ProteinSimple FluorChem M User Manual
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ProteinSimple FluorChem M User Manual

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Formerly Cell Biosciences
FluorChem M System User Guide
Copyright © 2011 ProteinSimple. All rights reserved.
ProteinSimple
3040 Oakmead Village Drive
Santa Clara, CA 95051
Toll-free: (888) 607-9692
Tel: (408) 510-5500
Fax: (408) 510-5599
email: support@proteinsimple.com
web: www.proteinsimple.com
FluorChem M System User Guide

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  • Page 1 1 Formerly Cell Biosciences FluorChem M System User Guide Copyright © 2011 ProteinSimple. All rights reserved. ProteinSimple 3040 Oakmead Village Drive Santa Clara, CA 95051 Toll-free: (888) 607-9692 Tel: (408) 510-5500 Fax: (408) 510-5599 email: support@proteinsimple.com web: www.proteinsimple.com FluorChem M System User Guide...
  • Page 2 6,995,901, 6,909,459, 6,853,454, 6,271,042, 7,166,202, and other issued and pending patents in the U.S. and other countries. ProteinSimple, the ProteinSimple logo, the Alpha Innotech logo, Protein Forest, the Protein Forest logo, the AIC logo, AlphaCal, AlphaImager, AlphaPart11View, AlphaQuant, AlphaSnap, AlphaSpec,...

  • Page 3: Table Of Contents

    Overview of FluorChem M System Components ..14 FluorChem M Instrument ....14 Selecting a Protocol ......38 Sample Screens .
  • Page 4 Changing Date and Time Settings....88 FluorChem M System File Information... . .67 Updating the Software ......89 Copying, Printing and Deleting Images .
  • Page 5 Digital Darkroom Remote Access ....100 Connecting to FluorChem M ....100 Downloading Image Files .
  • Page 6 FluorChem M System User Guide...

  • Page 7: Chapter 1: General Information

    1 Chapter 1: General Information Chapter Overview • Welcome • Introduction to the FluorChem M System • Safety • Customer Service and Technical Support • Legal Notices FluorChem M System User Guide...

  • Page 8: Welcome

    Wi-Fi device. The FluorChem M system comes with a cooled 8.3 megapixel CCD detector, 50-mm f/1.4 motorized fixed lens and a 10-position motorized filter wheel with 710/40 nm, 607/36 nm, 593/40 nm, and 537/26 nm emis- sion filters.

  • Page 9: Safety

    Warns users that serious physical injury can result if warning precautions are not heeded. Instrument Safety Labels The following safety labels are located on the FluorChem M system. Each label will display a safety alert sym- bol indicating a potential safety hazard.
  • Page 10 Exposure to UV radiation can cause permanent damage to the eyes and skin. UV Interlock Switch The FluorChem M system is equipped with a defeatable safety interlock switch which automatically cuts off the power to the transilluminator when the door is open. This eliminates any dangerous user exposure to UV light.

  • Page 11: Customer Service And Technical Support

    • Received instruction on handling of biohazards if biohazardous materials are to be used on the system. CAUTION Avoid using the FluorChem M system in a manner not specified by ProteinSimple. While the system has been designed to protect the user, this protection may be impaired if the instrument is used improperly.

  • Page 12: Legal Notices

    EXCEPT AS EXPRESSLY PROVIDED IN ANY ProteinSimple SOFTWARE LICENSE AGREEMENT OR QUOTATION, THE PRODUCTS SOLD AND SERVICES PROVIDED BY ProteinSimple ARE PROVIDED ON AN “AS IS” AND “AS AVAILABLE” BASIS WITHOUT WAR- RANTY OF ANY KIND. ProteinSimple AND ITS SUPPLIERS DO NOT WARRANT THE SECURITY, PRIVACY, OR ACCURACY OF ANY DATA PROVIDED VIA THE PRODUCTS OR SERVICES, AND YOU AGREE THAT THE USE OF ANY SUCH DATA BY YOU IS AT YOUR SOLE RISK.

  • Page 13: Chapter 2: Installation

    7 Chapter 2: Installation Chapter Overview • Introduction • Space Requirements • Electrical Requirements • Environmental Requirements • Software and Computer Requirements • System Installation FluorChem M System User Guide...

  • Page 14: Introduction

    Please complete the space, electrical and environmental requirements prior to installing the instrument. Space Requirements The FluorChem M system is a bench-top instrument which requires a stable laboratory bench or table capa- ble of supporting 75 lbs (34 kg). System dimensions and space requirements are as follows:...
  • Page 15 The FluorChem M system label displays the system power specifications: In addition to the electrical power requirements listed above, ProteinSimple requires that all grounded circuits terminate at the receptacles, and the receptacles must be located within 10 ft (3 m) of the instrument.

  • Page 16: Environmental Requirements

    Post-acquisition image analysis can be performed using AlphaView stand alone software. Please use the AlphaView software CD provided with the FluorChem M system to install the software on a separate computer workstation, such as a user’s desktop computer. In order to run AlphaView software, this...

  • Page 17: System Installation

    1. Unbox the system and place it on a lab bench. IMPORTANT The FluorChem M system weighs 75 lbs (34 kg). Two people are required to lift the system from the shipping container onto the laboratory bench. Lift the system by grasping the bottom of the instru- ment at the midsection on either side.
  • Page 18 12 Chapter 2: Installation FluorChem M System User Guide...
  • Page 19 13 Chapter 3: System Hardware Chapter Overview • Overview of FluorChem M System Components • Compliance • Safety Guidelines • General Guidelines and Information • System Specifications FluorChem M System User Guide...

  • Page 20: Overview Of Fluorchem M System Components

    UV Interlock Switch Integrated CCD Optics The FluorChem M system uses a scientific grade 8.3 megapixel CCD detector cooled to -25 °C and a motor- ized lens for optimum resolution and acquisition speed. The optics are fully automated and require no manual user adjustments.
  • Page 21 System Door The FluorChem M system door provides access to the inside of the instrument for loading of samples, gels and blots for imaging. To open the door, pull the door latch forward and down until it stops, then pull the door open.
  • Page 22 Exposure to UV radiation can cause permanent damage to the eyes and skin. The FluorChem M system UV transilluminator uses 302 nm bulbs. These bulbs can be replaced with 365 nm bulbs if needed. Please refer to “UV Transilluminator Bulb Replacement” on page 107 for information on replacing UV transilluminator bulbs.
  • Page 23 USB Ports The FluorChem M system has two side and one top USB port. These ports can be used with USB flash drives for copying image files or to connect directly to a compatible USB thermal printer.

  • Page 24: Sample Screens

    Disconnect power cord from the instrument power inlet to disconnect power to the instrument. Sample Screens The FluorChem M system is provided with three sample screens for imaging a wide range of applications. Sample screens are application specific and should be used as follows: •...

  • Page 25: Compliance

    Compliance page 19 When not in use, sample screens should be stored in the screen holder provided with the FluorChem M sys- tem: Compliance The FluorChem M system complies with: • EN 61010-1:2001: Safety requirements for electrical equipment for measurement, control, and labo- ratory use - Part 1: General requirements (EU) •...

  • Page 26: Safety Guidelines

    Technical Support immediately. CAUTION Avoid using the FluorChem M system in a manner not specified by ProteinSimple. While the system has been designed to protect the user, this protection may be impaired if the instrument is used improperly. UV Transilluminator CAUTION The FluorChem M system contains an integrated ultraviolet transilluminator.

  • Page 27: General Guidelines And Information

    21 General Guidelines and Information System Operation: Notification of Intended Use NOTE: The FluorChem M system is for research use only. Not for use in diagnostic or clinical procedures. Lifting and Moving the System: Use Proper Lifting Precautions IMPORTANT Use caution when lifting or moving the FluorChem M system.

  • Page 28: System Specifications

    16-bit A/D, thermoelectrically cooled to -25 °C absolute and regulated Optics 50-mm, f/1.4 motorized fixed lens Field of View 16 cm x 12 cm (fixed) Image output 16-bit PNG and 8-bit PNG For indoor use only. FluorChem M System User Guide...

  • Page 29: Chapter 4: Getting Started

    23 Chapter 4: Getting Started Chapter Overview • Powering on the System • Overview of FluorChem M User Interface • Imaging Setup • UV Light Override FluorChem M System User Guide...

  • Page 30: Powering On The System

    Getting Started Powering on the System To power up the FluorChem M system, turn on the main power switch located at the rear of the instrument. NOTE: When imaging chemiluminescent samples, allow the FluorChem M system to equilibrate for at least 30 minutes after power up.

  • Page 31: Overview Of Fluorchem M User Interface

    The FluorChem M User Interface is accessed through the touchscreen. Users can initiate acquisition, access protocols and images, and change system settings via touchscreen commands. Tabs Tabs are used to access particular screens in the FluorChem M User Interface. To view a screen, touch the screen tab. Buttons Buttons are used for direct instrument control.
  • Page 32 Use the keyboard or number pad to type in the entry. Use the key to delete an existing entry. When text entry is complete, touch the Save button. FluorChem M System User Guide...

  • Page 33: User Interface Screens

    27 User Interface Screens The FluorChem M User Interface has four main screens that are accessed through the following tabs: Dark- room, Review, Images and System Settings. Each screen can be accessed by selecting the tab on the touch- screen.
  • Page 34 Review Tab will display the Review Screen. This screen is used to view stored images and protocol information, enter or edit image details and make image enhancements. Images can also be deleted, copied and printed. FluorChem M System User Guide...
  • Page 35 Overview of FluorChem M User Interface page 29 Images Screen Selecting the Images Tab will display the Images Screen. This screen is used to browse saved images. Stored images can also be deleted and copied from this screen. FluorChem M System User Guide...
  • Page 36 Screen Saver After a period of inactivity, the FluorChem M User Interface will automatically launch a screen saver to pre- serve the lifetime of the LCD touchscreen. When the screen saver is running the screen will be blank. Simply touch the screen to exit the screen saver mode and view the user interface.

  • Page 37: Imaging Setup

    Prior to image acquisition, samples must be placed on the appropriate sample screen and positioned properly on the UV transilluminator. Sample Screens The FluorChem M system is provided with the following screens for sample imaging. The screen used depends on the application: • Black screen: Epi fluorescent, epi white and chemiluminescent imaging •...
  • Page 38 Getting Started 3. Place the screen on the UV transilluminator. 4. Close the system door. The FluorChem M system will automatically take an epi-white light image of the sample. 5. Ensure that the area of interest in the blot or gel has been captured in the image. If not, open the system door and reposition the sample in the imaging area of the sample screen as needed.

  • Page 39: Uv Light Override

    Door Open Screen will display: 2. Select the UV Light button on the touchscreen. The following UV light dialog box will display: 3. Pull out the interlock switch on the lower right corner of the system. FluorChem M System User Guide...
  • Page 40 UV light when engaging the UV interlock switch override. 4. When finished, turn off the UV transilluminator by pushing the interlock switch back in. Then select the button to exit the UV light dialog box. FluorChem M System User Guide...

  • Page 41: Chapter 5: Image Acquisition

    35 Chapter 5: Image Acquisition Chapter Overview • Overview of the Darkroom Screen • Image Acquisition • Sample Imaging FluorChem M System User Guide...

  • Page 42: Overview Of The Darkroom Screen

    Protocol Menu This menu allows users to select from a set of pre-programmed protocols. Protocol options can be viewed by selecting the Protocol Menu box. FluorChem M System User Guide...
  • Page 43 Channel Settings Menu Channel Settings Menu box allows users to view and change light, filter, exposure and resolution set- tings for each channel. These settings can be viewed by selecting the Channel Settings Menu box. FluorChem M System User Guide...

  • Page 44: Selecting A Protocol

    Selecting a Protocol Protocol selection depends on the sample being imaged. Table 5-1shows a list of pre-programmed protocols along with the light setting, filter setting and the recommended sample screen for each. FluorChem M System User Guide...

  • Page 45: Changing Channel Settings

    CH 3 Blue epi Green 537 nm Table 5-1: FluorChem M system pre-programmed protocols. Changing Channel Settings Light, filter, exposure and resolution settings for each channel used in a pre-programmed protocol can be viewed and changed by selecting the Channel Settings Menu box.
  • Page 46 NOTE: Changes to default channel settings are not permanently saved. When settings are modified, the new selections are used for image acquisition until the FluorChem M system door is opened. At that time, all channel settings will return to the default selections specified by the protocol.
  • Page 47 Auto Exposure When Auto is selected in the protocol, the FluorChem M system will take successively longer exposures dur- ing image acquisition until the optimal exposure is reached. However, users can select the Stop button at any time to save and display the last completed image exposure taken.
  • Page 48 When Manual is selected in the protocol, the FluorChem M system will take one image at the exposure time entered. To enter an exposure time, select the time box. A numeric keypad will appear to allow the user to enter a time setting.
  • Page 49 1:30 minutes or 90 seconds depending on how the time was entered: Example 3: Manual Exposure Time of 3 hours Enter 18000 for a manual exposure time of 3 hours. The time displayed will be 180 minutes: FluorChem M System User Guide...

  • Page 50: Multi-Channel Imaging

    This increased exposure time can be very significant, particularly when imaging low abundant chemiluminescent targets. Multi-Channel Imaging The FluorChem M allows multi-channel image capture on up to four channels per acquisition. Each channel can then be subsequently overlaid or stacked in the Review Screen for visualization of: •...
  • Page 51 These icons will automatically update whenever channel settings are changed. To add a channel, select the Add Channel button. Add Channel Button The new channel added will by default display the same settings as the previous channel. FluorChem M System User Guide...
  • Page 52 Change light, filter, exposure and resolution settings as desired. Continue adding channels and changing settings as needed. A total of up to four channels can be added. Once a fourth channel is added, the Add Channel button will no longer display. FluorChem M System User Guide...
  • Page 53 Button After a channel has been deleted, the remaining channels will automatically renumber accordingly. NOTE: Imaging protocols must contain at least one channel. Channel 1 in a single-channel protocol will not be available for deletion. FluorChem M System User Guide...

  • Page 54: Image Acquisition

    The channel number and exposure time will be displayed under the Image Display. When a channel uses Auto expose, the Image Display and exposure time will refresh each time a new exposure is taken during image acquisition. FluorChem M System User Guide...
  • Page 55 The acquisition indicator will display while imaging is in progress. Progress Bar Progress Bar will indicate the FluorChem M system status during image acquisition and will refresh as each exposure is taken. FluorChem M System User Guide...
  • Page 56 During or after acquisition is complete, the Title can be edited and the user, project, tags and notes for the image file can also be entered: FluorChem M System User Guide...

  • Page 57: Changing The Channel Image Display

    However, image data for any channel can be displayed by selecting the Image Show/Hide button next to Channel Settings box. The button can be toggled between Show (arrow only) and Hide (red line through arrow). Image (Hide) Image (Show) FluorChem M System User Guide...

  • Page 58: Sample Imaging

    Sample Imaging NOTES: When imaging chemiluminescent samples, allow the FluorChem M system to equilibrate for at least 30 minutes after power up. This ensures that the system optics have reached the optimum acquisition tem- perature prior to imaging. This equilibration time is not necessary if the system power remains on at all times.
  • Page 59 53 3. Place the screen on the UV transilluminator. 4. Close the system door. The FluorChem M system will take an epi-white light image of the sample auto- matically. 5. Ensure that the area of interest in the blot or gel has been captured in the image. If not, open the system door and reposition the sample in the imaging area of the sample screen as needed.
  • Page 60 Channels that use red, green or blue epi-fluorescent lights will display in color. Images from all other light sources will display in grayscale. When acquisition is complete, all channel data is processed and final image(s) will be displayed in the Review Tab which will be described in detail in the next chapter. FluorChem M System User Guide...

  • Page 61: Chapter 6: Image Review

    • Entering Image Information • Channel Image Display and Overlay • Making Image Adjustments • Image Zoom • Viewing Pixel Values • Browsing Images • FluorChem M System File Information • Copying, Printing and Deleting Images FluorChem M System User Guide...

  • Page 62: Overview Of The Review Screen

    Images are displayed overlaid by default for protocols that acquired images on multiple channels. Date and Time of Review Tab Image Acquisition File Name Image Enhancement Tools Protocol Image Review Display Channel Information Image Information Image Number Image Review Options FluorChem M System User Guide...
  • Page 63 Channel Information Channel Settings box(es) will display the individual channel settings used for image acquisition. To view light, filter, exposure and resolution settings select the Channel Settings box: FluorChem M System User Guide...
  • Page 64 Info box: NOTE: If the Auto Expose setting was used and image acquisition was stopped by the user, Auto Stopped - see protocol details will display as the title in the Info box. FluorChem M System User Guide...

  • Page 65: Entering Image Information

    The image title default is the date and time of acquisition and can be edited by the user. Enter text in the title, user, project, tags and notes fields as desired using the touchscreen keyboard. FluorChem M System User Guide...

  • Page 66: Channel Image Display And Overlay

    Settings box to Show. Next, toggle the Image Show/Hide button(s) for the remaining channels to Hide. In the example that follows, image data for only Channel 3 (blue) is shown, all other channels are hidden. FluorChem M System User Guide...

  • Page 67: Overlaying Channel Image Data

    When multiple channels are toggled to Show, the channel image data will be overlaid automatically. In the next example, image data for Channel 1 (red), Channel 2 (green) and Channel 3 (blue) are shown overlaid. FluorChem M System User Guide...

  • Page 68: Making Image Adjustments

    To adjust image brightness, select the up or down arrows. The setting control indicator will increase or decrease as adjustments are made. All images have a default setting of zero. FluorChem M System User Guide...

  • Page 69: Invert

    This exposure is selected by default. • Exposures 3 - 9: Display views of increasing exposure levels from Exposure 2. FluorChem M System User Guide...

  • Page 70: Image Zoom

    To view other areas of the enlarged image, select and drag the image until the desired viewing area is shown. To exit zooming, toggle the Zoom button to Off. FluorChem M System User Guide...
  • Page 71 Image Zoom page 65 Zoom Off 200% Zoom 400% Zoom FluorChem M System User Guide...

  • Page 72: Viewing Pixel Values

    Pixel values for other locations on the image can be viewed by selecting another pixel of interest. To exit the Pixel Viewer, select the Close button in the upper right corner of the values box. FluorChem M System User Guide...

  • Page 73: Browsing Images

    Exposures page. Once opened in AlphaView software, FluorChem M system .fcz files can be converted to .tif or .jpeg files. Copying, Printing and Deleting Images Copying Images...
  • Page 74 .fcz file and will contain the following files: • FluorChem M system .fcz file. This file can be used for image analysis in AlphaView software. • 16-bit .png image file(s). These full bitmap files are ideal for quantitation and use in print publica- tions.

  • Page 75: Printing Images

    Printing Images Images saved on the FluorChem M system can be printed to either a digital grayscale thermal USB printer or a compact color USB printer. Please contact ProteinSimple Technical Support to obtain information on com- patible printers.
  • Page 76 Image Review Channel Date and Settings Time Settings: Green, Red, Auto, 140ms 2011-1-20 10:33AM Title Project Protein Study Multiplex Western Blot Notes First experiment FluorChem M FM0203: Laboratory FCM_0203_2011-01-20_10-33-51.fcz Instrument Name File Name and Location FluorChem M System User Guide...

  • Page 77: Deleting Images

    NOTE: Images deleted from the Review Screen are moved to the Trash page and are not permanently deleted. After an image has been deleted, the Images Screen will display. For details, please refer to “Trash Page” on page 81. FluorChem M System User Guide...
  • Page 78 72 Chapter 6: Image Review FluorChem M System User Guide...

  • Page 79: Managing Stored Images

    73 Chapter 7: Managing Stored Images Chapter Overview • Overview of the Images Screen • Editing the Image Album • Trash Page FluorChem M System User Guide...

  • Page 80: Overview Of The Images Screen

    Table. View View Thumbnails Table Image List Image List displays a complete list of all image files stored on the FluorChem M system. The Image List display depends on the View Toggle button selected. FluorChem M System User Guide...
  • Page 81 List, image files are shown in a table format. Table columns include: • User, title, project and tags entered by the user • Acquisition protocol name • Date and time of image acquisition • Protocol exposure and resolution settings FluorChem M System User Guide...

  • Page 82: Paging Buttons

    Image List to the first or last page of files. NOTE: When the Image List is at the first or last page of stored image files, the Paging buttons will become inactive. FluorChem M System User Guide...

  • Page 83: Page Menu

    To select an image for review or enhancement, select a thumbnail or row in the Image List. The selected image will then display in the Review Tab. For more information on the Review Screen and making image adjustments, please refer to Chapter 6, "Image Review". FluorChem M System User Guide...

  • Page 84: Searching For Images

    When text entry is complete, select the Return button. Search results will be returned in the Album page. To clear the text in the Search box and return to a view of all files, select the Clear button. Clear Button FluorChem M System User Guide...

  • Page 85: Editing The Image Album

    List. Once images are selected, Delete Copy buttons will become active. Selected images will be designated with a check mark, and the number of images selected will display in the upper left corner of the page: FluorChem M System User Guide...

  • Page 86: Deleting Images From The Album

    Delete button. The following dialog box will display: Select the Delete button in the dialog box. NOTE: Images deleted on the Album Edit page are moved to the Trash page and are not permanently deleted. FluorChem M System User Guide...

  • Page 87: Copying Images From The Album

    Please refer to “Overview of the Images Screen” on page 74 for details on how to use this page. Editing the Trash To delete, copy or move images in this page, select the Edit button. This will display the Trash Edit page. FluorChem M System User Guide...
  • Page 88 Moving Images back to the Image Album To move one or multiple images back to the Image Album, select the image(s) in the Image List, then select Move button. The following dialog box will display: FluorChem M System User Guide...
  • Page 89 Move button in the dialog box. The image file(s) will be moved out of the Trash and back into the Image Album. Exiting Trash Selection To exit the Trash Edit page, select the Cancel button. FluorChem M System User Guide...
  • Page 90 84 Chapter 7: Managing Stored Images FluorChem M System User Guide...

  • Page 91: Chapter 8: System Settings

    • Overview of the System Settings Screen • Changing System Information • Changing Date and Time Settings • Updating the Software • System Self Tests • Creating Dark Masters • Flat Field Calibration • Changing Focus Settings • System Log FluorChem M System User Guide...

  • Page 92: Overview Of The System Settings Screen

    System Settings Screen. The system serial number is located under the instrument icon in blue text. The FluorChem M system name and location are included on image printouts and are also used to identify the instrument on the network. System name and location information can be customized by the user by clicking on the Name button.

  • Page 93: Optics Temperature

    Changing System Information page 87 Network information is located just below the version information and displays the FluorChem M system net- work status, IP and MAC addresses. NOTE: If the FluorChem M system is not connected to a local network, the...

  • Page 94: Changing Date And Time Settings

    Use the arrows at the upper left and right to change the calendar month. To change the time, select hours or minutes in the Time boxes and use the number pad to enter the new information. FluorChem M System User Guide...

  • Page 95: Updating The Software

    Save button to save the changes. Updating the Software Software updates can be transferred to the FluorChem M system using a USB flash drive or downloaded via the web. USB Updates 1. Insert the USB drive into any USB port on the instrument.

  • Page 96: Web Updates

    4. The software will perform an automatic scan of the USB drive to locate the software installer file. When prompted, follow the on-screen instructions to complete the software update. Web Updates NOTE: Web updates require that the FluorChem M system be connected to a local network with internet access. 1. Select the Update button.

  • Page 97: System Self Tests

    System Self Tests page 91 System Self Tests FluorChem M system performance can be tested to ensure key components are functioning properly. To run the system self tests or view results from previous tests, select the Test button. Results Test Log...

  • Page 98: Creating Dark Masters

    Creating Dark Masters NOTES: Dark masters are generated on the FluorChem M prior to system shipment. Dark Master files typically only need to be updated as required for system service. Dark Master file generation requires approximately 12 hours of uninterrupted system time, during which the FluorChem M may not be used for image acquisition.

  • Page 99: Flat Field Calibration

    The start and expected completion times will be shown, along with an image counter and progress bar which will update during the Dark Master file generation process. NOTE: To maintain optimal light-tight conditions, avoid opening the FluorChem M system door after start- ing Dark Master file generation.
  • Page 100 NOTE: Ensure the fluorescence flat field screen is used in this step. The black screen used for fluorescent and chemiluminescent imaging cannot be used for flat field calibration. Close the system door and select Continue. The following status screen will display. FluorChem M System User Guide...
  • Page 101 Otherwise, place the white screen used for colorimetric imaging on the UV transilluminator. Close the system door and select Continue. The following status screen will display: FluorChem M System User Guide...

  • Page 102: Changing Focus Settings

    Changing Focus Settings NOTE: The FluorChem M system optics are set to an optimal fixed focus above the sample screen and do not normally need to be changed. However, if the focus settings do need to be adjusted, use the following procedure.
  • Page 103 The Focus Screen will close and the System Settings Screen will be displayed. 7. Select the Darkroom Tab. A new white light image will be taken and displayed using the revised focus setting. FluorChem M System User Guide...

  • Page 104: System Log

    System Settings System Log To view or copy a FluorChem M system log file, select the button. The following screen will be displayed: Use the scroll bar to review the log in its entirety. To copy the log file, insert a USB drive into any USB port on...

  • Page 105: Networking And Remote Access

    99 Chapter 9: Networking and Remote Access Chapter Overview • Digital Darkroom Remote Access • Connecting to FluorChem M • Downloading Image Files • Opening Image Files in AlphaView Software FluorChem M System User Guide...

  • Page 106: Digital Darkroom Remote Access

    To connect to a networked FluorChem M system: 1. Open a web browser on a computer or device that is on the same network as the FluorChem M system. 2. Enter the URL or the current IP address for the FluorChem M system in the browser window. The sys- tem’s URL and IP address information can be found in the...
  • Page 107 IP Address 3. The browser will expand the entered URL or IP address to http://fmXXXX.local/run_viewer/ browse_runs/ or http://xx.x.x.xxx/run_viewer/browse_runs/ and display the Images Screen. 4. The full FluorChem M Digital Darkroom remote interface will now be accessible. FluorChem M System User Guide...

  • Page 108: Downloading Image Files

    Using the remote access interface is similar to using the touchscreen interface directly on the FluorChem M system except for the following functions: • Text entry. The keyboard on the remote computer or device is used for all text and numeric entry.
  • Page 109 WinZip or other extraction program. NOTES: To open .fcz files, AlphaView Stand Alone software version 3.3 or higher must be installed on the remote computer. The remote computer must be Windows-based to run AlphaView software. FluorChem M System User Guide...

  • Page 110: Opening Image Files In Alphaview Software

    NOTE: Some browsers may prompt for the location of the AlphaView software .exe file on the remote com- puter. If this occurs, select the Browse option in the browser dialog box. Browse to where AlphaView soft- ware is installed (such as Program Files\ProteinSimple\AlphaVIEW SA) and select the AIC.AlphaView.exe file.
  • Page 111 Opening Image Files in AlphaView Software page 105 The following dialog box will display. In the Save as type drop down menu, select Tiff Files (*.tif). The original file name can be changed if desired. FluorChem M System User Guide...

  • Page 112: Opening .Png Files

    To open saved or extracted .png files on the remote computer, open AlphaView software. Select File > Open. Browse to the file location and select the desired .png file. NOTE: FluorChem M .png image files can also be opened in any other program that accepts files in .png format. FluorChem M System User Guide...

  • Page 113: Maintenance And Troubleshooting

    105 Chapter 10: Maintenance and Troubleshooting Chapter Overview • Maintenance • Accessories and Spare Parts • Troubleshooting FluorChem M System User Guide...

  • Page 114: Maintenance

    NOTE: Leaving excess cleaning solution on the LCD touchscreen may cause long-term damage to the screen. System Decontamination The following decontamination procedure must be performed should the FluorChem M system come in con- tact with hazardous samples. Items needed: 10% bleach solution and a soft cloth or paper towels 1.

  • Page 115: Uv Transilluminator Bulb Replacement

    NOTE: To ensure uniform illumination, ProteinSimple recommends replacing all six UV transilluminator bulbs at the same time. 1. Power down the FluorChem M system and unplug the power cord from the system. 2. Open the system door and slide out the UV transilluminator approximately halfway.
  • Page 116 NOTE: Remove the bulb tray gently. The bulb tray electrical cord is hard-wired directly to the UV transillumi- nator and the cord cannot be disconnected. Pulling or tugging on the bulb tray may damage the cord and lead to malfunction in the UV transilluminator. FluorChem M System User Guide...
  • Page 117 9. Align the bulb tray on the middle notch of the UV transilluminator housing and slide the tray all the way back into the housing. 10. Reinstall the inner front panel and tighten the four screws until snug. 11. Reinstall the outer front panel and tighten the four screws until snug. FluorChem M System User Guide...

  • Page 118: Changing The Fuse

    Maintenance and Troubleshooting 12. Push the UV transilluminator back into the system. 13. Plug the FluorChem M power cord back into the wall socket and turn the system power on. Changing the Fuse 1. Power down the FluorChem M system and unplug the power cord from the system.

  • Page 119: Accessories And Spare Parts

    Thermal Paper, High Gloss, High Contrast, 5 rolls P-200H Thermal Paper, High Contrast, 4 rolls P-150H Spares/Service Parts 302 nm UV Replacement Bulb 75-14084-00 365 nm UV Replacement Bulb 75-14083-00 Fuse, 250 V, 6 A 011-597 FluorChem M System User Guide...

  • Page 120: Troubleshooting

    FluorChem M Upgrade to Gold Service S-FCM-UGGS Preventive Maintenance Field Service S-PMFC-FS Preventive Maintenance Depot Service S-PMFC-DS Troubleshooting Please contact ProteinSimple Technical Support at (888) 607-9692 or support@proteinsimple.com for trou- bleshooting, service or support-related items. FluorChem M System User Guide...

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