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ProteinSimple Maurice User Manual

ProteinSimple Maurice User Manual (508 pages)

Brand: ProteinSimple | Category: Laboratory Equipment | Size: 19 MB

Table of Contents

Table of Contents
3
Let's Get Started

11
- Maurice Systems
  12
- Welcome
  12
Chapter 2 Getting Your Lab Ready

13
- Introduction
  14
- Space Requirements
  14
- Physical Specifications
  15
- Electrical Requirements
  15
- Environmental Requirements
  15
- Software and Computer Requirements
  16
- General Guidelines and Information
  16
  - Intended Use
    16
  - Correctly
    16
Chapter 3 Maurice

17
- Maurice Systems
  18
- External Components
  19
  - System Door
    19
  - Status Light
    20
- Internal Components
  20
  - Cartridge Slot
    20
  - Sample and Reagent Platform
    21
  - Rear Panel
    24
  - System Labels
    25
- Computer Workstation
  26
Chapter 4 Compass for Ice Overview

28
- Launching Compass for Ice
  29
- Compass for Ice Overview
  29
  - Changing the Screen View
    30
  - Batch Screen
    30
  - Run Summary Screen
    31
  - Analysis Screen
    33
  - Screen Panes
    35
  - Title Bar
    35
  - Main Menu
    35
  - Instrument Status Bar
    35
  - Screen Tab
    36
  - View Bar
    36
  - Compass for Ice Status Bar
    36
- Software Menus
  36
  - File Menu
    37
  - Edit Menu
    37
  - View Menu
    38
  - Instrument Menu
    38
  - Window Menu
    38
  - Help Menu
    39
- Layout
  39
  - Resizing the Main Compass for Ice Window
    39
  - Resizing the Screen Tab
    40
  - Resizing Screen Panes
    40
  - Changing the Location of Screen Panes
    41
  - Layout
    41
- Software Help
  43
- Checking for and Installing New Versions of Compass
  43
- For Ice
  43
- Viewing Release Notes
  43
- Viewing the Software Log
  44
- Compass for Ice Version Information
  44
- Directory and File Information
  45
  - File Types
    46
Chapter 5: Cief Batches

48
- Batch Screen Overview
  49
  - Batch Screen Panes
    49
  - Software Menus Active in the Batch Screen
    50
- Opening a Batch
  52
- Creating a New Batch
  53
- Viewing Replicate Injections
  72
- On-Board Mixing
  73
- Batch History
  76
- Making Changes to a Batch
  77
- Viewing and Editing Batches in Completed Runs
  78
- Copying and Pasting Injection Names or Sample Ids
  79
- From Other Documents
  79
- Importing and Exporting Methods
  81
  - Importing a Method
    82
  - Exporting a Method
    82
- Batch Reports
  83
Chapter 6: CE-SDS Batches

86
- Batch Screen Overview
  87
  - Batch Screen Panes
    87
  - Software Menus Active in the Batch Screen
    88
- Opening a Batch
  90
- Creating a New Batch
  91
  - Step 1 - Open a Template Batch
    91
  - Step 2 - Assign Your Samples
    92
  - Step 3 - Assign Your Method Parameters
    95
  - Step 4 - Set up Your Injections
    97
  - Step 5 - Add Programmed Pauses and Stops
    101
  - (Optional)
    101
  - Step 6 - Add Batch Notes (Optional)
    102
  - Step 7 - Modify Default Analysis Parameters
    102
  - (Optional)
    102
  - Step 8 - Save Your Batch
    103
- Viewing Replicate Injections
  104
- Batch History
  105
- Making Changes to a Batch
  106
- Viewing and Editing Batches in Completed Runs
  107
- Copying and Pasting Injection Names or Sample Ids
  108
- From Other Documents
  108
- Importing and Exporting Methods
  110
  - Importing a Method
    111
  - Exporting a Method
    111
- Batch Reports
  112
Running Cief Applications on

116
Maurice and Maurice

116
- Before You Throw the Switch
  117
- Power up
  117
- Running Cief Applications
  117
  - What You'll Need
    117
  - Step 1: Prep Your Markers, Samples and Reagents
    118
  - Step 2: Prep the Cartridge
    122
  - Step 3: Install the Cartridge
    124
  - Step 4: Load Samples and Reagents
    125
  - Step 5: Create a Batch
    127
  - Step 6: Start the Batch
    141
- Editing a Running Batch
  143
- Post-Batch Procedures
  145
- Checking Your Data
  148
  - Step 1: Select Your Detection Mode
    148
  - Step 2: Check Your Pi Markers
    148
  - Step 3: Checking Sample Peaks
    151
  - Step 4: Assigning Peak Names
    154
Chapter 8 Running CE-SDS Applications on Maurice and Maurice S

155
- Before You Throw the Switch
  156
- Power up
  156
- Running CE-SDS Applications
  156
  - What You'll Need
    156
  - Step 1: Prep Your Internal Standard, Samples and Reagents
    157
  - Internal Standard
    157
  - Step 2: Prep the Cartridge
    163
  - Step 3: Install the Cartridge
    165
  - Step 4: Load Samples and Reagents
    167
  - Step 5: Create a Batch
    169
  - Step 6: Start the Batch
    179
- Editing a Running Batch
  181
- Post-Batch Procedures
  183
- Checking Your Data
  187
  - Step 1: Check Your Internal Standard
    187
  - Step 2: Set Your Molecular Weight (MW)
    191
  - Markers
    191
  - Step 3: Checking Sample Peaks
    195
  - Step 4: Assigning Peak Names
    198
Chapter 9: Run Status

199
- Run Summary Screen Overview
  200
  - Run Summary Screen Panes
    200
  - Software Menus Active in the Run Summary
    202
  - Screen
    202
- Opening Run Files
  204
  - Opening One Run File
    204
  - Opening Multiple Run Files
    204
- Batch Injection Information
  205
  - Injection Flags
    207
- Run Status Information
  207
- Viewing the Focus Series (Cief Only)
  209
- Viewing the Separation (CE-SDS Only)
  210
- Current and Voltage Plots
  211
- Run History
  213
  - Viewing Multiple Events
    213
  - Copying History Info
    215
- Viewing Run Errors
  215
- Injection Reports
  216
  - Example Analysis and Injection Report: Cief
    221
- Switching between Open Run Files
  223
- Closing Run Files
  224
Chapter 10 Controlling Maurice, Maurice C. and Maurice S

225
- Instrument Control
  226
  - Starting a Run
    226
  - Cleaning
    226
- Stopping a Run
  229
  - Emergency Stop
    230
- Status Modes
  230
- Shutdown
  231
- Instrument Software (Embedded) Updates
  231
- Self Test
  232
- Viewing and Changing System Properties
  233
- Checking Cartridge Status
  234
- Instrument Reports
  235
  - Example Instrument Report
    237
- Viewing Log Files
  241
  - Runs Log
    241
  - Instrument Log
    242
  - Self Test Logs
    245
  - Command Log
    247
Chapter 11: CE-SDS Data Analysis

249
- Analysis Screen Overview
  250
  - Analysis Screen Panes
    250
  - Software Menus Active in the Analysis Screen
    251
  - Opening Run Files
    253
  - Opening One Run File
    253
  - Opening Multiple Run Files
    253
- How Run Data Is Displayed
  255
- Viewing Run Data
  261
  - Switching between Samples and Standards Data
    261
  - Views
    261
  - Selecting and Displaying Injection Data
    264
  - Switching between Single and Multiple Views of
    267
  - Injections
    267
  - Hiding Injection Data
    269
- Data Notifications and Warnings
  270
- Checking Your Results
  271
- Group Statistics
  272
  - Using Groups
    272
  - Viewing Sample Injection Groups
    273
  - Viewing Statistics
    273
  - Hiding or Removing Injections in Group
    275
  - Analysis
    275
- Copying Results Tables and Graphs
  276
  - Copying Results Tables
    276
  - Copying the Graph
    276
  - Saving the Graph as an Image File
    276
- Exporting Run Files
  277
  - Exporting Results Tables
    277
  - Exporting Raw Sample Electropherogram
    277
  - Data
    277
- Changing Sample Protein Identification
  278
  - Adding or Removing Sample Data
    278
  - Hiding Sample Data
    280
  - Changing Peak Names for Sample Data
    281
- Changing the Electropherogram View
  282
  - Autoscaling the Electropherogram
    283
  - Customizing the Data Display
    283
  - Stacking Multiple Electropherograms
    289
  - Overlaying Multiple Electropherograms
    290
  - Zooming
    291
  - Selecting Data Viewing Options
    292
  - Adding and Removing Baseline Points
    296
  - Selecting the Graph X-Axis Range
    297
- Closing Run Files
  299
- Analysis Settings Overview
  300
- Advanced Analysis Settings
  302
  - Internal Standard Settings
    302
  - Advanced Analysis Settings Groups
    303
  - Creating a New Analysis Group
    303
  - Changing the Default Analysis Group
    304
  - Modifying an Analysis Group
    305
  - Deleting an Analysis Group
    305
  - Applying Analysis Groups to Specific Run Data
    306
- Markers Analysis Settings
  308
  - Markers Settings
    309
  - Markers
    309
  - Standards Analysis Settings Groups
    310
  - Creating a New Standards Group
    311
  - Changing the Default Standards Group
    314
  - Modifying a Standards Group
    314
  - Deleting a Standards Group
    314
  - Applying Standards Groups to Specific Run
    315
  - Data
    315
- Peak Fit Analysis Settings
  317
  - Range Settings
    318
  - Baseline Settings
    319
  - Peak Find Settings
    319
  - Peak Fit Analysis Settings Groups
    320
  - Creating a New Peak Fit Group
    321
  - Changing the Default Peak Fit Group
    323
  - Modifying a Peak Fit Group
    323
  - Deleting a Peak Fit Group
    324
  - Applying Peak Fit Groups to Specific Run Data
    324
- Manual Peak Integration
  327
- Peak Names Settings
  331
  - Peak Names Analysis Settings Groups
    331
  - Creating a Peak Names Group
    332
  - Modifying a Peak Names Group
    335
  - Deleting a Peak Names Group
    335
  - Applying Peak Names Groups to Run Data
    336
- Injection Reports
  338
  - Example Analysis and Injection Report
    340
- Importing and Exporting Analysis Settings
  343
  - Importing Analysis Settings
    343
  - Exporting Analysis Settings
    343
Chapter 12 Analysis

345
- Analysis Screen Overview
  347
  - Analysis Screen Panes
    347
  - Software Menus Active in the Analysis Screen
    348
- Opening Run Files
  350
  - Opening One Run File
    350
  - Opening Multiple Run Files
    350
- How Run Data Is Displayed
  352
- Viewing Run Data
  356
  - Switching between Samples and Markers Data
    356
  - Views
    356
  - Selecting and Displaying Injection Data
    359
  - Switching between Single and Multiple Views of
    362
  - Injections
    362
  - Hiding Injection Data
    364
- Data Notifications and Warnings
  365
- Checking Your Results
  366
- Group Statistics
  366
  - Using Groups
    367
  - Viewing Sample Injection Groups
    367
  - Viewing Statistics
    368
  - Hiding or Removing Injections in Group
    370
  - Analysis
    370
- Copying Results Tables and Graphs
  371
  - Copying Results Tables
    371
  - Copying the Graph
    371
  - Saving the Graph as an Image File
    371
- Exporting Run Files
  372
  - Exporting Results Tables
    372
  - Exporting Raw Sample Electropherogram
    372
  - Data
    372
- Changing Sample Protein Identification
  373
  - Adding or Removing Sample Data
    373
  - Hiding Sample Data
    375
  - Changing Peak Names for Sample Data
    376
- Changing the Electropherogram View
  377
  - Autoscaling the Electropherogram
    378
  - Customizing the Data Display
    378
  - Stacking Multiple Electropherograms
    384
  - Overlaying Multiple Electropherograms
    385
  - Overlaying Fluorescence and Absorbance
    385
  - Electropherograms
    385
  - Zooming
    387
  - Selecting Data Viewing Options
    388
  - Adding and Removing Baseline Points
    392
  - Selecting the Graph X-Axis Range
    393
- Closing Run Files
  395
- Analysis Settings Overview
  396
- Advanced Analysis Settings
  398
  - Pi Markers Settings
    398
  - Advanced Analysis Settings Groups
    399
  - Creating a New Analysis Group
    399
  - Changing the Default Analysis Group
    400
  - Modifying an Analysis Group
    401
  - Deleting an Analysis Group
    401
  - Applying Analysis Groups to Specific Run Data
    402
- Detection Settings
  405
  - Changing the Detection Method
    405
  - Changing the Detection Exposure
    406
- Peak Fit Analysis Settings
  407
  - Range Settings
    408
  - Baseline Settings
    408
  - Peak Find Settings
    408
  - Peak Fit Analysis Settings Groups
    410
  - Creating a New Peak Fit Group
    410
  - Changing the Default Peak Fit Group
    412
  - Modifying a Peak Fit Group
    412
  - Deleting a Peak Fit Group
    413
  - Applying Peak Fit Groups to Specific Run Data
    413
- Manual Peak Integration
  416
- Peak Names Settings
  421
  - Peak Names Analysis Settings Groups
    421
  - Creating a Peak Names Group
    422
  - Modifying a Peak Names Group
    425
  - Deleting a Peak Names Group
    425
  - Applying Peak Names Groups to Run Data
    426
- Pi Markers Analysis Settings
  429
  - Pi Markers Analysis Settings Groups
    429
  - Creating a New Markers Group
    430
  - Changing the Default Markers Group
    432
  - Modifying a Markers Group
    433
  - Deleting a Markers Group
    433
  - Applying Markers Groups to Specific Run Data
    434
- Injection Reports
  436
  - Example Analysis and Injection Report
    438
- Importing and Exporting Analysis Settings
  441
  - Importing Analysis Settings
    441
  - Exporting Analysis Settings
    441
Preferences

443
Chapter 13: Setting Your

444
- Customize Your Preferences
  444
- Enabling Access Control
  445
- Setting Data Export Options
  445
- Setting up Automatic Injection Reports
  446
- Selecting Custom Plot Colors for Graph Overlay
  447
- Grouping Options
  449
- Setting up Maurice Systems to Send Tweets
  450
Chapter 14 Compass Access Control and 21 CFR Part 11 Compliance

455
- Overview
  456
- Authorization Server
  456
  - Server Administration
    457
  - Adding Non-Admin Users
    458
  - Adding Admin Users
    462
  - Resetting User Passwords
    463
  - Audit Trail
    464
  - Password Policy Settings
    466
  - LDAP Settings
    466
  - Encryption Details
    467
- Enabling Access Control
  468
- Logging in to Compass for Ice
  469
  - Locking and Unlocking the Application
    470
  - Resolving Log in Issues
    470
- Saving Changes
  471
- Signing Files
  472
- Instrument Command Log
  472
- Run File History
  475
- Troubleshooting Problems and Suggested
  475
- Solutions
  475
Chapter 15 Maintenance and Troubleshooting

477
- Cartridge Handling and Care
  478
  - Cief Cartridge
    479
  - CE-SDS Cartridge
    480
- Maintenance
  481
  - Daily
    481
  - Yearly
    481
  - Changing the Fuse
    481
- Spare Parts
  483
- Software Updates
  483
- Instrument Software (Embedded) Updates
  483
- Frequently Asked Questions: Cief Applications
  484
- Frequently Asked Questions: CE-SDS Applications
  488
- Troubleshooting
  488
  - Cief Application Troubleshooting
    489
  - CE-SDS Application Troubleshooting
    491
Chapter 16: General Information

493
- Compliance
  494
- Safety Guidelines
  495
  - Chemical Hazards
    495
  - Chemical Waste Hazards
    496
  - Waste Production and Disposal
    497
  - Safety Data Sheets
    497
  - Instrument Safety Labels
    498
- Consumables and Reagents
  499
  - Maurice Cief Consumables, Kits and Reagents
    501
  - Maurice Systems Consumables and Reagents
    503
- Customer Service and Technical Support
  503
- Legal Notices
  505
  - Maurice Disclaimer of Warranty
    505
  - License Agreement
    505

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