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Summary of Contents for ProteinSimple NanoPro 1000
- Page 1 1 NanoPro 1000 System User Guide Copyright © 2013 ProteinSimple. All rights reserved. ProteinSimple 3040 Oakmead Village Drive Santa Clara, CA 95051 Toll-free: (888) 607-9692 Tel: (408) 510-5500 Fax: (408) 510-5599 email: support@proteinsimple.com web: www.proteinsimple.com...
- Page 2 6,995,901, 6,909,459, 6,853,454, 6,271,042, 7,166,202, and other issued and pending patents in the U.S. and other countries. ProteinSimple, the ProteinSimple logo, the Alpha Innotech logo, Protein Forest, the Protein Forest logo, the AIC logo, AlphaCal, AlphaImager, AlphaPart11View, AlphaQuant, AlphaSnap, AlphaSpec,...
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Page 3: Table Of Contents
Door Interlock........40 NanoPro 1000 System Disclaimer of Warranty . . . 9 System Trays . - Page 4 Controlling the NanoPro 1000 System ....53 Starting a New Run ......53 Cleaning .
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Page 5: Chapter 1: General Information
1 Chapter 1: General Information Chapter Overview • Welcome • Simple Western Charge Separation Assays • Safety • Customer Service and Technical Support • Legal Notices NanoPro 1000 System User Guide... -
Page 6: Welcome
Chapter 1: General Information Welcome Congratulations on your purchase of the NanoPro™ 1000 system. ProteinSimple welcomes you as a new user. This user guide will cover important safety and instrument information, installation requirements, main- tenance and operating instructions. Simple Western Charge Separation Assays Simple Western charge separation assays take place in a capillary. -
Page 7: Safety
Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the manufacturer's cleanup procedures as recommended on the MSDS. • Comply with all local, state/provincial, or national laws and regulations related to chemical storage, handling, and disposal. NanoPro 1000 System User Guide... -
Page 8: Chemical Waste Hazards
(e.g., safety glasses, gloves, or clothing). • Use precaution when emptying the waste bottle. • Dispose of waste bottle contents in accordance with good laboratory practices and local, state/pro- vincial, or national environmental and health regulations. NanoPro 1000 System User Guide... -
Page 9: Waste Production And Disposal
It is the responsibility of the user to properly dispose of waste. The aqueous bulk waste stream is approxi- mately pH 8. For reference, ProteinSimple disposes of waste under the non-RCRA Hazardous Waste Liquid code 141 (buffer containing sodium chloride surfactants). A typical waste profile for one cycle (12 capillaries) is as follows: NOTE: Volumes should be adjusted accordingly to account for running of additional cycles. -
Page 10: Material Safety Data Sheets
(888) 607-9692. Otherwise, call the chemical manufacturer directly or visit their web site. Instrument Safety Labels The following safety labels are located on the NanoPro 1000 system. Each label will display a safety alert sym- bol indicating a potential safety hazard. - Page 11 Pinch Hazard !WARNING! PINCH HAZARD Do not remove the transparent front window on the NanoPro 1000 system unless instructed to do so by ProteinSimple Technical Support personnel. The window protects users from a potential pinch hazard that could be caused by the moving robot and gripper assembly.
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Page 12: Customer Service And Technical Support
Read and understood all related MSDSs. CAUTION Avoid using the NanoPro 1000 system in a manner not specified by ProteinSimple. While the system has been designed to protect the user, this protection may be impaired if the instrument is used improperly. -
Page 13: Legal Notices
EXCEPT AS EXPRESSLY PROVIDED IN ANY ProteinSimple SOFTWARE LICENSE AGREEMENT OR QUOTATION, THE PRODUCTS SOLD AND SER- VICES PROVIDED BY ProteinSimple ARE PROVIDED ON AN “AS IS” AND “AS AVAILABLE” BASIS WITHOUT WARRANTY OF ANY KIND. ProteinSimple AND ITS SUPPLIERS DO NOT WARRANT THE SECURITY, PRIVACY, OR ACCURACY OF ANY DATA PROVIDED VIA THE PRODUCTS OR SERVICES, AND YOU AGREE THAT THE USE OF ANY SUCH DATA BY YOU IS AT YOUR SOLE RISK. - Page 14 Software and Customer's facility where the Licensed Software is located and informing Company of any potential hazards which may be encountered while servicing the Licensed Software. Customer may contact Company via telephone at 1-888-607-9692 or e-mail at NanoPro 1000 System User Guide...
- Page 15 11 support@proteinsimple.com during the hours of 8 a.m. (Pacific Time) and 5 p.m. (Pacific Time) Monday through Friday, excluding holidays, to report any Error. A list of standard holidays will be provided to Customer upon request. Company shall have the right to determine in its sole discretion what corrective action Company will perform to support the Licensed Software.
- Page 16 Company may provide any notice to Customer by e-mail. Customer may provide notice to Company by sending an e-mail to info@proteinsimple.com or a letter by United States mail to ProteinSimple, 3040 Oakmead Village Drive, Santa Clara, CA 95051, or to such other address as Company may specify in writing by posting the new address on the Company website.
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Page 17: Chapter 2: Lab Requirements
13 Chapter 2: Lab Requirements Chapter Overview • Introduction • Space Requirements • Electrical Requirements • Environmental Requirements • Software and Computer Requirements • General Guidelines and Information NanoPro 1000 System User Guide... -
Page 18: Introduction
Lab Requirements Introduction This chapter will help you prepare the lab for the NanoPro 1000 system. Please complete the space, electri- cal, vacuum and environmental requirements prior to scheduling your installation. NOTE: Please wait for an authorized ProteinSimple representative to unpack and install the NanoPro 1000 system. - Page 19 2.17 Depth 0.76 2.49 Height 0.41 1.35 Table 2-1: NanoPro 1000 system dimensions. Dimension Meters Feet Width 2.13 6.98 Depth 0.91 2.99 Height 1.00 3.28 Table 2-2: Recommended space requirements for the NanoPro 1000 system. NanoPro 1000 System User Guide...
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Page 20: Electrical Requirements
16 Chapter 2: Lab Requirements Electrical Requirements The NanoPro 1000 system requires a dedicated, grounded circuit capable of delivering the appropriate cur- rent and voltage for your country. The power requirements for select countries are listed below: Region Volts (AC) -
Page 21: Software And Computer Requirements
Lifting and Moving the System: Use Proper Lifting Precautions IMPORTANT Use caution when lifting or moving the NanoPro 1000 system. The system weight is 82 kg (180 lbs). Two or more people are required to lift the system onto the laboratory bench. Proper lifting safety precautions must be taken. - Page 22 18 Chapter 2: Lab Requirements NanoPro 1000 System User Guide...
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Page 23: Chapter 3: Nanopro 1000 System
19 Chapter 3: NanoPro 1000 System Chapter Overview • Instrument Overview • Auxiliary Module • Compliance • Safety Guidelines • Physical Specifications NanoPro 1000 System User Guide... -
Page 24: Instrument Overview
NanoPro 1000 System Instrument Overview The NanoPro 1000 system’s individual hardware components are described in this section. All steps of the assay are performed in a single capillary for each sample and the system is capable of running up to 96 sam- ples per experiment. - Page 25 Instrument Overview page 21 System Doors The NanoPro 1000 system’s doors provide access to the inside of the instrument for the loading of samples, reagents and capillaries. To open the doors, first confirm that the green System Status Ready Indicator illuminated.
- Page 26 Compass software interface. Leveling Foot NOTE: If the NanoPro 1000 system must be moved from the original install location or requires leveling for any reason, please see “Leveling the Instrument” on page 64 for leveling procedures or contact ProteinSim- ple Technical Support.
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Page 27: Internal Components
The X-Z stage robot moves the head assembly to and from the sample, resource, incubator, separation and capillary discard trays during run execution. The robot also moves the pipettor, which is used to transfer flu- ids and perform washes within the instrument. NanoPro 1000 System User Guide... - Page 28 Anti-static ionizer fans are mounted on the inside of the system's right door. These fans are used to circulate ionized air inside the system. This provides a uniform static charge that aids in proper capillary transfer during run execution. NanoPro 1000 System User Guide...
- Page 29 Instrument Overview page 25 System Trays The NanoPro 1000 system utilizes five compartmental trays for capillary and reagent storage and to carry out specific phases of the assay. All trays can be opened and closed by selecting the Open Trays...
- Page 30 Catholyte (right). When using only one box of capillaries, it should be placed in the primary box location closest to the reagents. NOTE: You an also refer to the labels on the resource tray for proper insertion of reagents. NanoPro 1000 System User Guide...
- Page 31 Cold Block Sample plates should be lidded and inserted into the sample tray so that the A1 well position is aligned with the upper left corner of the cold block. A1 Position Sample Plate NanoPro 1000 System User Guide...
- Page 32 Chapter 3: NanoPro 1000 System NOTES: The NanoPro 1000 system requires that plate lids be used on sample plates. If a lid is not detected, a mes- sage will be displayed in the Start Run Wizard. Compass software will reopen the sample tray to allow the user to insert a lid.
- Page 33 The front trough is used for Anolyte and the rear trough for Catholyte. The NanoPro 1000 system will remove buffer(s) and wash the troughs after completion of the separation step. Chemiluminescent and fluorescent CCD images are taken of the separation in the capillaries through the detection window.
- Page 34 Capillaries may present a potential sharps hazard. Dispose of used capillaries in biomedical waste sharps containers. NOTE: An overfilled capillary discard tray may cause jamming of the sample tray. If this occurs, call techni- cal support at (888) 607-9692 or (408) 510-5500, option 3. NanoPro 1000 System User Guide...
- Page 35 The manifold wash station is used to wash and clean the instrument manifold after each separation and to prevent build-up of viscous material in the head. NOTE: A fresh manifold sponge should be replaced after each run or when setting up a new assay plate and experiment. NanoPro 1000 System User Guide...
- Page 36 The vacuum turns on when- ever the NanoPro 1000 system’s doors and a system tray are open at the same time. To use the vacuum, turn the valve to the on position. Turn the valve to the off position when not in use.
- Page 37 Instrument Overview page 33 Instrument - Left Panel Located on the lower left side of the NanoPro 1000 system is the serial number, power entry, power switch and connector panel. Network Circuit Serial Compressed Connection Breaker Number Vacuum Auxiliary Module...
- Page 38 Instrument - Rear Panel System Label A system label is located on the rear panel of the NanoPro 1000 system. It includes the following informa- tion: the ProteinSimple location, system model, power requirements, serial number and certification mark- ings. The serial number of the instrument can also be found on the lower, left side panel above the power entry.
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Page 39: Auxiliary Module
1.0 L. Deionized water is used by the NanoPro 1000 system to clean the troughs in the separation tray and the flu- idics in the head assembly. The waste bottle collects waste generated by the NanoPro 1000 system during run execution. -
Page 40: Removing And Installing Bottles
If this occurs, fill or empty each bottle as indicated using the procedures outlined in the next section. Removing and Installing Bottles Bottles may be removed from the auxiliary module whenever the NanoPro 1000 system is idle. To remove or install the waste or water bottle, please use the following procedure: IMPORTANT Do not attempt to remove a bottle while the NanoPro 1000 system is executing a run. - Page 41 Read and understand the Material Safety Data Sheets (MSDSs) provided by the manufacturers of the chemicals in the waste vial before you store, han- dle, or dispose of chemical waste. NanoPro 1000 System User Guide...
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Page 42: Connection Panel
Compressed Air: Compressed air is provided to the NanoPro 1000 system to power pneumatic actu- ations. The compressed air line is black. • Waste: Waste produced by the NanoPro 1000 system is emptied into the waste reservoir. This line is under vacuum when waste aspirations are being performed. •... -
Page 43: Compliance
!WARNING! If the unit is not used as specified by the manufacturer the overall safety will be impaired. !WARNING! If the unit is damaged and does not function properly, stop the unit safely and contact ProteinSimple Technical Support immediately. !WARNING! No user replaceable/serviceable parts except for the ionizer fan. -
Page 44: Door Interlock
Instrument menu in Compass software. Auxiliary Module Reservoirs IMPORTANT Do not attempt to remove water or waste reservoirs while the NanoPro 1000 system is executing a run. This may abort the run in progress. NanoPro 1000 System User Guide... -
Page 45: Physical Specifications
100-240 VAC, 50/60 Hz, self adjusting Power Consumption 650 W Operating Humidity Range 20-60% relative, non-condensing Operating Temperature Range 18-24 °C (64-75 °F) Table 3-1: Physical Specifications For indoor use only. Use up to altitudes of 1524 meters (5000 feet). NanoPro 1000 System User Guide... - Page 46 42 Chapter 3: NanoPro 1000 System NanoPro 1000 System User Guide...
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Page 47: Operating The Nanopro 1000 System
System Chapter Overview • Power Up • Starting a Run • Starting a Run • Stopping a Run • Waste and Water Bottles • Controlling the NanoPro 1000 System • System Status Modes • Shutdown NanoPro 1000 System User Guide... -
Page 48: Power Up
Chapter 4: Operating the NanoPro 1000 System Power Up 1. Turn on the computer connected directly to the NanoPro 1000 system. 2. Turn on the system’s main power switch. 3. Wait for the system to initialize. 4. Open Compass software. - Page 49 2. Click Start. This will launch the Start Run Wizard. NOTE: If the manifold was not cleaned prior to starting the run, a message indicating this will display. If this occurs, click to cancel the run and perform the manifold cleaning. NanoPro 1000 System User Guide...
- Page 50 3. Page 1: Check Water and Waste. The fluid levels in the accessory module bottles will be checked by the software. If the levels in both bottles will allow the NanoPro 1000 system to complete the run, the wizard screen will display...
- Page 51 When the primary box is depleted, the NanoPro 1000 system will automatically move to the secondary box. Discard leftover Running Buffer, Wash Buffer and Matrix Removal Buffer prior to refilling bottles for new run.
- Page 52 48 Chapter 4: Operating the NanoPro 1000 System NOTE: You an also refer to the labels on the resource tray for proper insertion of reagents. Catholyte Wash Buffer Anolyte Primary Capillary Box Secondary Capillary Box NanoPro 1000 System User Guide...
- Page 53 A1 well position is aligned with the upper left corner of the cold block. When this is complete, click Next. The sample tray will close. A1 Position NanoPro 1000 System User Guide...
- Page 54 NOTES: The NanoPro 1000 system requires that plate lids be used on sample plates. If a lid is not detected, a mes- sage will be displayed in the Start Run Wizard. Compass software will reopen the sample tray to allow the user to insert a lid.
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Page 55: Step 3 - Post-Run Procedures
2. Remove the assay plate. 3. Dispose of the assay plate and capillaries. Disposal will depend on the samples that have been assayed. If sample origins are unknown, ProteinSimple recommends that used capillaries and plates be disposed of in biohazard waste. -
Page 56: Stopping A Run
1. To stop a run, click Stop. When the run stops, instrument status will go to Not Ready and a Cleanup but- ton displays: NOTE: If a run is stopped prior to completion, the NanoPro 1000 system must perform a cleaning protocol to remove used capillaries from system trays. This is required to prepare the system for the next run. -
Page 57: Controlling The Nanopro 1000 System
Controlling the NanoPro 1000 System The instrument menu allows users to control the NanoPro 1000 system. NOTE: Instrument menu options are active only when a computer with Compass software is connected directly to the NanoPro 1000 system. -
Page 58: Cleaning
54 Chapter 4: Operating the NanoPro 1000 System Opening Trays To open any of the five trays, select Instrument and click Open Trays. The tray control window will appear: Resource Tray Sample Tray Open a tray by clicking on its button. The button will become highlighted indicating the tray is open. - Page 59 Controlling the NanoPro 1000 System page 55 Manual Clean This option is used for general manual cleaning and cleaning the manifold head. To do a manual cleaning, select Instrument and click Manual Clean. The manifold head will move to a safe position for easy access and the vacuum will turn on.
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Page 60: Cleaning After A Run Error
Additional cleaning steps are required if an error occurs that stops the run. When this happens, the red Error status light on the NanoPro 1000 system's front panel will come on (see “Status Lights” on page 22). Click on the Reset button displayed in Compass software. -
Page 61: Self Test
5. Repeat the steps above until the Running Buffer or residues are completely removed. 6. To complete the cleaning process, select Instrument and click Cleanup. Self Test The NanoPro 1000 system can perform a series of self tests to check for proper instrument performance. To start the test, select Instrument and click Self Test. -
Page 62: Viewing And Changing System Properties
58 Chapter 4: Operating the NanoPro 1000 System Viewing and Changing System Properties Select Instrument and click Properties to display system properties which include: • Name • Location • Type • Serial number • Instrument software version (firmware) •... -
Page 63: System Status Modes
The instrument status bar displays status, buttons and progress bars depending on what the NanoPro 1000 system is doing. • Ready/New Run button - The NanoPro 1000 system is ready but an assay is not loaded. Click to open an assay. •... - Page 64 60 Chapter 4: Operating the NanoPro 1000 System NanoPro 1000 System User Guide...
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Page 65: Maintenance And Troubleshooting
61 Chapter 5: Maintenance and Troubleshooting Chapter Overview • Software Updates • Maintenance • Preparation for Storage or Shipment • Spare Parts • Troubleshooting NanoPro 1000 System User Guide... -
Page 66: Software Updates
Ionizer Cleaning Items needed: Isopropyl alcohol, cleaning swab (lint and fiber-free, VWR P/N 89133-806) and the screwdriver (P/N 040-994) provided in the NanoPro 1000 system accessory kit. 1. Open the system’s right door to gain access to the ionizing fans. - Page 67 3. Remove the piece of foam on the back of the ionizer fan. 4. Using the cleaning swab, rub isopropyl alcohol over each of the 4 ionizing pins on the fan to remove any build-up. Pins Pins 5. Replace the panel and captive screw. NanoPro 1000 System User Guide...
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Page 68: Yearly
ProteinSimple recommends that an annual preventive maintenance visit be performed by an authorized ProteinSimple representative. Please contact Customer Support to schedule a visit. Leveling the Instrument This option allows the user to see if the NanoPro 1000 system is properly leveled. Select Instrument click Leveling to access this option. -
Page 69: Instrument Cleaning
3. Flush each hole located on the bottom of the manifold. Position the syringe tip into the first hole and inject 15 mL of the 5% Contrad solution. Repeat for each manifold hole. 4. Repeat step 3 with water. 5. When all flush steps are complete, click OK. NanoPro 1000 System User Guide... -
Page 70: System Decontamination
The following decontamination procedures should be performed after analysis of hazardous samples on the NanoPro 1000 system. Prior to Hazardous Sample Analysis The following procedure should be performed to prepare the NanoPro 1000 system for hazardous sample analysis: Items needed: 10% bleach solution 1. - Page 71 4. Repeat step 3 with 5% Contrad solution. 5. Repeat step 3 with water. 6. When all flush steps are complete, click OK. NOTE: Please contact Protein Simple Technical Support with any questions regarding the manifold clean- ing procedure. NanoPro 1000 System User Guide...
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Page 72: Preparation For Storage Or Shipment
Preparation for Storage or Shipment Storage ProteinSimple recommends storing the instrument if it will not be used for two weeks or longer. To prepare the system for storage: 1. Open the resource tray and remove any remaining capillaries along with the Wash, Running and Matrix Removal Buffer cups. -
Page 73: Spare Parts
Spare Parts page 69 Spare Parts For assistance with replacement parts, please contact ProteinSimple Technical Support at (888) 607-9692. Description Part Number Auxiliary Module Waste Bottle 002-611 Wrench, Combination, 1/2” - 5/8”, Open End 040-992 Wrench, Driver, Allen M2.5 040-993... - Page 74 70 Chapter 5: Maintenance and Troubleshooting NanoPro 1000 System User Guide...
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