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Troubleshooting
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Summary of Contents for ProteinSimple Jess
- Page 1 1 User Guide for Jess Copyright © 2018 ProteinSimple. All rights reserved. ProteinSimple 3001 Orchard Parkway San Jose, CA 95134 Toll-free in the US and Canada: (888) 607-9692 Tel: (408) 510-5500 Fax: (408) 510-5599 email: support@proteinsimple.com web: www.proteinsimple.com...
- Page 2 For research use only. Not for use in diagnostic procedures Patents and Trademarks ProteinSimple's Simple Western technology is covered by issued and pending patents in the U.S. and other countries. For more information please see http://www.proteinsimple.com/Intellectual_Property.html. ProteinSimple, the ProteinSimple logo and Jess are trademarks or registered trademarks of ProteinSimple.
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Page 3: Table Of Contents
Jess......... - Page 4 User Guide for Jess...
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Page 5: Let's Get Started
1 Chapter 1: Let’s Get Started Chapter Overview • Welcome • Simple Western Assays • Imaging a Blot User Guide for Jess... -
Page 6: Simple Western Assays
Welcome Congratulations on bringing Jess into your lab! We welcome you as a new user and are excited to be a part of your work. This user guide will provide you with information on how Simple Western assays work as well as other useful operating and installation information. -
Page 7: Getting Your Lab Ready
3 Chapter 2: Getting Your Lab Ready Chapter Overview • Introduction • Space Requirements • Physical Specifications • Electrical Requirements • Environmental Requirements • Software and Computer Requirements • General Guidelines and Information User Guide for Jess... -
Page 8: Space Requirements
Space Requirements You need a lab bench or table that can support 60 lbs (27 kg) and has enough space for both Jess and her computer. There should be sufficient clearance for both heat ventilation and to provide access if the instru- ment needs service. -
Page 9: Physical Specifications
For indoor use only. Use up to altitudes of 1524 meters (5000 feet). Table 2-2: Physical Specifications Electrical Requirements Jess requires a dedicated, grounded circuit capable of delivering the appropriate current and voltage for your country. The power requirements for select regions are listed below: Region Volts (AC) -
Page 10: Environmental Requirements
Just in case you need it, a CD containing Compass for Simple Western also comes in the box. If you don't want to analyze your data at Jess's workstation in the lab, Compass software can also be installed on a separate workstation, such as your desktop computer. - Page 11 Lifting and Moving the System: Lift Jess Correctly IMPORTANT Take all the standard precautions when lifting or moving Jess. Since Jess weighs 23 kg (50 lbs), you should not lift the instrument by yourself. Two people should lift the instrument onto the lab bench.
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Page 12: Jess
8 Chapter 3: Jess Chapter Overview • Instrument Overview User Guide for Jess... - Page 13 To open the door, make sure that you see a steady blue LED on the front, then touch the small silver touch pad on the top of Jess’s door. To close, push the door until you feel Jess pull it closed.
- Page 14 Trying to Open Door While Running (red flash): Jess can’t open the door when she’s running. • Error (steady red): Jess has detected an error. To get more information, check the Status window of the Run Summary Screen in Compass for Simple Western.
- Page 15 11 Internal Components Cartridge Holder The cartridge holder will hold: • The 25-Capillary Cartridge (PS-CC01) • The 13-Capillary Cartridge (PS-CC02) • The Blot Cartridge (PN 004-651) containing a loaded membrane Cartridge Holder (Capillary) Cartridge Holder (Blot) User Guide for Jess...
- Page 16 The plate holder holds the pre-filled plate which contains samples and assay reagents. Plate Holder Sample plates should be inserted into the plate holder so that the A1 well position is aligned with the upper left corner of the tray. A1 Position Sample Plate User Guide for Jess...
- Page 17 You can't replace any parts except the power entry fuse. !WARNING! SHOCK HAZARD Disconnect the power cord from the power input to disconnect power to the instrument. • Connect to PC - A 10/100/BASE-T Ethernet (RJ-45 connector) is used to connect Jess to a computer. User Guide for Jess...
- Page 18 NOTE: Serial numbers are used to identify individual instruments on the network. System Label A system label is also located on Jess’s rear panel. It includes the ProteinSimple location, system model, power requirements, serial number and certification markings. San Jose, CA USA...
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Page 19: Chapter 4: Operating Jess
• Before You Throw the Switch • Power Up • Running Immunoassays • Imaging a Blot • Self Test • Viewing and Changing System Properties • Viewing Error and Test Logs • Status Modes • Shutdown User Guide for Jess... -
Page 20: Running Immunoassays
Received instruction in general safety practices for laboratories. • Received instruction in specific safety practices for Jess. • Received instruction on handling of biohazards (if biohazardous materials are to be used on Jess). • Read and understood all related SDS documentation. Power Up 1. - Page 21 Assay. From the pop-up window, select the Assay Type (Chemiluminescence, Fluorescence and/or Protein Normalization), Size Range (2-40 kDa, 12-230 kDa or 66-440 kDa) and Cartridge (either the 25 or 13 Capillary Cartridge). User Guide for Jess...
- Page 22 Assay, and select the correct one. 3. Click Start. The Start Run window for immunoassays displays with the default run file name and loca- tion. Click Browse if you want to save it in a different location. User Guide for Jess...
- Page 23 To check on the time remaining, click the Run Summary tab in the upper right corner of the screen. NOTE: Jess will continue to run even if Compass for Simple Western is closed. Always check the status light to confirm whether Jess is performing a run.
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Page 24: Imaging A Blot
5. Close the instrument door by gently pushing it until you feel Jess pull it closed. Jess automatically detects the blot cartridge and displays a preview image in the Start Run window for imaging. - Page 25 2. Remove your membrane and clean the blot cartridge by rinsing it thoroughly with water. Dry with a lint- free wipe. 3. Wipe down the area under the blot holder inside the instrument using a dry lint-free wipe. User Guide for Jess...
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Page 26: Self Test
When the run stops, the Start button will reap- pear so you can start another run when you’re ready. Self Test Jess can perform a set of self tests to make sure she’s operating properly. To start the testing, select Instrument in the main menu and click Self Test. -
Page 27: Viewing Error And Test Logs
To sync the instrument clock with the computer: Click Set to PC time. Viewing Error and Test Logs From the Compass for Simple Western main menu, select Instrument and click Properties. To view a log, click Logs. User Guide for Jess... - Page 28 Viewing Error and Test Logs page 24 The table that displays shows the list of Self Test logs. You can also select Command Log Instrument Log to view and save logs related to the instrument and software. User Guide for Jess...
- Page 29 1. Make sure to remove any plates, cartridges and blots before shutting down the system. 2. Close Compass for Simple Western and shut down Jess’s computer. 3. Jess should remain on unless you think she won’t be used for more than a week. In that case, you can just turn the power off.
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Page 30: Maintenance And Troubleshooting
26 Chapter 5: Maintenance and Troubleshooting Chapter Overview • Software Updates • Instrument Software (Embedded) Updates • Maintenance • Troubleshooting User Guide for Jess... -
Page 31: Instrument Software (Embedded) Updates
Clean the Door Liner The door liner must be cleaned before every run with Jess to avoid fluorescence of contaminants during image capture. 1. Open Jess' door to reveal the door liner (glossy black finish area behind the door). -
Page 32: Troubleshooting
Troubleshooting For Jess and Simple Western assay troubleshooting information, please contact ProteinSimple Technical Support toll-free in the US and Canada at (888) 607-9692 (option 3), support@proteinsimple.com or visit http://www.proteinsimple.com/resources.html?product=simple-western. You can also contact your local Field Application Scientist for help. -
Page 33: General Information
29 Chapter 6: General Information Chapter Overview • Compliance • Safety Guidelines • Customer Service and Technical Support • Legal Notices User Guide for Jess... -
Page 34: Safety Guidelines
If Jess is not used as specified by ProteinSimple, overall safety will be impaired. !WARNING! If Jess is damaged and doesn’t function properly, stop the instrument safely and contact ProteinSimple Technical Support right away toll-free in the US and Canada at 1-888-607-9692 (option 3). - Page 35 Read and understand the Safety Data Sheets (SDSs) provided by the manufacturers of the chemicals in a waste container before you store, handle or dispose of chemical waste. • Minimize contact with chemical waste. Wear appropriate personal protective equipment when han- dling chemicals (e.g., safety glasses, gloves, clothing). User Guide for Jess...
- Page 36 32 Safety Data Sheets Some chemicals used with Jess may be listed as hazardous. Warnings are displayed on the labels of all chemicals when hazards exist. Safety Data Sheets (SDSs) provide users with safety information needed to store, handle, transport and dis- pose of the chemicals safely.
- Page 37 Customer Service and Technical Support page 33 Customer Service and Technical Support Telephone (408) 510-5500 (888) 607-9692 (toll-free in the US and Canada) (408) 510-5599 E-mail support@proteinsimple.com www.proteinsimple.com Address ProteinSimple 3001 Orchard Parkway San Jose, CA 95134 User Guide for Jess...
- Page 38 EXCEPT AS EXPRESSLY PROVIDED IN ANY ProteinSimple SOFTWARE LICENSE AGREEMENT OR QUOTATION, THE PRODUCTS SOLD AND SER- VICES PROVIDED BY ProteinSimple ARE PROVIDED ON AN “AS IS” AND “AS AVAILABLE” BASIS WITHOUT WARRANTY OF ANY KIND. ProteinSimple AND ITS SUPPLIERS DO NOT WARRANT THE SECURITY, PRIVACY OR ACCURACY OF ANY DATA PROVIDED VIA THE PRODUCTS OR SERVICES, AND YOU AGREE THAT THE USE OF ANY SUCH DATA BY YOU IS AT YOUR SOLE RISK.
- Page 39 1.10 “Site” means the facility or campus set forth in the System Quotation. 1.11 “System” means the proprietary NP1000, NP100, Simon, Sally, Peggy, Wes, Jess, Sally Sue and Peggy Sue protein analysis system or any future model or successor thereto that is provided to Customer by Company pursuant to a separate agreement between the parties (the “System Quotation”).
- Page 40 Licensed Software. Customer may contact Company via telephone toll-free in the US and Canada 1-888-607-9692 or e-mail at support@proteinsimple.com during the hours of 8:00 AM (Pacific Time) and 5:00 PM (Pacific Time) Monday through Friday, excluding holidays, to report any Error. A list of standard holidays will be provided to Customer upon request. Company shall have the right to determine in its sole discretion what corrective action Company will perform to support the Licensed Software.
- Page 41 Company may provide any notice to Customer by e-mail. Customer may provide notice to Company by sending an e-mail to info@proteinsimple.com or a letter by United States mail to ProteinSimple, 3001 Orchard Parkway, San Jose, CA 95134, or to such other address as Company may specify in writing by posting the new address on the Company website.
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