. and Maurice S.
Page 320: Peak Fit Analysis Settings Groups
Peak fit settings are saved as a group, and you can create multiple settings groups. Specific group settings can then be applied to methods, injections, sample names or other attributes in the run data. User Guide for Maurice, Maurice C. and Maurice S.
Page 321: Creating A New Peak Fit Group
> Analysis, and select Peak Fit in the left sidebar. 2. Click under the analysis settings box. A new group will be created: 3. Click on the new group and enter a new name. User Guide for Maurice, Maurice C. and Maurice S.
Page 322 5. To use the new group as the default peak fit settings for the run file data, click the arrow in the drop down list next to Apply Default, then click the new group from the list. Peak fit settings in the new group will then be applied to the run data. User Guide for Maurice, Maurice C. and Maurice S.
Page 323: Changing The Default Peak Fit Group
2. Click on the group in the analysis settings box you want to modify. 3. Change the settings in the range, baseline or peak find boxes as needed. User Guide for Maurice, Maurice C. and Maurice S.
Page 324: Deleting A Peak Fit Group
1. Select Edit > Analysis, and select Peak Fit in the left sidebar. 2. Click on the group in the analysis settings box you want to apply to specific run data. User Guide for Maurice, Maurice C. and Maurice S.
Page 325 Sample names - All sample names in the run file display in the list, otherwise the default name of Sample shows. Selecting a sample name applies the group settings to all injections that used that sample name. User Guide for Maurice, Maurice C. and Maurice S.
Page 326 7. Repeat the previous steps to apply other groups to specific run data. 8. To remove a data set, click on its cell in the Apply To column, then click Remove. 9. Click to save changes. User Guide for Maurice, Maurice C. and Maurice S.
Page 327: Manual Peak Integration
4. Select an injection in the Experiment pane. 5. Hover the mouse over the peak you want to adjust. Two magenta bars will display that indicate the start and end points of its integration. User Guide for Maurice, Maurice C. and Maurice S.
Page 328 If the cursor changes to this is the peak end for the peak on the left. • If the cursor changes to this is a joint boundary for the peaks on the left and right. User Guide for Maurice, Maurice C. and Maurice S.
Page 329 You can also separate areas between peaks. Whenever you have a cursor between two peaks that aren’t baseline resolved, move the mouse slightly to the right or left until you get the cursor. User Guide for Maurice, Maurice C. and Maurice S.
Page 330 7. To remove the manual integration settings, right click on the graph or on the injection in the Experi- ment pane and select Clear. User Guide for Maurice, Maurice C. and Maurice S.
Page 331: Peak Names Settings
Peak name settings are saved as a group, and you can create multiple settings groups. Specific group set- tings can be applied to methods, injections, sample names or other attributes in the run data. User Guide for Maurice, Maurice C. and Maurice S.
Page 332: Creating A Peak Names Group
Creating a Peak Names Group 1. Select Edit > Analysis, and select Peak Names in the left sidebar. 2. Click under the analysis settings box. 3. Enter a new name for the group. User Guide for Maurice, Maurice C. and Maurice S.
Page 333 5. Click in the first cell in the column and enter the relative migration time for the sample protein. 6. Click in the first cell in the Color column, then click the button. User Guide for Maurice, Maurice C. and Maurice S.
Page 334 1.8 and 2.2 will be identified with this peak name and color. 10. To add another sample protein, click under the peak table. Repeat the previous steps for other sam- ple proteins. In this example, three proteins were entered: User Guide for Maurice, Maurice C. and Maurice S.
Page 335: Modifying A Peak Names Group
> Analysis, then click Peak Names in the left sidebar. 2. Click on the group in the analysis settings box you want to delete and click Remove. 3. Click to save changes. User Guide for Maurice, Maurice C. and Maurice S.
Page 336: Applying Peak Names Groups To Run Data
4. Click the cell in the Apply To column, then click the down arrow. 5. Select an option from the drop down list. This applies the peak names group selected to specific run data as follows: User Guide for Maurice, Maurice C. and Maurice S.
Page 337 Remove. 9. Click to save changes. Named peaks will be identified with a peak name label in the electrophero- gram and color-coded in the Peaks and Injections panes: User Guide for Maurice, Maurice C. and Maurice S.
Page 338: Injection Reports
File > Open Run and select a run file. 2. If you want reports for all injections, skip to the next step. If you only want reports for certain injections, in the Experiment pane: User Guide for Maurice, Maurice C. and Maurice S.
Page 339 The report name defaults to the run file name. If you want to change it, type in the Report Name box to make updates. f. Click OK. User Guide for Maurice, Maurice C. and Maurice S.
Page 340: Example Analysis And Injection Report
Started run: 3170522428_Retest_2017-06-07_17-29-18_KF1077_FreshQC_Maurice CE-SDS 3170522428 Batch: KF1077_FreshQC_Maurice CE-SDS.batch Created By: Jacquelyn Wed 12:55 PM Jun 14, 2017 CDT Software Version: 2.0.8, Build ID: 0606 C:\Users\Jacquelyn\Documents\Compass for iCE\Maurice CE-SDS.mbz Computer: DESKTOP-C7FPQGB Page 1 of 1 User Guide for Maurice, Maurice C. and Maurice S.
Page 341 1139.6 61.1 16.5 745.3 1.88 Created By: Jacquelyn Wed 12:55 PM Jun 14, 2017 CDT Software Version: 2.0.8, Build ID: 0606 C:\Users\Jacquelyn\Documents\Compass for iCE\Maurice CE-SDS.mbz Computer: DESKTOP-C7FPQGB Page 1 of 2 User Guide for Maurice, Maurice C. and Maurice S.
Page 342 Injections Remaining 196 (96 guaranteed) Created By: Jacquelyn Wed 12:55 PM Jun 14, 2017 CDT Software Version: 2.0.8, Build ID: 0606 C:\Users\Jacquelyn\Documents\Compass for iCE\Maurice CE-SDS.mbz Computer: DESKTOP-C7FPQGB Page 2 of 2 User Guide for Maurice, Maurice C. and Maurice S.
Page 343: Importing And Exporting Analysis Settings
1. Open the run file or batch you want to export analysis settings from. 2. Select Edit in the main menu and click Default Analysis (Batch screen) or Analysis (Analysis screen). 3. Click Export on any page. The following window displays: User Guide for Maurice, Maurice C. and Maurice S.
Page 344 CE-SDS Data Analysis 4. The default directory is Compass for iCE/Runs. Change the directory if needed. 5. Enter a file name and click Save. The settings will be saved as a *.settings file. User Guide for Maurice, Maurice C. and Maurice S.
Page 345: Analysis
Analysis Settings Overview • Advanced Analysis Settings • Detection Settings • Peak Fit Analysis Settings • Manual Peak Integration • Peak Names Settings • pI Markers Analysis Settings • Injection Reports User Guide for Maurice, Maurice C. and Maurice S.
Page 346 336 Chapter 12: cIEF Data Analysis • Importing and Exporting Analysis Settings User Guide for Maurice, Maurice C. and Maurice S.
Page 347: Analysis Screen Overview
Peaks - Shows the tabulated results for sample proteins and pI markers. • Injections - Displays a list of the sample proteins Compass for iCE names automatically using the user-defined peak name analysis parameters. User Guide for Maurice, Maurice C. and Maurice S.
Page 348: Software Menus Active In The Analysis Screen
These main menu items are active in the Analysis Screen: • File • Edit • View • Instrument (when Compass for iCE is connected to Maurice, Maurice C. or Maurice S.) • Window • Help File Menu These File menu options are active: •...
Page 349 Grouping - Displays data for injection groups. • View Region - Lets you change the x-axis range of the data displayed. • Show Hidden- Shows injections that are hidden from the data view. User Guide for Maurice, Maurice C. and Maurice S.
Page 350: Opening Run Files
Open Run. 2. A list of the last 10 runs opened will display. Select one of these runs or click Browse to open the Runs folder and select a different file. User Guide for Maurice, Maurice C. and Maurice S.
Page 351 When a run is added, its data appends to the open run file and displays as a second set of injections in all screen panes. The second run file name also appears in the title bar: 5. Repeat the last two steps to add additional runs. User Guide for Maurice, Maurice C. and Maurice S.
Page 352: How Run Data Is Displayed
For more information see “Data Notifications and Warnings” on page 355. Graph Pane: Electropherogram Data The Graph pane displays the electropherogram(s) for sample proteins or pI markers depending on the view options you’ve selected. User Guide for Maurice, Maurice C. and Maurice S.
Page 353: Peaks Pane: Calculated Results
M. • To view all rows - Use the scroll bar or click Maximize in the upper right corner. User Guide for Maurice, Maurice C. and Maurice S.
Page 354 Resolution - Displays resolution of the peak compared to neighboring peaks. Two peaks that are baseline resolved will have a resolution value of 1.5. Smaller values means the peaks are not com- pletely resolved, larger values mean the peaks are fully resolved. User Guide for Maurice, Maurice C. and Maurice S.
Page 355: Injections Pane: User-Specified Peak Names
This value results from dividing the individual peak area by the sum of all named peak areas for the injection and multiplying by 100. User Guide for Maurice, Maurice C. and Maurice S.
Page 356: Viewing Run Data
To view sample data - Click Samples in the View bar or select View in the main menu and click Samples. • Data in this view is for sample proteins only. User Guide for Maurice, Maurice C. and Maurice S.
Page 357 Go to “Detection Settings” on page 395 for more info on how to change the detection method to view either absorbance or native fluorescence data. User Guide for Maurice, Maurice C. and Maurice S.
Page 358 Peaks pane with an and as Mkr in the Injections pane. For information on checking and identifying the pI marker peaks, see “Checking Your Data” on page 138. User Guide for Maurice, Maurice C. and Maurice S.
Page 359: Selecting And Displaying Injection Data
You can view data from one, multiple, or all injections at once. • To look at data for one injection - Click an injection row in the Experiment pane. Data for just that injection displays in the graph and tables. User Guide for Maurice, Maurice C. and Maurice S.
Page 360 To look at data for specific injections - Hold the Ctrl key and select just the injection rows you want to view in the Experiment pane. Data for only the injections selected display in the graph and tables. User Guide for Maurice, Maurice C. and Maurice S.
Page 361 Shift key and select the last. This selects all rows between the two injections. Data for only the injections selected display in the graph and tables. User Guide for Maurice, Maurice C. and Maurice S.
Page 362: Switching Between Single And Multiple Views Of
Single View. Data for the injection row(s) selected in the Experiment pane: • Displays with electropherograms either overlaid or stacked in the Graph pane depending on the option you’ve got chosen. User Guide for Maurice, Maurice C. and Maurice S.
Page 363 Data for the injection row(s) selected in the Experiment pane: • Displays with the electropherograms of the selected injections highlighted in the Graph pane. • Shows the results for the selected injections highlighted in the Peaks and Injections panes. User Guide for Maurice, Maurice C. and Maurice S.
Page 364: Hiding Injection Data
To hide injections - Select the injection rows you want to hide in the Experiment pane, then right click one and select Hide. Data for the injections will be hidden in all data views and results tables. User Guide for Maurice, Maurice C. and Maurice S.
Page 365: Data Notifications And Warnings
You can fix this by manually identifying the pI marker using the steps in “Step 2: Check Your pI Markers” on page 138. Roll your mouse over the icon to display warning details. User Guide for Maurice, Maurice C. and Maurice S.
Page 366: Checking Your Results
You can use the Grouping view to have Compass for iCE do a statistical analysis of named proteins in your injections (see “Manual Peak Integration” on page 406 for more info on setting named peaks up). Statistics for each protein are also plotted for easy comparison. User Guide for Maurice, Maurice C. and Maurice S.
Page 367: Using Groups
Compass for iCE automatically groups all injections using the same sample name together in the Injection Groups pane. • To expand a group - Click the arrow next to a group to see the individual injections in the group and reported data for each User Guide for Maurice, Maurice C. and Maurice S.
Page 368: Viewing Statistics
100 (shown for named peak sample data only). • To expand a group - Click the arrow next to a group to see the individual injections in the group and reported data for each User Guide for Maurice, Maurice C. and Maurice S.
Page 369 The mean values for named peaks using the same method in each injection group are plotted in bar graphs with error bars showing the standard deviation in the Group Plots pane. You’ll also get plots that compare samples using the same method in the run. User Guide for Maurice, Maurice C. and Maurice S.
Page 370: Hiding Or Removing Injections In Group
Hidden injections are not included in injection groups. But, hiding injections gives you an easy way to reject individual injections from the statistical analysis. See “Hiding Injection Data” on page 354 for details on how to do this. User Guide for Maurice, Maurice C. and Maurice S.
Page 371: Copying Results Tables And Graphs
Copy, or right click in the Graph pane and select Copy. 3. Select an image option (EMF, PNG or PDF) in the pop-up window, then click Save. User Guide for Maurice, Maurice C. and Maurice S.
Page 372: Exporting Run Files
Data Export Options” on page 435. Exporting Raw Sample Electropherogram Data To export raw sample plot and background data: 1. Click File in the main menu and click Export Spectra. User Guide for Maurice, Maurice C. and Maurice S.
Page 373: Changing Sample Protein Identification
• To remove a peak from the data - Right click the peak in the electropherogram or Peaks table and select Remove peak. The software will no longer identify it as a sample peak in the elec- tropherogram, and the peak data will be removed in the results tables. User Guide for Maurice, Maurice C. and Maurice S.
Page 374 NOTE: To remove sample peak assignments that were made manually and go back to the original peak data, right-click the peak in the electropherogram and select Clear for the current injection or Clear All for all injections in the batch. User Guide for Maurice, Maurice C. and Maurice S.
Page 375: Hiding Sample Data
3. Click on the row in the experiment pane that contains the injection you want to correct, then click the Graph tab. 4. Right click the peak in the electropherogram or Peaks table and select Hide. Compass for iCE will hide the peak data in the results tables. User Guide for Maurice, Maurice C. and Maurice S.
Page 376: Changing Peak Names For Sample Data
View bar. 2. Click Single View in the View bar. 3. Click on the row in the experiment pane that has the sample you want to correct, then click the Graph pane. User Guide for Maurice, Maurice C. and Maurice S.
Page 377: Changing The Electropherogram View
Options in the Graph pane let you zoom and rescale electropherograms, overlay or stack plots and change the peak and plot info displayed. The Graph pane toolbar has these options: Auto Scale Graph Options Stack the Plots Overlay the Plots User Guide for Maurice, Maurice C. and Maurice S.
Page 378: Autoscaling The Electropherogram
Customizing the Data Display You can customize electropherogram peak labels, plot labels and display options. To do this, just select the Graph Options button. User Guide for Maurice, Maurice C. and Maurice S.
Page 379 Matching Peak Names - Checking this box will draw vertical lines through each named peak. Using this option with Stack the Plots or Overlay the Plots features is helpful for visually comparing your named peaks across multiple injections. User Guide for Maurice, Maurice C. and Maurice S.
Page 380 NOTE: If more than one peak label option is selected, peak name labels will always be used for named peaks. • Peak Values - Checking this box will display the pI values on all peaks in the electropherogram. User Guide for Maurice, Maurice C. and Maurice S.
Page 381 Fitted peaks - Checking this box displays how the peaks were fit by the software. For cIEF runs, the software uses Gaussian Fit by default. NOTE: This option is only available for sample data. User Guide for Maurice, Maurice C. and Maurice S.
Page 382 Baseline Fit - Checking this box displays the calculated baseline for the peaks. Baseline points will also display for regions of the electropherogram considered to be at baseline. NOTE: This option is only available for sample data. User Guide for Maurice, Maurice C. and Maurice S.
Page 383 Exposure - Checking this box display the exposure time(s) used for the data. • Injection - Checking this box displays the injection number. For example, I4 for injection 4 in the run. Here’s an example of an electropherogram with all plot labels selected: User Guide for Maurice, Maurice C. and Maurice S.
Page 384: Stacking Multiple Electropherograms
View. 2. Select multiple injection rows in the Experiment pane. 3. Click the Stack the Plots button. The individual electropherograms for each injection you selected will stack in the Graph pane. User Guide for Maurice, Maurice C. and Maurice S.
Page 385: Overlaying Multiple Electropherograms
2. Select an injection row in the Experiment pane. 3. Click Graph Options and select either Overlay Abs Overlay FL. The option available depends on what detection mode is currently displaying. User Guide for Maurice, Maurice C. and Maurice S.
Page 386 4. The electropherograms for each detection mode will overlay in the Graph pane, and the other detection mode y axis will display on the right. NOTE: It’ s helpful to turn on Auto Scale when you’re comparing the traces. User Guide for Maurice, Maurice C. and Maurice S.
Page 387: Zooming
To zoom in on a specific area of the electropherogram, hold the mouse button down and draw a box around the area with your mouse: To return to default scaling, right click in the electropherogram and click Zoom Out. User Guide for Maurice, Maurice C. and Maurice S.
Page 388: Selecting Data Viewing Options
NOTE: Unless noted otherwise, graph view menu options are available for sample data only. • Sample Raw - Clicking this option displays the basic detector values used to calculate peak absor- bance or peak fluorescence depending on which mode is being viewed. User Guide for Maurice, Maurice C. and Maurice S.
Page 389 Changing the Electropherogram View page 379 • Sample Background - Clicking this option displays the basic detector values used to calculate base- line absorbance or peak fluorescence depending on which mode is being viewed. User Guide for Maurice, Maurice C. and Maurice S.
Page 390 (zeroed). This is the default view. In this next example, both Sample and Sample Baseline Corrected are selected. • Baseline Fit - Clicking this option displays the calculated baseline for the raw sample data. In this next example, both Baseline Fit and Sample are selected. User Guide for Maurice, Maurice C. and Maurice S.
Page 391 Baseline Points - Clicking this option displays regions of the electropherogram considered to be at baseline. In this example, both Baseline Points and Sample are selected. NOTE: This option is selected automatically when Baseline Fit is selected in graph options. User Guide for Maurice, Maurice C. and Maurice S.
Page 392: Adding And Removing Baseline Points
In this example, both Fit Baseline Corrected and Sample Raw are selected, the fit plot is on the bottom. Adding and Removing Baseline Points Points in the baseline can be added or removed as needed. User Guide for Maurice, Maurice C. and Maurice S.
Page 393: Selecting The Graph X-Axis Range
Clear All. Selecting the Graph X-axis Range The pI range used for the x-axis can be changed. Just select View in the main menu and click View Region. User Guide for Maurice, Maurice C. and Maurice S.
Page 394 • Custom lets you manually enter the lower and upper range settings to display in the electrophero- gram. In this example the lower and upper range settings are 5.0 and 9.0. User Guide for Maurice, Maurice C. and Maurice S.
Page 395: Closing Run Files
To close one run file - In the Experiment pane, click on one of the sample rows in the file. Then click File from the main menu and click Close. • To close all open run files - Select File from the main menu and click Close All. User Guide for Maurice, Maurice C. and Maurice S.
Page 396: Analysis Settings Overview
Analysis. If more than one run file is open, select the run file you want to view settings for from the list: This opens the Analysis window: User Guide for Maurice, Maurice C. and Maurice S.
Page 397 • Click to save changes to the run file and exit. • Click Cancel to exit without saving changes. User Guide for Maurice, Maurice C. and Maurice S.
Page 398: Advanced Analysis Settings
Allowable Drift - The distance the pI marker(s) are expected to move compared to the position entered on the pI Markers page. This setting helps with recognition of the pI marker. User Guide for Maurice, Maurice C. and Maurice S.
Page 399: Advanced Analysis Settings Groups
> Analysis, and select Advanced in the left sidebar. 2. Click under the analysis settings box. A new group will be created: 3. Click on the new group and enter a new name. User Guide for Maurice, Maurice C. and Maurice S.
Page 400: Changing The Default Analysis Group
> Analysis, and select Advanced in the left sidebar. 2. Click the arrow in the drop down list next to Apply Default, then click a new default group from the list. User Guide for Maurice, Maurice C. and Maurice S.
Page 401: Modifying An Analysis Group
Deleting an Analysis Group 1. Select Edit > Analysis, and select Advanced in the left sidebar. 2. Click on the group in the analysis settings box you want to delete and click Remove. User Guide for Maurice, Maurice C. and Maurice S.
Page 402: Applying Analysis Groups To Specific Run Data
A default override data set will be created from sample information found in the run file. 4. Click the cell in the Apply To column, then click the down arrow. User Guide for Maurice, Maurice C. and Maurice S.
Page 403 6. If you need to change the analysis group used for a data set, click the cell in the Settings column and click the down arrow. Select a group from the drop down list. User Guide for Maurice, Maurice C. and Maurice S.
Page 404 7. Repeat the previous steps to apply other groups to specific run data. 8. To remove a data set, click on its cell in the Apply To column, then click Remove. 9. Click to save changes. User Guide for Maurice, Maurice C. and Maurice S.
Page 405: Detection Settings
You can choose to display either absorbance or fluorescence data for your run in the Analysis screen. 1. Select Edit > Analysis, and select Detection in the left sidebar. 2. Select either the Absorbance or Fluorescence radio button. User Guide for Maurice, Maurice C. and Maurice S.
Page 406: Changing The Detection Exposure
1. Select Edit > Analysis, and select Detection in the left sidebar. 2. Click the arrow in the exposure button you wan to change and select an exposure setting: User Guide for Maurice, Maurice C. and Maurice S.
Page 407: Peak Fit Analysis Settings
NOTE: Settings can be changed in the batch default analysis before you start the run, or in run files once they’re completed. If you make analysis settings changes to an executing run, they won’t be saved to the final run file. User Guide for Maurice, Maurice C. and Maurice S.
Page 408: Range Settings
Width - The approximate peak width (at full width half max) in pixels used to detect peaks. The mini- mum value for this setting is 3.0. Larger widths help eliminate the detection of shoulder and noise peaks. User Guide for Maurice, Maurice C. and Maurice S.
Page 409 It draws two vertical lines from the left and right bounds of the peak down to the x-axis and then measures the total area bounded by the signal curve, the x-axis (y=0 line), and the two vertical lines. User Guide for Maurice, Maurice C. and Maurice S.
Page 410: Peak Fit Analysis Settings Groups
> Analysis, and select Peak Fit in the left sidebar. 2. Click under the analysis settings box. A new group will be created: 3. Click on the new group and enter a new name. User Guide for Maurice, Maurice C. and Maurice S.
Page 411 5. To use the new group as the default peak fit settings for the run file data, click the arrow in the drop down list next to Apply Default, then click the new group from the list. Peak fit settings in the new group will then be applied to the run data. User Guide for Maurice, Maurice C. and Maurice S.
Page 412: Changing The Default Peak Fit Group
2. Click on the group in the analysis settings box you want to modify. 3. Change the settings in the range, baseline or peak find boxes as needed. User Guide for Maurice, Maurice C. and Maurice S.
Page 413: Deleting A Peak Fit Group
1. Select Edit > Analysis, and select Peak Fit in the left sidebar. 2. Click on the group in the analysis settings box you want to apply to specific run data. User Guide for Maurice, Maurice C. and Maurice S.
Page 414 Sample names - All sample names in the run file display in the list, otherwise the default name of Sample shows. Selecting a sample name applies the group settings to all injections that used that sample name. User Guide for Maurice, Maurice C. and Maurice S.
Page 415 7. Repeat the previous steps to apply other groups to specific run data. 8. To remove a data set, click on its cell in the Apply To column, then click Remove. 9. Click to save changes. User Guide for Maurice, Maurice C. and Maurice S.
Page 416: Manual Peak Integration
4. Select an injection in the Experiment pane. 5. Hover the mouse over the peak you want to adjust. Two magenta bars will display that indicate the start and end points of its integration. User Guide for Maurice, Maurice C. and Maurice S.
Page 417 If the cursor changes to this is the peak end for the peak on the left. • If the cursor changes to this is a joint boundary for the peaks on the left and right. User Guide for Maurice, Maurice C. and Maurice S.
Page 418 In the example below, we moved the start and end points of the peak to include more area under the peak: You can also combine peaks by dragging the magenta lines left or right: User Guide for Maurice, Maurice C. and Maurice S.
Page 419 7. To remove the manual integration settings, right click on the graph or on the injection in the Experi- ment pane and select Clear. User Guide for Maurice, Maurice C. and Maurice S.
Page 420 410 Chapter 12: cIEF Data Analysis User Guide for Maurice, Maurice C. and Maurice S.
Page 421: Peak Names Settings
Peak name settings are saved as a group, and you can create multiple settings groups. Specific group set- tings can be applied to methods, injections, sample names or other attributes in the run data. User Guide for Maurice, Maurice C. and Maurice S.
Page 422: Creating A Peak Names Group
Creating a Peak Names Group 1. Select Edit > Analysis, and select Peak Names in the left sidebar. 2. Click under the analysis settings box. 3. Enter a new name for the group. User Guide for Maurice, Maurice C. and Maurice S.
Page 423 5. Click in the first cell in the column and enter the expected pI for the sample protein. 6. Click in the first cell in the Color column, then click the button. User Guide for Maurice, Maurice C. and Maurice S.
Page 424 2.2 will be identified with this peak name and color. 10. To add another sample protein, click under the peak table. Repeat the previous steps for other sam- ple proteins. In this example, eight proteins were entered: User Guide for Maurice, Maurice C. and Maurice S.
Page 425: Modifying A Peak Names Group
Deleting a Peak Names Group 1. Select Edit > Analysis, then click Peak Names in the left sidebar. 2. Click on the group in the analysis settings box you want to delete and click Remove. User Guide for Maurice, Maurice C. and Maurice S.
Page 426: Applying Peak Names Groups To Run Data
You can either modify the default group or click under the box to create a new one. 4. Click the cell in the Apply To column, then click the down arrow. User Guide for Maurice, Maurice C. and Maurice S.
Page 427 6. If you need to change the peak names group used for a data set, click the cell in the Settings column and click the down arrow. Select a group from the drop down list. User Guide for Maurice, Maurice C. and Maurice S.
Page 428 Remove. 9. Click to save changes. Named peaks will be identified with a peak name label in the electrophero- gram and color-coded in the Peaks and Injections panes: User Guide for Maurice, Maurice C. and Maurice S.
Page 429: Pi Markers Analysis Settings
Specific group settings can be applied to methods, injections, sample names or other attributes in the run data. User Guide for Maurice, Maurice C. and Maurice S.
Page 430: Creating A New Markers Group
> Analysis, and select pI Markers in the left sidebar. 2. Click under the analysis settings box. A new group will be created: 3. Click on the new group and enter a new name. User Guide for Maurice, Maurice C. and Maurice S.
Page 431 Click in the first cell in the pI column in the table and enter the pI for the marker. b. Click in the first cell in the Position column and enter a value for the marker. User Guide for Maurice, Maurice C. and Maurice S.
Page 432: Changing The Default Markers Group
> Analysis, and click pI Markers in the left sidebar. 2. Click the arrow in the drop down list next to Apply Default, then select a new default group from the list. User Guide for Maurice, Maurice C. and Maurice S.
Page 433: Modifying A Markers Group
Deleting a Markers Group 1. Select Edit > Analysis, and click pI Markers in the left sidebar. 2. Click on the group in the analysis settings box you want to delete and click Remove. User Guide for Maurice, Maurice C. and Maurice S.
Page 434: Applying Markers Groups To Specific Run Data
A default override data set will be created from sample information found in the run file. 4. Click the cell in the Apply To column, then click the down arrow. User Guide for Maurice, Maurice C. and Maurice S.
Page 435 6. If you need to change the analysis group used for a data set, click the cell in the Settings column and click the down arrow. Select a group from the drop down list. User Guide for Maurice, Maurice C. and Maurice S.
Page 436: Injection Reports
To select specific injections: Hold the Ctrl key and select just the injections you want reports for. 3. Select File from the main menu in either screen and click Injection Report. User Guide for Maurice, Maurice C. and Maurice S.
Page 437 5. The Injection Report PDF(s) are exported to the Runs folder in the Compass for iCE directory. They’ll be in a folder with the report name used in the prior step. When the reports are done, the folder opens for you automatically. User Guide for Maurice, Maurice C. and Maurice S.
Page 438: Example Analysis And Injection Report
Saved as Maurice cIEF from Compass for iCE v2.0.8-0606 Created By: Jacquelyn Wed 12:59 PM Jun 14, 2017 CDT Software Version: 2.0.8, Build ID: 0606 C:\Users\Jacquelyn\Documents\Compass for iCE\Maurice cIEF.mbz Computer: DESKTOP-C7FPQGB Page 1 of 1 User Guide for Maurice, Maurice C. and Maurice S.
Page 439 10.170 173.0 1537 0.0408 580.6 3.16 Created By: Jacquelyn Wed 12:59 PM Jun 14, 2017 CDT Software Version: 2.0.8, Build ID: 0606 C:\Users\Jacquelyn\Documents\Compass for iCE\Maurice cIEF.mbz Computer: DESKTOP-C7FPQGB Page 1 of 2 User Guide for Maurice, Maurice C. and Maurice S.
Page 440 158 (58 guaranteed) Batches Remaining Created By: Jacquelyn Wed 12:59 PM Jun 14, 2017 CDT Software Version: 2.0.8, Build ID: 0606 C:\Users\Jacquelyn\Documents\Compass for iCE\Maurice cIEF.mbz Computer: DESKTOP-C7FPQGB Page 2 of 2 User Guide for Maurice, Maurice C. and Maurice S.
Page 441: Importing And Exporting Analysis Settings
1. Open the run file or batch you want to export analysis settings from. 2. Select Edit in the main menu and click Default Analysis (Batch screen) or Analysis (Analysis screen). 3. Click Export on any page. The following window displays: User Guide for Maurice, Maurice C. and Maurice S.
Page 442 Data Analysis 4. The default directory is Compass for iCE/Runs. Change the directory if needed. 5. Enter a file name and click Save. The settings will be saved as a *.settings file. User Guide for Maurice, Maurice C. and Maurice S.
Page 443: Preferences
Chapter Overview • Customize Your Preferences • Enabling Access Control • Setting Data Export Options • Setting up Automatic Injection Reports • Grouping Options • Setting Up Maurice Systems to Send Tweets User Guide for Maurice, Maurice C. and Maurice S.
Page 444: Chapter 13: Setting Your
Grouping - Groups samples with the same name together across runs, so you can get statistics for the same sample in multiple runs. • Twitter - Lets you configure Compass for iCE to tweet Maurice, Maurice C. and Maurice S. run status. In all preferences windows: User Guide for Maurice, Maurice C. and Maurice S.
Page 445: Enabling Access Control
ANDI CE-SDS Export Type: • Time - Exports the peak detection time • Relative Migration Time (RMT) - Exports the RMT of identified peaks in the CE-SDS run data. • Auto Export Spectra: User Guide for Maurice, Maurice C. and Maurice S.
Page 446: Setting Up Automatic Injection Reports
3. Check any additional reports you’d like to have automatically generated at the end of each run Reports include all injections in the batch, and are saved in \Documents\Compass for iCE\Runs by default. To save reports in another folder, click Browse and select a different location. User Guide for Maurice, Maurice C. and Maurice S.
Page 447: Selecting Custom Plot Colors For Graph Overlay
(this is the default setting). NOTE: If Apply colors to stacked plots isn’t selected, the colors shown are only applied to plots when Overlay the plots is selected in the Graph pane. User Guide for Maurice, Maurice C. and Maurice S.
Page 448 438 Chapter 13: Setting Your Preferences User Guide for Maurice, Maurice C. and Maurice S.
Page 449: Grouping Options
(this is the default setting). NOTE: To activate grouping and get statistics for runs you have open in the Analysis Screen, select View the main menu and click Grouping. User Guide for Maurice, Maurice C. and Maurice S.
Page 450: Setting Up Maurice Systems To Send Tweets
To set your Maurice system up to tweet, the computer you’re using needs to be connected to the internet through a network connection or the local lab computer. We recommend setting up separate Twitter accounts for each system. This lets multiple people in the lab follow run progress. User Guide for Maurice, Maurice C. and Maurice S.
Page 451 Setting Up Maurice Systems to Send Tweets page 441 1. Click Account. A set account window will display in Compass for iCE and a browser window will open: User Guide for Maurice, Maurice C. and Maurice S.
Page 452 3. Enter the PIN number in the Compass for iCE set account window and click OK: 4. The user name will now appear in the Twitter User Name box. Select your Tweet When options and click Apply. User Guide for Maurice, Maurice C. and Maurice S.
Page 453 443 5. To confirm the Twitter account is receiving messages, click Tweet Message. Enter a test message and click OK. 6. If the test Tweet was successful, you’ll get this message: User Guide for Maurice, Maurice C. and Maurice S.
Page 454 444 Chapter 13: Setting Your Preferences 7. Click to save changes and exit. Maurice, Maurice C. and Maurice S. will automatically tweet as the selected options occur: Changing the Twitter Account To change the Twitter account your system uses: 1.
Page 455: Compass Access Control And 21 Cfr Part 11 Compliance
• Enabling Access Control • Logging In to Compass for iCE • Saving Changes • Signing Files • Instrument Command Log • Run File History • Troubleshooting Problems and Suggested Solutions User Guide for Maurice, Maurice C. and Maurice S.
Page 456: Overview
To enable Compass for iCE to use a particular Authorization Server, click Edit, then Preferences Access Control and enter the server IP address using format X.X.X.X. User Guide for Maurice, Maurice C. and Maurice S.
Page 457: Server Administration
After installing the Authorization Server, the administrator user name and password can be changed. NOTE: Sites are responsible for maintaining their own admin and user account credentials. Record these and keep them safe. ProteinSimple can’t provide access once the admin account has been edited. User Guide for Maurice, Maurice C. and Maurice S.
Page 458: Adding Non-Admin Users
Add a user to the server to allow that user to log in to Compass for iCE. To do this: 1. Select Users from the Site Administration home page: 2. From the Users page, select User: User Guide for Maurice, Maurice C. and Maurice S.
Page 459 Authorization Server page 449 3. Fill in the fields to create a new user: After adding a new user more information can be added: User Guide for Maurice, Maurice C. and Maurice S.
Page 460 Compass for iCE to use the new permissions. Users can belong to groups that have multiple permissions such as Operator or Scientist: User Guide for Maurice, Maurice C. and Maurice S.
Page 461 Authorization Server page 451 Use the Groups page to change the permissions in a group or create new groups: To change permissions for a group click Change, then select a group: User Guide for Maurice, Maurice C. and Maurice S.
Page 462: Adding Admin Users
To create a user with administrator permissions: 1. Follow the steps described in "Adding Non-admin Users" on page 448 to create the admin user. 2. Under permissions, select Staff status Superuser status: User Guide for Maurice, Maurice C. and Maurice S.
Page 463: Resetting User Passwords
NOTE: Users are blocked after the number of login failures defined in the Password policy setting. To reset a user password: 1. Select Users from the Site Administration home page, then select the user to change. The following screen displays: User Guide for Maurice, Maurice C. and Maurice S.
Page 464: Audit Trail
3. Enter the new password, then click Change password. Audit Trail Admin users with Staff Status can view, print and download the Audit Trail. Select View Audit Trail from the Site Administration home page to access it. User Guide for Maurice, Maurice C. and Maurice S.
Page 465 Authorization Server page 455 User Guide for Maurice, Maurice C. and Maurice S.
Page 466: Password Policy Settings
LDAP settings allow you to connect the Compass Authorization Server to your own network’s domain con- troller, so users can log on with their existing network password. With LDAP, passwords are not maintained by the Compass Authorization Server, they are administered by the network admin. User Guide for Maurice, Maurice C. and Maurice S.
Page 467: Encryption Details
There is no individual ownership of files, all users who log into Compass for iCE can open any file. User Guide for Maurice, Maurice C. and Maurice S.
Page 468: Enabling Access Control
3. Close Compass for iCE. The next time the software is launched, a user log in will be required. NOTE: Access Control can only be disabled by logging into Compass for iCE and deselecting the Enable box in the Access Control page of Preferences. User Guide for Maurice, Maurice C. and Maurice S.
Page 469: Logging In To Compass For Ice
A successful log in will display the Compass for iCE main window with the user information in the lower sta- tus bar. The full user name is displayed with the unique user ID in parenthesis: User Guide for Maurice, Maurice C. and Maurice S.
Page 470: Locking And Unlocking The Application
Resolving Log In Issues Log in failures may occur when: • The server is temporarily unavailable • Compass for iCE is using the wrong IP address When this happens, the following message displays: User Guide for Maurice, Maurice C. and Maurice S.
Page 471: Saving Changes
File menu, a dialog box will display to allow you to enter a comment before saving the signed file: The comment is added to the signature entry in the file History: User Guide for Maurice, Maurice C. and Maurice S.
Page 472: Signing Files
Approved or Verified. Instrument Command Log The Instrument Command Log can be viewed at any time by selecting the Instrument menu and clicking Properties, and then clicking the Command Log button: User Guide for Maurice, Maurice C. and Maurice S.
Page 473 The Command Log lists all the commands sent to the instrument that were signed by the user who sent the command. If you want to copy the Command Log at any time, right click in the table and select Copy, then paste into another document. User Guide for Maurice, Maurice C. and Maurice S.
Page 474 464 Chapter 14: Compass Access Control and 21 CFR Part 11 Compliance User Guide for Maurice, Maurice C. and Maurice S.
Page 475: Run File History
Ask a Compass for iCE administrator to confirm your user name. If your password is unknown then the administrator can reset your password (see "Resetting User Passwords" on page 453 for more information). User Guide for Maurice, Maurice C. and Maurice S.
Page 476 Access Control is enabled in Preferences. Enable Access Control by clicking Edit, then Preferences Access Control. Select Enable, close Compass for iCE, then re-launch the software. You should now be prompted for a log in. User Guide for Maurice, Maurice C. and Maurice S.
Page 477: Maintenance And Troubleshooting
Chapter Overview • Cartridge Handling and Care • Maintenance • Spare Parts • Software Updates • Instrument Software (Embedded) Updates • Frequently Asked Questions: cIEF Applications • Frequently Asked Questions: CE-SDS Applications User Guide for Maurice, Maurice C. and Maurice S.
Page 478: Cartridge Handling And Care
The cIEF and CE-SDS Cartridges were developed for use with Maurice systems. Each cartridge is individually tested and shipped with a Certificate of Analysis, are also online at http://www.proteinsimple.com/certifi- cates.html. The cartridges are shipped dry and should be stored free of liquid.
Page 479: Cief Cartridge
2. Sweep the air stream across the entire length of the optical window. 3. Flip the cartridge over and repeat the prior steps. User Guide for Maurice, Maurice C. and Maurice S.
Page 480: Ce-Sds Cartridge
If you’ll use the cartridge again within 2 hours of your last run, you can leave the cartridge in Maurice. Otherwise you’ll need to clean and store it. • Always run the cartridge cleanup before storing. See page 175 for the CE-SDS Cartridge cleanup steps. User Guide for Maurice, Maurice C. and Maurice S.
Page 481: Maintenance
Dispose of your samples and reagent vials after each run. Compass for iCE will let you know when a cartridge is at the end of its useful life and should be discarded. Yearly We recommend Maurice has annual preventive maintenance performed by an authorized ProteinSimple engineer. Please contact Technical Support to schedule a visit. Changing the Fuse 1.
Page 482 4. Remove the old fuse. 5. There’s a spare fuse in the small box. Pull the box out, pull the new fuse out and use it to replace defec- tive fuse. User Guide for Maurice, Maurice C. and Maurice S.
Page 483: Spare Parts
7. Plug Maurice’s power cord back in and turn his power on. Spare Parts Maurice, Maurice C. and Maurice S. don’t have any user replaceable parts except for the power fuse. Please contact ProteinSimple Technical Support if they get sick and need repair.
Page 484: Frequently Asked Questions: Cief Applications
Each brand may give slightly different separation resolution due to slight differences in ampholytic composi- tions. Identification of the optimal carrier ampholytes for a given protein sample is best determined experi- mentally. User Guide for Maurice, Maurice C. and Maurice S.
Page 485 IEF, then these additive conditions can often be successfully used for cIEF analysis on Maurice sys- tems. NOTE: All additives may change the pI value of the protein slightly, especially if the method uses pI markers in the acidic range of the pH gradient. User Guide for Maurice, Maurice C. and Maurice S.
Page 486 What kind of pI markers can I use? User Guide for Maurice, Maurice C. and Maurice S.
Page 487 ProteinSimple offers a selection of absorbance- and fluorescence-compatible pI markers at pI 3.38, 4.05, 5.85, 6.14, 7.05, 8.40, 9.50, 9.99, and 10.17.
Page 488: Frequently Asked Questions: Ce-Sds Applications
Troubleshooting Compass for iCE lets you run a self test on Maurice, Maurice C. and Maurice S. systems. These diagnostic tests check many internal components and can help you determine if you have an instrument issue or not. Go to “Instrument Software (Embedded) Updates”...
Page 489: Cief Application Troubleshooting
< 2 μA. Electrolyte contamination Replace the anolyte and catholyte solutions in the electrolyte tanks. User Guide for Maurice, Maurice C. and Maurice S.
Page 490 Always spin samples for 10 min at 1000 xg before adding them to sample vials or wells of a 96-well plate. • Dispense the solution at the bottom of the vial/well to avoid trapping any air bubbles. User Guide for Maurice, Maurice C. and Maurice S.
Page 491: Ce-Sds Application Troubleshooting
Make sure reagent vials are placed in the right positions in the sample and reagent platform. Top Running Buffer vial is overloaded • Use a new cartridge insert and new Top Running Buffer vial. User Guide for Maurice, Maurice C. and Maurice S.
Page 492 Make sure the salt and protein concentrations of netic injection your sample are within the recommended ranges. Rising Baseline Problem Solution UV lamp is approaching the life time limit Replace the lamp. User Guide for Maurice, Maurice C. and Maurice S.
Page 493: Chapter 16: General Information
483 Chapter 16: General Information Chapter Overview • Compliance • Safety Guidelines • Consumables and Reagents • Customer Service and Technical Support • Legal Notices User Guide for Maurice, Maurice C. and Maurice S.
Page 494: Compliance
• Connect the equipment into an outlet on a circuit different from that to which the receiver is con- nected. • Consult the dealer or an experienced radio/television technician for help. User Guide for Maurice, Maurice C. and Maurice S.
Page 495: Safety Guidelines
Safety Guidelines page 485 Modifications: Any modifications made to this device that are not approved by ProteinSimple, Inc. may void the authority granted to the user by the FCC to operate this equipment. FCC ID: 2AHGG-MAURICE Safety Guidelines !WARNING! If Maurice is not used as specified by ProteinSimple, overall safety will be impaired.
Page 496: Chemical Waste Hazards
(e.g., safety glasses, gloves, or clothing). • Use precaution when emptying waste. • Dispose of waste in accordance with good laboratory practices and local, state/provincial, or national environmental and health regulations. User Guide for Maurice, Maurice C. and Maurice S.
Page 497: Waste Production And Disposal
Waste should be disposed of in accordance with good laboratory practices and local, state/provincial, or national environmental and health regulations. Safety Data Sheets Some chemicals used with Maurice may be listed as hazardous. Warnings are displayed on the labels of all chemicals when hazards exist. User Guide for Maurice, Maurice C. and Maurice S.
Page 498: Instrument Safety Labels
SDSs provide users with safety information needed to store, handle, transport and dispose of the chemicals safely. We recommend updating laboratory SDS records periodically. Safety Data Sheets for ProteinSimple reagents are available online at www.proteinsimple.com/literature or by calling (888) 607-9692. Otherwise, call the chemical manufacturer directly or visit their web site.
Page 499: Consumables And Reagents
Maurice S. systems only. Pressure screw tops with o-ring for glass reagent vials (P/N 046-017), Maurice CE-SDS 046-020 Orange Pressure 12/pk. Suitable for use with Maurice, Maurice S., Maurice C systems Caps only. Maurice CE-SDS PS-MAK02-S The CE-SDS Application Kit contains all components required to run Application Kit CE-SDS application.
Page 500 Description Maurice CE-SDS 046-014 Conditioning Solution 1 for use with CE-SDS cartridge, 20 mL. For Conditioning Solu- use with CE-SDS cartridge (PS-MSC02) on Maurice and Maurice S. tion 1 systems only. Maurice CE-SDS 046-015 Conditioning Solution 2 for use with CE-SDS cartridge, 20 mL.
Page 501: Maurice Cief Consumables, Kits And Reagents
Maurice cIEF Blue 046-332 Pressure screw tops with o-ring for glass reagent vials (P/N 046-017), Pressure Caps 12/pk. Suitable for use with Maurice, Maurice S., Maurice C systems only. Maurice cIEF PS-MDK01-C The cIEF Method Development Kit provides all the reagents and...
Page 502 Maurice cIEF pI 046-033 Maurice cIEF pI Marker - 8.4, 210 μL, lyophilized. Marker - 8.4 Maurice cIEF pI 046-047 Maurice cIEF pI Marker - 9.50, 210 μL, lyophilized. Marker - 9.50 User Guide for Maurice, Maurice C. and Maurice S.
Page 503: Maurice Systems Consumables And Reagents
Maurice S. and Maurice C. systems only. Maurice sample 046-083 Vials with integrated inserts for samples, accommodate 200 μL sam- vials with inte- ple volume. 100/pk. Suitable for use with Maurice, Maurice S. and grated inserts Maurice C. systems only. Maurice clear 046-138 Clear screw caps with round opening, 100/pk.
Page 504 Customer Service and Technical Support page 494 Use our online technical support request Email: support@proteinsimple.com Telephone: 1-408-510-5500 Toll-free (US and Canada): 1-888-607-9692, option 3 www.proteinsimple.com Address ProteinSimple 3001 Orchard Parkway San Jose, CA 95134 User Guide for Maurice, Maurice C. and Maurice S.
Page 505: Legal Notices
EXCEPT AS EXPRESSLY PROVIDED IN ANY ProteinSimple SOFTWARE LICENSE AGREEMENT OR QUOTATION, THE PRODUCTS SOLD AND SER- VICES PROVIDED BY ProteinSimple ARE PROVIDED ON AN “AS IS” AND “AS AVAILABLE” BASIS WITHOUT WARRANTY OF ANY KIND. ProteinSimple AND ITS SUPPLIERS DO NOT WARRANT THE SECURITY, PRIVACY, OR ACCURACY OF ANY DATA PROVIDED VIA THE PRODUCTS OR SERVICES, AND YOU AGREE THAT THE USE OF ANY SUCH DATA BY YOU IS AT YOUR SOLE RISK.
Page 506 (b) providing Company all information relating to the Error; (c) providing access to the Licensed Software and Customer's facility where the Licensed Software is located and informing Company of any potential hazards which may User Guide for Maurice, Maurice C. and Maurice S.
Page 507 Licensed Software. Customer may contact Company via telephone at 1-888-607-9692 or e-mail at support@proteinsimple.com during the hours of 8 a.m. (Pacific Time) and 5 p.m. (Pacific Time) Monday through Friday, excluding holidays, to report any Error. A list of standard holidays will be provided to Customer upon request. Company shall have the right to determine in its sole discretion what corrective action Company will perform to support the Licensed Software.
Page 508 Company may provide any notice to Customer by e-mail. Customer may provide notice to Company by sending an e-mail to info@proteinsimple.com or a letter by United States mail to ProteinSimple, 3040 Oakmead Village Drive, Santa Clara, CA 95051, or to such other address as Company may specify in writing by posting the new address on the Company website.

This manual is also suitable for:

Maurice c Maurice

ProteinSimple Maurice S User Manual

Let's Get Started

Chapter 2 Getting Your Lab Ready

Chapter 3 Maurice

Chapter 4 Compass for Ice Overview

Chapter 5: Cief Batches

Chapter 6: CE-SDS Batches

Running Cief Applications on

Maurice and Maurice

Chapter 8 Running CE-SDS Applications on Maurice and Maurice S

Chapter 9: Run Status

Chapter 10 Controlling Maurice, Maurice C. and Maurice S

Chapter 11: CE-SDS Data Analysis

Chapter 12 Analysis

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Summary of Contents for ProteinSimple Maurice S