Page 2
ProteinSimple which supplies them. ProteinSimple reserves the right, subject to any regulatory and contractual approval, if required, to make changes in specifications and features shown herein, or discontinue the product described at any time without notice or obligation. Contact your local ProteinSimple Sales Representative for the most current information.
Page 3
AlphaImager Mini ® AlphaImager Mini User Guide...
Denna symbol anger att elektriska och elektroniska utrustningar inte får avyttras som osorterat hushållsavfall och måste samlas in separat . Var god kontakta en auktoriserad tillverkarrepresentant för information angående avyttring av utrustningen. AlphaImager Mini User Guide...
• Always cover all skin that may be exposed to UV light, especially the face, neck, hands, and arms. • Always make sure that any UV protection devices are working properly. If not, discontinue use until the device(s) are properly repaired. • Please use only UV lamps in the transilluminator. AlphaImager Mini User Guide...
Page 7
Under normal conditions, the temperature of glass surface of UV transilluminator is below 50 °C and safe to touch. However, if the system malfunctions, it is possible that the glass surface temperature exceeds 80°C. Please exercise caution when touching the glass surface with your hand if this occurs. AlphaImager Mini User Guide...
• Settings adjust image but does not affect true data values in image analysis Export • Exports acquired image into AlphaView software • Dialog box will indicate if you do not have AlphaView software on your computer AlphaImager Mini User Guide...
AlphaImager Mini Package Options AlphaImager Mini Unit P/N 92-14005-00 • Camera • Custom Image-250 cabinet with mounted gasket, bracket • Step-up ring with diopter lens and Orange filter pre-installed • USB A to mini B cable • Power adapter • AlphaSnap Software...
Welcome Screen will appear. Select Next. 2. Read the AlphaSnap Software License and select I Agree, and select Next. 3. Designate the location for software installation. Default is C:\ProgramFiles\Cell Biosciences\AlphaImager Mini or C:\ProgramFiles\ProteinSimple\AlphaImager Mini. Select Next. AlphaImager Mini User Guide...
Page 11
4. Name the Program Manager Group. Default is Cell Biosciences\AlphaImager Mini or ProteinSimple\AlphaImager Mini. Select Next. 5. Select Next to start the installation process. 6. Installation in Progress *** If your computer does not yet have Microsoft .Net platform, it will prompt you for installation prior to the completion of the software installation.
Page 12
9. After restart, you will find a desktop shortcut automatically created for you, labeled AlphaImager Mini. You can double click on this to launch the program. 10. In Device Manager, you will now see the AlphaImager Mini camera appear under AIC Devices.
The completed assembly will look like the above picture. Note that the camera mounting plate is flush with the bracket edge, and that the gasket is level with the top of the step up ring. AlphaImager Mini User Guide...
Page 14
(when facing the cabinet from the front). c. Connect one end of the USB cable to any USB port on the computer and the other end to the camera port located on the top of the camera casing (mini B connector). AlphaImager Mini User Guide...
Page 15
4. Capturing Images with AlphaImager Mini and your Computer: a) Launch the AlphaImager Mini software from the desktop shortcut. b) Live will automatically be active (the button will be a burgundy color) and you will have a live preview of your sample.
If you do not have AlphaView, this option will be grayed out. If you do have AlphaView, this will be enabled. • Question Mark: This button located in the bottom left hand corner will bring up an Info box providing the name of the software, and the software version number. AlphaImager Mini User Guide...
UV 302 / 365 excitable. The Orange filter is standard; optional green, red and blue filters can be purchased from ProteinSimple. • We typically use SYBR Green, can we image that in AlphaImager Mini? Yes. In addition to the Ethidium Bromide filter which comes standard with AlphaImager Mini, optional filters (including a Green filter which is appropriate for SYBR Green) are available for purchase from ProteinSimple.
Page 18
Removal of these screws will allow the cover to be taken off. The UV light bulbs are now accessible, and can be easily swapped out. • Is there a UV safety switch? No. Since AlphaImager Mini can be sold without a UV transilluminator, there is no safety switch. We have placed a prominent UV label right by the door handle as a reminder to users to check if the UV lights are off prior to opening the door.
Check to make sure you only have the +2 diopter lens on the end of the lens and that you have removed any unnecessary filter. Interference filters can be removed by turning the filter ring counter-clockwise. AlphaImager Mini User Guide...
EBR-500K +2 Diopter Lens, 58mm 74-12066-00 Power Adapter, AC 22-12033-00 USB Cable 23-14204-00 Bracket 90-13915-00 Gasket 90-13916-00 Camera Mounting Plate 90-14049-00 Camera and mounting plate screws 41-11452-05 Bracket screws (4) 41-11449-04 Bracket screw washers (4) 41-11519-06 AlphaImager Mini User Guide...
Need help?
Do you have a question about the AlphaImager Mini and is the answer not in the manual?
Questions and answers