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Bruker Fourier 300 Manual
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Bruker Fourier 300 Manual

Bruker Fourier 300 Manual

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Bruker Fourier 300 Guide
Loading a sample
• Check your solvent height. For best shimming homogeneity, the sample height should be 5 cm.
• Wipe the exterior of the tube clean using a Kimwipe.
• If present, remove the cap from the top of the magnet bore.
•
• Type "ej" in the command line at the bottom of the screen to turn the lift on.
•
• Use the depth gauge to adjust the spinner height on the tube.
• Carefully place your sample into the bore. Make sure air flow is on before releasing sample!
• Type "ij" to turn off the lift.

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Summary of Contents for Bruker Fourier 300

  • Page 1 Bruker Fourier 300 Guide Loading a sample • Check your solvent height. For best shimming homogeneity, the sample height should be 5 cm. • Wipe the exterior of the tube clean using a Kimwipe. • If present, remove the cap from the top of the magnet bore.
  • Page 2 Experiment Set-up • Click on the ‘Start’ tab in the TopSpin Menu bar • • Select by clicking on it. • • Give your experiment a NAME and an experiment number (EXPNO) • Make sure your data directory (DIR) is set to C:\data\student\nmr •...
  • Page 3 • • Once you have clicked on the experiment you wish to run, be sure to click the “Set selected item in editor” button. ! Note: If you change the parameter folder to nmr\par\user, you will be presented with a much more manageable set of experiments!
  • Page 4 • Choose the correct solvent from the drop down menu next to “Set solvent”. • Enter a Title for your experiment. • Click on • Click on the ‘Acquire’ tab in the TopSpin menu bar. • • Type lock in the command line at the bottom of the screen, or click on the “Lock” button. Select your deuterated solvent from the list that appears.
  • Page 5 Acquisition • Select by clicking on it. • Click on Close if the following window appears: • Wait for the receiver gain auto-adjust (rga) procedure to finish before continuing. The message “rga: finished” will appear in the lower left corner of the screen once the receiver gain has been automatically set.
  • Page 6 Processing • Click on the ‘Process’ tab in the TopSpin Menu bar. • • Click on • NOTE: This executes a processing program including commands such as an exponential window function ‘em’, Fourier transformation ‘ft’, an automatic phase correction ‘apk’ and a baseline correction ‘abs’.
  • Page 7 • • Another option is to click the button, and type in the desired left and right ppm values. • • Spectrum intensity (vertical-zoom) can be increased/decreased by pushing the mouse scroll- wheel forwards/backwards. • You can also use the icons below: •...
  • Page 8 • The following toolbar icons can be used to manipulate the spectrum. •...
  • Page 9 !"#$%&'(#)*'+#,-% • If you are using an internal standard such as TMS (tetramethyl silane), you can set the position of a particular peak quite easily. • Zoom in on the peak whose position you wish to set. • Click on the Calib. Axis. Button. •...
  • Page 10 ./'0%.#10#-2% • Click the peak picking button on the toolbar. • Drag the cursor over the tip of a peak to select it. The green box only needs to enclose the tip of the peak—dragging the box to the baseline may cause noise to be identified as peaks. •...
  • Page 11 3-+/2*'+#,-% • Click on • Set the cursor line, starting at the left of the spectrum, to the left of the first peak to be integrated, click the left mouse button and drag the cursor line to the right of the peak, then release the mouse button.
  • Page 12 Printing • Click on the ‘Publish’ tab in the TopSpin Menu bar. • • Click on • • Click on to plot the spectrum.

  • Page 13: Advanced Topics

    Advanced Topics &*/'+#-2%#-$/+$% While in the Plot Layout mode (Publish !" Plot Layout), click on the Insert New Elements, NMR icon, and select 1D spectrum. By clicking and holding the left-hand mouse button, draw out the region where the insert will be placed.
  • Page 14 To zoom in on the insert, click on the Expand icon, then hover over the insert window until you see it highlighted in green, then zoom in on the peak(s) of interest.
  • Page 15 To show peak positions, etc. in the inset, select the inset region, then click the checkbox next to Labels under the Peaks sections. Click the blue up-triangle once you are finished. If the Browser window has been hidden from display, press Control-D to toggle its visibility.
  • Page 16 Some Useful TopSpin Commands 3-$+*45/-+%6/+47% Create dataset edc or new Turn on eject gas Insert sample and turn off eject gas Turn spin gas on ro on Load shims Open lock display lockdisp Turn lock on lock 8"7/*#5/-+%6/+47%'-9%!1:4#$#+#,-% Adjust parameters (ns, sw, d1, etc.) ased Set receiver gain Start experiment...