ProteinSimple Maurice CE-SDS Application Manual
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ProteinSimple Maurice CE-SDS Application Manual

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Maurice® CE-SDS Application Guide

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Summary of Contents for ProteinSimple Maurice CE-SDS

  • Page 1 Maurice® CE-SDS Application Guide...

  • Page 2: Storage Conditions

    Maurice clear screw caps for sample and reagent vials • Maurice CE-SDS IgG Standard, PN 046-039 Maurice glass reagent vials, 2 mL • Maurice CE-SDS Molecular Weight Markers, PN 046-432 Maurice CE-SDS orange pressure caps (optional) Maurice 96-well plates • Maurice sample vials with integrated inserts, 0.2 mL,...

  • Page 3: Application Overview

    Maurice CE-SDS Application Guide Guidelines for great results • Whenever you handle the cartridge or remove it from its packaging, make sure the cartridge inlet doesn’t • Make sure you read the entire Application Guide before come in contact with any surfaces. A damaged inlet may getting started.
  • Page 4 Maurice CE-SDS Application Guide Fail Run ProteinSimple Call ProteinSimple Step 1: reduced IgG Standard Tech Support Pass Step 2: Run sample • Make sure separation reagents are in correct vial positions Step3: Current ~25 µA? • Always use fresh reagents •...
  • Page 5 10. In the File menu, click New batch. If your Maurice runs Notes: both size and charge, select Maurice CE-SDS. See Appendices A, B and D for procedures on prepping the 25X Internal Standard and IgG Standard, batch reagents and CE-SDS 11.
  • Page 6 >85%. If you don’t see any peaks, or only see a single amorphous MIGrATION PEAK TYPE TIME (SECONDS) peak, please contact ProteinSimple Technical Support at support@proteinsimple.com. Internal Standard (reduced and non-reduced IgG) 650 – 800 Light chain (reduced IgG) 800 –...
  • Page 7 Maurice CE-SDS Application Guide Step 2: Set up and run your sample batch GENERAL CONSIDERATIONS FOR REDUCING CONDITIONS • For complete details on sample prep see Appendix A. Denature and reduce your samples with β-mercaptoethanol, following the instructions for sample •...
  • Page 8 8. In the File menu, click New batch. If your Maurice runs should be reproducible as shown in Figure 3. both size and charge, select Maurice CE-SDS. 2. The Internal Standard migration time in all samples 9. Add samples by highlighting the sample location(s) in should be <800 s.
  • Page 9 Maurice CE-SDS Application Guide RESOLUTION AND SIGNAL INTENSITY 20kDa 33kDa 55kDa 103kDa 178kDa 270kDa OPTIMIZATION Adjusting peak resolution or modifying signal intensity can be achieved by changing injection conditions. • Decreasing the injection time but keeping the same injection voltage and separation conditions will result in higher resolution, but lower signal.
  • Page 10 2 to 3 minute increments. Choose reproducibility, peak resolution or signal intensity, please contact the shortest separation time that provides acceptable your ProteinSimple Field Application Specialist or our Technical resolution and peak detection. Support team at support@proteinsimple.com.

  • Page 11: Appendix A: Sample And Standard Preparation

    Maurice CE-SDS Application Guide 6. For a reduced IgG Standard: Add 2.5 μL of 14.2 M Appendix A: Sample and standard β-mercaptoethanol and mix thoroughly by vortex. preparation For a non-reduced IgG Standard: Add 2.5 μL of 250 mM iodoacetamide and mix thoroughly by vortex.

  • Page 12: Appendix B: Reagent Preparation

    Maurice CE-SDS Application Guide 3. For reduced IgG samples: Add 2.5 μL of 14.2 M Cond. Cond. Separation Wash Solution 1 Solution 2 Water Matrix Solution β-mercaptoethanol and mix thoroughly by vortex. For non-reduced IgG samples: Add 2.5 μL of 250 mM Reagents Row P iodoacetamide and mix thoroughly by vortex.

  • Page 13: Appendix D: Ce-Sds Cartridge Preparation

    FIGUrE 6. Assembling the Top Running Buffer vial in the cartridge insert. Keep the cartridge insert in an upright position after the vial is inside it. Toll-free: (888) 607-9692 © 2018 ProteinSimple. ProteinSimple, the ProteinSimple logo, Maurice, and iCE are Tel: (408) 510-5500 trademarks and/or registered trademarks info@proteinsimple.com of ProteinSimple.

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