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Summary of Contents for Malvern NANOSIGHT NS300
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Page 1: Operating Manual
NANOSIGHT NS300 OPERATING MANUAL MAN0516-08-EN-00 January 2016... - Page 2 Worcestershire WR14 1XZ United Kingdom. Tel + [44] (0)1684-892456 Fax + [44] (0)1684-892789 www.malvern.com NanoSight, Malvern and the 'hills' logo are registered trademarks in the UK and/or other countries, and are owned by Malvern Instruments Ltd. NanoSight NS300 Operating Manual (MAN0516-08-EN-00)
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Page 3: Table Of Contents
1.3.7 R ..........................8 ETURNING QUIPMENT 1.3.8 W ............................. 9 ARRANTY 2. NANOSIGHT NS300 HARDWARE ......................10 2.1 NS300 TECHNICAL SPECIFICATIONS ...................... 10 2.1.1 NS300 I ........................10 NSTRUMENT OUSING 2.1.2 NS300 L ........................... 10... - Page 4 4.3 CARE AND MAINTENANCE ........................25 5. MOUNTING THE LASER MODULE ......................26 6. DAILY USE ..............................26 7. NANOSIGHT NS300 CONTROL SOFTWARE ..................... 27 7.1 NTA S ..........................27 OFTWARE TARTUP 7.2 C ............................
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Page 5: Introduction
Other solvents may not be compatible with wetted surfaces and should not be used without confirming suitability. If in doubt about the choice of solvent and its compatibility with any part of the device, please contact Malvern Instruments (helpdesk@malvern.com) for further information. -
Page 6: Electrical Safety
• The instrument can be used with all non-flammable water-based solvents of neutral pH. Other solvents may not be compatible and must not be used without first contacting Malvern Instruments to confirm suitability. • When using biologically or chemically hazardous sample material, it is the responsibility of the operator to determine the requisite protection for each application. -
Page 7: Servicing
Between uses of the NanoSight NS300, or for longer term storage, the unit must be cleaned and dried as described in this manual. -
Page 8: Unpacking And Initial Inspection
Inspect the shipping container when the NS300 is received. Carefully check the contents for completeness and condition. The NS300 is supplied in a specially designed packing case which should be returned to Malvern Instruments after delivery. The packing case can be kept if required, although an additional charge will be made. -
Page 9: Warranty
RMA number must be obtained from Malvern Instruments before returning any equipment, and should be clearly displayed on the return shipment and included on all subsequent correspondence. If the original shipping case is not available, shipping cases will be supplied by Malvern Instruments as required to ensure safe return of the system. -
Page 10: Nanosight Ns300 Hardware
2. NanoSight NS300 Hardware 2.1 NS300 Technical Specifications 2.1.1 NS300 Instrument Housing Size: 400 mm x 400 mm x 250 mm Weight: ~12 kg Operating temperature: 10 °C to 40 °C Humidity: Up to 80% rH at 31 °C then decreasing linearly to 50% at 40 °C... -
Page 11: Ns300 Setup
5) Start the NTA software, and confirm that no hardware detection error messages are displayed in the status panel (see also Section 7 of this manual and NanoSight NS300 NTA Software guide) 2.2.2 Laser Module Configuration Figure 2: Power connection The laser module contains a specially configured and focussed laser source, mounted within a sealed housing. -
Page 12: External Fluidic Setup
The internal tubing should be attached to the top-plate inside the casing as described in Figure 6: External NS300 the following sections for the low volume flow cell or the O-ring top-plate. Fluidic Connections NanoSight NS300 Operating Manual (MAN0516-08-EN-00) -
Page 13: Low Volume Flow Cell
B: Low volume flow cell gasket component (NTA0065 ) (NTA0066) C: LVFC assembly with PDMS chamber seal on D: LVFC assembly mounted on laser module with the underside of the gasket component sprung fastening bolts NanoSight NS300 Operating Manual (MAN0516-08-EN-00) -
Page 14: Flow Cell Tubing Connection
The LVFC is suitable for use with non-flammable, water-based solvents with neutral pH. Ethanol and other solvents may not be compatible with wetted surfaces and should not be used without confirming suitability with the listed materials. If in doubt about the choice of solvent and its compatibility with any part of the Malvern device, please contact helpdesk@malvern.com for further information. -
Page 15: Flow Cell Usage
Once the flow cell is mounted onto the laser module and the inlet tubing has been pre-rinsed with buffer or sample (See Section 3.2.1), the inlet tubing can then be connected to the flow cell manifold to load liquid into the sample chamber. NanoSight NS300 Operating Manual (MAN0516-08-EN-00) -
Page 16: Changing Samples
Section 3.2.5. When using samples which commonly adhere to the optical glass surfaces, it is more appropriate to use the O-ring top- plate, which is dissassembled and thoroughly cleaned between each measurement. NanoSight NS300 Operating Manual (MAN0516-08-EN-00) -
Page 17: Manual Cleaning Procedures
Treat all optical surfaces with the same care as would be employed with equivalent surfaces on your microscope. If the optical flat in the laser module becomes damaged, it can be replaced by Malvern Instruments (pricing on request). -
Page 18: Drying The Low-Volume Flow Cell
Once the low volume flow cell is dry, check that there are no fibers or dust particles on the gasket component seal. The flow cell is then ready for reattaching to the NS300 laser module NanoSight NS300 Operating Manual (MAN0516-08-EN-00) -
Page 19: Care And Maintenance
(Fig.7C). The flow cell is then ready for reattaching to the NS300 laser module. Figure 16: Visual of the circular seal contact areas from underneath, confirming a good connection of the gasket component to the manifold NanoSight NS300 Operating Manual (MAN0516-08-EN-00) -
Page 20: O-Ring Top Plate
In this case, the Luer ports should be removed and the tubing connected using the fittings supplied, as described in Section 4.1.3. Figure 18: O-ring Top-Plate with tubing fittings for syringe pump use NanoSight NS300 Operating Manual (MAN0516-08-EN-00) -
Page 21: O-Ring Syringe Pump Tubing Connection
Important: The O-Ring Top-plate must only be used with disposable 1ml syringes with Luer port fitting. Using syringes with larger volume than 1ml, or exceeding the maximum rated flow speed of 0.05ml per second (1ml total in 20 seconds) may result in leaking or damage to components. NanoSight NS300 Operating Manual (MAN0516-08-EN-00) -
Page 22: Loading A Sample For Use With A Syringe Pump
Note: The O-ring top-plate must always be disassembled, cleaned and dried between different samples or when changing between buffer and sample loads. The O-ring top-plate is not suitable for liquid changeover without disassembly, which can lead to significant particle carryover or sample dilution in situ. NanoSight NS300 Operating Manual (MAN0516-08-EN-00) -
Page 23: Loading The Sample Through The Syringe Pump Tubing
The O-ring top-plate is now loaded ready for use with the syringe pump. Place the syringe into the syringe pump holder and operate as described in the NanoSight Syringe Pump Operating Manual. NanoSight NS300 Operating Manual (MAN0516-08-EN-00) -
Page 24: Changing Samples
It is important to take care not to abrade or scratch the optical flat surface, or introduce particulates or contaminants onto the surface. Treat all optical surfaces with the same care as would be employed with equivalent surfaces on your microscope. Should the optical flat in the laser module become damaged, it can be replaced by Malvern (pricing on request). -
Page 25: Drying The O-Ring Top-Plate
4.3.1 O-Ring Replacement The O-rings should be replaced relatively regularly depending on the frequency of use, i.e. monthly for high usage instruments, every 3 to 6 months for lower usage instruments. O-Ring packs are available on the Malvern Instruments eStore. -
Page 26: Mounting The Laser Module
Fig 23; A: Laser module mounting / removal B: Laser module locked in position The sample is then ready for NTA measurement (See Section 7 of this manual and the NanoSight NS300 NTA Software guide). 6. Daily Use After use, the system should be flushed to clean sample from the tubing and optical surfaces. -
Page 27: Nanosight Ns300 Control Software
Run the NTA Software. NanoSi The NanoSight NTA software comes pre-installed on all computer supplied by Malvern Instruments and can be run either from the desktop icon or via the start menu (under All Programs > NanoSight). On opening the NTA Program, the following screen appears:... -
Page 28: Camera Setup
7.3 Adjusting the NS300 to obtain a video The NS300 imaging position is set-up and calibrated by Malvern. The system is designed to have good beam relocation once set-up, although small adjustments may be necessary to optimise the image. If the beam appears not to be central in the field of view on screen, i.e. -
Page 29: Thresholding Pixels (High-Sensitivity Systems Only)
Filter 2 etc. to move the selected filter into the field of view. Adjust the grey histogram (see Section 7.4) so that the image intensity scale is restricted as much as possible. Adjust the focus as necessary and begin video capture. NanoSight NS300 Operating Manual (MAN0516-08-EN-00) -
Page 30: Capture Videos
Before using the temperature controller it is imperative that all the warnings mentioned above are read and understood. NanoSight NS300 Operating Manual (MAN0516-08-EN-00) -
Page 31: Initialising
When planning a measurement, the temperature at which the measurement should be taken can be set by clicking in the ‘Target temperature’ box under the SOP tab. Type in the temperature required Tick the ‘Use Temperature Control’ button. NanoSight NS300 Operating Manual (MAN0516-08-EN-00) -
Page 32: Spare Parts List
NTA4084 Replacement O-ring top-plate luer ports straight (Pack of 10) NTA4086 Replacement O-ring top-plate luer ports angled (Pack of 10) NTA4109 Top-plate sprung fastening screws (Pack of 4) CAB0380 USB Cable NTA0380 CMOS Firewire cable NanoSight NS300 Operating Manual (MAN0516-08-EN-00)
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