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Making a measurement

Anaerobic fill

Due to the small volume of the Hydro 2000µP and associated problems of remov-
ing bubbles from the system, a special fill sequence called Anaerobic fill can be
used. During an anaerobic fill the pump speed is increased in steps to a target speed.
A short pause is added between steps to allow bubbles to disperse.
To use anaerobic fill click the Anaerobic fill button on the accessory control dia-
logue's Control tab. The Anaerobic fill dialogue will appear. This contains a field
for setting the final pump speed. This is the speed the pump will run at after the fill
process has finished. During a manual measurement the last value used will be
recalled. During an SOP this will be the pump speed specified in the SOP.
For best results set the pump speed field 500rpm above the final speed required.
Once the Anaerobic fill sequence is complete the final pump speed can be reset.
Example values are given below.
The dialogue prompts the user to fill the well. Once it's full press the Go button.
As bubbles escape the level of the dispersant may drop.
Once complete, ensure that the well is approximately a quarter full, using a pipette
to remove excess dispersant if necessary.
Hydro 2000µP
Material
Glass Beads in:
Water
Oils
Alcohols
Latex in water
Pump speed (SOP)
2500rpm
3000rpm
2500rpm
2000rpm
Chapter 4
Anaerobic fill
3000rpm
3500rpm
3000rpm
2500rpm
Page 4-9

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